NL1038953C2 - Methods and compositions to reduce animal waste parameters. - Google Patents

Methods and compositions to reduce animal waste parameters. Download PDF

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Publication number
NL1038953C2
NL1038953C2 NL1038953A NL1038953A NL1038953C2 NL 1038953 C2 NL1038953 C2 NL 1038953C2 NL 1038953 A NL1038953 A NL 1038953A NL 1038953 A NL1038953 A NL 1038953A NL 1038953 C2 NL1038953 C2 NL 1038953C2
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animal
animal waste
microbial composition
total amount
present
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Dubbelink Michael Athanasius Olde
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Mod Holding B V
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Priority to NL1038953A priority Critical patent/NL1038953C2/en
Priority to PCT/EP2012/063925 priority patent/WO2013010995A1/en
Priority to EP12746297.6A priority patent/EP2731448A1/en
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Publication of NL1038953C2 publication Critical patent/NL1038953C2/en
Priority to US14/155,921 priority patent/US20140127178A1/en

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    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K50/00—Feeding-stuffs specially adapted for particular animals
    • A23K50/30—Feeding-stuffs specially adapted for particular animals for swines
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K10/00—Animal feeding-stuffs
    • A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
    • A23K10/16—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions
    • A23K10/18—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions of live microorganisms

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Polymers & Plastics (AREA)
  • Microbiology (AREA)
  • Engineering & Computer Science (AREA)
  • Animal Husbandry (AREA)
  • Zoology (AREA)
  • Food Science & Technology (AREA)
  • Biochemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Birds (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Physiology (AREA)
  • Fodder In General (AREA)
  • Feed For Specific Animals (AREA)

Description

METHODS AND COMPOSITIONS TO REDUCE ANIMAL WASTE PARAMETERS
5 BACKGROUND OF THE INVENTION I
Intensive animal agriculture generates high volumes of animal waste and thereby has a strong environmental and economical impact. Several countries introduced legislature regarding manure production, storage and 10 disposal. For example, the Netherlands implemented the Fertilizer Law in 1986, which states that farmers are not allowed to increase the manure production above certain quotas. Above this limit, a manure producer is charged a fee. Since then, similar legislations have been 15 implemented in other countries as well. In general, quotas are expressed in amounts of ammonium (NH4-N), total nitrogen (N) and/or total phosphorus (P205). Excessive excretion of these nutrients is implicated in environmental pollution, such as ammonia emission (NH3), 20 surface water pollution, and nitrate and phosphorus leaching. In addition, ammonia emission has a negative effect on animal growth and health. Furthermore, a high dry matter content of manure increases the cost of handling and disposal.
25 The need for strategies to decrease animal waste production, ammonia, nitrogen and phosphorus content in animal waste, and ammonia emission in animal agriculture is therefore of critical importance for environmental as well as economical reasons. In countries with legislation 30 that imposes quotas on farmers, decreased manure, ammonium, nitrogen and phosphorus production per animal 1038953 2 leads to larger numbers of animals that can be housed and has therefore major economical consequences.
Current possibilities to decrease these animal waste parameters in agriculture are limited and generally 5 involve nutritional management strategies. Nutritional management methods include changes in the feed composition, addition of enzymes to the feed to increase feed utilisation and careful feeding dosage and timing. However, nutritional management is often complex and 10 generally results in higher costs due to additional farm infrastructure, higher transport costs and expensive feed additives. Even with these strategies, effects on animal waste, ammonium, nitrogen and/or phosphorus production and ammonia emission are, in general, limited. Furthermore, 15 the currently available strategies generally lead to a reduction of only one of these parameters.
