MXPA00000299A - Skin care composition - Google Patents

Abstract

Organic chick pea extracts are phytoestrogens, if present in an amount such as to provide an estrogenic activity equivalent to at least 1 nM of estradiol. Cosmetic compositions containing organic chick pea extracts are useful in improving the appearance of wrinkled, lined, dry, flaky, aged or photodamaged skin and improving skin thickness, elasticity, flexibility and plumpness.

Description

COMPOSITION FOR SKIN CARE FIELD OF THE INVENTION Cosmetic compositions containing an organic extract of chick-pea and methods for conditioning the skin by applying such compositions to the skin.

BACKGROUND OF THE INVENTION Human skin consists of two main layers, the thicker lower layer, the dermis, and the thinner upper layer, the epidermis. The dermis is the layer that provides resistance, elasticity and thickness to the skin. The main cell types of the dermis are the fibroblasts, which are responsible for the synthesis and secretion of all the dermal matrix components such as collagen, elastin and glycosides. . Collagen provides resistance, elasticity elastin and aminoglycosis glycosides moisture and skin thickness. With the undergoing, the thickness of the dermal layer is reduced and this is believed to be partially responsible for the formation of wrinkles in aged skin. The upper layer of human skin or epidermis, which promotes the resilience and barrier properties of the skin, is composed of many different types of cells including k e r t i no c t s, melanocytes and langerhans cells. The ke r a t i no ci t o s are the largest cell type of the epidermis (75-80% of the number of cells in the human epidermis). Richards, et al., Reported that estrogen stimulates the secretion of a protein, prolactin, by human dermal fibroblast cells and that prolactin then stimulates the proliferation of keto ats. Richards et al., Human Dermal Fibroblats Express Prolactin In Vitro., J. Invest. Dermatol., 106: 1250, 1996. Estrogens and synthetic compounds which act as estrogens are known to increase the thickness of the dermal layer and reduce the formation of wrinkles in aged skin. Changes in the skin such as dry skin, loss of elasticity of the skin and thickness that occur after menopause are attributed to the lack of estrogen production. Estrogen therapy prevents or delays many of these changes associated with aging skin. (Creidi et al., Effect of a conjugated estrogen cream on aging facial skin, Maturitas, 19, p 211, 1994). Some of the effects of estrogen on the skin include: increase in the thickness of the skin and disappearance of fine wrinkles, increase in the fungal proportion of the epidermis, reduction in the size and activity of the sebaceous gland, retarded growth rate of the hair, stimulation of the production of collagen and increase in the production of hyaluronic acid and synthesis of g 1 i co s ami nog 1 i of the fibroblasts (Pugliese, Menopausal skin, Skin Inc., March / April 1994: p 69 -77). In recent years, estrogens (that is, natural compounds, which have estrogen-like activity, which are found in plants) have been used incidentally for therapeutic purposes. Some of the uses described are as agents hipocoles te ro 1 émi cos yantiater ogéni co s, treatment of cardio-vascular diseases, especially in post-menopausal women, treatment for osteoporosis in the elderly and as an anticancer agent especially against cancer. breast, endometrial and cervical cancer in women (Knight et al., Phytoestrogens - a short review, Maturitas, 22: 167-75, 1995).

