MY141881A - Method and kit for detecting methicillin-resistant staphylococcus aureus - Google Patents

Method and kit for detecting methicillin-resistant staphylococcus aureus

Info

Publication number
MY141881A
MY141881A MYPI20052964A MY141881A MY 141881 A MY141881 A MY 141881A MY PI20052964 A MYPI20052964 A MY PI20052964A MY 141881 A MY141881 A MY 141881A
Authority
MY
Malaysia
Prior art keywords
gene
primers
resistant staphylococcus
staphylococci
staphylococcus aureus
Prior art date
Application number
Inventor
Professor Dr Shamala Devi Sekaran
Negar Shafiei Sabet
Professor Dr Parasakthi Navaratnam
Geetha Dr Subramaniam
Original Assignee
Univ Malaya
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Malaya filed Critical Univ Malaya
Priority to MYPI20052964 priority Critical patent/MY141881A/en
Publication of MY141881A publication Critical patent/MY141881A/en

Links

Abstract

THE PRESENT INVENTION PROVIDES AN ACCURATE AND PROMPT DETECTION OF METHICILLIN RESISTANT STAPHYLOCOCCUS AUREUS (MRSA) WHICH IS IMPORTANT FOR INFECTION CONTROL BY USING OLIGONUCLEOTIDES AS PRIMERS AND GENE-SPECIFIC LABELED PROBES IN PCR FOR THE SIMULTANEOUS AMPLIFICATION AND DETECTION OF GENES CARRIED BY METHICILLIN RESISTANT STAPHYLOCOCCUS AUREUS (MRSA). IN ORDER TO DETECT MRSA BY THE PCR METHOD IN DISTINCTION FROM OTHER STAPHYLOCOCCI, TWO KINDS OF PRIMERS ARE PROVIDED FOR AMPLIFYING SA-442 GENE EXPRESSING SPECIFICITY TO S. AUREUS SPECIES, TWO KINDS OF PRIMERS ARE PROVIDED FOR AMPLIFYING MECA GENE EXPRESSING THE METHICILLIN RESISTANCE AND TWO KINDS OF PRIMERS ARE PROVIDED FOR AMPLIFYING COA GENE EXPRESSED BY THE COAGULASE ENZYME PRODUCING STAPHYLOCOCCI FOR DISCRIMINATING S. AUREUS FROM THE OTHER STAPHYLOCOCCI, ARE COMBINED WITH THREE GENE-SPECIFIC PROBES TO THE TARGETED AMPLIFIED NUCLEIC ACID SEQUENCES FOR SPECIFIC DETECTION OF THE PRESENCE AND/OR ABSENCE OF THE AMPLIFIED GENE SEQUENCES, WHEREIN THE PRESENCE AND DETECTION OF ALL THREE AMPLIFICATION PRODUCTS ARE INDICATIVE OF METHICILLIN RESISTANT STAPHYLOCOCCUS AUREUS (MRSA) AND THE ABSENCE OF SA-442 AND/OR COA AMPLIFIED PRODUCT(S) IS INDICATIVE OF OTHER STAPHYLOCOCCI.
MYPI20052964 2005-06-29 2005-06-29 Method and kit for detecting methicillin-resistant staphylococcus aureus MY141881A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
MYPI20052964 MY141881A (en) 2005-06-29 2005-06-29 Method and kit for detecting methicillin-resistant staphylococcus aureus

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
MYPI20052964 MY141881A (en) 2005-06-29 2005-06-29 Method and kit for detecting methicillin-resistant staphylococcus aureus

Publications (1)

Publication Number Publication Date
MY141881A true MY141881A (en) 2010-07-16

Family

ID=44256679

Family Applications (1)

Application Number Title Priority Date Filing Date
MYPI20052964 MY141881A (en) 2005-06-29 2005-06-29 Method and kit for detecting methicillin-resistant staphylococcus aureus

Country Status (1)