For example, W02009007192 provides a bacillus spores composition that secretes high levels of phytase and that 20 is to be added to animal (e.g. pig) feed. Phytase is frequently used in monogastric animal feed to enhance phytate digestion, thereby lowering phosphorus content in animal waste. The bacillus cells of this application are mutated and selected for their rapid germination and 25 outgrowth in bile salt (simulated gut environment) and their high secretion of phytase. Since W02009007192 does not include experimental data on possible phosphorus reduction in animal waste after administering this bacillus spores composition, it is not known if and by how 30 much the phosphorus content is reduced. Furthermore, the 3 composition only targets the phosphorus uptake by the animal and is not expected to influence the other important animal waste parameters, i.e. nitrogen content, ammonium content, ammonia emission, and animal waste 5 production.
US6410305 on the other hand, focuses on the treatment of animal waste. The treatment comprises two steps: a) administering a bacterial mixture to an animal to promote 10 organic digestion, and b) adding an inoculum comprising sulfide-utilizing bacteria to waste produced by the animal. Example 4 from this application describes an experiment that focuses on step a) . Inclusion of the bacterial mixture, several lytic enzymes, Yucca schidigera 15 extracts, extruded wheat and limestone in the swine diet leads to a reductions of 43% of total solids after 90 days, compared to a reduction of 22% of total solids in the control group. Although the total solids are reduced, this method requires complex feed additives containing not 20 only a bacterial mixture, but also several enzymes, plant extracts, pretreatment of feed components and limestone. In addition, this method leads only to the reduction of the total solids and not to a reduction in the total nitrogen, total ammonium and/or total phosphorus content.
25
Therefore, an objective of the present invention is to provide a method to decrease simultaneously multiple important animal waste parameters, such as the total solids content of the animal waste, the total nitrogen 30 production, the total ammonium and ammonia production, and 4 the total phosphorus production by an animal through the administration of a dietary microbial composition to the animal.
These and other aspects of the present invention will 5 become apparent to those skilled in the art after a reading of the following description of the preferred embodiment.
5
DETAILED DESCRIPTION OF THE INVENTION
It is thus a first object of the present invention to provide the use of a microbial composition to reduce at 5 least two of the following parameters of animal waste: - the dry matter content of the animal waste, - the ammonia emission, - the total amount of ammonium present in the animal waste, 10 - the total amount of nitrogen present in the animal waste, and - the total amount of phosphorus present in the animal waste.
15 Animal waste comprises all liquids, solids and gases that are excreted by the animal; in particular animal waste comprises urine, faeces, and gases from belching and flatulence.
In a further embodiment, the present invention provides 20 the use of a microbial composition to reduce the dry matter content of the animal waste and at least one of the following parameters of the animal waste: - the ammonia emission, - the total amount of ammonium present in the animal 25 waste, - the total amount of nitrogen present in the animal waste, and - the total amount of phosphorus present in the animal waste.
6
In an even further embodiment, the present invention provides the use of a microbial composition to reduce the dry matter content of the animal waste and at least two of the following parameters of the animal waste: 5 - the ammonia emission, - the total amount of ammonium present in the animal waste, - the total amount of nitrogen present in the animal waste, and 10 - the total amount of phosphorus present in the animal waste.
In an even further embodiment, the present invention provides the use of a microbial composition to reduce the dry matter content of the animal waste and at least three 15 of the following parameters of the animal waste: - the ammonia emission, - the total amount of ammonium present in the animal waste, - the total amount of nitrogen present in the animal 20 waste, and - the total amount of phosphorus present in the animal waste.
In particular, the present invention provides the use of a microbial composition to reduce the dry matter content of 25 the animal waste, the ammonia emission, the total amount of ammonium present in the animal waste, the total amount of nitrogen present in the animal waste, and the total amount of phosphorus present in the animal waste.
In a further embodiment, the use of a microbial 7 composition according to the invention comprises administering said composition to an animal. In a particular embodiment, said composition is administered to said animal by adding it to the diet. In another 5 particular embodiment, said composition is administered by nebulization. In yet another particular embodiment, said composition is administered by the combination of nebulization and adding it to the diet.