Consumer demand for products with "natural" bases has grown in recent years. Consumers perceive chemical synthesis as ambitious and unsafe. A chemically synthesized ingredient may contain chemicals. Natural products are perceived as pure and soft and superior to chemically synthesized products. However, providing a cosmetic benefit from a plant source is not trivial. In order to derive a real benefit from a "natural" source, not just a plant or part of a plant that contains a specific asset "has to be idetified., but also a minimum concentration and / or a specific extract of the plant has to be identified which truly provides a cosmetic benefit. More than 500 compounds present in plants have been described as having estrogenic activity. These compounds, collectively called phytoestrogens, are found in a large number of plants including cereals, legumes (including chickpeas) and grasses (Price et al., Naturally occurring estrogens in foods- a review., Food additives and contaminants, 2 , pp. 73-106, 1985). Their concentrations vary in the different parts of the plants, geographical position, year of growth, etc. The two main classes of plant compounds which possess phytogenic activity are the flavonoids and the coumestans. Some of the commonly described fibrogenic compounds are genistein, biocanin A, forommonone, daidzein and its glycoside derivatives (Knight et al., Phy t oe str ogen s -a short review., Maturitas, J. Climactreic and post-menopa use, 22, p.167-75, 1995). The chickpea or the Spanish pea (C icerarietun um), a lentil of the common diet, contains flavo no ides that include daidzein, fo rmoñone t ina, biocanina A, pratenseina, ferno ferreir in a, edicarpina, maackiaina, metilcoumestrol , medicagol, f ormonone t ina glucoside and biocanin A glucoside (Ingham et al., In. progress in the chemistry of organic natural products, vol 43: Ed-W. Herz et al., Sp r inge r- Ve r 1 ag, Wien, New York, 1983). It has been reported that chickpea flavonoids have effects that decrease lipids in the blood and liver of rats. Several studies nu trition to 1 € on the chickpea protein to be used as nutritional supplements 1 and ways to improve the protein quality of the chickpea Vasiliou, US Patent 4,761,285 describes the use of chickpeas as a supplement For example, in India, a cosmetic mask or skin treatment, made from chickpea powder mixed with water, is a common beauty treatment, WO 96/38162 describes a variety of dietetic or internal treatment or topical treatment. uses of lectins derived from chickpeas, including the restoration of the appearance of wrinkled skin The technique discussed above does not describe organic chickpea extracts for skin care or cosmetic use.The technique does not teach a topical application of organic extracts of chickpeas. chickpea, as the fit oe str oge no, and with a specified estrogenic activity.

BRIEF DESCRIPTION OF THE INVENTION The present invention includes a skin care composition comprising: (i) an organic extract of chickpeas in an amount that provides an estrogenic activity equivalent to at least 1 nM of estradiol, the estrogenic activity is determined by the test described in Example 1 of the description; (ii) a cosmetically acceptable vehicle. The present invention also includes a method for improving or preventing the condition of photodamaged, aged, scaly, dry, wrinkled, or lined skin and improving the thickness, elasticity, flexibility and thickness of the skin, which method includes applying the skin the inventive composition. The compositions of the invention are intended for topical application on the skin of mammals, which is already photodamaged, aged, wrinkled, scaly, dry, with lines, or the inventive compositions can be applied prophylactically to normal healthy skin to prevent or reduce deteriorating changes. The present invention also includes a cosmetic method for increasing fibroblasts and the proliferation of epidermal skin cells in human skin by applying the inventive composition to the skin.

DETAILED DESCRIPTION OF THE INVENTION Chickpeas Chickpeas are suitable for use in the inventive compositions in the form of an organic extract. The chickpea extract is prepared for use in the present invention from dried chickpeas. Dry chickpeas can be obtained from Arrowhead Mills, from health food stores or markets. The organic extracts of chickpea will be prepared by extracting the dried chickpeas "with a solvent stirring 1 part of dried chickpeas with 2 to 5 parts of solvent for 4 to 24 hours at room temperature.The suitable solvents are described below. they are classified by filtration and / or centrifugation, then dried by evaporation (optionally, under vacuum) to obtain the organic extract of chickpea.The suitable solvents for the preparation of the chickpea extract to be used here include, but are not limited to: ethanol, methanol, hexane, chloroform, dichloromethane, and ethyl acetate The preferred solvents are diclone or methanol, methanol, or ethanol to optimize the activity.The extract can be further concentrated, fractionated, re-extracted or purified, for example , by extraction of the organic solvent or by chromatography It has been found, as part of the present invention, that the organic extract of "chickpea" s must be used in a specific minimum amount to provide an estrogenic activity. The chickpea extract or powder is employed in an amount of 0.0001 to 50%, while providing the equivalent estrogenic activity, to at least 1 nM, generally in the range of 1 nM to 100 nM, of estradiol. The estrogenic activity of the chickpea extract is determined by comparing the activity of 1 nM of estradiol using __c and 1 u 1 to s ZR-75 in a side-by-side experiment, as described in Example 1 below. Preferably, the chickpea extract is employed in an amount such as to provide the estrogenic activity equivalent to at least 2 nM of estradiol.

