MX9605941A - Method for introducing foreign matter into higher eukaryotic cells. - Google Patents

Method for introducing foreign matter into higher eukaryotic cells.

Info

Publication number
MX9605941A
MX9605941A MX9605941A MX9605941A MX9605941A MX 9605941 A MX9605941 A MX 9605941A MX 9605941 A MX9605941 A MX 9605941A MX 9605941 A MX9605941 A MX 9605941A MX 9605941 A MX9605941 A MX 9605941A
Authority
MX
Mexico
Prior art keywords
foreign matter
eukaryotic cells
higher eukaryotic
introducing foreign
dna
Prior art date
Application number
MX9605941A
Other languages
Spanish (es)
Inventor
Matthew Cotten
Adam Baker
Susanna Chiocca
Original Assignee
Boehringer Ingelheim Int
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from DE4418825A external-priority patent/DE4418825A1/en
Priority claimed from DE19944442587 external-priority patent/DE4442587A1/en
Application filed by Boehringer Ingelheim Int filed Critical Boehringer Ingelheim Int
Publication of MX9605941A publication Critical patent/MX9605941A/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
    • C07K14/4701—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals not used
    • C07K14/4702—Regulators; Modulating activity
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
    • C07K14/4701—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals not used
    • C07K14/4747—Apoptosis related proteins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/52—Cytokines; Lymphokines; Interferons
    • C07K14/54—Interleukins [IL]
    • C07K14/5412—IL-6
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/52—Cytokines; Lymphokines; Interferons
    • C07K14/54—Interleukins [IL]
    • C07K14/5421—IL-8
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
    • C12N2710/00011—Details
    • C12N2710/10011—Adenoviridae
    • C12N2710/10211—Aviadenovirus, e.g. fowl adenovirus A
    • C12N2710/10222—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
    • C12N2710/00011—Details
    • C12N2710/10011—Adenoviridae
    • C12N2710/10311—Mastadenovirus, e.g. human or simian adenoviruses
    • C12N2710/10322—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Engineering & Computer Science (AREA)
  • Medicinal Chemistry (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Toxicology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Virology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Pharmacology & Pharmacy (AREA)
  • General Chemical & Material Sciences (AREA)
  • Veterinary Medicine (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Public Health (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)

Abstract

The toxicity problems arising when foreign matter is introduced into higher eukaryotic cells, especially with transfection with DNA, are obviated by expression in the cell of gene products that block the apoptosis induced by the transfection process and/or by treating the cells with anti-inflammatory substances. Preferred anti-apoptosis genes are Bcl-2, E1B 19K or an anti-apoptotic gene of the CELO avian adenovirus; used as preferred anti-inflammatory substance in the adenovirus VA1, which is introduced into the cell in the form of VA1 DNA. These measures help to achieve a long-lasting gene expression.
MX9605941A 1994-05-30 1995-05-26 Method for introducing foreign matter into higher eukaryotic cells. MX9605941A (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
DE4418825A DE4418825A1 (en) 1994-05-30 1994-05-30 Transfection of eukaryotic cells
DE19944442587 DE4442587A1 (en) 1994-11-30 1994-11-30 Transfection of eukaryotic cells
PCT/EP1995/001989 WO1995033062A2 (en) 1994-05-30 1995-05-26 Method for introducing foreign matter into higher eukaryotic cells

Publications (1)

Publication Number Publication Date
MX9605941A true MX9605941A (en) 1997-12-31

Family

ID=25937004

Family Applications (1)

Application Number Title Priority Date Filing Date
MX9605941A MX9605941A (en) 1994-05-30 1995-05-26 Method for introducing foreign matter into higher eukaryotic cells.

Country Status (8)

Country Link
US (2) US6284880B1 (en)
EP (1) EP0767840A2 (en)
JP (1) JPH10500578A (en)
AU (1) AU709148B2 (en)
CA (1) CA2190963A1 (en)
CO (1) CO4410254A1 (en)
MX (1) MX9605941A (en)
WO (1) WO1995033062A2 (en)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE19615803A1 (en) 1996-04-20 1997-10-23 Boehringer Ingelheim Int CELO virus
CA2304131A1 (en) * 1997-09-19 1999-04-01 James M. Wilson Method for gene transfer using bcl2 and compositions useful therein
CA2320513A1 (en) * 1998-02-11 1999-08-19 Joseph T. Bruder Vectors, cells and methods for the production of deleterious viral eukaryotic gene transfer vectors
GB9908195D0 (en) 1999-04-09 1999-06-02 Microbiological Res Authority Treatment of intracellular infection
US20050013870A1 (en) * 2003-07-17 2005-01-20 Toby Freyman Decellularized extracellular matrix of conditioned body tissues and uses thereof
US7033673B2 (en) * 2003-07-25 2006-04-25 Analytical Services & Materials, Inc. Erosion-resistant silicone coatings for protection of fluid-handling parts
US20070178137A1 (en) * 2006-02-01 2007-08-02 Toby Freyman Local control of inflammation
JP2010536345A (en) * 2007-08-14 2010-12-02 グラクソスミスクライン・リミテッド・ライアビリティ・カンパニー Novel methods and cell lines