In principle, said animal can be any animal, but 10 typically, said animal is a farm animal or a pet. In particular, said farm animal is selected from the group comprising a pig, a sheep, a goat, a cow, a horse, a chicken, a duck, a goose, a turkey, and a rabbit. More in particular said farm animal is a pig.
15 In a further embodiment, the microbial composition for use in the methods of the present invention comprises bacteria. In an even further embodiment, said microbial composition comprises one or more species of Bacillus. In a particular embodiment, the microbial component of said 20 composition consists of one or more species of Bacillus.
When Bacillus species are used in the microbial composition according to the present invention, said species are particularly selected from Bacillus amyloliquefaciens, Bacillus megaterium, Bacillus subtilis, 25 Bacillus licheniformis, and Bacillus pumilus. In particular, said Bacillus species consist of Bacillus amyloliquefaciens, Bacillus subtilis, Bacillus licheniformis, and Bacillus pumilus. In another embodiment, said Bacillus species are present in the 30 microbial composition in roughly equal amounts. Therefore, 8 said Bacillus species may consist of about 25% Bacillus amyloliquefaciens, about 25% Bacillus subtilis, about 25% Bacillus licheniformis, and about 25% Bacillus pumilus.
As known by the skilled person, microbial compositions are 5 generally made by fermentation. Microbial cells may be present as vegetative cells or spores. When the composition comprises spores, the obtained spore cells are generally concentrated, dried, mixed with a carrier and packed into a suitable container.
10
It is a further objective of the present invention to provide a method to reduce at least two of the following parameters of the animal waste: - the dry matter content of the animal waste, 15 - the ammonia emission, - the total amount of ammonium present in the animal waste, - the total amount of nitrogen present in the animal waste, and 20 - the total amount of phosphorus present in the animal waste; said method comprising administering a microbial composition to an animal.
25 In another embodiment the present invention provides a method to reduce the dry matter content of the animal waste and at least one of the following parameters of the animal waste: - the ammonia emission, 30 - the total amount of ammonium present in the animal 9 waste, - the total amount of nitrogen present in the animal waste, and - the total amount of phosphorus present in the animal 5 waste; said method comprising administering a microbial composition to an animal.
In another embodiment the present invention provides a 10 method to reduce the dry matter content of the animal waste and at least two of the following parameters of the animal waste: - the ammonia emission, - the total amount of ammonium present in the animal 15 waste, - the total amount of nitrogen present in the animal waste, and - the total amount of phosphorus present in the animal waste; 20 said method comprising administering a microbial composition to an animal.
In another embodiment the present invention provides a method to reduce the dry matter content of the animal 25 waste and at least three of the following parameters of the animal waste: - the ammonia emission, - the total amount of ammonium present in the animal waste, 30 - the total amount of nitrogen present in the animal 10 waste, and - the total amount of phosphorus present in the animal waste; said method comprising administering a microbial 5 composition to an animal.
In a further embodiment the present invention provides a method to reduce the dry matter content of animal waste, the ammonia emission, the total amount of ammonium present 10 in the animal waste, the total amount of nitrogen present in the animal waste, and the total amount of phosphorus present in the animal waste; comprising administering a microbial composition to an animal.
The microbial composition used in any one of the 15 aforementioned methods is administered to the animal as defined hereinbefore.
In analogy with the foregoing uses, the animals in any one of the aforementioned methods can be any animal, but is typically a farm animal or a pet. In particular said farm 20 animal is selected from the group comprising a pig, a sheep, a goat, a cow, a horse, a chicken, a duck, a goose, a turkey, and a rabbit. More in particular said farm animal is a pig.
Said microbial composition in any one of the methods 25 according to the present invention, include each of the embodiments as defined hereinbefore
EXAMPLES
The invention will be better understood with reference to 30 the following non-limiting examples; the examples are for 11 the purpose of describing a preferred embodiment of the invention and are not intended to limit the invention thereto.
