MXPA99009467A - Plants with controlled side-shoot formation and/or controlled abscission area formation - Google Patents

Abstract

Disclosed are nucleotide sequences coding polypeptides which are responsible for controlling side-shoot formation and/or petal formation and/or abscission area formation, in addition to polypeptide and amino acid sequences coded by nucleotide sequences. Disclosed are also plants with controlled side-shoot formation and/or petal formation and/or controlled formation of abscission areas, wherein the expressible DNA sequence, fragment or derivative thereof responsible for side-shoot formation and/or petal formation and/or abscission area formation is integrated in a stable manner into the genome of the plant cell or the plant tissue. Further disclosed are methods for the production of plants with controlled side-shoot formation and/or petal formation and/or controlled formation of abscission areas, wherein the expressible DNA sequence or fragment or derivative thereof responsible for side-shoot formation and/or petal formation and/or controlled formation of abscission areas is integrated in a stable manner into the genome of plant cells or plant tissue and the plant cells or plant tissue thus obtained is regenerated to form plants. The invention also relates to plants and the seeds of plants which can be obtained according to the inventive method.

Description

PLANTS WITH CONTROLLED TRAINING OF SIDE RETONES AND / OR CONTROLLED FORMATION OF ABSTRACT ZONES FIELD OF THE INVENTION The present invention relates to nucleotide sequences that encode polypeptides that are responsible for the control of side shoot formation and / or formation of petals and / or formation of abscission zones, as well as polypeptides and sequences of amino acids encoded by these nucleotide sequences. Additionally, the present invention relates to plants having controlled lateral sucker formation and / or controlled formation of petals and / or controlled formation of abscission zones, wherein the expressible DNA sequence or fragment or derivative thereof responsible for the formation of side shoots and / or formation of petals and / or formation of abscission zones is stably integrated into the genome of the cell or plant tissue. Additionally, the invention relates to methods for the production of plants having controlled lateral sucker formation and / or controlled formation of petals and / or controlled formation of abscission zones, wherein the expressible DNA sequence or fragment or derivative of the same responsible for the formation of side shoots and / or formation of petals and / or formation of zones of P1593 / 99MX abscission is stably integrated into the genome of plant cells or tissues and the resulting cells or plant tissues regenerate to form plants. Additionally, the invention relates to plants and seed varieties of plants, which can be obtained according to the method of the invention.

TECHNICAL BACKGROUND The performance characteristics of the economic and ornamental plants are determined considerably by their architecture. While the basic structure of a plant is manifested in embryonic development, the post-embryonic phase is characterized by the activity of apical meristems. Of fundamental importance is the ability of the shoot apical meristem (SAM) of higher plants to initiate shoots and control their development. As a result, the habit of a plant, and in this way, an essential characteristic of performance is characterized by the number, arrangement and intensity of development of its lateral shoots. Branching of shoots may occur terminally, as well as laterally. The terminal branch in which the SAM is separated into two portions occurs mainly in lower corraophytes and has been described only for a few flowering plants P1593 / 99MX (Steeves and Sussex, 1989, Patterns in Plant Development, 2nd Edition, Cambridge University Press, Cambridge). The typical lateral branch for flowering plants is based on the formation of new shoot apical eristems in the armpits of the leaves, which are derived from the SAM cells, the meristemic character of which remains conserved in contrast to the surrounding cells. they are included in the development of the primordia of the leaf. In the further course of development, a lateral outbreak of the residual meristems is formed, in addition to some primordia of the leaves containing an apical meristem, whose activity is subjected to the control by the SAM of the main shoot. The analysis of the plant mutants revealed that the branching of the shoot system is controlled by genetic factors. Thus, in the tomato (Lycopersi with escul entum) for example, several mutants have been described, the formation of lateral shoots of which is inhibited in different stages (for example, blind, blind-2, torosa, lateral suppressor) . A morphological characterization showed that the production of armpit shoots is altered in tomato mutants bl ind, bl ind-2 and torosa (Tucker, 1979, Ann. Bot. 43: 571-577; Mapelli and Lombardi, 1982, Plant &; Cell Physiol., 23: 751-757). In contrast, in plants that are homozygous for P1593 / 99MX recessive mutation of the teral suppressor (Is), initiation of most side shoots does not occur (Brown, 1955, Rep. Tomato Genetics Cooperative 5: 6-7). A histological analysis (Malayer and Guard, 1964, A er. Jour. Bot. 51: 140-143) shows that the cells derived directly from the SAM in the axils of the primordia of the leaf, in the meristemic activity on which it is based the formation of lateral shoots, being absent in the mutant of the teral suppressor. If a lack of lateral shoots in all axils of the leaves results in a termination of the shoot axis in the first flowering, the transition to floral development shows that the ability to establish axillary meristems is not completely lost in the mutant. In the armpit of the leaf primordium established directly before flowering, a meristem is frequently established in ls and homozygous mutants. The establishment of this meristem that is necessary for the sympodial structure of the shoot axis is often associated by the formation of a lateral shoot in the axilla of the next greater leaf. After the transition to the floral phase, the development of the Is mutant is characterized by a smaller number of flowers per bloom (Williams, 1960, Heredity, 14: 285-296), the missing establishment of the petal primordia (Szymkowiak and Sussex, 1993, Plant J., 4; 1-7) P1593 / 99MX and an abnormal number of stamens and carpels (Groot et al., 1994, Sci. Hort., 59: 157-162). Additionally, reduced fertility is observed in the mutant, which also results in reduced productivity and that is the reason why the Is mutant does not reach any significance for the production oriented culture. An additional phenotypic change of the ls mutant is related to the formation of abscission zones in the flower and stems of the fruit. Whereas wild-type plants have a region of 5-10 layers of small cells, at the distant ends of which the non-pollinated flower or ripe fruit is separated from the plant (Robert et al., 1984, Planta, 160 : 159-163), this abscission zone is not formed in the Is mutant and during the harvest, the fruit separates from the plant without residues of sepals and fruit stalk. The observed phenotypic changes correlate with disorders in the equilibrium of the particular hormones in the plant at a physiological level. Compared to the wild type, the lower concentrations of cytokinin were measured at the tips of the suckers of the ls mutants (Maldiney et al., 1986, Physiol. Plant, 68: 426-430; Sossountzov et al., 1988, Plant , 175: 291-304), whereas the amounts of the compounds ß-indolyl acetic acid (IAA) as well as the guiberélico acids P1593 / 99MX and abscisic are markedly increased (Tucker, 1976, New Phytol., 77: 561-568). Attempts to remedy the deficiencies of the ls mutant by introducing an isopentenyl transferase gene from Agrobacterium um tumefaci ens resulted in an increase in endogenous cytokinin concentrations, but not a normalization of lateral shoot development (Groot et al. ., 1995, Plant Growth Regulation, 16, 27-36). Due to the great interest of the growers in individual stem tomato varieties, efforts have already been made to obtain the mutant ls usable for commercial cultivation. Since the DNA sequence of the gene (gene Ls) responsible for the formation of side shoots and / or formation of petals and / or formation of abscission zones has not been known, it has been tried repeatedly by genetic methods to separate the desired effects in the formation of lateral sprouts of unwanted effects on fertility and production. However, none of these efforts has been successful so far. For the isolation of genes that are only characterized by a mutant phenotype and their position in the genetic map, strategies of insertional mutagenesis and positional cloning have been used preferentially during the previous years. The insertional mutagenesis uses mutant alleles formed P1593 / 99MX by the insertion of a known sequence for the isolation of genes that are thus marked at a molecular level. In plants, the T-DNA of Agrobacterium um tumefaci ens (Koncz et al., 1992, Plant Mol. Biol., 30: 963-976) as well as the transposable elements (Gierl and Saedler, 1992, Plant Mol. Biol. , 19; 39-49) were used for insertional mutagenesis (Jones et al., 1994, Science 266: 789-793). Since these transposable elements Ac and Ds of maize are preferentially transferred to coupled positions on the same chromosome (Knapp et al., 1994, Mol. Gen. Genet., 243: 666-673) a transposon mutagenesis is particularly promising when it is A starting line is available in which the transposable element is present in close coupling with the gene of interest. Since this tomato line is not available; a transposon mutagenesis for the isolation of the Is gene is not very promising. The strategy for positional cloning was developed for the analysis of the molecular principles of hereditary diseases in mammals and inter alia was used for the isolation of human genes for Duchenne muscular dystrophy (Koening et al., 1987, Cell, 50: 509-517) Cystic Fibrosis disease (Roramens et al., 1989, Science, 245: 1059-1065) and Huntington's disease (Huntington's Disease Research Group, P1593 / 99MX 1993, Cell 72: 971-983). Figure 1 schematically illustrates the course of a positional cloning. For this strategy, the integration of the classical genetic locus into a map of molecular markers is of fundamental importance. The use of restriction fragment length polymorphisms (RFLP) as genetic markers (Botstein et al., 1980, Am. J. Hum. Genet., 32: 314-331) allows the identification of closely coupled DNA fragments of the environment of the gene to be isolated. These fragments subsequently serve as hybridization probes in Southern analysis by means of pulsed-field gel electrophoresis (Chu et al., 1986, Science, 234, 1582-1585) of separated DNA of high molecular weight, to transform the relative genetic distance in an absolute value for the physical distance that has to be joined by the so-called "displacement on the chromosome". Starting with flanking markers as starting points, the environment of the desired gene is isolated in the form of overlapping DNA fragments. Depending on the distance of the flanking markers in the genetic map the DNA fragments are YAC or cosmid clones (Burke et al., 1987, Science, 236: 806-812) RFLP maps with high density of markers have been developed by Nam et al. , 1989, Plant Cell, 1, 699-705 and Tanksley et al. , 1992, Genetics, 132: 1141-1160. Grill and Somerville, P1593 / 99 X 1991, Mol. Gen. Genet., 226: 484-490, and Martin et al. , 1992, Mol. Gen. Genet, 233: 25-32, describe the preparation of YAC libraries. In the classic genetic map of tomato, the locus Ls correlates on the long arm of chromosome 7 (Taylor and Rossall, 1982, Planta, 154: 1-5). Schumacher et al. , 1995, Mol. Gen. Genet, 246: 761-766, describe an integration of the Ls locus in the RFLP map, where the Ls locus was mapped within a range of 0.8 cM near the far end of chromosome 7. Additionally, Schumacher et al. describe that the Ls locus is linked by the RFLP, CD61 and CD65 markers. Physical mapping by pulse-field gel electrophoresis showed that CD61 and CD65 are not more than 375 kb apart from each other. With respect to the agricultural crop, the formation of side shoots is not desired in many economic plants due to several reasons: 1. The young side shoots are organs of "accumulation" (organs of consumption), and in this way, they reduce the production of the crop. main shoot. 2. Highly branched shoot systems often represent a harshly surmountable obstacle to mechanical treatment (eg machine-made coke). For these reasons, there have been attempts P159./99MX to cultivate in a conventional manner varieties without side shoots. This has been successful in individual economic plants (for example, sunflower). However, in many other economic, dicotyledonous plants (eg, tomato, cucumber, apple tree, pear), the individual stem would be desirable, but this has not been done so far in efficient crop varieties. Also in economical, monocotyledonous plants, such as, for example, corn and sugarcane, the formation of side shoots is advantageous and highly desirable for commercial use. At present, the individual stem, for example of the tomato, is achieved in common greenhouse cultivation in Central and Northern Europe by manually removing the lateral shoots. The removal of side sprouts can not be done with machines since this is associated with a huge cost. Additionally, at the injured site, the plants are very susceptible to pathogen infections, such as, for example, pathogenic bacteria, viruses and fungi. In this way, the removal of lateral suckers contributes to the spread of diseases in the greenhouse. However, in many ornamental plants, additional formation of lateral shoots is desired, and thus, an improved flower formation. The improved formation of side shoots is also highly beneficial in many economical plants, such as P1593 / 99MX for example, potato, coffee or tea plant. In this way, there is a need for economical, efficient, profitable plants and ornamental plants, in which the formation of lateral sprouts is increased or suppressed. The inhibition of the formation of abscission zones is of interest in several plants. In this way, the premature abscission of fruits in citrus plants results in production losses that could be prevented if these abscission zones were not formed. Similar results can be found in other fruit species, such as cherry, peach or black currant. Additionally, an inhibition of abscission zone formation is advantageous, for example, in tomato. If the abscission zones are not formed, the fruit is separated from the plant during the harvest without residues of sepals and fruit stalk. This characteristic is desired when tomatoes are cooked with machines and subsequently processed into products, such as, for example, tomato puree, since the sepals and the stems of the fruit deteriorate the quality of the tomato products. In ornamental plants, an increased formation of the abscission zones may be useful, since the flowers would fall on their own after fading and would not be necessary to manually remove them, as for example with many plants of P1593 / 99MX balcony and garden. If this does not happen, the formation of new flowers is suppressed.

