MX9710410A - Ensayo quimioluminiscente de union especifica sin separacion. - Google Patents

Ensayo quimioluminiscente de union especifica sin separacion.

Info

Publication number
MX9710410A
MX9710410A MX9710410A MX9710410A MX9710410A MX 9710410 A MX9710410 A MX 9710410A MX 9710410 A MX9710410 A MX 9710410A MX 9710410 A MX9710410 A MX 9710410A MX 9710410 A MX9710410 A MX 9710410A
Authority
MX
Mexico
Prior art keywords
solid phase
tracer
specific binding
analyte
bound
Prior art date
Application number
MX9710410A
Other languages
English (en)
Other versions
MXPA97010410A (es
Inventor
Eve H Barlow
Eddie Carroll Iii
Joseph E Connolly
Michael J Lee
Richard A Martinelli
John T Unger
Original Assignee
Chiron Diagnostics Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Chiron Diagnostics Corp filed Critical Chiron Diagnostics Corp
Publication of MX9710410A publication Critical patent/MX9710410A/es
Publication of MXPA97010410A publication Critical patent/MXPA97010410A/es

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/536—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase
    • G01N33/542—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with steric inhibition or signal modification, e.g. fluorescent quenching
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/582—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with fluorescent label

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Food Science & Technology (AREA)
  • Biochemistry (AREA)
  • Cell Biology (AREA)
  • Biotechnology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Polysaccharides And Polysaccharide Derivatives (AREA)
  • Luminescent Compositions (AREA)

Abstract

El ensayo descrito en la presente es proveer una observacion de cuando los rastreadores marcados con éster de acridinio se unen a su conjugado de union correspondiente inmovilizado en una fase solida de oxido de metal, la emision de luz quimioluminiscente medible del rastreador marcado unido a la fase solida es extinguida segun comparado con el rastreador de fraccion libre que no está libre a la fase solida, de acuerdo con la invencion, se provee un ensayo de union especifica sin separacion para detectar o cuantificar la presencia de un analito, en donde la mezcla de reaccion completa es vaporizada (incluyendo el rastreador sin reaccionar) y la señal modulada (debido al efecto de extincion) está asociada con una referencia, determinando así la cantidad o presencia del analito en la muestara. Las desventajas inherentes de los análisis heterogéneos que emplean separaciones pueden ser evitadas utilizando este método sin separcion.
MXPA/A/1997/010410A 1995-06-29 1997-12-18 Ensayo quimioluminiscente de union especifica sinseparacion MXPA97010410A (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US08496549 1995-06-29
US08/496,549 US5783453A (en) 1995-06-29 1995-06-29 Non-separation specific binding chemiluminescent assay

Publications (2)

Publication Number Publication Date
MX9710410A true MX9710410A (es) 1998-07-31
MXPA97010410A MXPA97010410A (es) 1998-11-09

Family

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Also Published As

Publication number Publication date
US5783453A (en) 1998-07-21
BR9609390A (pt) 1999-05-18
EP0835451A1 (en) 1998-04-15
CA2224825A1 (en) 1997-01-16
AU700057B2 (en) 1998-12-17
EP0835451B1 (en) 1999-03-03
DE69601637D1 (de) 1999-04-08
WO1997001763A1 (en) 1997-01-16
JPH11508682A (ja) 1999-07-27
PL324312A1 (en) 1998-05-25
AU6013396A (en) 1997-01-30
DE69601637T2 (de) 1999-09-16
KR19990028561A (ko) 1999-04-15
ATE177208T1 (de) 1999-03-15

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