MXPA99007102A - Use of peroxygen compounds in the control of hairy wart disease - Google Patents

Abstract

A method of preventing growth of hairy warts and preventing hairy wart disease in dairy cattle by applying to a potentially infected area of the cattle, e.g. hooves, a solution comprising a peroxycarboxylic acid, e.g. of C2-C18 carbon atoms or mixtures thereof, particularly peracetic acid, is described.

Description

USE OF PERCARBOXYLLIC ACIDS IN THE CONTROL OF VERRUGA VELLOSA DISEASE FIELD OF THE INVENTION The invention relates to the prophylactic treatment of leg diseases in dairy cattle. More specifically, the invention relates to the use of percarboxylic acids in the control of organisms, which cause the hairy wart disease in dairy cattle.

BACKGROUND OF THE INVENTION Warty wart disease, also called Papillomatous Digital Dermatitis (PDD), Infectious Bovine Interdigital Dermatitis (IDD), Digital Dermatitis (DD) or Stable Wasp of the Para (SFR) is an infectious disease transmitted between animals. The effects of the disease are lameness, weight loss, state decline in general; in the case of dairy cattle, there is a loss of milk production and the resulting economic loss of the dairy farmer. Intervention surgery may be required to protect the animal's life. It is believed that the disease is caused by Fusobacterium mecrophorum, Dichelobacter nodosus and Bacteroides melaninogenicus. All are anaerobic spirochetes, which live in the intestinal tract, and It is reported that they live in manure / dirt for up to ten months. Current treatments include leg baths containing copper sulfate, zinc sulfate, formaldehyde, tetracycline and mixtures containing sodium hydroxide and sodium hypochlorite. It has been reported that only tetracycline is effective and generally when combined with some surgical techniques including a bandage of the infected area that has been treated with tetracycline. Thus, there is a need for a product that could be more effective in controlling hairy wart disease than currently available products, especially since the disease is becoming one of the largest emissions faced by milk producers due to reports of the disease that extends through dairy herds in the United States. The use of percarboxylic acids in antimicrobial compositions has been reported, for example, as sterilizing agents, antimicrobial agents, and disinfectants. Particularly, peroxyacetic acid has been used in poultry farm applications as a general disinfectant, where the control of bacteria and viruses is a critical factor. The use of peroxyacetic acid directly in animals to treat infectious diseases has not been reported.

COMPENDIUM OF THE INVENTION The invention relates to the novel use of peroxycarboxylic acid compounds in hairy warts and / or related leg diseases in dairy cattle. It has been found that a peroxycarboxylic acid product included in the present invention is effective in preventive programs, ie, the prophylactic treatment of animals. The preventive program, that is, the prophylactic treatment, employs a foot bath where all the animals of the herd are exposed to the solution on a daily basis. Alternatively, herd animals can be treated on a daily basis by spraying or foaming the product on potentially infected areas. The product employs a prophylactically effective amount of peroxycarboxylic acid. The recommended doses use 14.8-290.6 ml, preferably 14.8-88.7 ml per liter of diluent, for example, water. Most preferably, 29.6 ml per 6.6 liters are used. As an alternative, direct treatment of the infected animals involves spraying a therapeutically effective amount of peroxycarboxylic acid in a diluent. The recommended doses include 29.6-290.6 ml per liter of diluent. A resulting solution is directly sprayed on the infected areas for a period of 3-10 days. Total resistance concentrations can be used in severe cases.

Accordingly, the present invention in its first aspect is a method for controlling hairy wart disease in dairy cattle comprising daily applying to said cattle a prophylactically effective amount of an antimicrobial concentrate composition comprising a peroxycarboxylic acid and a diluent.

DETAILED DESCRIPTION The process to prevent hairy wart disease in dairy cattle is done by applying a solution containing peroxycarboxylic acid (s). The peroxycarboxylic acid (s) is included in an antimicrobial concentrate composition, which may also contain the corresponding precursor carboxylic acid and hydrogen peroxide and the remainder being water. This concentrate before application to dairy cattle is diluted with a diluent, preferably water, which is sprayed, foamed or otherwise applied to the hooves of dairy cattle or other potentially infected areas of dairy cattle for prevention. An alternative preventive method is to use the solution in a leg bath for the dairy herd.

