MY190479A - A two-dimensional electrophoresis method for separation of protein compounds - Google Patents
A two-dimensional electrophoresis method for separation of protein compoundsInfo
- Publication number
- MY190479A MY190479A MYPI2015702656A MYPI2015702656A MY190479A MY 190479 A MY190479 A MY 190479A MY PI2015702656 A MYPI2015702656 A MY PI2015702656A MY PI2015702656 A MYPI2015702656 A MY PI2015702656A MY 190479 A MY190479 A MY 190479A
- Authority
- MY
- Malaysia
- Prior art keywords
- fractions
- separation
- eluted
- liquid phase
- dimension
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/24—Extraction; Separation; Purification by electrochemical means
- C07K1/26—Electrophoresis
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/24—Extraction; Separation; Purification by electrochemical means
- C07K1/26—Electrophoresis
- C07K1/28—Isoelectric focusing
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/416—Systems
- G01N27/447—Systems using electrophoresis
- G01N27/44756—Apparatus specially adapted therefor
- G01N27/44773—Multi-stage electrophoresis, e.g. two-dimensional electrophoresis
Landscapes
- Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Electrochemistry (AREA)
- Organic Chemistry (AREA)
- Biophysics (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Biochemistry (AREA)
- Analytical Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- General Chemical & Material Sciences (AREA)
- Peptides Or Proteins (AREA)
- Electrostatic Separation (AREA)
Abstract
The present invention relates to a method of separating protein compounds from a sample based on inverted two-dimensional polyacrylamide gel electrophoresis (i2D- PAGE) comprising the steps of: electrophoretically separating compounds from a sample loaded into a polyacrylamide gel tube in a first dimension into discrete fractions of different molecular weights, in which the separation is conducted using gel eluted liquid fraction entrapment electrophoresis whereby each of the fractions being eluted from the gel tube is trapped in a subsequent liquid phase; collecting the liquid phase containing each of the eluted fractions; and subjecting the liquid phase to a second separation in a second dimension using an isoelectric focusing medium in the presence of electric field, in which each of the fractions are resolved according to its isoelectric point.
Priority Applications (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
MYPI2015702656A MY190479A (en) | 2015-08-13 | 2015-08-13 | A two-dimensional electrophoresis method for separation of protein compounds |
PCT/MY2016/050030 WO2017026890A1 (en) | 2015-08-13 | 2016-05-06 | A two-dimensional electrophoresis method for separation of protein compounds |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
MYPI2015702656A MY190479A (en) | 2015-08-13 | 2015-08-13 | A two-dimensional electrophoresis method for separation of protein compounds |
Publications (1)
Publication Number | Publication Date |
---|---|
MY190479A true MY190479A (en) | 2022-04-22 |
Family
ID=57983959
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
MYPI2015702656A MY190479A (en) | 2015-08-13 | 2015-08-13 | A two-dimensional electrophoresis method for separation of protein compounds |
Country Status (2)
Country | Link |
---|---|
MY (1) | MY190479A (en) |
WO (1) | WO2017026890A1 (en) |
Families Citing this family (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN105527332B (en) * | 2016-01-18 | 2018-05-22 | 云南省农业科学院花卉研究所 | The extraction of lily total protein and the acquisition methods of dielectrophoresis differential protein collection of illustrative plates |
Family Cites Families (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2009046526A1 (en) * | 2007-10-09 | 2009-04-16 | Dalhousie University | Apparatus for purifying molecules |
-
2015
- 2015-08-13 MY MYPI2015702656A patent/MY190479A/en unknown
-
2016
- 2016-05-06 WO PCT/MY2016/050030 patent/WO2017026890A1/en active Application Filing
Also Published As
Publication number | Publication date |
---|---|
WO2017026890A1 (en) | 2017-02-16 |
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