BRIEF DESCRIPTION OF THE INVENTION The isolation and cloning of the Ls gene will offer the possibility of changing the gene in a sought way, and in this way, it will suppress or increase the formation of lateral shoots in transgenic plants. Additionally, the formation of abscission zones and / or petals can be suppressed or increased by changing the activity of the Ls gene in a sought-after manner. Accordingly, the fundamental object of the present invention is to isolate the Ls gene or a DNA fragment containing this gene, determine its sequence and provide a method for the preparation of transgenic plants in which the activity of the Ls gene of a sought way to suppress or increase the formation of side shoots and / or the formation of abscission zones and / or petals. The object of the present invention is solved by providing the nucleotide sequences according to SEQ ID NO: 1, 9 or 13, and the nucleotide sequences that hybridize to the nucleotide sequence according to SEQ ID NO: 1, 9 or 13, wherein the nucleotide sequences according to SEQ ID NO: 1, 9 or 13, and the nucleotide sequences that hybridize to the sequence of I >; I; QMY nucleotides according to SEQ ID NO: 1, 9 or 13 encode polypeptides that are responsible for the control of side shoot formation and / or the formation of petals and / or the formation of abscission zones. According to the present invention, the term "hybridization" is directed to conventional hybridization conditions, preferably "hybridization" is directed to these hybridization conditions in which the TM value is in the range of TM 45 ° C to TM 68 ° C. The term "hybridization" is directed in a particularly preferred manner to severe hybridization conditions. The invention further relates to polypeptide and amino acid sequences encoded by these nucleotide sequences. A further object of the invention is solved by a method for preparing plants having controlled formation of side shoots and / or formation of petals and / or formation of abscission zones, wherein the expressible DNA sequence, or fragment or derivative of the same, responsible for the control of the formation of side shoots and / or formation of petals and / or formation of abscission zones is integrated in a stable manner in the genome of the plant cells or tissues and the resulting plant cells or tissues are regenerated to form plants. In the present invention, a P1593 / 99MX method in which the integrated DNA suppresses the formation of side shoots and / or formation of petals and / or formation of abscission zones. Particularly preferred is a method in which the integrated DNA is expressed in an antisense orientation with respect to the complementary endogenous sequence which controls the formation of side shoots and / or formation of petals and / or formation of abscission zones. Also particularly preferred is a method in which the integrated DNA is expressed in a homosense orientation with respect to the endogenous, complementary sequence that controls the formation of side shoots and / or formation of petals and / or formation of abscission zones. Furthermore, a method in which the formation of side shoots and / or formation of petals and / or formation of abscission zones by a ribozyme comprising the DNA sequences or fragments or derivatives thereof according to the invention is particularly preferred. the present invention. Particularly preferred is also a method in which the DNA sequences or fragments or derivatives thereof according to the invention are used to disconnect ("knock down") the endogenous gene in plants by means of homologous recombination. In the present invention, a method is further preferred wherein the DNA integrated into the plant genome improves the formation of the P1593 / 99MX lateral shoots and / or formation of petals and / or formation of abscission zones. Particularly preferred is a method in which the DNA according to the invention is expressed in a homosense orientation with respect to the endogenous sequence responsible for the formation of side shoots and / or formation of petals and / or formation of abscission zones. Particularly preferred is the method according to the invention for the preparation of transgenic plants of tomato, rapeseed, potato or antirrhinum. Particularly preferred is also a method according to the present invention for the preparation of transgenic plants, wherein the DNA integrated into the plant genome comprises the sequence according to SEQ ID NO: 1, 9 or 13 or fragment or derivative of the same, or which is complementary to the sequence or fragment or derivative thereof, or which hybridizes with the sequence according to SEQ ID NO: 1, 9, or 13 or fragment or derivative thereof and which codes for a polypeptide having the biological activity of side shoot formation and / or formation of petals and / or formation of abscission zones. The invention further relates to transformed plant cells or tissue, wherein an expressible DNA sequence or fragment or derivative thereof responsible for controlling the formation of P1S93 / 99MX lateral shoots and / or formation of petals and / or formation of abscission zones integrates in a stable manner in the genome of the cell or plant tissue. Additionally, the invention relates to plants as well as to plant seed varieties obtainable according to the method of the present invention. The invention is further illustrated by the present figures, wherein: Figure 1 shows schematically the course of a positional cloning. Figure 2 illustrates in (a) a portion of the RFLP map published by Tanksley et al. , 1992, Genetics, 132: 1141-1160. In (b) the Ls region according to Schumacher et al. , 1995, Mol. Gen. Genet., 246: 761-766 is integrated into this map. Figure 3 shows the mapping of the cDNA and the cosmid clones of the Ls region. The cosmid clones A, B, C, D, E, F, G, and L as well as the clone CD61-5 of YAC are symbolized by bars. The positions of clO clO, c21 and 25 clones and cDNA are illustrated by open rectangles. Dashed lines represent recombination sites on plants 23, 24, 865 and 945 of F2. Figure 4 shows the autoradiography of a Southern blot analysis for the detection of genes related to Ls in different plant species. The genomic DNA of tomato P1593 / 99MX (Lycopersi with escul entum), potato. { Solanum tuberosum) and antirrhinum (An tirrhinum majus) was treated with restriction enzyme EcoRI and hybridized with the cDNA clone ET. Figure 5 shows the nucleotide sequence and the amino acid sequence derived therefrom (one letter code) of the wild type gene of tomato Ls (Lycopersi with escul entum). Figure 6 shows the nucleotide sequence and the amino acid sequence derived therefrom (one letter code) of the homologous gene Ls from the potato (Solanum tuberosum). Figure 7 shows the nucleotide sequence and the amino acid sequence derived therefrom (one letter code) of the 684 bp DNA fragment of the homologous gene of Ls from Arabidopsis thaliana. Figure 8 shows an alignment of the amino acid sequences of the Ls polypeptide from Arabidopsis thaliana (LsAt), Lycopersi with escul entum (LsLe) and Solanum tuberosum (LsSt). The one letter code was used for the amino acids. The identical amino acids are shaded in black, similar amino acids are shaded in gray. The hyphen (-) represents information of the missing sequence, a dot (.) Represents an additional amino acid in a polypeptide. An asterisk (*) represents a terminator codon at the level of nucleic acid.