5 EXAMPLE 1:
Effect of nebulization on ammonia emission and on ammonium, nitrogen, and phosphorus content of animal waste 10 A. Experimental setup
Pigs were allocated to two separate compartments, 82 pigs to a control compartment and 123 pigs to a treatment compartment. Pigs in the treatment compartment were 15 handled identically to the pigs in the control compartment, but were administered a supplementary microbial composition through nebulization. The microbial composition consisted of approximately equal amounts of spores from Bacillus amyloliquefaciens, Bacillus subtilis, 20 Bacillus licheniformis, and Bacillus pumilus. This composition was dissolved in water to obtain a solution of approximately 7% (w/v). Every two hours part of the solution was nebulized during 30 seconds. The total amount of nebulized bacteria was 109 colony-forming units (CFU) 25 per animal per day. Pigs in the control and treatment compartment received the same standard pig feed. Until 25 kg, pigs received the following feed: barley, wheat, wheat bran, soy, corn, potato, flax, calcium carbonate, sodium chloride, and calcium sodium phosphate; to which vitamins, 30 minerals, xylanase and phytase were added. After reaching 12 25 kg, pigs received: soy, wheat, wheat bran, calcium carbonate, monocalcium phosphate, sodium chloride; to which vitamins, minerals, and phytase were added.
The ammonia concentration in the compartments was measured 5 a first time when the animals reached 45 kg and a second time when the animals reached 85 kg. During the ammonia measurements with pigs of 85 kg, the ventilation in the treatment compartment was lower than in the control compartment (45% ventilation rate compared to 60%). After 10 140 days, when the animals reached slaughter weight, several parameters of the animal waste were determined in an independent testing facility using standard analyses. The dry matter content of the manure was determined according to the procedure described in VDLUFA Bd. II. 1, 15 11.5.1; the total ammonium content (NH4-N) of the manure was determined using the procedure in DIN 38406 (E-51); the total phosphorus content (P205) of the manure was determined using the procedure described in ISO 11885:2009; and the total nitrogen content (N) of the 20 manure was determined using the procedure of VDLUFA Bd.
II.1, 3.5.2.7.
B. Results
Weight of Control Treatment Ammonia pigs (kg) compartment compartment emission ammonia (ppm) ammonia (ppm) reduction 45 26.7 26.7 19.8% 85 49.0 23.0 53.0% 25 Thus, significant reductions of ammonia emissions were 13 observed. Despite the lower ventilation rate in the treatment compartment for the pigs of 85 kg, there was still a large reduction of 53%. In addition, during both experiments, the researchers observed a markedly better 5 smell in the treatment compartments.
Similar experiments were also carried out in which the same microbial composition was sprayed on the floor of the compartment instead of nebulized in the compartment. In these experiments, no reduction of ammonia emissions was 10 observed. This indicates that to be effective, the microbial composition needs to be administered to the animal and not to the housing of the animal.
Manure parameter Control Treatment Reduction (% w/v) (% w/v) (%)
Dry matter 8.2 7.4 9.8
Ammonium (NH4-N) 0.63 0.20 68.3
Phosphorus (P205) 0.47 0.40 14.9
Nitrogen (N) 0.85 0.76 10.6 15 EXAMPLE 2:
Effect of dietary supplementation on dry matter, ammonium, nitrogen and phosphorus content of animal waste 20 A. Experimental setup
In this experiment, 144 pigs were treated during 138 days. Pigs were fed the same standard pig diet as in experiment 1. In addition, the pigs received a dietary supplement of 14 spores from Bacillus amyloliquefaciens, Bacillus subtilis, Bacillus licheniformis, and Bacillus pumilus in approximately equal amounts, mixed with a carrier (lactose). This microbial composition was added to the 5 feed at 4.107 CFÜ per day per pig. After 138 days, animal waste parameters were determined as in experiment 1; therefore obtained results can also be compared to the control of this experiment.
