MXPA99011404A - Treatment of fabrics, garments, or yarns with haloperoxidase - Google Patents

Abstract

A new method of treating undyed fabrics, garments, or yarn comprising treating the undyed fabric, garment, or yarn in an aqueous medium with a haloperoxidase, a halide source and a hydrogen peroxide source.

Description

TREATMENT OF FABRICS, CLOTHES, OR YARNS WITH HALOPEROXIDASE FIELD OF THE INVENTION The present invention relates to a new method for the treatment of non-dyed fabrics, garments, or yarns, where the method comprises the treatment of the fabric. , garment or yarn not dyed, in an aqueous medium, with a haloperoxidase, a source of halide and a source of hydrogen peroxide.

BACKGROUND OF THE INVENTION Fabrics composed of materials such as wool and, in particular, cellulose compounds such as cotton, are often discolored during manufacturing operations. Hydrogen peroxide is often used as a bleaching agent. In addition to hydrogen peroxide, decolorizing solutions will normally contain silicas, caustic agents, chelants, cyanic stabilizers, magnesium salts, and rurecta? Ss. EL trataraaito ds fcOa μ = o ti = re dbs primary faracpes: the first is dLau. nor high level of detail; and the join (when the fabric is a cellulosic material) is to break and solubilize the materials that form the specks. Typical bleaching conditions are 1.5-1.5% hydrogen peroxide; 0.5-2% sodium silicate; 0.1-0.4% caustic compound; and 0.2% chelating agents, at a temperature of 100 ° C. WO "* - REF .: 31894 92/18683 discloses a process for bleaching fabrics dyed with peroxidases and oxidases.In addition, goods, clothing, or yarns are sometimes treated with the purpose of improve the characteristics of dyeing (coloring), such as dye uptake In addition, the genera, garments or yarns of wool or other hair fibers of animals are sometimes treated in order to protect them against the tendency The methods used to produce shrink-resistant fabrics, garments or yarns are known.The method most commonly used for wool is the Hercosett IWS / CSI O Chlorine process, which comprises an acid chlorination of wool, followed by An application of a polymeric compound, this process imparts a high degree of resistance to wool shrinkage, but it adversely affects the handling of wool and generates waste that damages the environment. I write other methods to reduce e! shrinkage of goods, clothing, or yarns, which do not result in the release of substances that harm the environment, including processes such as plasma treatments at low temperatures.

BRIEF DESCRIPTION OF THE INVENTION The purpose of the present invention is to provide a method, based on enzyme, for the treatment of fabrics, garments, or yarns, in order to provide advantages with respect to an improved bleaching effect, dye uptake, and / or shrinkage resistance, and by means of which it is possible to reduce the damage to the fiber and limit the use of chemical compounds that harm the environment.Now it has been found that certain properties of the goods, garments of clothing, or yarns, may be improved by subjecting the non-dyed cloth, garment, or yarn to a treatment with I or peroxidase together with a source of hydrogen peroxide and a halide source, in an amount effective to provide the desired effect One embodiment of the invention provides a method for the manufacture of a bleached, garment, or yarn, comprising the fabric, the garment, or the garment. ila d * o, unstained, in an aqueous medium, with an effective amount of a haloperoxidase, a halide source and a peroxide source, at a lower temperature, typically 30 to 70 ° C, than the temperature used in a traditional bleaching process with hydrogen peroxide. This modality provides a process for bleaching the fabric, garment, or yarn, not dyed, at a temperature below 100 ° C, and a bleaching process that requires fewer chemical compounds than what is currently required. Another modality provides a method for bleaching motes in a genus, garment, or spinning, cellulosic, in an aqueous medium, with an effective amount of a haloperoxidase, a source of halide and a source of hydrogen peroxide. Another embodiment of the invention provides a method for manufacturing goods, garments, or yarns, with improved shrinkage resistance and dye uptake. The fabric, garment, or yarn is preferably wool.

Other aspects of the invention will become apparent from the following detailed description and claims.

