MX353614B - Measuring instrument of diffusion coefficients by the fluorescence recovery technique. - Google Patents
Measuring instrument of diffusion coefficients by the fluorescence recovery technique.Info
- Publication number
- MX353614B MX353614B MX2014014236A MX2014014236A MX353614B MX 353614 B MX353614 B MX 353614B MX 2014014236 A MX2014014236 A MX 2014014236A MX 2014014236 A MX2014014236 A MX 2014014236A MX 353614 B MX353614 B MX 353614B
- Authority
- MX
- Mexico
- Prior art keywords
- fluorescence
- diffusion
- recovery
- measuring instrument
- instrument
- Prior art date
Links
Landscapes
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
Abstract
The Present disclosure is related to a measuring instrument of diffusion coefficient of molecules marked with fluorescence in liquid samples. The instrument uses the FRAP technique and consists of a laser beam that passes through the window of an acoustic optical modulator controlled electronically to attenuate the intensity of the laser beam. As a result, it is possible to obtain, at will, either a very intense beam for bleaching or erasing the fluorescence (high power) or a low intensity excitation beam (low power) which allows the residual fluorescence to be monitored. With the aid of other optical elements, the light beams are directed towards the sample to excite or destroy the fluorescence. Subsequently, the instrument measures the recovery of fluorescence due to diffusion of the fluorescent molecules in the surrounding area by means of a photon detector, sending the signal to a high speed card in a computer. The fluorescence recovery time allows obtaining the diffusion coeffici ent of the fluorescent molecule. By means of graphic programming, it is possible to control the sequence to send signals to the devices and measurements can be recorded. Finally, the recovery is plotted and the corresponding equations for obtaining diffusion coefficients are applied.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| MX2014014236A MX353614B (en) | 2014-11-07 | 2014-11-07 | Measuring instrument of diffusion coefficients by the fluorescence recovery technique. |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| MX2014014236A MX353614B (en) | 2014-11-07 | 2014-11-07 | Measuring instrument of diffusion coefficients by the fluorescence recovery technique. |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| MX2014014236A MX2014014236A (en) | 2016-05-09 |
| MX353614B true MX353614B (en) | 2017-12-06 |
Family
ID=56797816
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| MX2014014236A MX353614B (en) | 2014-11-07 | 2014-11-07 | Measuring instrument of diffusion coefficients by the fluorescence recovery technique. |
Country Status (1)
| Country | Link |
|---|---|
| MX (1) | MX353614B (en) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN117084618B (en) * | 2023-10-18 | 2024-04-30 | 深圳迈瑞生物医疗电子股份有限公司 | Method and device for controlling endoscope light source and endoscope image pickup system |
-
2014
- 2014-11-07 MX MX2014014236A patent/MX353614B/en active IP Right Grant
Also Published As
| Publication number | Publication date |
|---|---|
| MX2014014236A (en) | 2016-05-09 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| FG | Grant or registration |