Composition of Antimicrobial Concentrate The concentrate composition includes an equilibrium mixture of peroxycarboxylic acid (s), its carboxylic acid (s) corresponding, hydrogen peroxide and water. The concentrate may also include other ingredients such as stabilizers, couplers, etc., as mentioned below. Among the above constituents in the antimicrobial concentrate composition, the invention comprises a carboxylic acid. Generally, the carboxylic acids have the formula R-COOH, where R can represent any number of different groups, including aliphatic groups, alicyclic groups, heterocyclic groups, all these may be saturated or unsaturated, as well as substituted or unsubstituted. The carboxylic acids also occur having one, two, three, or more carboxyl groups. The carboxylic acids tend to acidify aqueous compositions. In acid systems, they may also exhibit antimicrobial activity. The constituent, peroxycarboxylic acid, within the present invention, functions as the antimicrobial agent. In addition, the peroxycarboxylic acid constituent, within the invention, as well as the carboxylic acid of origin, maintain the composition at an acidic pH. Peroxycarboxylic acids generally have the formula, R (C03H) n, wherein R may represent any number of different groups, including aliphatic groups, alicyclic groups, aromatic groups, heterocyclic groups, all of these may be saturated or unsaturated, as well as substituted or unsubstituted From this way, R may be, for example, an alkyl, arylalkyl, cycloalkyl, aromatic or heterocyclic group, and n is one, two, or three, and termed by placing the prefix of per or peroxy to the acid of origin. Although peroxycarboxylic acids are less chemically stable than their corresponding carboxylic acids, their stability generally increases with increasing molecular weight. Generally, the decomposition of these acids can proceed by radical and nonradical trajectories, through photodecomposition or radical-induced decomposition, through hydrolysis or dissociation or through the action of metal ions or complexes. Peroxycarboxylic acids can be made through the direct action of acid-catalyzed equilibrium of 10-98% by weight of hydrogen peroxide with carboxylic acid, through self-oxidation or perhydrolysis of aldehydes, or from carboxylic acid halides, or carboxylic anhydrides with hydrogen, sodium peroxide, or other in situ sources of hydrogen peroxide. Peroxycarboxylic acids useful in this invention include peroxycarboxylic acids of 2 to 18 carbon atoms such as, for example, peracetic acid, perpropionic acid, perbutyric acid, perhexanoic acid, perheptanoic acid, peroctanoic acid, pernonanoic acid, perdecanoic acid, perbenzoic acid, perglycolic acid, perglutaric acid, persuccinic acid, perlactic acid, percyclic acid, perlauric acid, peradipic acid, permálico acid, perfumárico acid, pertartárico, or mixtures thereof. It has been found that these peroxycarboxylic acids provide a good antimicrobial action with good stability in aqueous solutions. In addition to peracetic acid, peroctanoic and perdecanoic, particularly preferred peroxycarboxylic acids include perpropionic, perbutyric, perglycolic, perlactic, and percyclic acids. The antimicrobial concentrate composition can also use a mixture of peroxycarboxylic acids. The preferred is a combination of peracetic acid with other percarboxylic acids, preferably those referred to above and in particular, peroctanoic acid. It has been found that this combination of peroxycarboxylic acids provides preferred antimicrobial efficacy and stability. In general, the ratio of peroctanoic acid to peracetic acid can vary from about 1: 1 to 1: 9. In its highly preferred mode, the antimicrobial concentrate composition of the invention utilizes peracetic acid. Peracetic acid is a peroxycarboxylic acid that has the formula: CH3COOOH Generally, peracetic acid is a liquid that has a pungent odor at very high concentrations and is freely soluble in water, alcohol, ether and sulfuric acid. Peracetic acid can be prepared through any number of known means by those skilled in the art, including preparation from acetaldehyde and oxygen in the presence of cobalt acetate. A 50% solution of peracetic acid can be obtained by combining acetic anhydride, hydrogen peroxide and sulfuric acid. Other methods of formulation of peracetic acid include those described in the patent of E.U.A. No. 2,833,813, which is incorporated herein by reference. Other methods for the formulation of peroxycarboxylic acids within the present invention include those described in the U.S. Patents. Nos. 4,051,058, 4,051,059, 5,200,189, 5,314,687 and 5,437,868, which are incorporated herein by reference.