Cosmetically Acceptable Vehicle _ __ The composition according to the invention also comprises a cosmetically acceptable vehicle to act as a diluent, dispersant or vehicle of the chickpea extract in the composition, to facilitate its distribution when the composition is applied to the skin. Other vehicles other than, or in addition to water may include liquid or solid emollients, solvents, humectants, thickeners and powders. A specifically preferred non-aqueous vehicle is a polydimethyl siloxane and a phenyl p or 1-dimethyloxy siloxane. The silicones of this invention can be those with viscosity ranging from about 10 to 10, 000,000 mm2 (centistokes) at 250 ° C. Especially desirable are low and high viscosity silicone blends. These silicones are available from General Electric Company under the trademarks Vicasil, SE and SF and from the Dow Corning Company under the 200 and 550 series. The amounts of silicone that can be used in the compositions of this invention range from 5% to 95%. %, preferably from 25% to 90% by weight of the composition. The cosmetically acceptable vehicle will usually form from 5% to 99.9%, preferably from 25% to 80% by weight of the composition and can, in the absence of other cosmetic auxiliaries, form the balance of the composition. Preferably, the vehicle is at least 80% by weight of water, by weight of the vehicle. Preferably, the water comprises at least 50% by weight of the inventive composition, more preferably, from 60 to 80% by weight, by weight of the composition.

Optional Cosmetic and Skin Beneficial Materials An oily or oily material may be present, along with a built-in emulsion, to provide either a water-in-oil emulsion or an oil-in-water emulsion, depending largely on the average balance hydrophilic-lipophilic (HLB) of the used urine. Inventive compositions for example include sunscreens. Sunscreens include those materials commonly used to block light from the sun. Illustrative compounds are the derivatives of PABA, cinnamate and silicilate. For example, octyl me t oxy cinnamate and 2-hydroxy-4-methoxy benzophenone (also known as oxybenzone) can be used. Octyl methoxamine and 2-hydroxy-4-methoxybenzophenone are commercially available under the trademarks Parsol MCX and B e n z op h e n on e-3, respectively. The exact amount of sunscreen used in the emulsions can vary depending on the degree of protection desired from the radiation of the sun's UV rays. Very often emollients are incorporated in the cosmetic compositions of the present invention. The levels of such emollients can vary from 0.5% to 50%, preferably between 5% and 30% by weight of the total composition. Emollients can be classified under such general chemical categories as esters, fatty acids and alcohols, polyols and hydrocarbons. The esters can be mono or diesters. Acceptable examples of the fatty diesters include dibutyl adipate, diethyl sebacate, diisopropyl dimerate, and dioctyl succinate. Acceptable branched chain fatty esters include 2-e t i 1-h e x i 1 myristate, isopropyl stearate and isostearyl palmitate. Acceptable tribasic acid esters include tri linoleate of t r i i s op r op i 1 o and trilauryl citrate. Acceptable straight chain fatty esters include lauryl palmitate, myristyl lactate and stearyl oleate. Preferred esters include coco-c ap r i l o / c ap r a t o (a mixture of co-octopus and coconut), propylene glycol myristyl ether acetate, diisopropyl adipate and ceyl octanoate. Suitable fatty acids and alcohols include those compounds having from 10 to 20 carbon atoms. Especially preferred are those compounds such as stearyl, palmitic, myristyl, and cetyl acids and alcohols. Among the polyols that can serve as emollients are the compounds at 1 qu or Ip or 1 ih i d r o i 1 o of straight and branched chain. For example, propylene glycol, sorbitol and glycerin are preferred. Polymeric polyols such as po 1 ipr op i 1 eng li co 1 and polyethylene glycol can also be useful. Butylene and propylene glycol are also especially preferred as penetration enhancers. The specimens that can serve as emollients are those that have hydrocarbon chains of 12 to 30 carbon atoms. Specific examples include mineral oil, petrolatum, squalene and isoparaffins. Another category of functional ingredients within the cosmetic compositions of the present invention are thickeners. A thickener will usually be present in amounts from 0.1 to 20% by weight, preferably from about 0.5% to 10% by weight of the composition. Exemplary thickeners are crosslinked polyacrylate materials available under the trademark Carbopol from B.F. Gooodrich Company. Gums such as xanthan, carrageenan, gelatin, karaya, pectin and locust bean gum can be used. Under certain circumstances the thickening function can be achieved by a material that also serves as a silicone or emollient. For example, silicone gums in excess of 10 centistokes and esters such as glycerol stearate have dual functionality. Powders can be incorporated in the cosmetic composition of the invention. These powders include calcium carbonate, talc, kaolin, starch, smectite mud, chemically modified magnesium aluminum silicate, modified montmorillonite clay, hydrous aluminum silicate, fumed silica, octenyl aluminum starch succinate, and mixtures thereof. Other minor auxiliary components can also be incorporated into the cosmetic compositions. These ingredients may include coloring agents, opacifiers and perfumes.

