MXPA97009739A - Method to improve health ani - Google Patents

Abstract

The present invention relates to a method for improving animal health including selecting, at least one species of L. reuteri, characterized by the production of hydroxypropionaldehyde, and using the selected species (s) together with the antibiotic, gentamicin, as an animal treatment, for example, for newly hatched poultry

Description

METHOD TO IMPROVE SALU D ANI MAL FIELD OF THE INVENTION This invention relates to methods for using synergistic effects between antibiotics and probiotics to improve animal health.

Description of the Related Art Microbial contamination of animals, which are very susceptible to microbial pathogens, often leads to increased animal morbidity and disease. In commercial animal growth operations, where animals may be tight in facilities where other animals have been previously reared, the possibility of such contamination is often greater. This is particularly true in the poultry industry. Early chick mortality (ECM) is often associated with microbial exposure during incubation, hatching and processing. Pathogenic microorganisms commonly reside in and within the egg shell, and there is widespread dissemination of pathogens born from the egg during hatching. Researchers have tested numerous antimicrobial substances for years to see if they can improve animal mortality. Numerous probiotic species have also been proven for their effectiveness in improving animal health. Many of these tests have used several species of Lactobacillaceae, species such as Lactobacillus and Streptococcus. Most of these tests have shown limited utility and practicability in improving cases.

The work of Dobrogosz and Lindgren, and their collaborators in the mid-1 980s, which has been the target of a number of publications, however, showed that Lactobacillus reuteri species are unique in producing an identified antibiotic substance as ß-hid roxipropionaldeh ído. (See for example, Dobrogosz, WJ, IA Houses, Pagano, GA, Talarico, TL, Sjoberg, B and M Karlsson, Lactobacillus reuteri and the enteric Microbiota, in Requlator and Protective Role of the Normal Microflora, Gustafsen Symposium, McMillan Ltd , 1989, pages 283-292, and Dobrogosz, WJ and SE Lindg ren, Antibiotic Reuterin, International Application Published under the Patent Cooperation Treaty (PCT), PCT / US88 / 01 423, published on November 3, 1 988). In addition, L. reute ri was shown to be effective in inhibiting Salmonella, to increase gastrointestinal "villi" growth, by increasing the animal's weight gain, and to improve mortality. M ovo and atomization techniques have been shown to be useful in providing Lactobacillus reuteri cells to poultry to reduce ECM, as an alternative to the use of traditional antibiotics. Although several previous treatments have resulted in improved mortality, a real need has continued to reduce the number of animal deaths even more. Therefore, an objective of this invention is to provide a method for improving animal health, particularly the mortality of poultry, such as chickens and turkeys. Other objects and advantages will be more fully apparent from the following description and appended claims.

SUMMARY OF THE INVENTION The method of the invention for improving animal health, for example, for newly hatched poultry, includes the steps of selecting at least one species of L. reuteri, characterized by the production of β-hydroxypropionaldehyde , and use the selected species (s) together with the antibiotic, gentamicin, as a treatment.

Other aspects and features of the invention will be more fully apparent from the following description and appended claims.

BRIEF DESCRIPTION OF THE DIAMETERS PCI Figure 1 is a graph that shows the mortality of young chickens over time for the five treatments discussed in Example 1. The treatment (1) is shown by empty squares, the treatment (2) is shown by half full rhombuses, the treatment (3) is shown by empty circles, the treatment (4) is shown by empty triangles, and the treatment (5) is shown. ) is stamped by empty rhombuses.

DETAILED DESCRIPTION OF THE INVENTION AND PREFERAL MODALITIES OF THE MIS MA The present invention is a method for improving animal health, comprising: (a) selecting a species of Lactobacillus reuteri, which is characterized by producing β-hydroxypropionaldehyde under anaerobic conditions in the presence of glycerol or glyceraldehyde; (b) administering cells of said spice to said animals; and (c) treating said animals with gentamicin. In preferred embodiments, the cells are administered by spraying the animals daily, and / or by feeding the animals with a feed containing L. reuteri. In addition, the administration of. reuteri may include injection into the egg before breaking the shell, or spraying the eggs before breaking the shell. The invention shows in the present the synergistic effect of establishing L. reuteri in the animal in an amount sufficient to colonize the gastrointestinal tract of said animal, and treat the animal with gentamicin. For a particular animal system, Lactobacillus reuteri capable of colonizing the gastrointestinal tract of animals can be obtained from collections of seeds or from natural isolates from healthy animals. The methods for such isolation are explained in detail in the aforementioned PCT application by Dobrogosz et al. If the amount of inoculum of Lactobacillus reuteri for the particular animal system and the type of treatment is not known for that system, preliminary routine experimentation can be performed to determine the amount sufficient to result in colonization, using the method of coverage reported in the PCT application of Dobrogosz et al. To determine the presence and number of Lactobacillus reuteri. Typically, an amount of 1 03-1 04 per animal, administered once, or for multiple days, was found sufficient to colonize the gastrointestinal tract of the animal. The use of additional L. reuteri cells, as in the example herein, provides added assurance of rapid, substantial colonization. Similarly, using more than one type of L. reuteri treatment optimizes colonization as treatment does early in the animal's life. The features and advantages of the present invention will be more clearly understood by reference to the following example, which should not be considered as limiting the invention.