P1593 / 99MX DETAILED DESCRIPTION PE THE INVENTION The method for cloning DNA fragments that are several hundred kilobases in length as chromosomes of artificial yeast (yeast artificial chromosome: YAC) in Saccharomyces cerevisiae (Burke et al., 1987, Science , 236: 806-812) allows the transformation of the physical map into a number of overlapping YAC clones encompassing the gel to be isolated. From a tomato YAC library (Martin et al., 1992, Mol. Gen. Genet., 233: 25-32) clones containing the CD61 marker of RFLP were isolated. By mapping the YAC terminal fragments with respect to the RFLP markers flanking the Ls gene as well as the recombination cleavage sites and the Ls gene itself, the position of the isolated DNA fragments in the Ls region was determined. In this way, YAC CD61-5 clone was found to hybridize with both CD61 and CD65, and therefore, contains the entire genomic region including the Ls gene. Figure 3 schematically illustrates the position of the marker and the YAC clone. For identification of the coding regions located within the YAC clone, this clone was used as a radiolabeled probe to probe a cDNA library (Simón, 1990, doctoral thesis, University of Cologne, Cologne, Germany). The cDNA library used is made from the RNA of both P1593 / 99MX the tips of the suckers floral as vegetative and in this way represents genes expressed from the tissues in which the phenotype of the Ls mutation manifests itself. A characterization of the cDNA clones by cross-hybridization revealed that the purified clones represented a total of 29 different transcripts. The subsequent fine mapping of the cDNA clones relative to the breakpoints of recombination in the CD61-CD65 range revealed that only clone y25 of cDNA co-segregated with the Ls gene and is a possible candidate for this gene. After the establishment of a contig (group of overlapping clones) of the cosmid which also contains cosmid clones as probes to isolate the additional cDNA clones from the CD61-CD65 range, that in the detection with the clone CD61-CD5 of YAC as a probe were not detectable due to the high complexity of the probe. In these experiments, three additional cDNA clones (clO, c21 and ET) were isolated which were also co-segregated with the Ls gene and were other possible candidates for the Ls gene. In this way, we identified a total of four cDNA clones from the Ls region, which were candidates for the Ls gene. In Figure 3, these clones are represented by open rectangles. In order to clone the Ls gene together with the promoter sequences necessary for expression regulation, clone y25 of cDNA was used as a P1S93 / 99 starting point for the isolation of the shorter genomic DNA fragments from the Ls region. For this purpose, a library of genomic cosmids was established from the tomato in vector pCLD04541 (Bent et al., 1994, Science, 265: 1856-1860). This vector contains the T-DNA border sequences necessary for the transformation of the plant, and in this way, allows an introduction of isolated DNA fragments into plant cells without additional cloning steps. From this library, a number of superimposed clones of cosmids were isolated in several typical cloning steps. The mapping of these cosmid clones relative to the recombination cleavage sites in the tested range showed that the isolated genomic DNA fragments encompassed a genomic region of approximately 60 kb. The position of the cosmid clones is illustrated schematically in Figure 3. To investigate the question whether a gene from the region of genomic DNA isolated as a contig of the cosmid is able to compensate for the biological function for the formation of lateral suckers, petals and abscission zones, which is absent in the mutant Is (complementation experiment), mutant Is was transformed with cosmids A, B, C, D, E, F, G and L clones. In all the transgenes elaborated by the introduction of cosmids A, B, C, D, E and F, no alteration of the phenotype could be observed. By contrast.

P1593 / 99MX in eight independent transgenic plants containing either the cosmid G or L, a partial or complete recovery of the wild type phenotype could be observed. The results of the complementation experiments are illustrated in Table I.

Table I: Complement experiments of the mutant Is via the transformation of cosmids These transgenic plants form lateral shoots during the vegetative development and also again petals and zones of abscission in the floral development. A Southern blot analysis of the transgenic plants containing the cosmid G or the cosmid L revealed that in plants that do not show complementation the T-DNA was only P1593 / 99MX transferred incompletely. In this way, it has been shown that the introduced DNA fragments are able to complement the genetic information for the formation of lateral shoots, petals and zones of abscission, which is absent from the mutant. By using the complementation experiments with sub-fragments of the cosmid G, the region of DNA in which the Ls gene is localized can be determined in more detail. While after the transformation with DNA fragments containing the previously identified c21 gene, the complementation of the ls phenotype could not be observed, the wild type phenotype could be recovered in eight independent transgenic plants by introducing a 6 kb fragment that It has the ET gene. A DNA sequence analysis revealed that the ET gene of the ls1 mutant harbors a 1550 bp deletion that removes the first 185 amino acids of the protein and 865 bp of the sequence that is located upstream. A second independent mutant ls2 allele contains a 3 bp insertion and several dot mutations in a short portion of DNA, one of which results in a protein termination after 24 amino acids. The complementation experiments and the isolation and correlation of the cDNAs as well as the sequence analyzes of the wild type ET gene and of two independent ls alleles revealed that the ET cDNA clone represents the sequence of Pi? 93 / 99M -? - complete coding of the ls gene mRNA. To address the question of whether similar or homologous genes are present in other plant species as well, the cDNA clone ET was used as the hybridization probe in the Southern experiments under reduced severity. The term "plant" as used herein, comprises ornamental and economic, monocotyledonous and dicotyledonous plants. The term "reduced severity", as used herein, refers to typical hybridization conditions with the modification that the hybridization temperature was between 50 ° C and 55 ° C. In the potato (Solanum tuberosum) and the antirrhinum (An tirrhinum majus) several DNA fragments could be detected. From the antirrhinum, several genomic clones were isolated by hybridization at 55 ° C. A DNA sequence analysis revealed that the isolated antirhino clone has significant sequence homologies to the Ls gene. In this manner, genes homologous to the tomato Ls gene can be isolated according to conventional methods by using the cDNA clone ET as a probe. Using gene-specific primers, the homologous gene of Ls was isolated from the genomic DNA of the potato (Solanum tuberosum) via PCR. The homologous gene Ls of the potato shows a sequence identity of approximately 98% to the Ls gene of the tomato at the DNA level as well as at the protein level. Starting P1593 / 99MX of the Arabidopsis genomic DNA (Arabidopsis thaliana) was isolated a 687 bp DNA fragment of the Ls homologous gene via PCR using degeneration primers. At the DNA level, the DNA fragment of Arabidopsis thaliana exhibits a sequence identity of approximately 63% to the Ls gene of the tomato. At the protein level they are identical for approximately 55% of the amino acids. The present invention is further directed to DNA sequences that are derived from a plant genome and encode a protein necessary to control the formation of side shoots and / or formation of petals and / or formation of abscission zones. In the introduction and expression in plant cells, the information contained in the nucleotide sequence results in the formation of a ribonucleic acid. By means of this ribonucleic acid, a protein activity can be introduced into the cells or an endogenous protein activity can be suppressed. Particularly preferred is a DNA sequence according to SEQ ID NO: 1 from Lycopersicon esculentum shown in Figure 5, a DNA sequence according to SEQ ID NO 9 from Solanum tuberosum shown in Figure 6 and a sequence of DNA according to SEQ ID NO: 13 from Arabidopsis thaliana shown in Figure 7. Further, the present invention is P1593 / 99 X refers to the use of DNA sequences or fragments or derivatives according to the present invention that are derived from the DNA sequences by insertion, deletion or substitution in the transformation of plant cells. The DNA sequences according to the present invention can be employed using different methods to suppress the formation of lateral shoots, and thus, of the ramifications of the shoot and / or petal system and / or abscission zones: 1. For suppressing the formation of side shoots and / or petals and / or abscission zones, the DNA sequence according to the present invention can be cloned and in an antisense orientation or a homosense orientation in conventional vectors (eg, plasmids), and in this way, combined with control elements for expression in plant cells, such as, for example, promoters and terminators. By using the prepared vectors, the plant cells can be transformed in order to prevent the synthesis of the endogenous protein. For this purpose short portions of the DNA sequence according to the invention can also be used, ie fragments or DNA sequences having sequence similarity from 50% to 100%, ie, derivatives. In this way, the homologous gene Ls derived from Arabidopsis can be used for example to suppress P1593 / 99MX the formation of lateral shoots, and in this way, ramifications of the system of shoots and / or petals and / or zones of abscission in related species of Brassi ca napus (rapeseed). The targeted suppression of a genetic activity in plant cells by the introduction of antisense or homosense constructs is a common method that has been used successfully in many cases (Gray et al., 1992, Plant, Mol. Biol., 19: 69-87). 2. Additionally, the formation of side shoots and / or petals and / or abscission zones can be initiated by expressing a ribozyme constructed for this purpose using the DNA sequence according to the present invention. The preparation and use of ribozymes is described in de Feyter et al. , 1996, Mol. Gen. Genet., 250: 329-338 for tobacco and tomato plants resistant to tobacco mosaic virus. 3. Additionally, the DNA sequence according to the present invention can be used to inactivate the endogenous gene. By using the DNA sequences of the present invention, the oligonucleotides can be synthesized to test the plants in the context of the mutagenesis experiments by means of the PCR technique for the presence of inserts (eg, transposable elements or the T- DNA of Agrobacterium turn faci ens) in the Ls gene. In general, genetic activity will be blocked by these insertions (Koes et al., 1995, Proc. Nati. Acad.

