MY166402A - Direct detection of unamplified hepatitis c virus rna using unmodified gold nanoparticles - Google Patents

Direct detection of unamplified hepatitis c virus rna using unmodified gold nanoparticles

Info

Publication number
MY166402A
MY166402A MYPI2013002624A MYPI2013002624A MY166402A MY 166402 A MY166402 A MY 166402A MY PI2013002624 A MYPI2013002624 A MY PI2013002624A MY PI2013002624 A MYPI2013002624 A MY PI2013002624A MY 166402 A MY166402 A MY 166402A
Authority
MY
Malaysia
Prior art keywords
hepatitis
virus
nanoparticles
unamplified
rna
Prior art date
Application number
MYPI2013002624A
Inventor
Abduo Sherif Mohamed Shawky
Azzazy El-Badawy Hassan Mohamed El-Said
Original Assignee
The American Univ In Cairo
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by The American Univ In Cairo filed Critical The American Univ In Cairo
Publication of MY166402A publication Critical patent/MY166402A/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
    • C12Q1/701—Specific hybridization probes
    • C12Q1/706—Specific hybridization probes for hepatitis
    • C12Q1/707—Specific hybridization probes for hepatitis non-A, non-B Hepatitis, excluding hepatitis D

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Communicable Diseases (AREA)
  • Immunology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Virology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

A gold nanoparticle-based colorimetric assay kit for hepatitis C virus RNA that detects unamplified HCV RNA in clinical specimens using unmodified AuNPs and oligotargeter polynucleotides that bind to HCV RNA. A method for detecting hepatitis C virus comprising contacting a sample suspected of containing hepatitis C virus with a polynucleotide that binds to hepatitis C virus RNA and with gold nanoparticles, detecting the aggregation of nanoparticles, and detecting hepatitis C virus in the sample when the nanoparticles aggregate (solution color becomes blue) in comparison with a control or a negative sample not containing the virus when nanoparticles do not aggregate (solution color remains red). (Fig.1)
MYPI2013002624A 2011-01-10 2011-01-10 Direct detection of unamplified hepatitis c virus rna using unmodified gold nanoparticles MY166402A (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PCT/US2011/020676 WO2012096646A1 (en) 2011-01-10 2011-01-10 Direct detection of unamplified hepatitis c virus rna using unmodified gold nanoparticles

Publications (1)

Publication Number Publication Date
MY166402A true MY166402A (en) 2018-06-25

Family

ID=46507350

Family Applications (1)

Application Number Title Priority Date Filing Date
MYPI2013002624A MY166402A (en) 2011-01-10 2011-01-10 Direct detection of unamplified hepatitis c virus rna using unmodified gold nanoparticles

Country Status (3)

Country Link
EP (1) EP2663649A4 (en)
MY (1) MY166402A (en)
WO (1) WO2012096646A1 (en)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP6217495B2 (en) 2014-03-31 2017-10-25 ブラザー工業株式会社 Piezoelectric actuator
CN113151584A (en) * 2021-01-11 2021-07-23 南通大学 SARS-CoV-2 detection kit and detection method
CN115961060A (en) * 2022-08-22 2023-04-14 中国海洋大学 Primer probe group, kit, method and application for isothermal amplification detection of mycobacterium tuberculosis based on nanogold

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
NL1002781C1 (en) * 1996-04-03 1997-10-06 Amsterdam Support Diagnostics Isolation and / or amplification of hepatitis C virus (HCV) nucleic acids from samples suspected of containing HCV.
US6083482A (en) * 1999-05-11 2000-07-04 Icn Pharmaceuticals, Inc. Conformationally locked nucleosides and oligonucleotides
WO2005067643A2 (en) * 2004-01-07 2005-07-28 Third Wave Technologies, Inc. Determination of hepatitis c virus genotype
WO2009131661A2 (en) * 2008-04-21 2009-10-29 Dicerna Pharmaceuticals, Inc. Methods and compositions for the specific inhibition of hepatitis c virus (hcv) by double-stranded rna

Also Published As

Publication number Publication date
WO2012096646A9 (en) 2012-08-30
EP2663649A4 (en) 2014-08-27
WO2012096646A1 (en) 2012-07-19
EP2663649A1 (en) 2013-11-20

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