Hydrogen Peroxide The antimicrobial concentrate composition of the invention may also comprise a hydrogen peroxide constituent. The hydrogen peroxide in combination with the peroxycarboxylic acid provides a surprising level of antimicrobial action against microorganisms. In addition, hydrogen peroxide can provide an effervescent action, which can irrigate any surface to which it is applied. Hydrogen peroxide works with a mechanical washing action once it is applied, which also cleans the surface of the application area. An additional advantage of hydrogen peroxide is the food compatibility of this composition after use and decomposition. For example, combinations of peracetic acid and peroxide hydrogen result in acetic acid, water, and oxygen after the decomposition of all, which are compatible with food products. Although oxidizing agents can be used, hydrogen peroxide is generally preferred for a number of reasons. After the application of H202 / germicidal agent, peracetic acid, the residue left merely comprises water and an acid constituent. The deposition of these products on the surface of the application area, such as limbs and hooves of dairy cattle, will not give rise to adverse effects. Hydrogen peroxide (H202) has a molecular weight of 34,014 and is a colorless, transparent, weakly acidic liquid. The four atoms are covalently linked in a non-polar H-O-O-H structure. In general, hydrogen peroxide has a melting point of -0.41 ° C, a boiling point of 150.2 ° C, a density at 25 ° C of 1.4425 grams per cm3, and a viscosity of 1245 centipoise at 20 ° C. Generally, the concentration of hydrogen peroxide within the composition used in the process of the invention ranges from about 1% by weight to about 50% by weight, preferably from about 3% by weight to about 40% by weight, and very preferably from about 5% by weight to about 30% by weight. These concentrations of hydrogen peroxide can be elevated or decreased, but still remain within the scope of the invention.

Auxiliaries The antimicrobial composition of the invention can also comprise any number of auxiliaries. Specifically, the composition of the invention may comprise stabilizing agents or wetting agents among any number of constituents, which may be added to the composition. The stabilizing agents can be added to the composition of the invention to stabilize the peracid and the hydrogen peroxide. The chelating or sequestering agents generally useful as stabilizing agents in the invention include chelating agents of the alkyl diaminopiacetic acid type such as EDTA (ethylenediaminetetraacetic acid and its salts, for example the tetrasodium salt), stabilizing agents of the acrylic acid type, and polyacrylic, phosphonic and diphosphonic acids, phosphonate type chelating agents, among others. Preferred sequestering agents include phosphonic acids and phosphonate salts including 1-hydroxyethylidene-1,1-diphosphonic acid (CH3C (P03H2) 2? H), amino [tri (methylene phosphonic)] acid, 2-fosfen-butan- 1, 2,4-tricarboxylic acid, as well as the alkali metal salts, ammonium salts, or alkyl and amine salts, such as mono, di or tri-ethanolamine salts. The stabilizing agent is used in a concentration ranging from about 0 wt% to about 20 wt% of the composition, preferably of about 0.1% by weight to about 10% by weight of the composition, and most preferably from about 0.2% by weight to 5% by weight of the composition. Also useful in the composition of the invention are the wetting agents. The wetting agents function to increase the penetration activity of the antimicrobial composition of the invention and include any of those constituents known in the art to reduce the surface energy of the composition of the invention. In addition, the wetting agents can help to solubilize the fatty and peroxy fatty acids, improve the foaming and increase the storage stability of the concentrate composition. As a wetting agent, the compositions of the invention may include a hydrotrope coupling agent of surfactant or solubilizer which allows the mixing of both fatty acids and short chain peroxy fatty acids in aqueous liquids. Functionally speaking, suitable couplers that can be employed are non-toxic and retain the fatty acid and the fatty peroxy acid in aqueous solution through any use to which the solution is exposed. A preferred class of wetting or hydrotropic coupling or solubilizing agent, which can be used in the present invention are anionic surfactants of the sulphonate and sulfate type, such as alkylbenzenesulfonates having 6-18 atoms of carbon in the alkyl, alkyl sulfates and / or alkanesulphonates (each having 8-22 carbon atoms in the alkyl or alkane group). The alkylbenzene sulphonates which can be used are preferably those which contain an alkyl radical of 6-18 carbon atoms, preferably 8-15 carbon atoms. Instead of the alkylbenzene sulfonates, it is also possible to use alkyl sulphates or alkanols with an alkyl or alkane radical of the chain length of 8-18 carbon atoms. If desired, mixtures of the anionic surfactants mentioned above can also be used. Nonionic surfactants may also be useful in the present invention, for example, those comprising portions of ethylene oxide, portions of propylene oxide, as well as mixtures thereof, and portions of ethylene oxide-propylene oxide in his heterical or block formation. Further useful in the present invention are the nonionic surfactants comprising alkyl ethylene oxide compounds, alkyl propylene oxide compounds, as well as mixtures thereof, and alkyl ethylene oxide-propylene oxide compounds, wherein the The portion of ethylene oxide and propylene oxide is in either heteric or block formation. Also useful in the present invention are the nonionic surfactants having any mixture or combination of ethylene oxide-propylene oxide portions linked to the alkyl chain, wherein the ethylene oxide and propylene oxide portions may be in any random pattern or in order and of any specific length. The nonionic surfactants useful in the present invention may also comprise randomized sections of ethylene oxide, block or heterylene propylene oxide, or ethylene oxide-propylene oxide. In general, the concentration of surfactant used in the invention may vary from about 0 wt% to about 5 wt% of the composition, preferably from about 0 wt% to about 2 wt% of the concentrate composition and most preferably about 0% by weight to 1% by weight of the composition. The invention may also contain any number of other constituents as needed by the application, which are known to those skilled in the art and which may facilitate the activity of the present invention. The concentrate composition used in the invention may comprise: C om position (P-%) Useful Of Favorite job Peroxycarboxylic acid 2-25 2-20 4-20 H202 1-45 5-35 7-30 Carboxylic acid 1-70 3-55 5-45 Surfactant 0-20 0-10 0-5 Chelating agent 0-20 0-10 0-5 Water Rest Rest Rest Generation of Peroxy Acids The process of the invention can also be initiated through the use of peroxy acid concentrate compositions. In such a case, the peroxycarboxylic acid can be either naturally generated or through the combination of a hydrogen peroxide concentrate together with a carboxylic acid concentrate in view of the use of said process, which is described by Lokkesmoe et al. from the USA No. 5,122,538, issued June 6, 1992, which is incorporated herein by reference. In such a case, the composition can be formed from a hydrogen peroxide concentrate comprising varying levels of hydrogen peroxide and stabilizer as shown in the following table.