10 B. Results
Manure parameter Control Treatment Reduction (% w/v) (% w/v) (%)
Dry matter 8.2 5.1 37.8
Ammonium (NH4-N) 0.63 0.43 31.7
Phosphorus (P205) 0.47 0.33 29.8
Nitrogen (N) 0.85 0.57 32.9 EXAMPLE 3: 15
Effect of spraying and dietary supplementation on dry matter, ammonium, nitrogen and phosphorus content of animal waste 20 A. Experimental setup 216 piglets of 7 kg received the same standard diet for pigs under 25 kg as described above. Their diet was supplemented with the same microbial composition as in 15 experiment 2; each piglet received 2.107 CFU of this composition per day. In addition, the microbial composition of experiment 1 was sprayed on the floor of the compartment in an amount of 10.109 CFU/m2 per week. 5 After 26 days of treatment, animal waste parameters were determined as in experiment 1. These measurements were compared to averages from a database of German piglet waste parameters.
10 B. Results
Manure parameter Control Treatment Reduction (% w/v) (% w/v) (%)
Dry matter 6.5 2.9 55
Ammonium (NH4-N) 0.30 0.27 10.0
Phosphorus (P205) 0.21 0.10 51.4
Nitrogen (N) 0.40 0.33 16.5 1038953

Claims (21)

1. Het gebruik van een microbiële compositie om ten minste twee van de volgende parameters van dierlijk 5 afval te verminderen; • de ammonia-emissie, • het droge stofgehalte van het dierlijk afval, • de totale hoeveelheid ammonium aanwezig in het dierlijk afval, 10. de totale hoeveelheid stikstof aanwezig in het dierlijk afval, en • de totale hoeveelheid fosfor aanwezig in het dierlijk afval.1. The use of a microbial composition to reduce at least two of the following animal waste parameters; • the ammonia emission, • the dry matter content of the animal waste, • the total amount of ammonium present in the animal waste, 10. the total amount of nitrogen present in the animal waste, and • the total amount of phosphorus present in the animal waste. 2. Het gebruik van een microbiële compositie voor het verminderen van het droge stofgehalte van dierlijk afval en ten minste één van de volgende parameters van dierlijk afval; • de ammonia-emissie, 20. de totale hoeveelheid ammonium aanwezig in het dierlijk afval, • de totale hoeveelheid stikstof aanwezig in het dierlijk afval, en • de totale hoeveelheid fosfor aanwezig in het 25 dierlijk afval.2. The use of a microbial composition for reducing the dry matter content of animal waste and at least one of the following animal waste parameters; • the ammonia emission, 20. the total amount of ammonium present in the animal waste, • the total amount of nitrogen present in the animal waste, and • the total amount of phosphorus present in the animal waste. 3. Het gebruik van een microbiële compositie voor het verminderen van het droge stofgehalte van dierlijk afval en ten minste twee van de volgende parameters 1038953 van dierlijk afval; • de ammonia-emissie, • de totale hoeveelheid ammonium aanwezig in het dierlijk afval, 5. de totale hoeveelheid stikstof aanwezig in het dierlijk afval, en • de totale hoeveelheid fosfor aanwezig in het dierlijk afval.3. The use of a microbial composition for reducing the dry matter content of animal waste and at least two of the following parameters 1038953 of animal waste; • the ammonia emission, • the total amount of ammonium present in the animal waste, 5. the total amount of nitrogen present in the animal waste, and • the total amount of phosphorus present in the animal waste. 4. Het gebruik van een microbiële compositie voor het verminderen van het droge stofgehalte van dierlijk afval en ten minste drie van de volgende parameters van dierlijk afval te verminderen; • de ammonia-emissie, 15. de totale hoeveelheid ammonium aanwezig in het dierlijk afval, • de totale hoeveelheid stikstof aanwezig in het dierlijk afval, en • de totale hoeveelheid fosfor aanwezig in het 20 dierlijk afval.4. The use of a microbial composition for reducing the dry matter content of animal waste and reducing at least three of the following animal waste parameters; • the ammonia emission, 15. the total amount of ammonium present in the animal waste, • the total amount of nitrogen present in the animal waste, and • the total amount of phosphorus present in the animal waste. 