Pl? Qi / QQMY Sci. USA, 92: 8149-8153). 4. The DNA sequence according to the invention can also be used to disconnect ("knock down") the endogenous Ls gene by means of homologous recombination. This method was used successfully in mice and is also described for use in plants by Miao and Lam, 1995, Plant. J., 7, 359-365. In contrast to tomato and other economic plants, in ornamental plants (for example, geraniums, fuchsias and chrysanthemums) phenotypes that exhibit a dense growth due to strong development of lateral shoots are often preferred. In order to generate these forms of growth at the present time, the plants are decapitated, which promotes the beginning of the lateral axes, or they are treated with particular chemical products. However, this practice is also associated with considerable costs. In these cases, the preparation of transgenic plants having bushy growth forms according to the present invention represents a more profitable alternative. In ornamental plants, an improved formation of abscission zones can be used in such a way that after the fading the flowers detach by themselves and should not be removed manually as with many balcony and garden plants. If this does not happen, it is frequently deleted P1? Q? / qqMY the formation of new flowers. For the preparation of transgenic plants, with a strong formation of side shoots and / or strong formation of abscission zones, the DNA sequence or fragment or derivative thereof, according to the invention, which is derived from the sequence by insertion, deletion or substitution, it is introduced into plasmids in a homosense orientation and combined with the control elements for expression in plant cells. Using these plasmids, the plant cells can be transformed in such a way that a messenger, translatable ribonucleic acid (mRNA) is expressed that allows the synthesis of a protein that stimulates the formation and development of side shoots and / or petals and / or zones. of abscission. The DNA sequence or fragments or derivatives thereof, according to the present invention, which are derived from this sequence by insertion, deletion or substitution can be used to isolate homologous or similar DNA sequences from the tomato genome or other plants, these DNA sequences also influence the formation of side shoots and / or petals and / or abscission zones. For this purpose, the DNA sequence or fragments, for example oligonucleotides, or derivatives, according to the present invention, can be used as probe molecules for probing P1593 / 99MX cDNA libraries or genomic DNA libraries of the plants to be probed according to conventional methods. Alternatively, degenerate or non-degenerate oligonucleotides (primers) can be derived from the sequence according to the present invention, which can be used to probe these cDNA libraries or genomic DNA libraries, on a PCR basis. Similar to the DNA sequences according to the present invention, the isolated, related DNA sequences can be used for the inhibition or stimulation of side shoot formation and / or formation of petals and / or formation of abscission zones in plants. For the expression of the DNA sequences according to the present invention, in the homosense or antisense orientation in plant cells, on the one hand, the transcription promoters are necessary, and on the other hand, the transcription terminators are necessary. Several promoters and terminators have been described in the literature (eg Koster-Tpfer et al., 1989, Mol Gen. Genet., 219: 390-6, Rocha-Sosa et al., 1989, EMBO J., 8:23 -29). The transcriptional initiation and termination regions can be derived either from the host plant or from a heterologous organism. The DNA sequences of the initiation and transcription and termination regions of pmQI / QQMV transcript can be prepared synthetically or obtained naturally or can contain a mixture of the synthetic and natural components of DNA. Methods for genetic modification have been described for dicotyledonous and monocotyledonous plants (Gasser and Fraley, 1989, Science 244: 1293-1299, Potrykus, 1991, Ann.Rev. Plant.Mol. Biol. Plant Physiol., 42: 205- 226). In addition to transformation through Agrobacterium um tumefaci ens (Hoekema, 1983, Nature, 303: 179-180, Filatti et al., 1987, Biotech, 5; 726-730), DNA can be introduced by protoplast transformation, microinjection , electroporation or ballistic methods in plant cells. For the selection of transformed plant cells, the DNA to be introduced is coupled with a selection marker that imparts antibiotic resistance (eg, kanamycin, hygromycin, bleomycin) to the cells. From the transformed plant cells, the whole plants can then be regenerated in a typical selection medium. The regeneration of plant cells is described, for example in EP-B-0 242 236, which is incorporated herein by reference. The plants obtained in this way are tested for the presence and integrity of the introduced DNA by means of conventional molecular biological methods. Once the introduced DNA is integrated into the genome, it is generally P1593 / 99MX stable and transmitted to descendants. By using conventional methods, seed varieties can be obtained from the resulting plants. The following examples are proposed to illustrate the present invention and should not be considered as limiting. If not otherwise mentioned, normal, biological, molecular procedures were used, as described by Sambrook et al. , 1989, Molecular Cloning: A Laboratory Manual, 2nd Edition, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York. Southern hybridizations were carried out in 6 x SSPE (0.9 M NaCl, 50 mM NaH2P04 x H20, 5 mM EDTA, 0.1% BSA, 0.1% Ficoll, 0.1% PVP, 0.5% SDS, 100 μg / ml DNA of calf thymus) with a Hybond N + membrane (Amersham). Hybridizations were made in plate in 6 x SSPE (1.08 M NaCl, 60 mM NaH2P04 x H20, 6 mM EDTA, 0.1% BSA, 0.1% Ficoll, 0.1% PVP, SDS at 0. 1%, 200 μg / ml of horse thymus DNA) with a Hybond N + membrane (Amersha).

EXAMPLE 1 Isolation of YAC clones from the tomato Ls region From a tomato YAC library (Martin et al., 1992, Mol, Gen, Genet., 233: 25-32) clones containing the CD61 marker were isolated (Schumacher et al., 1995, Mol. Gen. Genet., 246; 761- P1593 / 99MX 766). For this, DNA mixtures that were derived from a micro-titer plate with 96 YAC clones were first tested using the conventional PCR method. Thus, out of the 144 of these DNA mixtures, it could be identified that nine provide a PCR product with the CD61-F and CD61-R primers (Schumacher et al., 1995, Mol. Gen. Genet., 246: 761-766). The isolation of the individual clones was carried out by means of colony hybridization or PCR, where the DNA of the clones of a row or column of a micro-titer plate was used as a mixture. In this way, from the 96 clones of a plate, individual clones were identified using 20 PCR reactions. In total, five YAC clones were identified, the insert size of which was determined and is 280-320 kb by pulsed field gel electrophoresis (Chu et al., 1986, Science, 234: 1582-1585). It was shown in PCR and in Southern experiments that YAC CD61-5, in addition to CD61, also carries the second flanking marker CD65 in this manner encompasses the Ls locus.

EXAMPLE 2 Isolation of cDNA clones from the tomato Ls region For the preparation of a hybridization probe DNA, the clone CD61-5 YAC was isolated after separation by means of gel electrophoresis Pl RQI / QQ V pulse field. Nevertheless, pulse field gel separation allowed only a relatively rough preparation, so that the probe used, in addition to clone YAC CD61-5 also contained portions of the DNA from chromosome III (360 kb) and VI (280 kb ) of yeast. Following the radiolabel, this DNA was used as a probe to probe 5 x 10 5 pfu (plaque forming units) in a conventional plate hybridization. Hybridization with the YAC probe provided a plurality of signals of different intensity. To re-probe 50 plates of different signal intensities, 44 clones were selected and purified and then grouped by cross-hybridization. 23 of the 44 clones that resulted from the re-detection were present only once. In total, 29 different transcripts were identified in this survey. After establishment of a cosmid contig, the cDNA library was again probed with the cosmid clones to isolate additional cDNA clones that were not amenable to detection with YAC61-5 as a probe due to the high complexity of the probe. In these experiments, three additional cDNA clones were found. A total of 32 different transcripts were detected. pi? q ^ í QQMV EXAMPLE 3 Mapping of RFLP of cDNA clones isolated from tomato From 30 identified transcripts, 22 showed typical hybridization patterns for single or low copy sequences that allowed the correlation of RFLP. In a first analysis of RFLP, the isolated cDNA clones were hydrized against filters carrying the L DNA. escul en tum, L. pennellii, as well as from the IL83 backcross line digested with the EcoRI, EcoRV and Xbal endonuclease restriction enzymes (Eshed et al., 1992, Theor.Appl. Genet, 83: 1027-1034). This line, in which the distant term of chromosome 7 is derived from L. pennellii while the rest of the genome is composed of the chromosomes of L. esculentum, allows a first approximate correlation in the presence of a polymorphism between L. escul en tum y L. pennellii. If a fragment of polymorphic DNA was derived from the Ls region, the IL83 line exhibited the allele of L. pennellii, while the allele of L. esculentum was present for fragments of the remaining genome. In this way, four cDNA clones were identified that were not derived from chromosome 7. The fine mapping of the remaining 18 cDNA clones derived from chromosome 7 was carried out via the RFLP analysis of plants W23 and W24 that contained cases of recombination in the range CD61-I, s and I, s-CD65, respectively.

P1593 / 99MX Since in this analysis, candidates for the Ls gene in the W23 plant exhibited the L-specific fragment. escul in tum as well as specific to L. pennellii, whereas in the W24 plant only the L-specific fragment was present. esculentum, the cDNA clones were hybridized against filters having the DNA digested with EcoRI, EcoRV or Xbal of both genetically related species, as well as both recombinants W23 and W24. In this way, we identified a total of four cDNA clones that were co-segregated with the Ls gene, and in this way, candidates for the Ls gene were possible.

EXAMPLE 4 Preparation and probing of a tomato genomic cosmid library The DNA of the T-DNA / cosmid vector pCLD04541 (Bent et al., 1994, Science, 265: 1856-1860) was isolated according to the protocol of Sambrook et al. al , 1989, Molecular Cloning: A Laboratory Manual 2d Edition, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York, was purified via two gradients of CsCl and dialyzed against TE for 3 days. The DNA was completely digested with BamHI and subsequently dephosphorylated with alkaline phosphatase to prevent self-ligation of the vector. 200 ng of the genomic tomato DNA partially digested with Mbol and 2 mg of the vector DNA were ligated with T4 DNA ligase in 10 ml a P1593 / 99MX 16 ° C overnight. 3 ml of the ligation assay was used for packaging and transfected into SUR.E E. coli (Stratagene). This assay resulted in 6 x 106 independent recombinant bacteria. Each of the 100 plates was plated with 2500 ufe (colony forming units) and rinsed thoroughly with 10 ml each of the LB medium. In each case, a glycerol culture was made from this material and a DNA preparation was carried out. These 100 DNA mixtures were probed by PCR analysis. The positive mixtures were then subjected to a colony filter hybridization to identify the individual, positive clones.