Concentration (P-%) Constituent Useful Of Preferred work Hydrogen peroxide 5-70 15-70 25-60 Stabilizer 0-10 0-5 0.1-3 H20 20-95 25-85 37-75 When combined with a carboxylic acid, the two concentrates result in a peroxycarboxylic acid. In general, the carboxylic acid concentrate comprises a carboxylic acid in water, as shown in the table below.

Concentration (P-%) Constituent Useful Preferred work Carboxylic acid 50-100 65-100 80-100 Water 0-50 0-35 0-20 Control of Hairy Wart Disease Direct treatment of existing warts in dairy cattle can be accomplished by administering through a spray a concentration of approximately 28.35-283.5 grams of an antimicrobial concentrate composition, as previously described by 3,784 liters of diluent, for example, of water, on the affected areas once a day or in each slurry for periods of 3-10 days. Alternatively, and especially for severe cases, the antimicrobial concentrate composition may be sprayed, foamed or otherwise applied to the infected area without a diluent. This procedure can then be followed by additional treatments in each slurry of about 14,175-85.05 grams, preferably 42.52 grams, of concentrate per 3,784 liters of diluent until the wart has been eliminated. The maintenance / prevention or prophylactic treatment can be performed either by spraying or using leg baths at concentrations of approximately 14,175-283.5 grams of the antimicrobial concentrate composition per liter of diluent. The preferred dose is 14.8-88.7 ml per liter; very preferably it is 44.4 ml per liter. When footbaths are used, they are recharged when the peroxycarboxylic acid runs out due to the introduction of significant dirt (organic and / or inorganic). The leg baths are kept complete for all grouts during the day. Spray applications are performed once a day on each slurry depending on the severity of the problem. Using the concentrate of the peroxycarboxylic acid formula described in Work Example 1, the concentration of active POAA in use concentrations is as follows: ml of product per liter of water Concentration of POAA (ppm) 14.8 254 28.3 508 44.4 762 88.7 1524 290.6 5080 without dilution 58,000 using product specific gravity (concentrate) = 1.12 ppm POAA = (ml / liter) x 1.12 x 0.058 x 106 128 For Spraying Warts Conc. Useful POAA 250-60,000 ppm Preferred 500-60,000 ppm Highly preferred 1,500-5,000 ppm It has been found that the above methods are effective in preventive programs for this disease. Thus, for example, the use of an antimicrobial concentrate composition containing 27.5% by weight of hydrogen peroxide and 5.8% by weight of peroxyacetic acid diluted with water to 0.5% by weight resulted in a percentage reduction of > 99.778% of bacteria against Dichelobacter (Bacteroides) nodosus and > 99.999% reduction of Fusobacterium necrophorum when tested in vitro. These bacteria are attributed to cause hairy wart disease.

