MX2021002439A - Crioconservación de sustratos sembrados con celulas y metodos relacionados. - Google Patents

Crioconservación de sustratos sembrados con celulas y metodos relacionados.

Info

Publication number
MX2021002439A
MX2021002439A MX2021002439A MX2021002439A MX2021002439A MX 2021002439 A MX2021002439 A MX 2021002439A MX 2021002439 A MX2021002439 A MX 2021002439A MX 2021002439 A MX2021002439 A MX 2021002439A MX 2021002439 A MX2021002439 A MX 2021002439A
Authority
MX
Mexico
Prior art keywords
cell
cryopreservation
related methods
features
seeded substrates
Prior art date
Application number
MX2021002439A
Other languages
English (en)
Inventor
Mark S Humayun
Cassidy Arnold
Danhong Zhu
Debbie Mitra
Britney O Pennington
Jeffrey K Bailey
Mohamed A Faynus
Lincoln V Johnson
Dennis O Clegg
David R Hinton
Original Assignee
Univ Southern California
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Southern California filed Critical Univ Southern California
Publication of MX2021002439A publication Critical patent/MX2021002439A/es

Links

Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • A61K35/30—Nerves; Brain; Eyes; Corneal cells; Cerebrospinal fluid; Neuronal stem cells; Neuronal precursor cells; Glial cells; Oligodendrocytes; Schwann cells; Astroglia; Astrocytes; Choroid plexus; Spinal cord tissue
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N1/00—Preservation of bodies of humans or animals, or parts thereof
    • A01N1/02—Preservation of living parts
    • A01N1/0205—Chemical aspects
    • A01N1/021—Preservation or perfusion media, liquids, solids or gases used in the preservation of cells, tissue, organs or bodily fluids
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N1/00—Preservation of bodies of humans or animals, or parts thereof
    • A01N1/02—Preservation of living parts
    • A01N1/0236—Mechanical aspects
    • A01N1/0263—Non-refrigerated containers specially adapted for transporting or storing living parts whilst preserving, e.g. cool boxes, blood bags or "straws" for cryopreservation
    • A01N1/0268—Carriers for immersion in cryogenic fluid, both for slow-freezing and vitrification, e.g. open or closed "straws" for embryos, oocytes or semen
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N1/00—Preservation of bodies of humans or animals, or parts thereof
    • A01N1/02—Preservation of living parts
    • A01N1/0278—Physical preservation processes
    • A01N1/0284—Temperature processes, i.e. using a designated change in temperature over time
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K48/00—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08G—MACROMOLECULAR COMPOUNDS OBTAINED OTHERWISE THAN BY REACTIONS ONLY INVOLVING UNSATURATED CARBON-TO-CARBON BONDS
    • C08G61/00—Macromolecular compounds obtained by reactions forming a carbon-to-carbon link in the main chain of the macromolecule
    • C08G61/02—Macromolecular compounds containing only carbon atoms in the main chain of the macromolecule, e.g. polyxylylenes
    • C08G61/025—Polyxylylenes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0618—Cells of the nervous system
    • C12N5/062—Sensory transducers, e.g. photoreceptors; Sensory neurons, e.g. for hearing, taste, smell, pH, touch, temperature, pain
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0618—Cells of the nervous system
    • C12N5/0621—Eye cells, e.g. cornea, iris pigmented cells
    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08G—MACROMOLECULAR COMPOUNDS OBTAINED OTHERWISE THAN BY REACTIONS ONLY INVOLVING UNSATURATED CARBON-TO-CARBON BONDS
    • C08G2261/00—Macromolecular compounds obtained by reactions forming a carbon-to-carbon link in the main chain of the macromolecule
    • C08G2261/30—Monomer units or repeat units incorporating structural elements in the main chain
    • C08G2261/34—Monomer units or repeat units incorporating structural elements in the main chain incorporating partially-aromatic structural elements in the main chain
    • C08G2261/342—Monomer units or repeat units incorporating structural elements in the main chain incorporating partially-aromatic structural elements in the main chain containing only carbon atoms
    • C08G2261/3424—Monomer units or repeat units incorporating structural elements in the main chain incorporating partially-aromatic structural elements in the main chain containing only carbon atoms non-conjugated, e.g. paracyclophanes or xylenes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/105—Insulin-like growth factors [IGF]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/113—Acidic fibroblast growth factor (aFGF, FGF-1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/115—Basic fibroblast growth factor (bFGF, FGF-2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/16—Activin; Inhibin; Mullerian inhibiting substance
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2523/00—Culture process characterised by temperature
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/30—Synthetic polymers

Landscapes

  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Cell Biology (AREA)
  • Neurology (AREA)
  • Neurosurgery (AREA)
  • Environmental Sciences (AREA)
  • Dentistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Ophthalmology & Optometry (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Developmental Biology & Embryology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Epidemiology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Immunology (AREA)
  • Virology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Acoustics & Sound (AREA)
  • Mechanical Engineering (AREA)
  • Hematology (AREA)
  • Molecular Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Polymers & Plastics (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Materials For Medical Uses (AREA)

Abstract

En la presente se describen métodos y composiciones para la identificación de características celulares que mejoran la viabilidad y características de sustrato en lo que se refiere a la supervivencia posterior a la crioconservación de células sembradas de sustrato. Las modalidades de la presente invención implican además la identificación de características celulares para fabricar un sobrenadante que es útil para el cultivo celular y el tratamiento de diversas enfermedades.
MX2021002439A 2018-09-07 2019-08-30 Crioconservación de sustratos sembrados con celulas y metodos relacionados. MX2021002439A (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201862728646P 2018-09-07 2018-09-07
PCT/US2019/049230 WO2020051097A1 (en) 2018-09-07 2019-08-30 Cryopreservation of cell-seeded substrates and related methods

Publications (1)

Publication Number Publication Date
MX2021002439A true MX2021002439A (es) 2021-05-27

Family

ID=69719237

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2021002439A MX2021002439A (es) 2018-09-07 2019-08-30 Crioconservación de sustratos sembrados con celulas y metodos relacionados.