EXAMPLE 5 Cloning and sequencing of the tomato Ls gene The cDNA ET clone insert that was isolated as a probe in the probe of the cDNA library with the cosmid G was cut with EcoRI and cloned into the vector pGEM-llZf (+ ). The missing 5 'of the gene was isolated by means of the RACE technique (Frohman et al., 1988, Proc. Nati, Acad. Sci. USA 85: 8998-9002). Here, starting from an oligonucleotide that specifically binds to known regions of the gene, a DNA complementary to the RNA (cDNA) was prepared. The deoxycytosine nucleotides were subsequently linked to the cDNA using terminal transferase.

P1"i93 / P9MY With a second primer specific for the gene and a primer that binds to the tail of the polydeoxycytosine, the 5 'end of the cDNA was amplified via PCR and cloned into the plasmid vector pGEM-T. The longest of the RACE clones were sequenced Simultaneously with the cDNA ET clone analysis, sub-fragments of the respective genomic region of cosmid G were isolated and recloned into the plasmid vectors pGEM-4Z and pSPORTI. the overlapping or overlapping sub-fragments The genomic sequence showed no difference in the sequence of the cDNA clone, which means that the Ls gene does not contain any intron.In addition, the respective genome regions of both ls1 and ls2 mutants were amplified of the genomic DNAs via PCR using suitable primers and cloned into the pGEM-T vector The sequence analysis of these products received a 1.5 kb deletion in the Is1 allele in comparison to the wild type sequence. In addition to the loss of nucleotides 1-685 from the open reading frame, the ls1 mutant also lacks 865 base pairs from the region located 5 'of the open reading frame, which is thought to have the regulatory (promoter) function for The expression. Therefore, it can be assumed that the ls1 mutant is not able to form a functional protein of the Ls gene any longer. In the ls2 allele, an insertion of 3 Pairs P1593 / 99MX of bases as well as exchanges of 3 bases were found in the 5 'region of the open reading frame. One of these base changes led to a terminator codon resulting in a termination of the amino acid chain after 24 amino acids. Again, a protein without any function will be assumed. Vectors pGEM-llzf (+), pGEM-4z, pGEM-T were purchased from Promega Corp., Madison, U.S. A., the pSPORTI vector was purchased from Life Technologies, Eggenstein, and used according to the manufacturer's instructions.

EXAMPLE 6 Transformation of Plants with Mapping of Ls cDNA Constructs from Tomato The Ls cDNA was isolated with the gene-specific primers, CD61-13 (5'-TTAGGGTTTTCACTCCACGC-3 '; SEQ ID NO: 3) and CD61-28 ( 5'-TCCCCTTTTTTTCCTTTCTCTC-3 '; SEQ ID NO: 4) by means of the conventional PCR method were cloned into the plasmid vector pGEM-4z (GSET8). For the preparation of the transformation constructs, the Ls cDNA was cut from the plasmid GSET8 with Sall / SstI (for homosentide construction) and with Xbal / Sstl (for antisense construct) it was ligated into the pBIR vector of transformed plant digested with SalI. / SstI (homosentido construction) and with Xbal / Sstl (antisense construction), respectively P1593 / 99MX (Meissner, 1990, doctoral thesis, University of Cologne, Cologne). In the resulting clones, the cDNA is present in either the homosense orientation or the antisense orientation between the promoter and the polyadenylation site of the 35S gene of the cauliflower mosaic virus. The resultant homosentide and antisense plasmids were introduced into the strain GV3101 of Agrobacterium tumefaciens (Koncz and Shell et al., 1986, Mol.Gen.Genet., 204: 383-396) by direct transformation. Subsequently, the T-DNAs of the two different constructions were transformed into pieces of tomato leaf and tobacco according to Fillatti et al. , 1987, Biotech, 5: 726-730. Different transgenic plants containing the antisense construct of Ls showed a reduction in the formation of lateral shoots.

EXAMPLE 7 Isolation of a Ls-related gene from the antirrhinum (.Antirrhinum majus) With the ET cDNA clone as a probe, a genomic phage library of Antirrhinum majus was detected. Hybridization was carried out at 55 ° C, that is, under reduced severity. In this experiment, 14 clones were isolated, the clone HH13 of which showing the strongest hybridization signals was further characterized. The analysis of the sequence carried out after the P1593 / 99MY recloning the phage insert in the plasmid vector pGEM-llzf (+) showed that the gene isolated from An tirrhinum majus has high homology in sequence to the Ls gene of the tomato. Within both regions, regions can be identified, in which the sequence derived from amino acids is fully conserved.

EXAMPLE 8 Isolation of a Ls-related gene from potato (Solanum tuberosum) In a Southern blot experiment under reduced severity at 55 ° C using Ls gene cDNA as a hybridization probe, a DNA fragment could be detected in the Genomic DNA in Solanum tuberosum (Figure 4). Using the gene-specific primers CD61-24 (5 '-TTTCCCACTCAAGCCAACTC-3'; SEQ ID NO: 5), CD61-6 (5'-GGTGGCAATGTAGCTTCCAG-3 '; SEQ ID NO: 6), P01 (5' -TCGAGGCGTTGGATTATTATAC -3 '; SEQ ID NO: 7) and P05 (5' -GGCCCCCATATCTTTTTCC-3 '; SEQ ID NO: 8) were isolated genomic DNA fragments, which overlap the Ls gene, from the DNA conventionally isolated from Solanum tuberosum when using the PCR method. The PCR reactions were carried out as follows: denaturation at 95 ° C for 30 seconds, fixed at 60 ° C for 1 minute, elongation at 72 ° C for 2 minutes. This cycle was repeated 30 times. The resulting PCR products are P1593 / 99MX cloned into the plasmid vector pGEM-T. A sequence analysis revealed that DNA fragments isolated from Solanum tuberosum have the sequence information for an open reading frame that has a coding capacity of 431 amino acids (Figure 6). The DNA sequence is shown in SEQ ID NO: 9 and the amino acid sequence encoded with the DNA sequence is illustrated in SEQ ID NO: 10. At the level of the DNA as well as at the level of the protein, the homologous gene of Ls of the potato exhibits a sequence identity of approximately 98% to the Ls gene of the tomato.