WORK EXAMPLES The invention will now be described in more detail with reference to the following examples. The only appropriate construction of these examples is as non-limiting illustrative examples showing various formulations, stabilities and applications of the invention.

WORK EXAMPLE 1 A solution for supplying the peroxyacetic acid concentrate (or "POAA") was prepared for use in the tests described / containing the following components at the equilibrium concentration.

Component% / P eso Peroxyacetic acid 5.8 Hydrogen peroxide 27 .5 Acetic acid 8. 0 HEDPa 0. 9 LASB 1. 0 H20 Rest a ... HEDP is 1-hydroxyethylidene-1,1-diphosphonic acid (stabilizer) (Monsato-Dequest® 2010) b ... LAS is linear alkylbenzenesulfonate (anionic surfactant) The following test was performed in vitro using the POAA concentrate.

TEST ORGANISM: CULTURE MEDIA: Dichelobacter (Bacteroides) nodosus Tryptic soy agar with 5% (ATCC 27521) of blood Fusobacterium necrophorum (ATCC Tryptic Soy Agar with 5% 27852) of blood The microorganisms used in this study were obtained from American Type Culture Collection, Rockville, Maryland.

SUBCULTORY MEDIUM: Tryptic soy agar with 5% blood agar plate NEUTRALIZER OF START: 0.5% sodium thiosulfate EXPOSURE TIMES: 15 seconds TEMPERATURE OF EXPOSURE: 25 ° C METHOD: Official methods of analysis of AOAC, 15th edition, 1990.

Dilution of use of Germicide The POAA concentrate was prepared according to the directions for the intended use of the product. A 0.5% solution was made using 2.5 ml of POAA concentrate and 497.5 ml of sterile deionized water. The product was in solution and was used the day it was prepared.

Bacterial cultures Three (3) blood agar plates were inoculated with Fusobacterium necrophorum and 5 blood agar plates were inoculated with Dichelobacter (Bacteroides) nodosus. The blood agar plates were incubated in an anaerobic atmosphere for 4 days at 35-37 ° C. Bacterial growth was washed from each blood agar plate using 2 ml of phosphate pH regulator dilution water. For each organism, the growth suspension was aspirated and combined together in a sterile container. The combined suspension of each organism was swirled and used for testing.

Procedure 99 ml of the product was added to the concentration that will be tested to each of the two Erlenmeyer flasks, wide mouth, 250 ml, and placed in a water bath at 25 ± 2 ° C (> _ 20 minutes). Similar flasks were prepared containing 99 ml of sterile phosphate pH regulator dilution water which will be used for the control of "initial numbers". 1 ml of the culture suspension was added to each flask, as follows: a) The flask was rotated and stopped just before adding the suspension, which created a sufficient residual movement of liquid to avoid the combination of the suspension in the point of contact with the test water. b) The suspension was added halfway between the center and the edge of the surface with the tip of a pipette slightly submerged in the test solution. (The pipette on the neck or side of the flask was avoided during the addition).

Inoculation of the Subculture Medium Test Samples 1 ml of the exposed culture was added to 9 ml of neutralizer templates exactly 15 seconds after the addition of the suspension. After mixing, four aliquots of 1.0 ml and four aliquots of 0.1 ml were transferred to individual blood agar plates using the extended plate technique. The recovery plates of Fusobacterium necrophorum were incubated for 3 days at 35-37 ° C before enumerating the survivors The recovery plates of Dichelobacter (Bacteroides) nodosus were inoculated for 6 days at 35-37 ° C before the enumeration of the survivors.

Control Number 1 ml of the "initial number" flask culture was added to 99 ml of phosphate pH regulator dilution water (Dilution 1). After thorough mixing, 1 ml of Dilution 1 was transferred to 99 ml of phosphate pH regulator dilution water (Dilution 2). A third dilution was made using 1.0 ml of Dilution 2 in 99 ml of phosphate pH regulator dilution water (Dilution 3). Four aliquots of 1 ml and four aliquots of 0.1 ml of Dilution 3 were transferred to individual Petri dishes. 15 to 20 μl of blood agar was added to each plate and the agar was cooled to solidify. The plates were inverted and incubated as in the test. The initial suspension was enumerated to determine the cfu / ml number.