Amounts of these other minor auxiliary components may vary between 0.001% to 20% by weight of the composition.

Use of the Composition The composition according to the invention is intended primarily as a product for topical application to human skin, especially as an agent for conditioning, moisturizing and softening the skin, increasing its thickness, flexibility and elasticity and preventing or reducing the appearance of aged skin, wrinkled or with lines. During use, a small amount of composition, for example, from 1 to 100 ml, is applied to the exposed areas of the skin, of an appropriate applicator or container and, if necessary, then spread on and / or rubbed inside the skin using your hand or fingers or a suitable device.

Form and Product Packaging __ _ The topical skin care composition of the invention can be formulated as a lotion, cream or gel. The composition can be packaged in a suitable container to suit its viscosity and its intended use by the consumer. For example, a lotion or cream can be packaged in a bottle or in a roll on applicator or an aerosol device activated by a propellant or a container conditioned with a pump suitable for operation by means of the finger. When the composition is a cream, it can simply be stored in a non-deformable bottle to a container which can be tightened, such as a tube or a can with a lid. The composition can also be included in capsules, such as that described in US Pat. No. 5,063,507, incorporated herein by reference. The invention, therefore, also provides a closed container containing a cosmetically acceptable composition as defined herein. The following specific examples further illustrate the invention, but the invention is not limited thereto. All p-values in the Examples were calculated using a student's t-test.

EXAMPLE 1 This example illustrates that the organic extract of chickpeas at a concentration such as to provide a physical activity equivalent to at least 1 nM of estradiol is suitable for use in the present invention.

Preparation of chickpea extracts: Dry chickpeas were obtained from Ar r or whe ad Mills - (Hareford, TX) and ground to a powder in a dry mill. Chickpea powder of this mode obtained was extracted using dichloromethane (DCM) or methanol (1: 3 w / v) by mixing the dry powder with a solvent for 20 hours at room temperature. The extracts were sorted by filtration and centrifuged. The extracts were then dried by evaporation under vacuum to obtain the dry extract. The extraction of 63 g of dried chickpea powder with DCM dissolved in 1.47 g of dry material; extraction of 60 g of chickpea powder with methanol dissolved in 2.434 g of dry material. "These amounts at extraction of 2,231% by DCM and 4.06% by methanol." This dry extract was then redissolved in dimethyl sulfoxide (DMSO) at a concentration of 50 μl / ul to dose the cells.

The Methodology Used to Determine the Speed of DNA Synthesis in Cells: The incorporation of 3H-thymidine by cultured cells was used in an evaluation of cell proliferation (ZR75 or keratinocyte). Thymidine is one of four of the oxindoles, which are the monomeric units of DNA. Before the cell division of a somatic cell, the complete genome of the cell undergoing cell division is replicated. This involves large-scale DNA synthesis by the cell and allows both cells to receive identical copies of the genetic material. When 3H-thymidine is included in the culture medium of the cells that are synthesizing the DNA in the cell division preparation then the labeled thymidine is incorporated into the newly synthesized DNA. The extent of incorporation of 3 H-thymidine into the cell population is proportional to the rate of DNA synthesis by this population of cells and therefore an indication of its cell proliferation.