EXAMPLE I Treatment with L. reuteri and Gentamicin The eggs, which have been incubated for 1 8 days, are placed in two incubator consoles. The eggs in a console are sprayed with water 24 hours before the eggs are ready to break the shell, and the eggs in the other console are sprayed with Lactobacillus reuteri cells 24 hours before breaking the shell. In the experiment for which the results are given below, 0.1 ml / egg was sprayed at a level of 1 07 cells per ml. The L. reuteri species (s) used can be any species that colonizes the animals to be treated, which in the example below are chickens. The species of L. reuteri used in the example is species number 1 1284, ATCC No. 551 48, deposited under the Budapest Treaty with the American Type Cultu re Collection, Rockville, Maryland, on June 29, 1 991. Immediately after hatching, the following treatments were the following: (1) absolute control - L. reuteri was not added. nor E. enteropathogenic coli, nor gentamicin (30 birds hatched from eggs previously sprayed with water); (2) control of E. coli - 1 04 CFU of enteropathogenic E. coli were primed in each of the 30 birds hatched from eggs, which were previously sprayed with water; (3) E. coli plus gentamicin - E. coli as in the treatment (2) plus 0.2 mg of its gentamicin sulfate administered by injection of its skin using standard techniques in each of the 30 birds hatched from eggs, which were previously sprayed with water; (4) E. coli plus L. reuteri - E. coli as in treatment (2) with 30 egg birds sprayed with L. reuteri before hatching, plus feed with GAIAfeed R, containing approximately 1 05 CFU per gram of reuteri, as 2% of the feeding of the birds hatched during the 1st 1 day after birth; and (5) E. coli and L. reuteri as in the treatment (4), and gentamicin sulfate as in the treatment (3) with 30 birds hatched from eggs sprayed with L. reuteri before breaking the shell.

After the previous treatment, the chickens are transferred to Petersime incubators. Each treatment is placed in a separate isolation room but identically controlled. Mortality is determined daily, and body weights are determined in 21 days. L. reuteri and E. coli cecal were determined by breaking the shell and at the end of the experiment to assure that there was no cross contamination. The typical results of mortality and body weights on day 22 are shown in Table 1.

Table 1 TREATMENT BODY WEIGHT MORTALITY (1) Absolute control 842b 1 .42b (2) Only E. coli 803b 9.52§ (3) E. coll + gentamicin 81 9b 4.26b (4) E. coli + L. reuteri 874§ 3.56 b (5) E. coli + L. reuteri + 882 * 0.00b gentamicin a, b in a column, means if dissimilar, they are different (P <.01) * at 22 days of age Mortality results from day 0 to day 25 are shown in Figure 1 and show the differences between treatments over time. The results of this experiment show that spraying the eggs with L. reuteri and adding L. reuteri to the feed, in combination with the treatment with its gentamicin lf ato, provides excellent protection against mortality associated with E. coli. Body weight is significantly increased over the use of gentamicin alone, and mortality is essentially eliminated.

Further experimentation shows that similar results are obtained when eggs are injected with L. reuteri in addition to or in place of egg sprinkling. The optimal results are obtained as the establishment of L. reuteri is maximized in conjunction with the treatment of gentamicin sulfate.

PREFERRED MODE OF THE INVENTION The preferred invention is a method for improving animal health, comprising selecting a species of Lactobacillus reuteri, which is characterized by producing β-hydroxypropionaldehyde under anaerobic conditions in the presence of glycerol or glyceraldehyde, administering cell those of said species to said animals; and treating said animals with gentamicin. Preferably, the animals are poultry and the administration comprises feeding the animals with a feed containing approximately 1 05 CFU of Lactobacillus reuteri cells per g feed branch.

INDUSTRIAL APPLICABILITY The invention provides a method for making commercial animal growth operations more cost effective, particularly where animals are tightened in facilities where other animals have been previously reared, due to decreased animal mortality.

Although the invention has been described with reference to specific modalities thereof, it will be appreciated that numerous variations, modifications and modalities are possible, and according to this, all these variations, modifications and modalities will be considered to be within the spirit and scope of the invention. the invention. Particularly, also included within the invention is the use of L-reuteri treatment as is known in the art in any young animal, along with treatment with an antibiotic.

Claims (9)

1. REIVI NDICATIONS A method for treating enteropathogenic bacterial infections in poultry, comprising: (a) selecting a species of Lactobacillus reuteri, which is characterized by producing β-hydroxypropionaldehyde under anaerobic conditions in the presence of glycerol or glyceraldehyde; (b) administering an effective amount of cells of said species to said poultry; and (c) treating said poultry with an effective amount of gentamicin.
2. The method according to claim 1, wherein said administration comprises spraying said poultry with said cells in an amount sufficient to colonize the gastrointestinal tract of said poultry.
3. The method according to claim 1, wherein said administration comprises feeding said poultry with a feed containing Lactobacillus reuteri cells.
4. The method according to claim 3, wherein the feed contains about 1 O
5. 5 CFU of Lactobacillus reute ri gram food. The method according to claim 2, wherein said administration further comprises feeding said shorebirds with an aliquot containing cells from Lactobaci llu reute ri.
6. 6. The method according to claim 5, wherein the feed contains approximately 1 05 CFU of Lactobacillus reuteri per gram of feed.
7. 7. A food composition for poultry to treat bacterial enteropathogenic infections comprising Lactobacillus reuteri cells and an antibiotic. The composition according to claim 7, containing about 1 05 CDR of Lactobacillus reuteri per gram of said composition. 9. The composition according to claim 7, wherein the antibiotic is gentamicin. 1. The use of Lactobacillus reuteri cells to prepare a poultry feed composition, to treat bacterial enteropathogenic infections, using an antibiotic. eleven . The use according to claim 10, wherein the Lactobacillus reuteri cells are used in the composition in an amount of about 1 05 CFU per gram of said composition. The use according to claim 10, wherein said antibiotic is gentamicin.