MY190479A - A two-dimensional electrophoresis method for separation of protein compounds - Google Patents

A two-dimensional electrophoresis method for separation of protein compounds

Info

Publication number
MY190479A
MY190479A MYPI2015702656A MYPI2015702656A MY190479A MY 190479 A MY190479 A MY 190479A MY PI2015702656 A MYPI2015702656 A MY PI2015702656A MY PI2015702656 A MYPI2015702656 A MY PI2015702656A MY 190479 A MY190479 A MY 190479A
Authority
MY
Malaysia
Prior art keywords
fractions
separation
eluted
liquid phase
dimension
Prior art date
Application number
MYPI2015702656A
Inventor
Nazri Bin Ismail Mohd
Soon Hong Kwan
Original Assignee
Univ Sains Malaysia
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Sains Malaysia filed Critical Univ Sains Malaysia
Priority to MYPI2015702656A priority Critical patent/MY190479A/en
Priority to PCT/MY2016/050030 priority patent/WO2017026890A1/en
Publication of MY190479A publication Critical patent/MY190479A/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/24—Extraction; Separation; Purification by electrochemical means
    • C07K1/26—Electrophoresis
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/24—Extraction; Separation; Purification by electrochemical means
    • C07K1/26—Electrophoresis
    • C07K1/28—Isoelectric focusing
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44756—Apparatus specially adapted therefor
    • G01N27/44773—Multi-stage electrophoresis, e.g. two-dimensional electrophoresis

Landscapes

  • Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Electrochemistry (AREA)
  • Organic Chemistry (AREA)
  • Biophysics (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biochemistry (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Chemical & Material Sciences (AREA)
  • Peptides Or Proteins (AREA)
  • Electrostatic Separation (AREA)

Abstract

The present invention relates to a method of separating protein compounds from a sample based on inverted two-dimensional polyacrylamide gel electrophoresis (i2D- PAGE) comprising the steps of: electrophoretically separating compounds from a sample loaded into a polyacrylamide gel tube in a first dimension into discrete fractions of different molecular weights, in which the separation is conducted using gel eluted liquid fraction entrapment electrophoresis whereby each of the fractions being eluted from the gel tube is trapped in a subsequent liquid phase; collecting the liquid phase containing each of the eluted fractions; and subjecting the liquid phase to a second separation in a second dimension using an isoelectric focusing medium in the presence of electric field, in which each of the fractions are resolved according to its isoelectric point.
MYPI2015702656A 2015-08-13 2015-08-13 A two-dimensional electrophoresis method for separation of protein compounds MY190479A (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
MYPI2015702656A MY190479A (en) 2015-08-13 2015-08-13 A two-dimensional electrophoresis method for separation of protein compounds
PCT/MY2016/050030 WO2017026890A1 (en) 2015-08-13 2016-05-06 A two-dimensional electrophoresis method for separation of protein compounds

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
MYPI2015702656A MY190479A (en) 2015-08-13 2015-08-13 A two-dimensional electrophoresis method for separation of protein compounds

Publications (1)

Publication Number Publication Date
MY190479A true MY190479A (en) 2022-04-22

Family

ID=57983959

Family Applications (1)

Application Number Title Priority Date Filing Date
MYPI2015702656A MY190479A (en) 2015-08-13 2015-08-13 A two-dimensional electrophoresis method for separation of protein compounds

Country Status (2)

Country Link
MY (1) MY190479A (en)
WO (1) WO2017026890A1 (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105527332B (en) * 2016-01-18 2018-05-22 云南省农业科学院花卉研究所 The extraction of lily total protein and the acquisition methods of dielectrophoresis differential protein collection of illustrative plates

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2008310248A1 (en) * 2007-10-09 2009-04-16 Dalhousie University Apparatus for purifying molecules

Also Published As

Publication number Publication date
WO2017026890A1 (en) 2017-02-16

Similar Documents

Publication Publication Date Title
MX2018013341A (en) Macromolecule analysis employing nucleic acid encoding.
WO2015195453A3 (en) Methods for increasing the capacity of flow-through processes
EA201792226A1 (en) POLYPEPTIDES CONTAINING THE DE NOVO BINDING DOMAIN AND THEIR APPLICATION
EA201891294A1 (en) THE METHOD OF WHICH A BISPECIFIC PROTEIN COMPLEX IS USED
EA201790247A1 (en) PLATFORM FOR CLEANING BISPECIFIC ANTIBODIES
EP4319162A3 (en) Method and apparatus for processing video signal
BR112017020102A2 (en) down sampling process for linear model prediction mode
MX2017006443A (en) Process of conducting high throughput testing high performance liquid chromatography.
EA201990321A1 (en) BISPECIFIC ANTI-CURRENT BINDING PROTEINS THAT SPECIFICALLY BIND TO CD3 AND CD123
EP3365045A4 (en) Multi-functional hemocompatible porous polymer bead sorbent for removing protein based toxins an potassium from biological fluids
EP2853895A3 (en) Methods, apparatus and systems for detection of total protein using capillary electrophoresis
PH12019500571A1 (en) Anti-pd-1 antibodies
MX2020000288A (en) Chromatography.
IL257146A (en) Method for the reduction of host cell proteins in affinity chromatography
SG10201807572PA (en) Robust antibody purification
NZ704076A (en) Electrophoretic separation of analytes
EP3523641A4 (en) Apparatuses, methods and systems for automated processing of nucleic acids and electrophoretic sample preparation
AR104358A1 (en) METHOD FOR PROTEIN PURIFICATION
WO2015106146A3 (en) Simultaneous extraction and separation of rna and dna from single cells using electrophoretic techniques
Nejadi et al. Comparison of three routine protein precipitation methods: acetone, TCA/acetone wash and TCA/acetone.
EP4300101A3 (en) Antigenic peptides and uses thereof for diagnosing and treating autism
MX2019004219A (en) Method for the detection of apolipoprotein e4.
MY190479A (en) A two-dimensional electrophoresis method for separation of protein compounds
EA201790725A1 (en) BASED ON THE APPROACH OF THE LOCATION OF THE METHOD OF CHOOSING PARTNERS FOR BINDING
MX2016016222A (en) System and methods of detecting and demonstrating ultraviolet damage to hair via evaluation of protein fragments.