The following test was used to determine if the organic extracts of chickpea have phytoestr ogé activity and if this activity is equivalent to at least 1 nM of estradiol: The cell line ZR75 is a ductal breast carcinoma cell line, originally isolated from the malignant mammary epithelium of a Caucasian woman of sixty-three years of age (Engel et al., Human breast cancer carcinoma iTi continuous culture: A review., Cancer Res., 38: 4327-4339, 1978). This cell line contains receptors for estrogen, progesterone and other steroid hormones, but responds through an increase in proliferation only to estrogen. The cell line contains specific high-affinity estrogen receptors. Therefore, this cell line is used to test estrogen-like activity (Markiewicz et al., In vitro bioassays of non-stero ida 1 phytoestrogens, J. Steroid Biochem.Molec- Biol., 45: 399-405, 1993 ). 1. ZR75 cells (from American Type Culture Collection, Rockville, Maryland) were cultured in RPMI1640 medium (from Gibco Life Technologies) with 10% fetal bovine serum FBS 100 units of penicillin per ml and 100 units of streptomycin per ml. The medium does not contain phenol red (a weak imitator of estrogen). The cells were seeded in a flask at a density of one million by 75 cm2. For the experiment, the cells were seeded in 24-well plates at 100,000 cells per ml per well. 2. After the culture for 24 hours, the medium was removed, the cells were washed with PBS, and 1 ml of RPMI 1640 was added without serum (but with streptomycin and penicillin). The solutions of chickpea extract broth in dimethyl sulfoxide (DMSO) and estradiol in water were prepared. Various concentrations of organic extract of chickpea and estradiol, as indicated in Table 1, were then dosed directly into each well. After another 24 hours, one μCi of thymidine [methyl-3H] was added to each well. The medium was removed after 24 hours. The cells were washed once in PBS, the PBS was completely removed and the cells were left on ice to be incubated with 1 ml of 10% TCA cavity (trichloroacetic acid) for 30 minutes. Plates were washed 3 times with 5% TCA to remove all traces of thymidine which was not incorporated into the cells. 500 μl of 0.1M sodium hydroxide was added to each well and the plates were incubated at room temperature for at least 30 minutes. 250 μl of each sample were transferred to flask flasks and then 5 ml of bead fluid was added, the bottles were counted for 5 minutes to obtain the tritium, each in a graduation. The data from the triplicate cavities were calculated as the% thymidine incorporated into the DNA compared to that of the control cavities that received no chickpea or estradiol extract. Values were expressed as average of the cavities in triplicate ± the standard deviation. The results that were obtained are summarized in Table 1. TABLE 1 * indicates a statistically significant stimulation of DNA synthesis compared to control at the p < 0.05. As can be seen from the results in Table 1, 1 nM (0.274 ng / ml) or higher concentrations of estradiol increased the DNA synthesis of ZR75 cells. A similar activity of this particular chickpea extract was obtained at a concentration of 0.1 μg / ml or higher. The organic extract of chickpeas is suitable for use in the inventive compositions in an amount such as to provide estrogenic activity equivalent to at least 1 nM of estradiol. For this particular extract, the concentration of 0.1 μg of chickpeas per 1 ml of medium supplied the required activity.

EXAMPLE 2 Example 1 was repeated twice with various concentrations of chickpea extract as indicated in Table 2. The results obtained are summarized in Table 2.

TABLE 2 It can be observed from the results in Table 1 (representing Experiment 1) and Table 2 that in the 3 separate experiments performed on 3 separate days using 3 different passages of ZR75 cells, the organic extract of chickpeas significantly stimulated the proliferation of ZR75 cells.