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5354844A (en) 1989-03-16 1994-10-11 Boehringer Ingelheim International Gmbh Protein-polycation conjugates
US5521291A (en) 1991-09-30 1996-05-28 Boehringer Ingelheim International, Gmbh Conjugates for introducing nucleic acid into higher eucaryotic cells
NZ244306A (en) 1991-09-30 1995-07-26 Boehringer Ingelheim Int Composition for introducing nucleic acid complexes into eucaryotic cells, complex containing nucleic acid and endosomolytic agent, peptide with endosomolytic domain and nucleic acid binding domain and preparation

Also Published As

Publication number Publication date
WO1995033062A3 (en) 1996-07-18
US6284880B1 (en) 2001-09-04
AU709148B2 (en) 1999-08-19
EP0767840A2 (en) 1997-04-16
CA2190963A1 (en) 1995-12-07
CO4410254A1 (en) 1997-01-09
JPH10500578A (en) 1998-01-20
AU2616095A (en) 1995-12-21
WO1995033062A2 (en) 1995-12-07
US20020049313A1 (en) 2002-04-25

Similar Documents

Publication Publication Date Title
Luzzati Regulation of λ exonuclease synthesis: Role of the N gene product and λ repressor
ATE282434T1 (en) A COMPOSITION CONTAINING A DETERGENT AND AN ADENOVIRAL VECTOR ENCODING A TUMOR SUPPRESSOR GENE
GEP20012559B (en) Complementary Adenoviral Vector Systems and Cells Lines
JPS57500830A (en)
ZA961484B (en) Dna molecules preparation and use in gene therapy
IL148152A0 (en) Sequence-specific dna recombination in eukaryotic cells
AU5957986A (en) Vector for the expression of gamma-interferon in mammal cells, method for its implementation, product obtained and pharmaceutical composition containing the gamma-interferon
ATE505206T1 (en) USE OF THE MELANOMA DIFFERENTIATION ASSOCIATED GENE (MDA-7) TO CONVERT A CANCER PHENOTYPE
NO964445L (en) DNA sequence, vector, host cell and method for transfecting hematopoietic cells with exogenous DNA
Wilson et al. Modulation of plasminogen activator synthesis in chick embryo fibroblasts by cyclic nucleotides and phorobol myristate acetate
AUPN477695A0 (en) Gene therapy
MX9605941A (en) Method for introducing foreign matter into higher eukaryotic cells.
Morozkin et al. Extracellular nucleic acids in cultures of long‐term cultivated eukaryotic cells
Ajiro et al. Species-specific suppression of histone H1 and H2B production in human/mouse hybrids.
Gallily et al. Tumor necrosis factor as a mediator of Mycoplasma orale-induced tumor cell lysis by macrophages
DE69738822D1 (en) NEW BAC1-W MAMMAL, WHICH BELONGS TO THE BC1-2 FAMILY OF APOPTOSIS-CONTROLLING GENES
FI961537A0 (en) Butyrobetaine / crotonobetaine L-carnitine metabolism genes and their use in microbiological preparation of L-carnitine
Shafei-Benaissa et al. The naevoid basal-cell carcinoma syndrome (Gorlin syndrome) is a chromosomal instability syndrome
Ganguly et al. A cell-free assay using cytoplasmic cell extracts to study rejoining of radiation-induced DNA double-strand breaks in human cell nuclei
IL131436A0 (en) Compositions for treatment of disorders involving programmed cell death
Chida et al. Efficient and stable gene transfer following microinjection into nuclei of synchronized animal cells progressing from G1/S boundary to early S phase
Akins et al. Factors regulating the encystment enhancing activity (EEA) of Acanthamoeba castellanii
Regan Alkaline phosphatase in synchronized human cells
Cotten et al. Chicken embryo lethal orphan(CELO) virus GAM-1
Osato et al. In vitro inhibition of DNA, RNA, and protein syntheses by Shigella dysenteriae type 1 enterotoxin