DETAILED DESCRIPTION OF THE INVENTION Before describing the methods of the invention, it should be understood that this invention is not limited to the methods described. The terminology used herein is for the purposes of describing particular embodiments only, and is not intended to be limiting, because the scope of the present invention will be limited only by the appended claims. As used in the specification and in the appended claims, the singular fora "a", "an" and "the", "the", include plural references, unless the context clearly indicates otherwise. Accordingly, for example, references to "haloperoxidase" or "haloperoxidase preparation" include mixtures of this haloperoxidase; the reference to "the method" includes one or more methods and / or steps "of the type described herein and / or which will become apparent to all those trained in the art, after reading this description, and so on. Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by any of the ordinary art-trained persons to which the invention pertains, although any methods and materials may be used. Similar or equivalent to those described herein, in the practice or evaluation of the present invention, preferred methods and materials are now described.All publications referred to herein are incorporated by reference for the purpose of explaining and describing the connection material for which the reference was cited The term "non-dyed" refers to the gender, garment, or yarn, which has not been completed. a process of dyeing (coloring). The dyeing (coloring) can be carried out, optionally, during or after the method according to the invention. Preferably, the enzymatic treatment is carried out before the stage of having (coloring). The term "bleaching" is defined herein as a bleaching of the fabric, garment, or yarn, and can be measured using the change in the spatial coordinates of the color, L * a * b * (CIELAB system): L * gives the change in the white / black ratio on a scale from 0 to 100. A decrease in L * means an increase in black color (decrease in white color); an increase in L * means an increase in white color (a decrease in black color). Bleaching can also be measured using the Stensby units (W = L + 3a-3). The fabric can be constructed from fibers by weaving, knitting or non-woven operations. Weaving and knitting require yarn to be the input, while a non-woven fabric is the result of the random union of fibers (paper can be thought of as an article not left). The woven fabric is constructed by weaving "non-fibrous" or weft yarns between coated yarns stretched in the longitudinal direction of the loom. Coated yarns must be prepared before they are woven, in order to lubricate them and protect them from abrasion in the high-speed insertion of the weft yarns during weaving. The weft yarn can be woven through the coated yarns in a "on one - under the next" ("over one -under the next") (flat drawing) or in the "over one-under two" mode. ("over one - uñder two") (cross weaving) or any other myriad of permutations. The resistance to tension, texture and pattern are related not only to the type / quality of the yarn, but also to the type of pattern. In general, dresses, shirts, pants, sheets, towels, curtains, etc., are produced from woven fabrics.

The knitted fabric forms a fabric by joining interlaced yarn loops. Contrary to weaving, which is constructed from "two types of yarn and which has many" ends ", the knitwear is produced from a single continuous braid of yarn, or it happens with the weaving, there are ~~ m Many different ways of interlacing yarn and the final properties of the yarn depend on both the yarn and the type of braid, underwear, sweaters, anklets, T-shirts, sweatshirts, etc., are derived from woven fabrics. The non-woven fabrics are sheets of fabric that are made by joining and / or interlacing fibers and filaments by means of mechanical, thermal, chemical or solvent-mediated processes.The resulting fabric can be found in the form of cross-linked structures, in the form of film or films, typical examples are disposable diapers for babies, towels, cloths, surgical gowns, garments for "environmentally friendly" fashion, filter media, effects to bed, roofing materials, bases for two-dimensional fabrics and many others. According to the invention, the process can be applied to any type known in the art (knitting, knitting, or non-knitting). In particular, the bleaching process can be applied to cellulose-containing genera or to cellulosic genera, such as cotton, viscose (cellulose xanthate), rayon, ramie, li, or ocel (eg, Tencel, produced by Courtaulds Fibers), or mixtures of any of these fibers, together with synthetic fibers (eg, polyester, polyamide, nylon) or other neutral fibers such as wool and silk. The term "wool" includes any animal hair product that is useful co-rrily, for example, wool of sheep, camel, rabbit and goat or llama, and includes wool fiber and animal hair. The method of the invention can be used with wool or animal hair material in the form of combed ribbon, fiber, yarn, or woven or knitted fabric. The enzymatic treatment may also be carried out on loose lint or on garments made of wool material or animal hair. The treatment can be carried out in many different stages of processing. The term "shrinkage" refers to the felting shrinkage of the fissures as defined in IWS TM 31, ie, the shrinkage by felting is the irreversible shrinkage caused by the progressive entanglement of wool fibers induced by washing in aqueous solution. , and is defined as the reduction in length and / or width induced by washing. The shrinkage can be measured in accordance with the IWS TM 31, or it can be measured using the following modification. The samples of wool (24 cm X 24 cm) are sewn around the borders and are inscribed in a rectangle (18 cm X 18 cm). The samples are treated, dried with air, then subjected to five cycles of washing machine and then dried (hot wash, high heat drying), in combination with external ballast such as towels and clothing items. The dimensions of the rectangle are measured after five cycles, and shrinkage is defined as the change in the dimensions of the rectangle, after taking into account initial shrinkage shrinkage. The term "shrinkage resistance" is a measure of the reduction in shrinkage (as defined above, after the drying / drying cycles) for the material that has been treated, coli with respect to the material that has not been treated. ., ie, Shrinkage resistance = (shrinkage-treat ± shrinkage ^^^^) / shrinkage frt ^ t? i The value is multiplied by 100 in order to be expressed as a percentage. The term "dye uptake" refers to the properties associated with the coloring of fabrics, garments or yarn, or wool or animal hair material. The dye uptake is a measure of the capacity of wool material or animal hair, immersed in a dye solution (dye), to absorb the available coloring material. This propi e ^ Jad can be measured by the following test. In a suitable reaction vessel, wool or animal hair material is added to a buffered solution of acid black 172 (300 mL of 0.05 M NaOAc buffer, pH 4.5, plus 7.5 mL of a 10% solution). / p, of acid black 172 in water). The vessel is incubated in a shaking water bath, at 50 ° C, for 15 minutes, with gentle agitation. After removing the material from the solution, it is allowed to air dry, then it is measured in a suitable spectrophotometer to determine the CIELAB values. The dye uptake (dye) is determined by the L * reciura, and changes in dye uptake are found by determining dL *, with respect to the untreated material. The "speckled" particles are dark brown particles found in non-discolored cotton genera, also called "black dots". They are residues are residues of capsules and cotton stalks that originate from the mechanical harvesting of cotton. The brown color is due to the high lignin content of the speck particles.

