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Application filed by Centro Inmunologia MolecularfiledCriticalCentro Inmunologia Molecular
Priority to MXPA02010471priorityCriticalpatent/MXPA02010471A/en
Publication of MXPA02010471ApublicationCriticalpatent/MXPA02010471A/en
The invention relates to a method of obtaining mammal cells, which are adapted to grow in a medium free of serum and proteins. The inventive method includes a process consisting of two adaptation steps comprising a critical protein concentration interval, in which the cells must acquire the ability to survive in a protein-free medium. There is a specific critical protein concentration interval for each cell line. The invention also relates to mammal cell lines, which grow in a stable manner in a medium free of serum and proteins over at least 40 generations. The invention further relates to clones, which express anti-CD6 T1hT and EGF hR3 antireceptor humanised antibodies and the anti-CD3 T3Q chimeric antibody as well as fragments of said antibodies.
MXPA020104712002-10-232002-10-23Method of obtaining cell lines in a protein-free medium and cell lines thus obtained.
MXPA02010471A
(en)
Process for preparing monoclonal antibodies; hybridoma cell lines generated by this process and recombinant chimeric virus-like particles with inserted foreign protein fragments as immunogenes to generate hybridomas by this process
Composition for stabilizing survival of transplanted hematopoietic stem cell, kit for obtaining the composition, method of stabilizing survivial of transplanted hematopoietic stem cell, human monoclonal antibody or human polyclonal antibody and method of producing the same, gene encoding human monoc