MXPA05005401A - Live organism product - Google Patents

Abstract

A live organism product wherein the organisms are in a dormant state and are suspended in a liquid carrier which is substantially moisture-free. The carrier is comprised of oil. The carrier may also include an adsorbent. The product is stored and shipped in a plastic bag and is sprayed onto its target host or the like. The moisture and pH then activates the organisms.

Description

PRODUCT WITH LIVE ORGANISMS BACKGROUND D? THE INVENTION 1. FIELD OF THE INVENTION This invention relates to a product with latent organisms and more particularly to a product with living latent organisms, wherein living organisms, which are in a latent state, are suspended in a carrier or medium Liquid fluid that can be easily pumped and will remain in suspension during the life of the product. More particularly, the carrier, while in a liquid state, is free of moisture to prevent organisms from becoming activated until the product is applied to food, fodder or directly on animals or the like. Then in the present, food, fodder, animals or the like will be referred to as "the target host". 2. DESCRIPTION OF THE RELATED TECHNIQUE Formulations with live bacteria are often applied to forage products, foods, etc., to increase fermentation and / or digestion. The prior art products generally fall into two categories which are either a dry soluble powder form or a dry granular non soluble form. Dry granular products are applied directly to your target host through a dosing device such as a box Gandy, usual at a ratio of 4 to 16 ounces per ton of treated forage. The dry soluble products must first be hydrated with water, then applied to their target host within a period of 48 hours after hydration, with the application made through a liquid pumping system in the proportion of 37 grams to 1 gallon per ton of forage. Dry granular products of the prior art, even if packed in plastic buckets, sometimes come into contact with ambient air during manufacturing and processing or by simply opening the lid of the product with ambient air containing moisture or moisture . Moisture or moisture will activate latent bacteria in the contact that in turn causes the bacteria to live and inadvertently die before they can be applied to the forage. The value of the product will then be a total loss. The limestone carriers of the prior art products will carry very small amounts of moisture, and such moisture severely reduces the ability of the product to sustain an adequate shelf life. If the product of the prior art is a soluble powder product, it also needs to be hydrated before application. The hydration immediately activates all latent bacteria and the producer has to apply the complete mixture within a 48 hours or earlier, since bacteria will naturally deplete their food source and die, creating a total loss of product value. In addition, the prior art products in the current market must be either removed from their original packaging container or physically emptied in a dry applicator before application, or they must be contaminated with water, reducing their shelf life to less 48 hours, after contamination. Additionally, the dry granular products of the prior art have a tendency to separate during packing. This drives any of the fines to the bottom of the packaging container leaving the material either larger or lighter that works in its passage to the top. Since bacteria attach themselves to these particles, they can also migrate with these particles. Other dry soluble powder products can • settle to the bottom of the applicator tank during hydration. This will greatly depend on the dilution ratio of the product. As the proportions of application decrease to meet the current needs of the producer and bring less product to the field, manufacturers must in turn make their products more condensed to meet these needs. Such a thing limits the solubility of the product. , The dry granular products of the prior art they require that a significant percentage of the product be applied. to the material, which results in uniform coverage becoming difficult. Soluble dry powders mixed with water carriers have tried to obtain low inclusion rates for some time. However, such products result in droplets that are very large in their size and molecular weight resulting in there actually being few droplets that are supplied by treatment, an example being a ton of treated forage with only ten large droplets for coverage uniform is deficient. BRIEF DESCRIPTION OF THE INVENTION A product with live bacteria is disclosed in which the live bacteria are in a dormant state and are suspended in a carrier or clear fluid medium that can be easily pumped and will remain in suspension during the life of the product. More particularly, the carrier or medium for living bacteria is comprised of a liquid substantially free of moisture which is comprised of: (1) mineral oil and polymers; or (2) a processed oil obtained from either animal, vegetable or petroleum origin. In some cases, the carrier will also have a hydrophilic molecular sieve absorber (moisture scavenger) included therein. The product of this invention is preferably applies to the target host a micro-treatment ratio of 2 to 28 grams per ton of target host that is treated. In some cases, more or less percentages can be used. Live bacteria, when suspended in the moisture-free carrier, will remain dormant until they come into contact with the target host, at which time the moisture in the target host will cause the bacteria to be activated. The product of this invention is packaged in controlled packaging containers such as polyurethane bags that reduce the risk of moisture contamination of any kind. The product is sold and shipped directly to the producer, who will then take the collapsible product bag and hang it directly to an applicator who applies the product to the target host. The target host may be fodder, feed or the like. In some cases, the product can be sprayed directly on animals such as chickens who will ingest the product while they clean themselves. Although the product is ideally an improved live bacterial product, the product can be organisms. living latents freeze-dried that require environmental humidity, to initiate proliferation including, but not limited to, modified live bacterial and viral vaccines such as Parvo, Bovi'ne Rhinotracheitis, Leptospira, BVD, IBR and P13 or modified and unmodified pathogens. Therefore, a main object of the invention is to provide a product with improved living latent organisms that requires environmental humidity to begin proliferation. Yet another aspect of the invention is to provide a product with living latent organisms where living organisms that are dormant, are suspended in a carrier or clear liquid fluid medium that can be easily pumped and remain in suspension for the life of the product. Still another object of the invention is to provide a product with living latent organisms that is free of moisture to prevent it from becoming activated until the product is applied to the target host. Still another object of the invention is to provide a product with living latent organisms that can be applied to a target host at an extremely small proportion. Still another object of the invention is to provide a product with living latent organisms wherein the carrier or means is therefore comprised of a liquid substantially free of moisture comprised of: (1) mineral oil and polymers; or (2) a processed oil obtained from either animal, vegetable or petroleum origin.