EXAMPLE 3 This example illustrates that ke r a t inoc i t o s treated with an organic extract of chickpeas excreted a substance which stimulated the proliferation of fibroblasts.

Culture of keratinocyte cells: human kerat inocytes, isolated from the neonatal foreskin by means of trypsin treatment, were cultured in Dulbecco Modification Eagle (DME) Hams F12 (3: 1) fetal bovine serum medium / 5% in the presence of 3T3 mouse fibroblasts treated with mitomycin C to establish the colonies of ke rat divided inocula. Cells were cultured under the previous condition until their second passage and kept frozen for future use. The second frozen passage of ke r a t inoc i t o s was thawed and plated into the above-mentioned medium and cultured for five days. On day 5, when the cells were 70-80% confluent, they were tripinized and plated at 20, 000 - 30, 0 OU per cavity in 24-well plates in a ke growth medium. rat serum free inoculation (KGM-Clonetics, San Diego, CA), which contains 0.15 mM calcium. The cells were cultured at 80% confluence for 5 days before the experiment.

Fibroblast cell culture: Neonatal human foreskin fibroblasts obtained from Clonetics Corporat in (San Diego, CA), were cultured in DMEM containing 10% fetal bovine serum (both obtained from Life Technologies, Grand Island, NY) . The experiments were carried out on cells in their fifth to fifteenth passage. The cells were plated at 7,000-10,000 cells per cavity in 24-well plates and cultured up to 80% confluence for 5 days before the experiment.

Preparation of the conditioned medium with ke rat in ocitos: For the experiments that seek the interaction of ke ratinocitosy fibroblasts in response to the treatment with the chickpea extracts, the conditioned medium was prepared with ke ratinocytes as follows: 80% of -confluence of ke rat Inoculated as described above, it was treated with various concentrations of chickpea extracts (as indicated in Table 3) for 24 hours in RPMI medium without serum and without phenol red. The medium collected from the plates is referred to as "conditioned medium with keratinocytes".

Test Methodology: Keratinocytes and fibroblasts were separated separately with various concentrations of the chickpea extract in 1 ml RPMI medium without serum or phenol red. The fibroblasts were also dosed with 1 ml of medium conditioned with keratinocytes. After 24 hours, 3 H-thymidine was added at 1 μCi per well and the cells were incubated for 24 hours. The amount of H-thymidine associated with the cellular DNA was evaluated as described in step 2 of Example 1. The results that were obtained are summarized in Table 3.

TABLE 3 The results in Table 3 demonstrate that the chickpea extracts had no direct effect on the proliferation of fibroblasts or on the proliferation of keratinocytes. However, the chickpea extract induced the keratinocytes to secrete growth promoting substances for the fibroblasts since the keto-conditioned medium exposed to the chickpea extract significantly increased the proliferation of fibroblasts.

EXAMPLE 4 This example illustrates that organic extracts of chickpea include the proliferation of cells in an epidermal portion of the skin of the piglet. In this example, pure estradiol, f i t o e s t r o g i n g s, and chickpea extracts were tested for their effects on the proliferation of epidermal cells.

Piglet Skin Organ Culture: Piglet skin is morphologically and biochemically similar to human skin and is therefore commonly used to test the effects of materials for topical human use. Fresh shaved skin of piglets was obtained from the local trail, washed with Dove® soap and dermatomated at 200 μ thickness to obtain the upper epidermis and the dermal layer. Uniform striking biopsies of the dermatome piglet skin were taken using a 7mm puncher. The biopsies were washed in a medium containing high an- tibiotic / antimicrobial mixture (Kanamycin and penicillin and Gib- st streptomycin) and incubated for 3 days in a DMEM medium with high content of glucose. hi dr oco r 11 s ona, L-glutamine, antibiotic and antimycotic in the absence of serum in plaques tr vi sity, 3 biopsies per well. The medium was fed from the bottom of the transcavity so that the epidermal side of the biopsies was in contact with the air. On the third day, the medium was changed to a fresh medium, and the biopsies were exposed to the various concentrations of various active in 1 μl of DMSO, as indicated in Table 4. 3 days later, the medium was changed, took reading of the assets and the biopsies were labeled with 10 μCi of 3H thymidine per well. After washing the biopsies in PBS, the epidermis was divided from the dermis by incubating the biopsies for 24 hours in a 2 M solution of sodium bromide with agitation. The epidermis was detached from the dermis using a clamp and dissolved in 1 ml 0.5N NaOH with incubation overnight at 60 ° C. 200 μl of the dissolved epidermis was used for the count by scintillation to determine the amount of incorporation of 3 H-thymidine into the epidermis. The data were calculated as the average cpm for at least 4 replicates, and were also calculated as percentages of the control biopsies that did not receive active. The results that were obtained are summarized in Table 4.