Haphoperoxidases In the context of the present invention, the term "haloperoxidase" means an enzyme that is selected from the group consisting of the chloride peroxidase (EC 1.11.1.10), bromide peroxidase, and iodide peroxidase (EC 1.11.1.8). A peroxidase chloride is an enzyme capable of oxidizing the chloride, bromide and iodide ions, with the consumption of H202. A bromide peroxidase is an enzyme capable of oxidizing bromide and iodide ions with the consumption of H202.

An iodide peroxidase is an enzyme capable of oxidizing iodide ions with the consumption of H202. Haloperoxidases form a class of enzymes capable of oxidizing halides (X = CI ', Bd, or I ") in the presence of hydrogen peroxide, up to the corresponding hypohalose acid (HOX), according to the equation: H202 + X" + H +? H20 + HOX If a suitable nucleophilic compound is present, a reaction with the HOX will occur, whereby bleaching may occur. Haloperoxidases have been isolated from various organisms: mammals, marine animals, plants, algae, lichen, fungi and bacteria (for reference, see Biochimica et Biophysica Acta 1161, 1993, pp. 249-256). It is generally accepted that haloperoxidases are the enzymes responsible for the formation of halogenated compounds in nature, although other enzymes may also be involved. Haloperoxidases have been isolated from many different fungi, in particular, from a group of fungal dematiaceous hyphomycetes, such as Ca dariomyces, e.g., C. fu magician.

Alternaria. Cu rvu la ría, e.g., C. verruculosa and C. inaequalis. Drechslera Ulocladiu m and B o t r y t i s (see U.S. Patent No. 4,937,192). In accordance with the present invention, a haloperoxidase which is obtained from Cu rvularia, in particular from C. verruculosa, is preferred. Curvularia haloperoxidase and recombinant production thereof is described in WO 97/04102. "Haloperoxidase has also been isolated from bacteria such as Pseudomonas, eg, P. pyrrocinia (for reference, see The Journal of Bioloaical Chemistry 263, 1988, pp. 13725- 13732) and Streptomyces, eg, S. aureofaciens (for reference see Structural Bioloay 1_, pp. 532-537) Bromide peroxidase has been isolated from algae (see US Patent No. 4,937,192) During use, the concentration of the haloperoxidase may vary in order to achieve the desired effect of bleaching. In the desired time scheme, however, according to the invention, the haloperoxidase will normally be added at a concentration of 0.01-100 mg of enzyme protein per liter, preferably at a concentration of 0.1-50 mg of enzyme protein per liter. more preferably, in a concentration of 1-10 mg of enzyme protein per liter.

Halide Sources According to the invention, the halide source for the reaction with haloperoxidase can be achieved in many different ways: the halide source can be sodium chloride, "potassium chloride, sodium bromide, potassium bromide, iodide of sodium or potassium iodide The concentration of the halide source will typically correspond to 0.01-1000 mM, preferably in the range of 0.1-500 mM.