5. Het gebruik van een microbiële compositie voor het verminderen van het droge stofgehalte, de ammonia-emissie, de totale hoeveelheid ammonium aanwezig in 25 het dierlijk afval, de totale hoeveelheid stikstof aanwezig in het dierlijk afval, en de totale hoeveelheid fosfor aanwezig in het dierlijk afval.5. The use of a microbial composition for reducing the dry matter content, the ammonia emission, the total amount of ammonium present in the animal waste, the total amount of nitrogen present in the animal waste, and the total amount of phosphorus present in the animal waste animal waste. 6. Het gebruik van een microbiële compositie volgens eender welke van claims 1 tot 5, waarin de microbiële compositie wordt toegediend aan een dier.The use of a microbial composition according to any of claims 1 to 5, wherein the microbial composition is administered to an animal. 7. Het gebruik van een microbiële compositie volgens 5 claim 6, waarin de microbiële compositie wordt toegediend aan het dier door het toe te voegen aan het dieet.The use of a microbial composition according to claim 6, wherein the microbial composition is administered to the animal by adding it to the diet. 8. Het gebruik van een microbiële compositie volgens 10 claim 6, waarin de microbiële compositie wordt toegediend aan het dier door verneveling.8. The use of a microbial composition according to claim 6, wherein the microbial composition is administered to the animal by spraying. 9. Het gebruik van een microbiële compositie volgens claim 6, waarin de microbiële compositie wordt 15 toegediend aan het dier door de combinatie van verneveling en toevoeging aan het dieet.9. The use of a microbial composition according to claim 6, wherein the microbial composition is administered to the animal through the combination of atomization and addition to the diet. 10. Het gebruik van een microbiële compositie volgens eender welke van claims 6 tot 9, waarin het 20 dier een boerderijdier of een huisdier is.10. The use of a microbial composition according to any of claims 6 to 9, wherein the animal is a farm animal or a pet. 11. Het gebruik van een microbiële compositie volgens claim 10, waarin het boerderijdier geselecteerd is uit de groep een varken, een schaap, 25 een geit, een koe, een paard, een kip, een eend, een gans, een kalkoen, en een konijn bevat.11. The use of a microbial composition according to claim 10, wherein the farm animal is selected from the group consisting of a pig, a sheep, a goat, a cow, a horse, a chicken, a duck, a goose, a turkey, and a contains rabbit. 12. Het gebruik van een microbiële compositie volgens eender welke van claims 1 tot 11, waarin de 30 microbiële compositie bacteriën bevat.12. The use of a microbial composition according to any of claims 1 to 11, wherein the microbial composition contains bacteria. 13. Het gebruik van een microbiële compositie volgens eender welke van claims 1 tot 12, waarin de microbiële compositie één of meerdere Bacillus- 5 species bevat.The use of a microbial composition according to any of claims 1 to 12, wherein the microbial composition contains one or more Bacillus species. 14. Het gebruik van een microbiële compositie volgens eender welke van claims 1 tot 12, waarin de microbiële component van de compositie bestaat uit 10 één of meerdere Bacillus-species.14. The use of a microbial composition according to any of claims 1 to 12, wherein the microbial component of the composition consists of one or more Bacillus species. 15 Bacillus megaterium, Bacillus subtilis, Bacillus licheniformis, en Bacillus pumilus bevatten.Bacillus megaterium, Bacillus subtilis, Bacillus licheniformis, and Bacillus pumilus. 