MX2023006572A - Metodo para el tamizaje genetico de preimplantacion de embriones. - Google Patents

Metodo para el tamizaje genetico de preimplantacion de embriones.

Info

Publication number
MX2023006572A
MX2023006572A MX2023006572A MX2023006572A MX2023006572A MX 2023006572 A MX2023006572 A MX 2023006572A MX 2023006572 A MX2023006572 A MX 2023006572A MX 2023006572 A MX2023006572 A MX 2023006572A MX 2023006572 A MX2023006572 A MX 2023006572A
Authority
MX
Mexico
Prior art keywords
day
cfdna
genetic screening
medium
oocytes
Prior art date
Application number
MX2023006572A
Other languages
English (en)
Inventor
Clifford L Librach
Svetlana Madjunkova
Valeriy Kuznyetsov
Original Assignee
Reprobiogen Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Reprobiogen Inc filed Critical Reprobiogen Inc
Publication of MX2023006572A publication Critical patent/MX2023006572A/es

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1093—General methods of preparing gene libraries, not provided for in other subgroups
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0603—Embryonic cells ; Embryoid bodies
    • C12N5/0604—Whole embryos; Culture medium therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/156—Polymorphic or mutational markers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/16—Primer sets for multiplex assays

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Gynecology & Obstetrics (AREA)
  • Reproductive Health (AREA)
  • Pathology (AREA)
  • Plant Pathology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Cell Biology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Se describe un método no invasivo para la detección genética antes de la implantación del embrión. Los ovocitos fertilizados se cultivan el día 1, seguido de la eliminación de las células de corona/cumulus residuales, lo que reduce la contaminación materna; aislamiento de ovocitos y cultivo individual desde los días 1-4 con suplemento de proteína sérica; llevar a cabo la ruptura de la zona con láser el día 4 permitiendo que el ADN libre de células embrionarias (cfADN) entre en el medio de cultivo; lavar los ovocitos con medio fresco para producir un medio que contiene cfADN del día 4; transferir al medio nuevo bajo aceite y cultivar hasta el día 5, 6 o 7 para formar un blastocisto expandido que se transfiere el día 5/6/7 a una gota de medio; exponer el blastocisto expandido a un pulso de láser para extruir el líquido de blastocoel que contiene cfADN embrionario, obteniendo el día 5/6/7 cfADN; y la modalidad de exámenes genéticos de cfADN utilizando la amplificación del genoma completo (WGA). El método se puede usar para la detección genética de embriones en busca de aneuploidía mediante la prueba de WGA para el número de copias de cromosomas completos.
MX2023006572A 2020-12-04 2021-04-28 Metodo para el tamizaje genetico de preimplantacion de embriones. MX2023006572A (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US202063121463P 2020-12-04 2020-12-04
PCT/CA2021/050586 WO2022115937A1 (en) 2020-12-04 2021-04-28 Method for preimplantation genetic screening of embryos

Publications (1)

Publication Number Publication Date
MX2023006572A true MX2023006572A (es) 2023-07-20

Family

ID=81852667

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2023006572A MX2023006572A (es) 2020-12-04 2021-04-28 Metodo para el tamizaje genetico de preimplantacion de embriones.

Country Status (5)

Country Link
US (1) US20240002942A1 (es)
AU (1) AU2021393374A1 (es)
CA (1) CA3200368A1 (es)
MX (1) MX2023006572A (es)
WO (1) WO2022115937A1 (es)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
PL442092A1 (pl) * 2022-08-25 2024-02-26 Gyncentrum Spółka Z Ograniczoną Odpowiedzialnością Sposób nieinwazyjnego, preimplantacyjnego badania genetycznego zarodków
CN116515965A (zh) * 2023-03-02 2023-08-01 序康医疗科技（苏州）有限公司 一种筛选具有优良发育潜能胚胎的无创检测方法

Also Published As

Publication number Publication date
US20240002942A1 (en) 2024-01-04
AU2021393374A1 (en) 2023-07-06
CA3200368A1 (en) 2022-06-09
WO2022115937A1 (en) 2022-06-09

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