Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US06/338,397external-prioritypatent/US4551433A/en
Priority claimed from EP19820302532external-prioritypatent/EP0067540B1/en
Application filed by Genentech IncfiledCriticalGenentech Inc
Publication of MY100360ApublicationCriticalpatent/MY100360A/en
Preparation Of Compounds By Using Micro-Organisms
(AREA)
Abstract
HYBRID PROMOTER/OPERATORS ARE PREPARED AND INSERTED INTO PLASMIDS WHICH ARE USED TO TRANSFORM E. COLI. THEY ARE ARRANGED TO CONTROL EXPRESSION (BY A GENE IN THE PLASMID) OF A DESIRED HETEROLOGOUS PEPTIDE E.G. HUMAN GROWTH HORMONE, HGH. EACH HYBRID PROMOTOR/OPERATOR HAS (A) A PROMOTER/OPERATOR FRAGMENT WHICH IS CAPABLE OF BEING CHEMICALLY INDUCED, AND (B) A FRAGMENT WHICH INCLUDES THE -35 CONSENSUS SEQUENCE AND THE 5'' FLANKING REGION THEREOF OF A PROMOTER HAVING A HIGH SIGNAL STRENGTH. THE FRAGMENTS (A) AND (B) ARE APPROPRIATELY SPACED TO FORM AN OPERABLE HYBRID PROMOTER WHICH IS BOTH INDUCIBLE AND OF HIGH SIGNAL STRENGTH (PROPERTIES NOT NATURALLY FOUND TOGETHER). E.G. ONE HYBRID OPERATOR/PROMOTER HAS A SEQUENCE FROM UPSTREAM OF POSITION -22 OF THE TRP PROMOTER (OF HIGH STRENGTH) COUPLED TO A SEQUENCE DOWNSTREAM FROM POSITION -19 OF THE LAC PROMOTER/OPERATOR (INDUCIBLE). THIS WAS USED TO CONTROL A GENE FOR HGH IN A PLASMID PHGH807TACI. E. COLI WAS TRANSFORMED THEREWITH AND CULTURED. PRODUCTION OF HGH (RAPID OWING TO THE STRONG PROMOTER FRAGMENT) WAS INDUCED BY ADDITION OF ISOPROPYL-(BETA)-D-GALACTOSIDE TO THE CULTURE MEDIUM.
Method for polypeptide production involving expression of a heterologous gene, recombinant microbial cloning vehicle containing said gene and bacterial culture transformed by said cloning vehicle
Cloned human basic fibroblast growth factor, methods for its preparation, compositions containing it, dna sequences encoding therefor and vectors and host cells containing said dna
A xenogeneic expression control system, a method of using it, expression vectors containing it, cells transformed thereby and heterologous proteins produced therefrom.
RECOMBINANT PLASMIDE, BACTERIA CONTAINING THIS RECOMBINANT PLASMIDE, METHOD FOR PRODUCING FERMENTED FOODSTUFFS, ANIMAL FEED, COMPONENTS OF THE SAME OR PROTEINS USING THESE BACTERIA.