EXAMPLE 9 Isolation of a gene related to Ls from Arabidopsis thaliana For the isolation of the homologous gene of Ls from Arabidopsis thal iana, the degenerate primers CD61-38 (5 '-CARTGGCCNCCNYTNATGCA-3'; SEQ ID NO: 11) * and CD61-41 (5 '-TGRTTYTGCCANCCNARRAA-3'; SEQ ID NO: 12) * were made and used for PCR reactions with genomic DNA from Arabidopsis thaliana isolated in the usual manner. The PCR reactions were carried out as follows: denaturation at 95 ° C for 30 seconds, fixed at 50 ° C for 1 minute, elongation at 72 ° C for 1 minute. This cycle was repeated 35 times. In this way, it was possible to amplify a DNA fragment of approximately 700 bp that P1593 / 99MX was subsequently cloned into the vector pGEM-T. A sequence analysis showed that the DNA fragment isolated from Arabidopsis thaliana (SEQ ID NO: 13) was 687 bp in length and has high sequence similarity to the Lycopersi Ls gene with esculentum. At the DNA level, the DNA fragment of Arabidopsis thaliana showed a sequence identity of approximately 63% to the Ls gene of the tomato. At the level of the protein, approximately 55% of the amino acids are identical. The amino acid sequence encoded by the isolated DNA fragment (SEQ ID NO: 13) is illustrated in SEQ ID NO: 14. By using the isolated DNA fragment the homologous gene of Arabidopsis thaliana Ls can be isolated using normal molecular biological methods conventional * In the description of the degenerate primers, the WIPO St 23 standard were used: R = A + G N = A + G + C + T Y = C + T P1593 / 99MX LIST OF SEQUENCES (1) GENERAL INFORMATION: (i) APPLICANT: (A) NAME: Nikolaus (Klaus) Theres (B) STREET: Schiffgesweg 30 (C) CITY: Pulheim (D) STATE: NRW (E) COUNTRY: Germany (F) CODE POSTAL: 50259 (G) TELEPHONE: + 49 2234 89386 (ii) TITLE OF THE INVENTION: PLANTS WITH CONTROLLED TRAINING OF LATERAL CHALLENGES AND / OR CONTROLLED TRAINING OF ABSCISION ZONES (iii) NUMBER OF SEQUENCES: 14 (ív) READABLE FORM COMPUTER: (A) TYPE OF MEDIUM: Flexible disk (B) COMPUTER: IBM compatible PC (C) OPERATING SYSTEM: PC-DOS / MS-DOS (D) SOFTWARE: Patentln Relay # 1.0, Version # 1.30 (EPA) (2) INFORMATION FOR SEQ ID NO: 1: (i) CHARACTERISTICS OF THE SEQUENCE: (A) LENGTH: 1729 Pairs of bases (B) TYPE: Nucleotide (C) TYPE OF HEBRA: double (D) TOPOLOGY: linear (ii) ) TYPE OF MOLECULE: cDNA (iii) HYPOTHETIC: NO (vi) ORIGINAL SOURCE: (A) ORGANISM: Lycopersicon esculentum (xi) DESCRIPTION OF SEQUENCE: SEQ ID NO: 1: CTICTGTCCT TCCCCCCAGG TCCCCTT-TT TI CTTTCTC TCTCICCTTT ATTTCTCT-T 60 TCATAAGCAT ATTC1TTCTC TCTCTAGGGT TT-X? CTTTC ACCTGAAATA GTGT1GTTAA 120 ATTGAATG? T ATGTTAGGAT CCTTGGGTTC T_X_ TCATCT CAATCTCACC CTCATCATGA 180 TGAAGAATCT TCOX-ATCATC ATCAACAGCG TAGATTCACC GCTACTGCTA CAACTATCAC 240 P1593 / 99MX CACCACCACC ATCACGACCT C? CCAGC AT TCAAATCCGC C? GCTACTCA TGAGCTGTGC 300 GGAGTTGATT TCGCAGTCCG ATTTCTCGGC CXKGAA? AGA CTCCTGACTA TATTATCAAC 360 TAACTGATCT CCT I'IGG G ATICAACTGA AO_GTGAGTC CKSC? A? RTA CTCGCGCACT 420 TICCC? CGG CTCA? CCGCT ATATATCG C AACCACCAAT C? TTTCATGA (ZACCTGTTGA 480 AACAACTCCA ACTGATTCTT L ICT'ICGTC ATC? TTAGCT CTAATTCAAT CATCATATCT 540 ATCTCTAAAC CAAGTTACCC CTTDCATAAG GTITACTCAA TTAACCGCTA ATCAAGCGAT 600 TTTAGAAGCG ATTAACGGTA ATCATCAAGC AATCCACATC GTTCATTTCG ACATTAATCA 660 Oi rTC ?. TGGCC? CCGT TAATGCAAGC ACTAGCTG? T apACCCTG CTCCCACTCT 720 TCGAATCACC GGTACTGGAA ATGACCTTGA TACCCTTCGT AGAACAGGG ATCGTTTAGC 780 TA? ATTTGCT (? CTCATTAG GGTTGAGATT TO-ATTCCAT CCTCTTTATA TAGCCAATAA 840 TAACCACGAT CACX3ATGAAG ATCCTTCTAT TATITCCTCC ATTGTACTAC TCCCTGATGA 900 AACCCTAGCT ATCAACTGIG TITICTACCT OR? CCGCCTT TTAAAAGACC GCGAAAAGTT 960 AAGGA1TTTT TTGCATAGGG TTAAGTCAAT GAACCCTAAA ATTGTTACAA TCGCGGAGAA 1020 GGAAGCAAAT CATAACCATC CT i lTi ACAAAGATTC ATCGAGGCGT TGGATTATTA 1080 TACAGCTGTG TTIGATTCAC TGGAAGCTAC ATTGCCACCG GGTAGTCGAG AGAGGATGAC 1140 AGTIGAACAA GTGTCGTTTG GGAGAGAGAT TGTTGATATC GITGCGATGG AAGGAGATAA 1200 AAGGAAAGAA AGACATGAAA GGTTTAGATC ATGGGAAGTT ATGTIGAGGA GT.X_TGGA.TT 1260 TAGTAATGTT GCTTTAAGCC CT1 1GCATT ATC? CAAGCT AAGCTTCTTT TGAGACTTCA 1320 t _xxttct GAAGGCTATC AACTCGGAGT TTCGAGTAAT TCTTTCGICT TAGGTTGGCA 1380 AAATCAACCC CTTTTCTCCA TCTCX3TCTTG GCGTTCAGAA AAACTATCAA ATAGCCAACT 1440 TC? GAGGGTA ATTAAGACTA CTGATAGTTT AGGAGGGATC TGAAGAAAAC GCGTGGAGTG 1500 AAAACCCTAA ATAACCAGAT TT1CTAA.TG? AGTTGTAGTA GTAGAAATTT GCATC ^ GTGAA 1560 GAACAATATT GAAGAGGTAT TGAAATITCA TGTTTITTTT GTTTTACTTA TTGATATGAA 1620 TGTTTTAAAA TTT-TAACAT AGAGGACTAG GTTGATGATA TATAGTATTT AAGTTAACTA 1680 G-X-TTTGTAT AACGCAAGAT CITGATCAAC TTATTTTTAT TITTAATTA 1729 (2) INFORMATION FOR SEQ ID NO: 2: (i) CHARACTERISTICS OF THE SEQUENCE: (A) LENGTH: 428 amino acids P1593 / 99MX (B) TYPE: amino acid (C) TYPE OF HEBRA: individual (D) TOPOLOGY: linear (ii) TYPE OF MOLECULE: Protein vi) ORIGINAL SOURCE: (A) ORGANISM: Lycopersicon esculentum (xi) DESCRIPTION OF THE SEQUENCE: SEQ ID NO: 2 Met Leu Gly Being Phe Gly Being Being Ser Gln Ser His Pro His His 1 5 10 15 Asp Glu Glu Being Asp His His Gln Gln Arg Arg Phe Thr Wing Thr 20 25 30 Wing Thr Thr lie Thr Thr Thr Thr Thr Pro Pro Wing lie Gln 35 40 45 lie Arg Gln Leu Leu lie Ser Cys Wing Glu Leu lie Ser Gln Ser Asp 50 55 60 Phe Be Wing Wing Lys Arg Leu Leu Thr lie Leu Ser Thr Asn Ser Ser 65 70 75 80 Pro Phe Gly Asp Ser Thr Glu Arg Leu Val His Gln Phe Thr Arg Wing 85 90 95 Leu Ser Leu Arg Leu Asn Arg Tyr lie Ser Thr Thr Asn His Phe 100 105 110 Met Thr Pro Val Glu Thr Pro Thr Asp Ser Ser Ser Ser Ser 115 120 125 Leu Ala Leu Lie Gln Ser Ser Tyr Leu Ser Leu Asn Gln Val Tíhr Pro 130 135 140 Phe lie Arg Phe Thr Gln Leu Thr Wing Asn Gln Wing He Leu Glu Wing 145 150 155 160 lie Asn Gly Asn His Gln Wing He His He Val Asp Phe Asp He Asn 165 170 175 His Gly Val Gln Trp Pro Pro Leu Met Gln Ala Leu Wing Asp Arg Tyr 180 185 190 Pro Wing Pro Thr Leu Arg He Thr Gly Thr Gly Asn Asp Leu Asp Thr 195 200 205 Leu rg Arg Thr Gly Asp Arg Leu Wing Lys Phe Wing His Ser Leu Gly 210 215 220 Leu Arg Phe Gln Phe His Pro Leu Tyr He Wing Asn Asn Asn His Asp 225 230 235 240 / 9_9MX His Asp Glu Asp Pro Ser He He Ser Ser He Val Leu Leu Pro Asp 245 250 255 Glu Thr Leu Ala He Asn Cys Val Phe Tyr Leu His Arg Leu Leu Lys 260 265 270 Asp Arg Glu Lys Leu Arg He Phe Leu His Arg Val Lys Ser Met Asn 275 280 285 Pro Lys He Val Thr He Wing Glu Lys Glu Wing Asn His Asn His Pro 290 295 300 Leu Phe Leu Gln Arg Phe He Glu Wing Leu Asp Tyr Tyr Thr Wing Val 305 310 315 320 Phe Asp Ser Leu Glu Wing Thr Leu Pro Pro Gly Ser Arg Glu Arg Met 325 330 335 Thr Val Glu Gln Val Trp Phe Gly Arg Glu He Val Asp He Val Wing 340 345 350 Met Glu Gly Asp Lys Arg Lys Glu Arg His Glu Arg Phe Arg Ser Trp 355 360 365 Glu Val Met Leu Arg Ser Cys Gly Phe Ser Asn Val Ala Leu Ser Pro 370 375 380 Phe Ala Leu Ser Gln Ala Lys Leu Leu Arg Leu His Tyr Pro Ser 385 390 395 400 Glu Gly Tyr Gln Leu Gly Val Ser Ser Asn Ser Phe Phe Leu Gly Tcp 405 410 415 Gln Asn Gln Pro Leu Phe Ser lie Ser Trp Arg 420 425 (2) INFORMATION FOR SEQ ID NO: 3: (i) CHARACTERISTICS OF THE SEQUENCE: (A) LENGTH: 20 Pairs of bases (B) TYPE: Nucleotide (C) TYPE OF HEBRA: individual (D) TOPOLOGY: linear (ii) ) TYPE OF MOLECULE: DNA s intéti co (iii) HYPOTHETIC: NO (xi) DESCRIPTION OF SEQUENCE: SEQ ID NO: 3: OTAGGGTTTT CACTCCACGC 20 P1593 / 99MX (2) INFORMATION FOR SEQ ID NO: 4: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 22 Base pairs (B) TYPE: Nucleotide (C) TYPE OF HEBRA: individual (D) TOPOLOGY: linear (ii) TYPE OF MOLECULE: synthetic DNA (iii) HYPOTHETIC: NO (xi) DESCRIPTION OF SEQUENCE: SEQ ID NO: 4 TCCC IT G -TCCGTTCTC TC 22 (2) INFORMATION FOR SEQ ID NO: 5: (I) CHARACTERISTICS OF THE SEQUENCE: (A) LENGTH: 20 Pairs of bases (B) TYPE: Nucleotide (C) TYPE OF HEBRA: individual (D) TOPOLOGY: linear (ii) ) TYPE OF MOLECULE: synthetic DNA (iii) HYPOTHETIC: NO (xi) DESCRIPTION OF SEQUENCE: SEQ ID NO: 5: THCCCACTC AAGCCAACTC 20 (2) INFORMATION FOR SEQ ID NO: 6: (i) CHARACTERISTICS OF THE SEQUENCE: (A) LENGTH: 20 Pairs of bases (B) TYPE: Nucleotide (C) TYPE OF HEBRA: individual (D) TOPOLOGY: linear (ii) ) TYPE OF MOLECULE: synthetic DNA (iii) HYPOTHETIC: NO (xi) DESCRIPTION OF SEQUENCE: SEQ ID NO: 6: GGTGGCAATG TAGCTTCCAG 