Still another object of the invention is to provide a product with living latent organisms that will not be activated until it comes into contact with the moisture associated with the target host. Still another object of the invention is to provide a product with living latent organisms that can be applied to the forage or feed at a preferred micro-treatment rate. Furthermore, it is an object of the invention to provide a product with live latent organisms that is packaged in such a manner to limit its exposure to possible contamination from external sources, such as moisture charged air or flowing water of any kind. Still another object of the invention is to provide a product with living latent organisms such as modified and unmodified live bacterial and viral vaccines. These and other objects will be apparent to those skilled in the art. DETAILED DESCRIPTION OF THE INVENTION In the following, the product of this invention is described as a product with living latent bacteria. However, it must be understood that the product could be: (1) a living latent organism that requires environmental humidity to begin proliferation; (2) live or modified bacterial and viral vaccines such as Parvo, Bovine Rhinotracheitis, Leptospira, BVD, IBR and P13; or (3) modified and unmodified pathogens. In this invention, live viable harmless bacteria are mixed with a carrier or liquid medium substantially free of moisture. In general, the live bacteria used in the product of this invention will be any of the lactic acid producing bacteria that are permissible for use in animal-related products. The United States Food and Drug Administration (FDA) and The American Association of Feed Control Officials (AAFCO) have published a list of microorganism species that are "generally recognized as safe" (GRASS) for use in direct-feed microbial products . Table I lists the bacteria currently approved for use with animal-related products. Table I 36.14 - DFM Microorganisms under the state; GRASS "Aspergillus niger Aspergillus oryzae Lactobacillus curvatus Lactobacillus delbrue ii Bacillus coagulans Bacillus lentus Lactobacillus fermentum Lactobacillus helveticus Lactobacillus lactis Bacillus licheniformis Bacillus pumilus Lactobacillus plantarum Lactobacillus Bacillus subtilis Bacteroides amylophilus euterii Leuconostoc mesenteroides Bacteroides capillosus Pediococcus acidilacticii Bacteroides ruminocola Pediococcus cerevisiae Bacteroides suis Pediococcus pentosaceus Bifidobacterium adolescentis Propionibacterium freudenreichii Bifidobacterium animalis Propionibacterium shermanii Bifidobacterium bifidum Saccharomyces cerevisiae Bifidobacterium infantis Entercococcus cremoris Bifidobacterium longum Entercococcus diacetilactis Bifidobacterium thermophilum Entercococcus faecium Lactobacillus acidolphilus Entercococcus intermedius Lactobacillus brevis Lactobacillus buchneri Entercococcus lactis Lactobacillus bulgaricus Entercococcus thermophilius Lactobacillus casei Yeast (as defined elsewhere) Lactobacillus cellobiosus Preferably, the moisture free liquid carrier is comprised of a product marketed under the trademark "Synergel®" manufactured by Penreco of 138 Petrolia Street, Karns City, Pennsylvania 16041-9799. The product is generally referred to as a gelled mineral oil, reference number 1004-100, which has Brookfield viscosity of 1242 cPs. The carrier product is clear, odorless and insoluble in water. The carrier also includes polymers. Other suspending agents such as Cobisal (polymeric fiber) can also be used from time to time to help or reduce the possibility of bacteria settling therein. While the moisture-free oil described above is preferred, a processed oil obtained from either animal, vegetable or oil can be used. The product of this invention is designed to only activate the bacteria at the physical point of contact with the target host. At contact, the ambient moisture content of the target host will activate the latent bacteria found within the medium or liquid microbial carrier. The medium or liquid microbial carrier of this invention has been subjected to a special production process developed by Penreco. The process consists of first passing the mineral oil through a filtration system that specifically directs and absorbs any environmental humidity. Secondly, the mineral oil is heated and the polymers are added to increase the viscosity of the oil and the qualities of the suspension. Third, the oil is packaged in controlled packaging containers that reduce the risk of contamination by moisture of any kind. In the reception of the packaged carrier, the person who applies then preferably adds a product moisture scrubber such as a hydrophilic molecular sieve absorber as a guarantee program. The absorbent may comprise a natural or synthetic zeolite consisting of crystalline metal aluminosilicate, alkali metal aluminosilicate or sodium aluminosilicate. Finally, then any of the bacteria that are necessary for a particular purpose will be added. The final mixture is then packaged in moisture and ultraviolet retardant containers such as collapsible polyurethane bags very similar to I.V. The product is then sold and sent directly to the retailer and / or end user. The end user will then take the collapsible bag of liquid microbial mix and hang it directly to an applicator so that extremely small droplets are created that are sprayed onto the target hosts. The invention described herein provides the ability to inoculate other products with living latent bacteria, by means of a light spray application. While bacteria are protected with an oil / polymer mixture that allows host bacteria to survive longer in an unfavorable environment, coating the cell walls of the bacteria with the oil / polymer cover provides a physical barrier, chemistry against moisture. The above provides the ability to allow live bacteria to be introduced into a pellet feed without inadvertently activating the host oil / polymer / bacteria through contamination of environmental humidity from the food itself. The product of this invention allows it to be applied to its target host at a preferred micro-treatment ratio of 2 to 28 grams per one ton of forage or treated material (without additional water or necessary carriers). In some cases, higher or lower ratios may be employed. Such micro-treatment is substantially less than prior art products having treatment ratios ranging from 37.88 grams to 8.3453 pounds per one ton of treated fodder. The product of this invention and its packaging limits its exposure to possible contamination from external sources, such as moisture-laden air or flowing water of any kind. The product of this invention is designed to only activate at the physical point of contact with the target host. In contact with the target host, the change in pH and humidity activates latent bacteria. Thus, it can be seen that the invention accomplishes at least all of its stated objectives.