Country Link
MY (1) MY141881A (en)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9777335B2 (en) 2001-06-04 2017-10-03 Geneohm Sciences Canada Inc. Method for the detection and identification of methicillin-resistant Staphylococcus aureus
US11834720B2 (en) 2005-10-11 2023-12-05 Geneohm Sciences, Inc. Sequences for detection and identification of methicillin-resistant Staphylococcus aureus (MRSA) of MREJ types xi to xx

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9777335B2 (en) 2001-06-04 2017-10-03 Geneohm Sciences Canada Inc. Method for the detection and identification of methicillin-resistant Staphylococcus aureus
US10577664B2 (en) 2001-06-04 2020-03-03 Geneohm Sciences Canada, Inc. Method for the detection and identification of methicillin-resistant Staphylococcus aureus
US10801074B2 (en) 2001-06-04 2020-10-13 Geneohm Sciences Canada, Inc. Method for the detection and identification of methicillin-resistant Staphylococcus aureus
US11834720B2 (en) 2005-10-11 2023-12-05 Geneohm Sciences, Inc. Sequences for detection and identification of methicillin-resistant Staphylococcus aureus (MRSA) of MREJ types xi to xx

Similar Documents

Publication Publication Date Title
Guk et al. A facile, rapid and sensitive detection of MRSA using a CRISPR-mediated DNA FISH method, antibody-like dCas9/sgRNA complex
JP7272744B2 (en) Sequences for the detection and identification of MREJ type XXI methicillin-resistant Staphylococcus aureus (MRSA)
WO2008122002A3 (en) Organism identification panel
Wang et al. Rapid detection of Staphylococcus aureus by loop-mediated isothermal amplification
Beaume et al. Orientation and expression of methicillin-resistant Staphylococcus aureus small RNAs by direct multiplexed measurements using the nCounter of NanoString technology
GB2445529A (en) Method for isothermal amplification of nucleic acids and method for detecting nucleic acids using simultaneous isothermal amplification of nucleic acids
RU2015122794A (en) REACTIONAL MIXTURES FOR PCR AND METHODS OF APPLICATION
MX2022014399A (en) Crispr-based assay for detecting pathogens in samples.
WO2019107893A3 (en) Method for amplifying target nucleic acid and composition for amplifying target nucleic acid
US20160047826A1 (en) Compositions and methods for detection of a target in a molecular assay using ph changes
EP2392668A3 (en) Control gene for normalising gene expression analysis data
WO2017160779A3 (en) Methods and kits to identify klebsiella strains
EP2589667A4 (en) Highly sensitive method for detecting mutated gene
CN105567866A (en) Primers, kit and method for detecting methicillin-resistant Staphalococcus aureus
WO2008108843A3 (en) Methods and kits for amplifying dna
WO2010151566A8 (en) Compositions and methods for detecting nucleic acid from mollicutes
MY141881A (en) Method and kit for detecting methicillin-resistant staphylococcus aureus
CN110546279A (en) detection and Classification of microorganisms Using ILV3 Gene
EP1862558A4 (en) Gene detection method
CA3156337A1 (en) Method for detecting target nucleic acid, method for detecting nucleic acid-binding molecule, and method for evaluating nucleic acid-binding ability
Pierce et al. Rapid detection of TEM-type extended-spectrum β-lactamase (ESBL) mutations using lights-on/lights-off probes with single-stranded DNA amplification
Gadkar et al. Evaluation of loop-mediated isothermal amplification (LAMP) to rapidly detect arbuscular mycorrhizal fungi
DE602006008263D1 (en) Method for the detection of Staphylococcus epidermidis
KR101881977B1 (en) Single nucleotide polymorphism marker for discriminating Ever-bearing or June-bearing strawberry cultivar and uses thereof
CN112029839A (en) Biosensor for rapidly detecting multiple drug-resistant acinetobacter baumannii by combining multiple PCR and CRISPR-Cas