Sources of Hydrogen Peroxide According to the invention, the hydrogen peroxide necessary for the reaction with I to haloperoxidase, can be established in many different ways: it can be hydrogen peroxide or a precursor of hydrogen peroxide, such as percarbonate or hydrogen peroxide. perborate; or a percarboxylic acid, or a salt of the ism; or it can be an enzymatic system that generates hydrogen peroxide, such as an oxidase and its substrate. Useful oxidases include glucose oxidase, a glycerol oxidase or an amino acid oxidase. An example of an amino acid oxidase is given in WO 94/25574. According to the invention, the source of hydrogen peroxide necessary for the reaction with the haloperoxidase can be added in a concentration in the range from 0.01-1000 mM, preferably, in the range from 0. 1-500 mM.

Process The chosen procedure will depend on the haloperoxidase in question, taking into account the optimum pH, the optimum temperature, etc. If a haloperoxidase obtained from Curvularia verrucosa is used, the processing conditions could be: 30-70 ° C, pH 5, using 1-5 mg enzyme / liter, 50-500 mM halide (eg sodium chloride) ), 20 M hydrogen peroxide, at a liquor / gender ratio from 4: 1-30, for a reaction time of 30-120 minutes (as illustrated in Example 1). A buffer * can be added to the reaction medium to maintain a pH suitable for the haloperoxidase used. The buffer may suitably be a phosphate, borate, citrate, acetate, adipate, triethanolamine, monoethanolamine, diethanolamine, carbonate (especially alkali metal or alkaline earth metal, in particular, sodium or potassium carbonate, or ammonium salts and of HCt), diamine, especially dimaminoethane, imidazole, or an amino acid buffer. The process of the invention can be carried out in the presence of a conventional fabric, garment, or yarn finishing agents, including wetting agents, polymeric agents, dispersing agents, etc. A conventional wetting agent can be used to improve contact between the substrate and the enzyme used in the process. The wetting agent can be a non-ionic surfactant, e.g., an ethoxylated fatty alcohol. A very useful wetting agent is an ethoxylated and propoxy fatty acid ester., such as Berol 087 (product of Akzo Nobel, Switzerland). Suitable nuclei of suitable polymers include proteins (eg, bovine serum albumin, milk proteins, milk, casein or legume proteins), protein hydrolysates (eg, hydrolysates of whey proteins, casein or protein from soybean), polypeptides, lignosuiponates, polysaccharides and derivatives thereof, polyethylene glycol, polypropylene glycol, polyvinyl pyrrolidone, ethylene diamine condensed with ethylene or propylene oxide, ethoxylated polyamines, or ethoxylated amine polymers. The dispersing agent can be selected, suitably, from nonionic, anionic, cationic, ampholytic or amphoteric surfactants. More specifically, the dispersing agent can be selected from carboxymethylcellulose, hydroxypropyl cellulose, alkyl aryl sulphonates, long-chain alcohol sulfates (primary and secondary alkyl sulfates), sulphonated olefins, sulphated monoglycerides, sulfated ethers , sulfosuccinates, sulphonated methyl ethers, alkane sulfonates, phosphate esters, alkyl isothiolates, acyl sarcosides, alkyl taurides, fluororesurfactants, fatty alcohol condensates and alkylphenol, fatty acid condensates, condensates of ethylene oxide with an amine, condensates of ethylene oxide with an amide, sucrose esters, sorbitan esters, alkylamides, fatty amine oxides, ethoxylated monoamines, ethoxylated diamines, alcohol ethoxylate, and mixtures thereof. A very useful dispersing agent is an alcohol ethoxylate, such as Berol 08 (product of Akzo Nobel, Switzerland). The bleaching process can be carried out in any machinery known in the art.

Inactivation of the haloperoxidase in question will not normally be necessary; however, if Inactivation of the enzyme is desired, this can be carried out as is known in the art, eg, elevated temperature and / or high pH, but the specific inactivation conditions will, of course, depend on the enzyme in use. . The fabric may be further finished by one or more of the following treatments, as is known in the art: dyeing, biological polishing, polishing, smoothing, and / or anti-wrinkling treatments.

Test Procedure The test procedure for the bleaching of the fabric can be carried out visually and by using a Chroma Meter CR200, from Minolta; a Chroma Meter CR300, from Minolta; or a Chroma Meter 508 i, from Minolta.