Claims (27)

1. CLAIMS 1. The method for forming stabilized mixtures of latent viable organisms, characterized in that it comprises: (a) preparing a mixture by intermixing a liquid carrier substantially free of moisture and the latent organism. The method according to claim 1, characterized in that the liquid carrier substantially free of moisture is selected from either mineral oil and polymers or a processed oil obtained from animal, vegetable or petroleum origin. 3. The method according to claim 1, characterized in that the mixture includes a hydrophilic molecular sieve adsorbent. 4. The method of compliance with the claim 3, characterized in that the adsorbent comprises a natural or synthetic zeolite. 5. The method of compliance with the claim 4, characterized in that the zeolite comprises crystalline metal aluminosilicate. 6. The method according to claim 4, characterized in that the zeolite comprises alkali metal aluminosilicate. The method according to claim 4, characterized in that the zeolite comprises aluminosilicate of sodium. 8. The method according to claim 1, characterized in that the mixture is sprayed on a target host with the humidity and pH that cause the activation of the organism. . The method according to claim 8, characterized in that the mixture is sprayed on the target host at a rate of 2 to 28 grams per ton of forage material. 10. The method of compliance with the claim 1, characterized in that the mixture is sprayed on a target host with the environmental humidity and pH that cause the activation of the organism. The method according to claim 5 8, characterized in that the mixture is sprayed on the target host at a rate of 2 to 28 grams per ton of feed material. 12. A stabilized mixture for application to food, feed or animals, or the like, characterized or comprising: a liquid carrier substantially free of moisture; and latent viable organisms suspended in the carrier. The mixture according to claim 12, characterized in that the liquid carrier substantially free of moisture is comprised of oil. 14. The mixture according to claim 12, characterized in that the mixture includes a hydrophilic molecular sieve adsorbent. 15. The mixture according to claim 14, characterized in that the adsorbent comprises a natural or synthetic zeolite. 16. The mixture according to claim 15, characterized in that the zeolite comprises crystalline metal aluminosilicate. 17. The mixture in accordance with the claim 15, characterized in that the zeolite comprises alkali metal aluminosilicate. 18. The mixture according to claim 15, characterized in that the zeolite comprises sodium aluminosilicate. 19. The mixture according to claim 12, characterized in that the mixture is sprayed on the target host with the humidity and pH that cause the activation of the organism. 20. The mixture in accordance with the claim 19, characterized in that the mixture is sprayed on the target host at a rate of 2 to 28 grams per ton of forage material. 21. The mixture according to claim 12, characterized in that the mixture is sprayed on a target host with the humidity and pH that cause the activation of the organism. 22. The mixture according to claim 21, characterized in that the mixture is sprayed on the target host at a rate of 2 to 28 grams per ton of target host. 23. The mixture according to claim 12, characterized in that the mixture is sprayed on the target host. 24. The method of compliance with the claim 1, characterized in that the mixture is sprayed on a target host. 25. The mixture according to claim 12, characterized in that the organism comprises a live bacterial vaccine. 26. The mixture according to claim 12, characterized in that the organism comprises a live viral vaccine. 27. The mixture according to claim 12, characterized in that the organism comprises a modified or unmodified pathogen.