MY185820A - Method for manufacturing culture product liquid - Google Patents
Method for manufacturing culture product liquidInfo
- Publication number
- MY185820A MY185820A MYPI2018702704A MYPI2018702704A MY185820A MY 185820 A MY185820 A MY 185820A MY PI2018702704 A MYPI2018702704 A MY PI2018702704A MY PI2018702704 A MYPI2018702704 A MY PI2018702704A MY 185820 A MY185820 A MY 185820A
- Authority
- MY
- Malaysia
- Prior art keywords
- stem cells
- culture
- liquid
- product liquid
- culture container
- Prior art date
Links
Landscapes
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
[Problem] To provide a method for manufacturing a culture product liquid, whereby a culture product liquid is manufactured which includes a predetermined metabolite secreted from a single type of stem cell. [Solution] The method for manufacturing a culture product liquid according to the present invention comprises: extracting an intermediate-layer bone marrow liquid positioned in an intermediate layer from bone marrow liquid separated in layered fashion and culturing the intermediate-layer bone marrow liquid together with a culture liquid; fixing first stem cells to a bottom surface of a first culture container and collecting the first stem cells from the first culture container when the total area of the first stem cells reaches a first target ratio of the bottom surface area of the first culture container; culturing second stem cells together with the culture liquid, the second stem cells being positioned in a bottom layer from among the first stem cells separated in layered fashion, while collecting the second stem cells; and fixing the second stem cells to the bottom surface of a second culture container, and extracting a culture product liquid including a predetermined metabolite secreted from a single type of second stem cells grown from the second culture container when the total area of the second stem cells reaches a second target ratio of the bottom-surface area of the second culture container. Fig. 1
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP2016047119 | 2016-03-10 | ||
JP2017044689 | 2017-03-09 | ||
PCT/JP2017/009546 WO2017155055A1 (en) | 2016-03-10 | 2017-03-09 | Method for manufacturing culture product liquid |
Publications (1)
Publication Number | Publication Date |
---|---|
MY185820A true MY185820A (en) | 2021-06-10 |
Family
ID=80933023
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
MYPI2018702704A MY185820A (en) | 2016-03-10 | 2017-03-09 | Method for manufacturing culture product liquid |
Country Status (1)
Country | Link |
---|---|
MY (1) | MY185820A (en) |
-
2017
- 2017-03-09 MY MYPI2018702704A patent/MY185820A/en unknown
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