20 (2) INFORMATION FOR SEQ ID NO: 7: P1593 / 99MX (i) CHARACTERISTICS OF THE SEQUENCE: (A) LENGTH: 22 Pairs of bases (B) TYPE: Nucleotide (C) TYPE OF HEBRA: individual (D) TOPOLOGY: linear (ii) TYPE OF MOLECULE: DNA s intéti co (i ii) HYPOTHETIC: NO (xi) DESCRIPTION OF THE SEQUENCE: SEQ ID NO: 7: TCXIAGGCGTT GGATTATTAT AC 22 (2) INFORMATION FOR SEQ ID NO: 8: (i) CHARACTERISTICS OF THE SEQUENCE: (A) LENGTH: 19 Base pairs (B) TYPE: Nucleotide (C) TYPE OF HEBRA: individual (D) TOPOLOGY: linear (ii) ) TYPE OF MOLECULE: synthetic DNA (iii) HYPOTHETIC: NO (xi) DESCRIPTION OF THE SEQUENCE: SEQ ID NO: 8: GGCCCCCATA TC l l'CC 19 (2) INFORMATION FOR SEQ ID NO: 9: (i) CHARACTERISTICS OF THE SEQUENCE: (A) LENGTH: 1296 Pairs of bases (B) TYPE: Nucleotide (C) TYPE OF HEBRA: double (D) TOPOLOGY: linear (ii) ) TYPE OF MOLECULE: cDNA (iii) HYPOTHETIC: NO (vi) ORIGINAL SOURCE: (A) ORGANISM: Solanum tuberosum (xi) DESCRIPTION OF SEQUENCE: SEQ ID NO: ATG-TAGGAT CCTITGGTTC TTCA_X_ TCT CAATCTCACC CTXATCATGA _X_AAGAATCT 60 _X_TGATCATC A_CAACGGCG TAGATTCACC GCTACTACTA CAACTATCAC CACCACCACC 120 P1593 / 99MX ACAACGACCT (ACCAGCTAT .T-AAATCCGC CAGCTACTCA TTAGCTGTGC GGAG-TGATT 180 TCODGGTCCG AT ETCTCGGC CGCGAAAAGA CTCCTTACCA TATTATCAAC TAACTOTTCT 240 CCTTTTGGTG ATICAACIGA ACO_TTAGTC C? _X_AGTTTA C_n3CGCACT T_rCCTTCGT 300 CTCAACCGCT ATATATCGTC AACCACCAAT CATTTCATGA CACCTr_TTGA AACAACTCCA 360 ACTGATTCTT CATC-TCGTT GGCATCGTCA _r_A- TAGCTC TAATTCAATC ATCATATCAT 420 -X.TCTAAATC AAG-II? CCCC TGTTATAAGG TTTACTCAAT TAACCGCTAA TC? AGCGATr 480 TTAGAAGOGA TTAAOGGTAA _r_A.r_AA.GCA ATCCACATCG TTGATTTCGA CATTAATCAC 540 GGGGTTCAAT GGCCACCGTT AATGCAAGCA CTAGCTGATC GTGACCCTGC TCCTACTC.T 600 CX.AATCACCG CTACTGGAAA .rACCTTGAT ACO_TIO_TA GAACAGG_r_A Ta__TTAGCT 660 AAAT-TCCTC ACTCATIA.GG G-TGAGATTT CAATTCCATC CTrTITATAT 03_CAATAAT 720 AAC < _GCGATC ACX_G_r_AAGA _rXT_r_TATT A? TTCCTOCA TIGTACTTCT CCCTGATGAA 780 ACO_TAGCTA TTJAACTGTGT TITCTATCTC CACCDCCTTT TAAAAGACCG CGAAAAATTA 840 AGGAITI I TGCATAGGGT TAAGTCAATG AACCCTAAAA _ K_TTACAAT CGCGGAGAAG 900 GAAGCAAATC ATAACX? TCC T l'lTTl'lA CAAAGATTTA _Kr_AGC ^ CGTT GGATTATTAT 960 ACAGCTGTGT T_r_ATTCATT GGAAGCTACA TTGCCACCGG GTAGTCGTGA GAGGATGACA 1020 GTTGAACAAG _r_TG3TTTGG GAGAGAAA-T GTTGATATCG TGGQGATGGA AGGAGATAAA 1080 AGGAAAGAAA GACATGAAAG GITTAGATCA TGGGAAGTTA TGTTGAGGAG TK3TCGATTT 1140 AGTAATGTTG CTTTAAGCCC TITTCCATTA _r_ACAAGCTA AGC- CT1 -T C ^ AGACTACAT 1200 TATCCITCTG AAGGCTATCA ACTCX_GAGTT TCGAGTAATG CTTTC 1C1 AGG-TGGCAA 1260 AA-TIAACCTC T-TTCTCCAT CTCG-XJTTGG CGTTGA 1296 (2) INFORMATION FOR SEQ ID NO: 10: (i) CHARACTERISTICS OF THE SEQUENCE: (A) LENGTH: 431 amino acids (B) TYPE: amino acid (C) TYPE OF HEBRA: individual (D) TOPOLOGY: linear (ii) TYPE OF MOLECULE: Protein (vi) ORIGINAL SOURCE: (A) ORGANISM: Solanum tuberosum (xi) DESCRIPTION OF THE SEQUENCE: SEQ ID NO: 10: P1593 / 99MX Met Leu Gly Being Phe Gly Being Ser Being Gln Ser His Pro His His 1 5 10 15 Asp Glu Glu Being Asp His His Gln Arg Arg Arg Phe Thr Wing Thr 20 25 30 Thr Thr Thr Thr Thr Thr Thr Thr Thr Ser Pro Wing He Gln 35 40 45 He Arg Gln Leu Leu He Ser Cys Wing Glu Leu He Ser Arg Ser Asp 50 55 60 Phe Be Wing Wing Lys Arg Leu Leu Thr He Leu Ser Thr Asn Ser Ser 65 70 75 80 Pro Phe Gly Asp Ser Thr Glu Arg Leu Val His Gln Phe Thr Arg Wing 85 90 95 Leu Ser Leu Arg Leu Asn Arg Tyr He Ser Ser Thr Thr Asn His Phe 100 105 110 Met Thr Pro Val Glu Thr Pro Thr Asp Ser Ser Ser Leu Pro 115 120 125 Ser Ser Leu Ala Leu He Gln Ser Ser Tyr His Ser Leu Asn Gln 130 135 140 Val Thr Pro Phe He Arg Phe Thr Gln Leu Thr Ala Asn Gln Ala He 145 150 155 160 Leu Glu Ala He Asn Gly Asn His Gln Ala He His He Val Asp Phe 165 170 175 Asp He Asn His Gly Val Gln Trp Pro Pro Leu Met Gln Ala Leu Wing 180 185 190 Asp Arg Tyr Pro Wing Pro Thr Leu Arg He Thr Gly Thr Gly Asn Asp 195 200 205 Leu Asp Thr Leu Arg Arg Thr Gly Asp Arg Leu Wing Lys Phe Ala His 210 215 220 Ser Leu Gly Leu Arg Phe Gln Phe His Pro Leu Tyr He Ala Asn Asn 225 230 235 240 Asn Arg Asp His Gly Glu Asp Pro Ser He He Ser Ser Be He Val Leu 245 250 255 Leu Pro Asp Glu Thr Leu Wing He Asn Cys Val Phe Tyr Leu His Arg 260 265 270 Leu Leu Lys Asp Arg Glu Lys Leu Arg He Phe Leu His Arg Val Lys 275 280 285 Ser Met Asn Pro Lys He Val Thr He Ala Glu Lys Glu Ala Asn His / 99MX 290 295 300 Asn His Pro Leu Phe Leu Gln Arg Phe He Glu Ala Leu Asp Tyr Tyr 305 310 315 320 Tfctr Wing Val Phe Asp Ser Leu Glu Wing Thr Leu Ero Pro Gly Ser Arg 325 330 335 Glu Arg Met Thr Val Glu Gln Val Trp Phe Gly Arg Glu He Val Asp 340 345 350 He Val Wing Met Glu Gly Asp Lys Arg Lys Glu Arg His Glu Arg Phe 355 360 365 Arg Ser Trp Glu Val Met Leu Arg Ser Cys Gly Phe Ser Asn Val Wing 370 375 380 Leu Ser Pro Phe Ala Leu Ser Gln Ala Lys Leu Leu Leu Arg Leu His 385 390 395 400 Tyr Pro Ser Glu Gly Tyr Gln Leu Gly Val Ser Ser Asn Ser Phe Phe 405 410 415 Leu Gly Trp Gln Asn Gln Pro Leu Phe Ser Be Ser Ser T_tp Arg 420 425 430 (2) INFORMATION FOR SEQ ID NO: 11: (i) CHARACTERISTICS OF THE SEQUENCE: (A) LENGTH: 20 Pairs of bases (B) TYPE: Nucleotide (C) TYPE OF HEBRA: individual (D) TOPOLOGY: linear (ii) ) TYPE OF MOLECULE: synthetic DNA (iii) HYPOTHETIC: NO (xi) DESCRIPTION OF THE SEQUENCE: SEQ ID NO 11 CARTGGCCNC CNYTNATGCA 20 (2) INFORMATION FOR SEQ ID NO: 12: (i) CHARACTERISTICS OF THE SEQUENCE: (A) LENGTH: 20 Pairs of bases (B) TYPE: Nucleotide (C) TYPE OF HEBRA: individual (D) TOPOLOGY: linear (ii) ) TYPE OF MOLECULE: synthetic DNA P1593 / 99MX (iii) HYPOTHETIC: NO (xi) DESCRIPTION OF THE SEQUENCE: SEQ ID NO: 12: TGRTTYTGCC ANC? SIARR ?? twenty (2) INFORMATION FOR SEQ ID NO: 13: (i) CHARACTERISTICS OF THE SEQUENCE: (A) LENGTH: 687 Pairs of bases (B) TYPE: Nucleotide (C) TYPE OF HEBRA: double (D) TOPOLOGY: linear (ii) ) TYPE OF MOLECULE: cDNA (iii) HI POTETHICAL: NO (vi) ORIGINAL SOURCE: (A) ORGANISM: Arabidopsis thaliana (xi) DESCRIPTION OF SEQUENCE: SEQ ID NO: 13: GAGAGGTCAT CAAACCCTAG CAGTCCACCT CCATCTCTCC GCATAACCGG ATCCGGTCGA 60 GATGTAACCG GATTAAACCG AACIGGAGAC CX3GTTAACCC GGTTCGCIGA CTC TTAGGT 120 CTCCAATICC AGJTTTCACAC GCTAGTGATC GTAGAAGAAG ATCICGCCGG ACr TJGCTA 180 CAGATCCGAT TGTTAGCTCT CTX.ACCCGTA (ZAAGGAGAGA (XATIGCCGT CAA_Iir_TGTT 240 CACITCCTCC ACAAAATATT TAACGACGAT GGAGATATGA TCGGTCACTT CTTGTCAGCG 300 ATCAAGAGCT TAAACTCTAG AATCGTTACA ATGGCAGAGA GAGAAGCTAA TX_A.TGGA.GAT 360 CACTCGTTCT TGAATAGATT CTCTGAGGCA G_X_GATCATT ACATCGCGAT CTITGATTCG 420 TTGGAAGCGA CGTTGCCGCC AAATAGCCGA GAGAGACTAA CCCTAGAGCA ACGGTGGTTC 480 GGTAAGGAGA TTTTGGATGT TG-X3GCGGCG GAAGAGAGGG AGAGAAAGCA AAGACATCGG 540 AGGTTTGAGA TTTGGGAAGA GATCATGAAG AGGTTp_GTT TCGTTAACGT TCCTATTGGA 600 AGCT-TGCIT TOTCTCAAGC TAAGC T IT 'C-TACACTTC A.T? TCCTTC AGAAGGTTAT 660 AATCTTCAGT TCCTTAACAA H T TG 687 (2) INFORMATION FOR SEQ ID NO: 14: (i) CHARACTERISTICS OF THE SEQUENCE: (A) LENGTH: 229 amino acids P1593 / 99MX (B) TYPE: amino acid (C) TYPE OF HEBRA: individual (D) TOPOLOGY: linear (ii) TYPE OF MOLECULE: Protein (vi) ORIGINAL SOURCE: (A) ORGANISM: Arabidopsis thaliana (xi) DESCRIPTION OF THE SEQUENCE: SEQ ID NO: 14 Glu Arg Being Ser Asn Pro Being Ser Pro Ero Pro Being Leu Arg He Thr 1 5 10 15 Gly Cys Gly Arg Asp Val Thr Gly Leu Asn Arg Thr Gly Asp Arg Leu 20 25 30 Thr Arg Phe Wing Asp Ser Leu Gly Leu Gln Phe Gln Phe His Thr Leu 35 40 45 Val He Val Glu Glu Asp Leu Ala Gly Leu Leu Leu Gln He Arg Leu 50 55 60 Leu Ala Leu Ser Wing Val Gln Gly Glu Thr He Wing Val Asn Cys Val 65 70 75 80 His Phe Leu His Lys He Phe Asn Asp Asp Gly Asp Met He Gly His 85 90 95 Phe Leu Ser Wing He Lys Ser Leu Asn Ser Arg He Val Thr Met Wing 1Q0 105 110 Glu Arg Glu Wing Asn His Gly Asp His Ser Phe Leu Asn Arg Phe Ser 115 120 125 Glu Ala Val Asp His Tyr Met Wing He Phe Asp Ser Leu Glu Ala Thr 130 135 140 Leu Pro Pro Asn Ser Arg Glu Arg Leu Thr Leu Glu Gln Arg Trp Phe 145 150 155 160 Gly Lys Glu He Leu Asp Val Val Ala Wing Glu Glu Thr Glu Arg Lys 165 170 175 Gln Arg His Arg Arg Phe Glu He Trp Glu Glu Met Met Lys Arg Phe 180 185 190 Gly Phe Val Asn Val Pro He Gly Ser Phe Ala Leu Ser Gln Ala Lys 195 200 205 Leu Leu Leu Arg Leu His Tyr Pro Ser Glu Gly and Asn Leu Gln Phe 210 215 220 Leu Asn Asn Ser Leu 225 pmíí / Q_QMV