E v aluation: A Chroma Meter from Minolta (available from Minolta Corp.) is used, in accordance with the manufacturer's instructions, to evaluate the degree of bleaching, as well as to estimate any discoloration using the change in the spatial coordinates of the color L * a * b * (CIELAB system): L * gives the change in the white / black value, on a scale from 0 to 100; a * gives the change in green value (-a *) / red (+ a *); and b * gives the change in the blue value (-b *) / yellow (+ b *). A reduction in L * means an increase in black color (decrease in white color), an increase in L * means an increase in white color (a decrease in black color); a decrease in a * means an increase in green color (decrease in red color), an increase in a * means an increase in red color (a decrease in green color); a decrease in b * means an increase in the blue color (decrease in the yellow color), and an increase in b * means an increase in the yellow color (a decrease in the blue color). The instrument is calibrated using a standard gauge plate (white). The invention is further illustrated in the following examples, which are not intended to be limited, in any way, to the scope of the invention, as claimed.

EXAMPLE 1 Bleaching of cuttings from samples of raw cotton with the verrucous Curve-haloxidase conditions Experimental conditions: The bleaching system contained 3 mg / L of the recombinant haloperoxidase of C? Rvularia verruculosa with [NaCl] = 100 mM as substrate, and [H2? 2] =: 0 mM, as donor.

The pH was adjusted to pH = 5. The sample cuttings were bleached for 60 minutes at 40 ° C. (The enzyme was produced as described in WO 97/04102).

The bleaching system was evaluated on cuts of cross-woven cotton samples and cuts of woven cotton samples. For the cross-woven genera, the gender / liquor ratio was: 1 g of gender in 15 mL of aqueous medium. For the woven fabric, the gender / liquor ratio was: 1 g of gender in 20 mL of aqueous water.

Results: Significant visual bleaching was obtained with the experimental conditions described above. Note that the blind test ensures that the bleaching effect is enzymatic in nature. The bleaching results are presented in the following Table 1: TABLE i:? L * /? A * /? B * in cuts of raw cotton samples3. Crossed fabric system1 Bleaching Fabric1 Ciegoc (-) 0.2 / 0.1 / 0.0 (-) 0.2 / 0.0 / (-) 0.1 Enzyme "2.5 / (-) 0.9 / (-) 1.5 1.6 / (-) 0.6 / (-) 1.3 a: All measurements were made on a Minolta 508 i device. The lamp was adjusted to D65 and 2 °. b: The cuttings of the unsized samples were obtained from the Test Genres. c: The system consists of NaCl, hydrogen peroxide and acetates buffer. °: The system consists of haloperoxidase, NaCl, hydrogen peroxide and acetates buffer.

EXAMPLE 2 Bleaching of the specks with the Haloperoxidase of Curvularia verrucosa. Experimental conditions: The bleaching system was the same as that described in the mp 1: 3 mg / L axis of the recombinant haloperoxidase of Curvularia verrucosa with [NaCl] ] = 100 mM, as substrate; and [H2O2] = 20 mM as donor. The pH was adjusted to pH = 5. The samples were bleached for 60 minutes at 40 ° C in an LP2 Lauder-o-meter from Atlas. The 100% cotton woven linen was provided by Nordisk Textil Vaeveri & Trykkeri A / S. The gender / liquor ratio was 1 g of gender in 20 mL of aqueous medium.

Results: The specks were counted in an area of the genus of 10 cm x 15 cm (left over both sides). A speck was defined as a "dark spot" on the cotton surface, regardless of size. The double determination of the bleaching effect of the specks was carried out.

Numbers 1 and 2, in Table 2, refer to the separate cloths of the genus that were used. Note that a positive difference in the count of specks can be due to the division of the specks due to the mechanical handling of the cloth.

Table 2: Count of Difference Counts in speckles before speckles after counting the whitening bleaching speckles Side of cloth of gender Side 1 Side 2 Side 1 Side 2 Side 1 Side 2 Reference 1 B 85 74 91 71 +6 -3 Refere nce 2a 78 68 69 70 -9 +2 Blind lb 60 _ 50 62 52 +2 +2 Blind 2b 72 74 77 75 +5 +1 Enzyme tico lc 53 62 49 42 -4 -20 Enzymatic 2C 68 62 41 56 -27 -6 a: Gender washed only in shock absorber. b: Conditions as described above, in the experimental section, but without added enzyme. c: Conditions as described above in the experimental section.

