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Application filed by Univ Malaysia TechfiledCriticalUniv Malaysia Tech
Priority to MYPI9701436priorityCriticalpatent/MY131798A/en
Publication of MY131798ApublicationCriticalpatent/MY131798A/en
Apparatus Associated With Microorganisms And Enzymes
(AREA)
Abstract
THE PRESENT INVENTION DESCRIBES A METHOD AND APPARATUS THAT MAY BE USED FOR HIGH DENSITY PERFUSION CULTURING OF MAMMALIAN OR INSECT CELLS. THE APPARATUS OF THE INVENTION USES A ROTARY FILTER (18) EXTERNAL TO THE REACTION VESSEL (14). THE ROTARY FILTER (18) HAS ITS DIMENSIONS AND SPEED OF ROTATION SO THAT THE CENTRIFUGAL FORCES AND TAYLOR VORTICES GENERATED IN OPERATION ARE SUCH AS TO AVOID FOULING OF THE FILTER ELEMENT (50) WHILE NOT BEING SUFFICIENTLY HIGH TO CAUSE SHEAR DAMAGE TO CELLS.IN A PREFERRED EMBODIMENT, THE PORE SIZE OF THE FILTERING ELEMENT (50) IS SUCH SO AS TO ALLOW PASSAGE OF DEAD CELLS WITH THE METABOLITE PRODUCT WHILE FILTERING OUT LIVE CELLS WHICH REMAIN IN THE RETENTATE AND ARE RECIRCULATED INTO THE REACTION VESSEL (14).(FIG 1)
MYPI97014361997-04-031997-04-03External filtration method and apparatus for perfusion cell culturing
MY131798A
(en)