Claims (17)

1. NOVELTY OF THE INVENTION Having described the present invention, it is considered as a novelty and, therefore, the content of the following CLAIMS is claimed as property; 1. A sequence in the nucleotides according to SEQ ID NO: 1, 9 or 13 which is responsible for the control of lateral sucker formation and / or formation of petals and / or formation of abscission zones, the fragment or derivative of the same or a sequence of nucleotides that hybridize with the nucleotide sequence according to SEQ ID NO: 1, 9 or 13, and which is responsible for the control of lateral sucker formation and / or formation of petals and / or formation of abscission zones. The nucleotide sequence according to claim 1, wherein the hybridization nucleotide sequence hybridizes to the nucleotide sequence according to SEQ ID NO: 1, 9 or 13 under severe conditions. 3. A nucleotide sequence as illustrated in SEQ ID NO: 1, 9 or 13. 4. A polypeptide having an amino acid sequence as illustrated in SEQ ID NO: 2, 10 or 14. 5. The vector comprising a nucleotide sequence according to any of claims 1 to 3. P1593 / 99MX 6. A transformed plant cell or tissue, characterized in that an expressible DNA sequence, responsible for the control of side shoot formation and / or formation of petals and / or formation of abscission zones, or fragment or derivative of it according to claim 1 or 2 is stably integrated into the genome of the cell of the plant or tissue of the plant. 7. A plant cell or tissue of the plant according to claim 6, which can be regenerated in a seed producing plant. 8. A method for the preparation of plants having controlled formation of side shoots and / or formation of petals and / or formation of abscission zones, comprising the integration of at least one expressible DNA sequence, responsible for the control of the formation of side shoots and / or formation of petals and / or formation of abscission zones or fragment or derivative thereof according to claim 1 or 2 in the genome of plant cells or plant tissues and regeneration of the resulting plant cells or tissues of plant resulting in the plants. The method according to claim 8, wherein for the integration a DNA sequence or fragment or derivative thereof is used which suppresses the formation of side shoots and / or formation of P1593 / 99MX petals and / or formation of abscission zones. The method according to claim 9, wherein the integrated DNA sequence or fragment or derivative thereof is expressed in an antisense orientation relative to the endogenous sequence responsible for control of lateral sucker formation and / or formation of petals and / or formation of abscission zones. The method according to claim 9, wherein the integrated DNA sequence or fragment or derivative thereof is expressed in a homosense orientation relative to the endogenous sequence responsible for control of lateral sucker formation and / or formation of petals and / or formation of abscission zones. The method according to claim 9, wherein the formation of side shoots and / or formation of petals and / or formation of abscission zones is suppressed by a ribozyme comprising the integrated DNA sequence or fragment derived therefrom. The method according to claim 9, wherein the DNA sequence in fragment or derivative thereof is integrated into the genomic region of the homologous endogenous gene by homologous recommendation. The method according to claim 8, wherein the integration of a DNA sequence or fragment or derivative thereof is used which improves the formation of side shoots and / or formation of P1593 / 99MX petals and / or formation of abscission zones. The method according to claim 14, wherein the integrated DNA sequence or fragment derived therefrom is expressed in a homosense orientation in a relationship to the endogenous sequence responsible for control of lateral sucker formation and / or formation of petals and / or formation of abscission zones. 16. The method according to any of claims 8 to 15, wherein a tomato plant, a rapeseed plant, a potato plant or an antirrhinum plant or the cell or tissue thereof is used. 17. A plant obtainable according to any of claims 8 to 16. 18. Varieties of seeds obtained from plants according to claim 17. P1593 / 99MX SUMMARY OF THE INVENTION Nucleotide sequences encoding polypeptides are described which are responsible for the control of side shoot formation and / or formation of petals and / or formation of abscission zones, as well as polypeptides and amino acid sequences encoded by the nucleotide sequence. Also described are plants that have a controlled formation of side shoots and / or controlled formation of petals and / or controlled formation of abscission zones, wherein the sequence of expressible DNA or fragment or, derivatives thereof, responsible for the formation of lateral sprouts and / or formation of petals and / or formation of abscission zones, is integrated in a stable manner in the genome of the plant cell or plant tissue. Additionally, methods are described for the production of plants having controlled lateral sucker formation and / or controlled formation of petals and / or controlled formation of abscission zones, wherein the expressible DNA sequence or, fragment or derivative thereof , responsible for the formation of side shoots and / or formation of petals and / or formation of abscission zones, is integrated in a stable manner in the genome of plant tissue cells and the resulting plant cells or tissues are regenerated to form plants. In addition, the invention relates to P1593 / 99MX plants and seed varieties of plants, which can be obtained according to the method of the invention. P1593 / 99MX