Controls Fusobacterium necrophorum Dichelobacter (Bacteroides) nodosus a. Neutralizer control No growth No growth b. Phosphate pH regulator dilution water No growth No growth c. Neutralization Confirmation (NC) 1 ml and 0.1 ml aliquots of the neutralization control samples (9 ml neutralizer template tube inoculated with 1 ml of the exposed culture) were transferred to the blood agar plates. 1 ml of the suspension of the test organism was added to the NC blood agar plate, as well as to a sterile blood agar control plate. The blood agar plates were incubated as in the test. Blood agar plates were examined for the presence of colonies on the surface of the agar and compared with the colonies on the surface of the blood agar control plate.

Dichelobacter (Bacteroides) nodosus: Neutralization inoculum: 1.0 ml of Dichelobacter (Bacteroides) nodosus suspension containing TNTC CFU / ml.

Neutralization controls showed growth, eliminating bacteriostasis as a cause of lack of growth in the test system.

Fusobacterium necrophorum Neutralization inoculum: 1.0 ml of Fusobacterium necrophorum suspension containing TNTC CFU / ml.

Neutralization controls showed growth, eliminating bacteriostasis as a cause of lack of growth in the test system. TNTC = Too many numbers to count BAP = blood agar plate Test Results: after a contact time of 15 seconds Dichelobacter (Bacteroides) nodosus No control treatment 4.5 x 103 cfu / ml 0.5% Product (290 ppm POAA) < 1 cfu / ml% Reduction > 99.978% Fusobacterium necrophorum Without control treatment 28.3 x 106 cfu / ml 0.5% Product (290 ppm POAA) < 1 cfu / ml% Reduction > 99.999% cfu / ml = colony forming units per milliliter Notes: 1) The results are the average of two groups of plates in quadruplicate. In all cases less than 1 cfu / ml was found in the samples tested. 2) Product used: Work Example 1 When diluted to 0.5%, concentration of POAA = 290 ppm in use solution.

Calculations Calculate the% reduction. 1 - test # cfu X 100 =% reduction # cfu control The above specification, examples and data provide a complete description of the manufacture and use of the composition of the invention. Since many embodiments of the invention can be made without departing from the spirit and scope of the invention, the invention resides in the appended claims.

Claims (12)

1. A method for controlling hairy wart disease in dairy cattle, comprising daily applying to said cattle a prophylactically effective amount of an antimicrobial concentrate composition comprising a peroxycarboxylic acid and a diluent.

2. The method according to claim 1, wherein the peroxycarboxylic acid comprises peracetic acid.

3. The method according to claim 1, wherein said peroxycarboxylic acid comprises a percarboxylic acid of 2 to 18 carbon atoms or a mixture thereof.

4. The method according to claim 1, wherein said peroxycarboxylic acid is selected from the group consisting of peracetic, perpropionic, perbutyric, perglycolic, perláctico, percítrico, perlaurico, peradípico, perhexanoico, perheptanoico, peroctanoico, pernonanoico, perdecanoico acid. , persuccinic, permálico, perfumárico, pertartárico, perglutárico, perbenzoico, and mixtures of the same.

5. The method according to claim 1, wherein said antimicrobial concentrate composition comprises: about 2-25% by weight of peroxycarboxylic acid; about 1-45% by weight of hydrogen peroxide; about 1-70% by weight of a corresponding carboxylic acid, and the remainder being water.

6. - The method according to claim 5, wherein said antimicrobial concentrate composition further comprises a chelating agent.

7. The method according to claim 5, wherein said antimicrobial concentrate composition further comprises a surfactant.

8. The method according to claim 7, wherein said surfactant is an anionic surfactant.

9. The method according to claim 1, wherein a daily dose of about 14.8-290.6 ml per liter of said diluent antimicrobial concentrate composition is applied.

10. The method according to claim 9, wherein said daily dose is applied to cattle in a leg bath.

11. The method according to claim 9, wherein said daily dose is applied through automatic or manual spraying on a potentially infected area of said cattle.

12. The method according to claim 9, wherein said daily dose is applied through foaming on a potentially infected area of said cattle.