TABLE 4 It can be seen from the results in Table 4, that in 2 separate experiments, estradiol at 10 nM increased the proliferation of piglet epidermal cells (in experiment 2, the increase was statistically significant). Daidzein also demonstrated this effect, daidzein is more effective than natriuretic hormone estradiol 1. Another isoflavone, formononetin, had no significant growth stimulation effect on the piglet's epidermis, both the methanolic and dichloromethane extracts of the chickpea also had a growth promoting effect, the dichloromethane extract being more effective than the methanolic extract. Chickpea dichloromethane extract was even more effective than pure isoflavones or estradiol. The results demonstrate the beneficial effects of organic chickpea extract on skin cells in a model system in which it closely resembles human skin. Examples 5-10 illustrate the skin care compositions according to the present invention. The compositions can be processed in a conventional manner. They are suitable for cosmetic use. In particular, the compositions are suitable for application to skin damaged by ultraviolet rays, aged, scaly, dry, rough, wrinkled or with lines to improve the appearance and feel thereof as well as the application to healthy skin for avoid or delay the deterioration of it.

Example 5 This example illustrates a high internal phase of water-in-oil emulsion incorporating the inventive composition.

Brij 92 is polyoxyethylene ether 2) or 1 and i 1 i or E j p lo 6 This example illustrates an oil-in-water cream incorporating the inventive composition.

* Brij 56 is a cetyl alcohol POE (10 Alfol 16RD is cetyl alcohol E j emp lo 7 This example illustrates an alcoholic lotion incorporating the composition according to the invention% p / p GARBANZO AND METANOL EXTRACT 5 E j emp lo 8 This example illustrates another alcoholic lotion containing the inventive composition.

E j emp lo 9 This example illustrates a sun care cream incorporating the composition of the invention: The invention illustrates a composition for non-aqueous skin care incorporating the inventive combination. a polymer of dime ti Is i 1 i cona having a molecular weight of at least 50,000 and a viscosity of at least 10,000 centistokes at 25 ° C, available from GEC 2 a cyclic pentamer of dime ti 1 if 1 oxa not available from Dow Corning Corp. 3 dime ti 1 if 1 oxa tetramer, available from Dow Corning Corp. It should be understood that the specific forms of the invention illustrated and described herein are intended to be rpresentative only. Changes, including, but not limited to those suggested in this specification, can be made in the illustrated modes without departing from the clear teachings of the description. Accordingly, reference should be made to the following additional indications to determine the full scope of the invention.

Claims (5)

1. Skin care composition, comprising: (i) an organic extract of chickpea in an amount such as to provide an estrogenic activity equivalent to at least 1 nM estradiol, the estrogenic activity is determined by the test described in Example 1 of the description; (ii) a cosmetically acceptable vehicle.

2. Cosmetic method for supplying a human tissue to the human skin, the method comprises applying to the skin the composition according to claim 1.

3. Cosmetic method to improve the appearance of photodamaged, aged, scaly, dry, wrinkled or lined skin and improve the thickness, elasticity, flexibility and thickness of the skin, the method comprises applying to the skin the composition according to claim 1.

4. Cosmetic method for improving the proliferation of fibroblasts in human skin, the method comprises applying to the skin the composition of claim 1.

5. Cosmetic method for improving the proliferation of skin epidermal cells, the method comprises applying to the skin the composition of claim 1.