The reference tests illustrate the effects of the mechanical washing procedure, as can be seen from Table 2, the loss of the specks is ambiguous. (The mechanical washing procedure has no significant effect on the number of spots remaining on the cloth after bleaching).

Table 2 shows that there is a significant loss of the specks when the cloth of the sort is subjected to enzymatic bleaching conditions. The blind test ensures that the observed effect is enzymatic in nature.

EXAMPLE 3 Treatment of the wool with the haloperoxidase of Curvularia see rucu slab Experimental conditions: The enzyme system was the same as that described in Example 1: 3 mg / L of recombinant haloperoxidase of C? Rvularia verruc? Lasa, with [ NaC!] = 20 mM, as substrate; and [H2O2]! ß20 mM, as a donor. The pH was adjusted to pH = 5. Sample pieces (24 cm x 24 cm, approximately 10 g each) were cut from Jersey Jersey TF532, and sewn around the edge with a needle. A permanent marker was used to draw a rectangle of 18 x 18, in each piece of sample.

Results: Treatment Shrinkage (%) Blind 29 Epitimatic 23 It is noted that, in relation to this date, the best method known by the applicant to carry out the aforementioned invention is the conventional one for the manufacture of the objects or substances to which the it refers.

Claims (23)

CLAIMS Having described the invention as above, the content of the following claims is claimed as property.

1. A method for the treatment of goods, garments, or yarns, characterized in that it comprises the treatment of the gender, garment or yarn, not dyed, in an aqueous medium, with an effective amount of a haloperoxidase, a source of halide and a source of hydrogen peroxide.

2. "The method, according to claim 1, characterized in that the fabric, the garment, or the yarn, is a cellulosic fabric.

3. The method, according to claim 1, characterized in that the fabric, the garment, or the yarn is a sort of silk or a wool fabric.

4. The method, according to claim 2, characterized in that the cellulosic type is mahon.

5. The method, according to claim 1, characterized in that the haloperoxidase is obtained from fungi, bacteria, or algae.

6. The method according to claim 5, characterized in that the haloperoxidase is obtained from a fungus that is selected from the group consisting of Caldariomyces. Alternaria. Curvularia, Drechslera, U loclad iu m and Botrvtis.

7. The method, according to claim 6, characterized in that the haloperoxidase is obtained from Curvularia.

8. The method, according to claim 7, characterized in that the haloperoxidase is obtained from Curvularia ver rucu slab.

9. The method, according to claim 5, characterized in that the haloperoxidase is obtained from a bacterium selected from the group consisting of Pseudomonas and Streptomvces.

10. The method, according to claim 1, characterized in that the concentration of the haloperoxidase is in the range of 0.01-100 mg of enzyme protein per liter.

11. The method according to claim 1, characterized in that the halide source is sodium chloride, potassium chloride, sodium bromide, potassium bromide, sodium iodide or potassium iodide.

12. The method, according to claim 1, characterized in that the source of hydrogen peroxide is hydrogen peroxide, or a precursor of hydrogen peroxide, e.g., percarbonate or perborate; or an epzymatic system that generates hydrogen peroxide, e.g., an o idasa and its substrate; or a percarboxylic acid, or a salt thereof.

13. The method, according to claim 11, characterized in that the concentration of the halide source corresponds to 0.01-1000 mM.

14. The method according to claim 12, characterized in that the concentration of the source of hydrogen peroxide corresponds to a concentration of the hydrogen peroxide in the range from 0.01-1000 mM.

15. The method, according to claim 1, characterized in that the haloperoxidase is applied at a temperature below 70 ° C.

16. The method, according to claim 1, characterized in that the aqueous medium contains a surfactant.

17. A method for manufacturing a bleached fabric, characterized in that it comprises the treatment of the non-dyed fabric by the method according to any of claims 1-16.

18. A method for manufacturing a bleached yarn, characterized in that it comprises the treatment of the non-dyed yarn by the method according to any of claims 1-16.

19. A method for bleaching motes in a genre, characterized in that it comprises treating the non-dyed fabric by the method according to any of claims 1-16.

20. The method, according to any of claims 17-19, characterized in that the fabric or yarn is made of cellulosic material.

21. A method for manufacturing goods, garments, or yarns, with improved resistance to shrinkage, wherein the method is characterized in that it is the method according to any of claims 1-16.

22. A method for manufacturing sorts, garments, or yarns with improved color pick-up, wherein the method is characterized in that it is the method according to any of claims 1-16.

23. The method, according to any of claims 21-22, characterized in that the fabric, the garment or the yarn are wool.