MX2025008564A - Enzyme variants and uses thereof - Google Patents

Enzyme variants and uses thereof

Info

Publication number
MX2025008564A
MX2025008564A MX2025008564A MX2025008564A MX2025008564A MX 2025008564 A MX2025008564 A MX 2025008564A MX 2025008564 A MX2025008564 A MX 2025008564A MX 2025008564 A MX2025008564 A MX 2025008564A MX 2025008564 A MX2025008564 A MX 2025008564A
Authority
MX
Mexico
Prior art keywords
amino acid
polypeptides
acid sequence
hydrolyzing
seq
Prior art date
Application number
MX2025008564A
Other languages
Spanish (es)
Inventor
Matthew Spence
Vanessa Vongsouthi
Christopher Jonathan Matthews
Dana Suzanne Matthews
Jennifer Nhu Tuyet Nu Ton
Colin John Jackson
Original Assignee
Samsara Eco Pty Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from AU2023900157A external-priority patent/AU2023900157A0/en
Application filed by Samsara Eco Pty Ltd filed Critical Samsara Eco Pty Ltd
Publication of MX2025008564A publication Critical patent/MX2025008564A/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08J—WORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H
    • C08J11/00—Recovery or working-up of waste materials
    • C08J11/04—Recovery or working-up of waste materials of polymers
    • C08J11/10—Recovery or working-up of waste materials of polymers by chemically breaking down the molecular chains of polymers or breaking of crosslinks, e.g. devulcanisation
    • C08J11/105—Recovery or working-up of waste materials of polymers by chemically breaking down the molecular chains of polymers or breaking of crosslinks, e.g. devulcanisation by treatment with enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/78—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5)
    • C12N9/80—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5) acting on amide bonds in linear amides (3.5.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C12P13/001—Amines; Imines
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/40—Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
    • C12P7/44—Polycarboxylic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y305/00—Hydrolases acting on carbon-nitrogen bonds, other than peptide bonds (3.5)
    • C12Y305/01—Hydrolases acting on carbon-nitrogen bonds, other than peptide bonds (3.5) in linear amides (3.5.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y305/00—Hydrolases acting on carbon-nitrogen bonds, other than peptide bonds (3.5)
    • C12Y305/01—Hydrolases acting on carbon-nitrogen bonds, other than peptide bonds (3.5) in linear amides (3.5.1)
    • C12Y305/01046—6-Aminohexanoate-dimer hydrolase (3.5.1.46)
    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08J—WORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H
    • C08J2377/00—Characterised by the use of polyamides obtained by reactions forming a carboxylic amide link in the main chain; Derivatives of such polymers
    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08J—WORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H
    • C08J2377/00—Characterised by the use of polyamides obtained by reactions forming a carboxylic amide link in the main chain; Derivatives of such polymers
    • C08J2377/02—Polyamides derived from omega-amino carboxylic acids or from lactams thereof
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/20—Flavobacterium
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
    • Y02W30/00—Technologies for solid waste management
    • Y02W30/50—Reuse, recycling or recovery technologies
    • Y02W30/62—Plastics recycling; Rubber recycling

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biomedical Technology (AREA)
  • Polymers & Plastics (AREA)
  • Sustainable Development (AREA)
  • General Chemical & Material Sciences (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Polyamides (AREA)

Abstract

La presente descripción se relaciona generalmente a polipéptidos capaces de hidrolizar un enlace de amida en una poliamida, y más específicamente a polipéptidos que comprenden una secuencia de aminoácidos de los residuos de aminoácido 2-398 de SEQ ID NO: 2 o una secuencia de aminoácidos que tiene por lo menos 70% de identidad de secuencia a la misma; o polipéptidos que comprenden una secuencia de aminoácidos de los residuos de aminoácido 2-394 de SEQ ID NO: 88 o una secuencia de aminoácidos que tiene por lo menos 75% de identidad de secuencia a la misma. La presente descripción también se extiende a métodos para utilizar los polipéptidos. En otra modalidad, los polipéptidos descritos son capaces de hidrolizar el oligómero de nailon 6,6 para producir ácido adípico y hexametilendiamina.This description relates generally to polypeptides capable of hydrolyzing an amide bond in a polyamide, and more specifically to polypeptides comprising an amino acid sequence from amino acid residues 2-398 of SEQ ID NO: 2 or an amino acid sequence having at least 70% sequence identity to it; or polypeptides comprising an amino acid sequence from amino acid residues 2-394 of SEQ ID NO: 88 or an amino acid sequence having at least 75% sequence identity to it. This description also extends to methods for using the polypeptides. In another embodiment, the described polypeptides are capable of hydrolyzing the nylon 6,6 oligomer to produce adipic acid and hexamethylenediamine.

MX2025008564A 2023-01-23 2025-07-23 Enzyme variants and uses thereof MX2025008564A (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
AU2023900157A AU2023900157A0 (en) 2023-01-23 Enzyme variants and uses thereof
AU2023901918A AU2023901918A0 (en) 2023-06-16 Enzyme variants and uses thereof
PCT/AU2024/050035 WO2024156025A1 (en) 2023-01-23 2024-01-23 Enzyme variants and uses thereof

Publications (1)

Publication Number Publication Date
MX2025008564A true MX2025008564A (en) 2025-11-03

Family

ID=91969784

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2025008564A MX2025008564A (en) 2023-01-23 2025-07-23 Enzyme variants and uses thereof

Country Status (8)

Country Link
EP (1) EP4655397A1 (en)
JP (1) JP2026503586A (en)
KR (1) KR20250137166A (en)
CN (1) CN120641562A (en)
AU (1) AU2024211077A1 (en)
IL (1) IL322300A (en)
MX (1) MX2025008564A (en)
WO (1) WO2024156025A1 (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB202418775D0 (en) 2024-12-19 2025-02-05 Epoch Biodesign Ltd Processes and enzymes for use therein

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2736927B1 (en) * 1995-07-18 1997-10-17 Rhone Poulenc Fibres & Polymer ENZYMES HAVING AMIDASE ACTIVITY, GENETIC TOOLS AND HOST MICROORGANISMS FOR OBTAINING SAME AND HYDROLYSIS PROCESS USING THE SAME
CN101423478B (en) * 2007-11-02 2012-05-23 宁波大学 A method for producing adipic acid and hexamethylenediamine by depolymerization of nylon-66
EP4474375A4 (en) * 2022-02-02 2025-12-31 Asahi Chemical Ind METHOD FOR THE PREPARATION OF A DICARBONIC ACID COMPOUND AND/OR DIAMINE COMPOUND USING POLYAMIDE AS A STARTING MATERIAL

Also Published As

Publication number Publication date
AU2024211077A1 (en) 2025-07-31
WO2024156025A1 (en) 2024-08-02
JP2026503586A (en) 2026-01-29
EP4655397A1 (en) 2025-12-03
KR20250137166A (en) 2025-09-17
IL322300A (en) 2025-09-01
CN120641562A (en) 2025-09-12

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