15. Het gebruik van een microbiële compositie volgens claims 13 of 14, waarin de Bacillus-species geselecteerd zijn uit Bacillus amyloliquefaciens,The use of a microbial composition according to claims 13 or 14, wherein the Bacillus species are selected from Bacillus amyloliquefaciens, 16. Het gebruik van een microbiële compositie volgens claims 13 of 14, waarin de Bacillus-species 20 bestaan uit Bacillus amyloliquefaciens, Bacillus subtilis, Bacillus licheniformis, en Bacillus pumilus.16. The use of a microbial composition according to claims 13 or 14, wherein the Bacillus species consist of Bacillus amyloliquefaciens, Bacillus subtilis, Bacillus licheniformis, and Bacillus pumilus. 17. Het gebruik van een microbiële compositie 25 volgens claim 16, waarin the Bacillus-species bestaan uit ongeveer 25% Bacillus amyloliquefaciens, ongeveer 25% Bacillus subtilis, ongeveer 25% Bacillus licheniformis, en ongeveer 25% Bacillus pumilus.17. The use of a microbial composition according to claim 16, wherein the Bacillus species consist of about 25% Bacillus amyloliquefaciens, about 25% Bacillus subtilis, about 25% Bacillus licheniformis, and about 25% Bacillus pumilus. 18. Een methode voor het verminderen van het droge stofgehalte van dierlijk afval, de totale hoeveelheid stikstof aanwezig in het dierlijk afval, de totale hoeveelheid ammonium in het dierlijk afval, en de 5 totale hoeveelheid fosfor aanwezig in het dierlijk afval; bevattende het toedienen van een microbiële compositie aan een dier.18. A method for reducing the dry matter content of animal waste, the total amount of nitrogen present in the animal waste, the total amount of ammonium in the animal waste, and the total amount of phosphorus present in the animal waste; containing administering a microbial composition to an animal. 19. De methode volgens claim 18, waarin de 10 microbiële compositie aan het dier wordt toegediend zoals gedefinieerd in eender welke van claims 7 tot 9.19. The method according to claim 18, wherein the microbial composition is administered to the animal as defined in any of claims 7 to 9. 20. De methode volgens claim 18, waarin het dier een 15 boerderijdier of een huisdier is; vooral een boerderijdier geselecteerd uit de groep die een varken, een schaap, een geit, een koe, een paard, een kip, een eend, een gans, een kalkoen, en een konijn bevat.20. The method of claim 18, wherein the animal is a farm animal or a pet; especially a farm animal selected from the group that contains a pig, a sheep, a goat, a cow, a horse, a chicken, a duck, a goose, a turkey, and a rabbit. 21. De methode volgens claim 18, waarin de microbiële compositie gedefinieerd is zoals in eender welke van claims 12 tot 17. 1038953The method of claim 18, wherein the microbial composition is defined as in any of claims 12 to 17. 1038953
NL1038953A 2011-07-15 2011-07-15 Methods and compositions to reduce animal waste parameters. NL1038953C2 (en)

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NL1038953A NL1038953C2 (en) 2011-07-15 2011-07-15 Methods and compositions to reduce animal waste parameters.
PCT/EP2012/063925 WO2013010995A1 (en) 2011-07-15 2012-07-16 Methods and compositions to reduce animal waste parameters
EP12746297.6A EP2731448A1 (en) 2011-07-15 2012-07-16 Methods and compositions to reduce animal waste parameters
US14/155,921 US20140127178A1 (en) 2011-07-15 2014-01-15 Methods and compositions to reduce animal waste parameters

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Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6410305B1 (en) * 1997-08-04 2002-06-25 Biosun Systems Corporation Treatment of animal waste
CA2555516A1 (en) * 2006-07-27 2008-01-27 Noel Gauthier Food additive for human and animal and uses thereof

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6410305B1 (en) * 1997-08-04 2002-06-25 Biosun Systems Corporation Treatment of animal waste
CA2555516A1 (en) * 2006-07-27 2008-01-27 Noel Gauthier Food additive for human and animal and uses thereof

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