Country Status (9)

Country Link
US (2) US11825839B2 (es)
EP (1) EP3847245A1 (es)
JP (2) JP7430885B2 (es)
KR (1) KR20210075986A (es)
CN (1) CN112689670A (es)
CA (1) CA3110745A1 (es)
MX (1) MX2021002439A (es)
SG (1) SG11202102140WA (es)
WO (1) WO2020051097A1 (es)

Family Cites Families (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4163828A (en) 1978-01-20 1979-08-07 Union Carbide Corporation Parylene stabilization
JP2003047464A (ja) 2001-08-02 2003-02-18 Institute Of Tsukuba Liaison Co Ltd 動物細胞の保存方法
US20030054331A1 (en) 2001-09-14 2003-03-20 Stemsource, Inc. Preservation of non embryonic cells from non hematopoietic tissues
US20050106554A1 (en) 2003-11-19 2005-05-19 Palecek Sean P. Cryopreservation of pluripotent stem cells
WO2005069766A2 (en) 2003-11-21 2005-08-04 The Regents Of The University Of California Self-contained cell culture apparatus and method of use
US20060063141A1 (en) * 2004-09-17 2006-03-23 Mcgann Locksley E Method of cryopreserving cells
JP5763288B2 (ja) 2005-11-17 2015-08-12 日本全薬工業株式会社 細胞保存用水溶液
JP2007306856A (ja) 2006-05-18 2007-11-29 Japan Health Science Foundation 細胞凍結保存方法
US7923823B2 (en) 2007-01-23 2011-04-12 Infineon Technologies Ag Semiconductor device with parylene coating
US20090123992A1 (en) 2007-11-12 2009-05-14 Milton Chin Shape-Shifting Vitrification Device
WO2009067601A1 (en) 2007-11-20 2009-05-28 Pioneer Surgical Orthobiologics, Inc. Cryopreservation of cells using cross-linked bioactive hydrogel matrix particles
US8808687B2 (en) 2010-07-12 2014-08-19 Mark Humayun Biocompatible substrate for facilitating interconnections between stem cells and target tissues and methods for implanting same
US20140045264A1 (en) * 2011-04-29 2014-02-13 University Of Southern California Method of cryopreservation of stem cell-derived retinal pigment epithelial cells on polymeric substrate
US8877489B2 (en) 2011-12-05 2014-11-04 California Institute Of Technology Ultrathin parylene-C semipermeable membranes for biomedical applications
WO2012149484A2 (en) * 2011-04-29 2012-11-01 University Of Southern California Method of cryopreservation of stem cell-derived retinal pigment epithelial cells on polymeric substrate
CA2792081C (en) 2011-10-11 2020-10-27 Bond University Ltd Layered compositions comprising 3d nanofibre webbing for tissue repair
US9850463B2 (en) 2012-02-01 2017-12-26 The Regents Of The University Of California Methods of culturing retinal pigmented epithelium cells, including xeno-free production, RPE enrichment, and cryopreservation
EP3323884A1 (en) 2013-02-01 2018-05-23 The United States Of America as Represented by the Secretary, Department of Health an Human Service Method for generating retinal pigment epithelium (rpe) cells from induced pluripotent stem cells (ipscs)
CA2997763A1 (en) 2015-09-08 2017-03-16 Cellular Dynamics International, Inc. Macs-based purification of stem cell-derived retinal pigment epithelium

Also Published As

Publication number Publication date
WO2020051097A1 (en) 2020-03-12
JP2021536264A (ja) 2021-12-27
CN112689670A (zh) 2021-04-20
CA3110745A1 (en) 2020-03-12
JP7430885B2 (ja) 2024-02-14
JP2024056712A (ja) 2024-04-23
SG11202102140WA (en) 2021-04-29
US20240049701A1 (en) 2024-02-15
US11825839B2 (en) 2023-11-28
EP3847245A1 (en) 2021-07-14
US20200077641A1 (en) 2020-03-12
KR20210075986A (ko) 2021-06-23

Similar Documents

Publication Publication Date Title
PH12019501557A1 (en) Bacillus isolates and uses thereof
BR112016029581A2 (pt) bactéria geneticamente modificada e métodos de modificação genética de bactéria
WO2018097540A3 (ko) 무혈청면역세포배양용 배지첨가키트, 상기 키트를 이용한 면역세포배양방법, 상기 키트 또는 배양방법에 의해 얻어진 무혈청면역세포배양액 및 상기 배양액을 포함하는 화장료조성물
MX2021012751A (es) Agentes de control biologicos modificados y sus usos.
NZ704125A (en) Bacillus megaterium bioactive compositions and metabolites
MX2015013050A (es) Bacterias que promueven el crecimiento de plantas y metodos de uso.
MX358751B (es) Microbios que promueven el crecimiento vegetal y uso de éstos.
PH12016502148A1 (en) Methods of microalgae cultivation for increased resource production
MX2019008002A (es) Composiciones microbianas y metodos.
IN2014DN08862A (es)
PH12018502061A1 (en) Differentiation of pluripotent stem cells to intestinal midgut endoderm cells
WO2018217064A3 (ko) 형질전환된 t세포를 이용한 자연살해세포의 배양방법
MX2020011254A (es) Celulas t car anti-bcma para agotamiento de celulas de plasma.
MX2021009554A (es) Produccion de virus en cultivos celulares.
MY192824A (en) Compound targeting il-23a and tnf-alpha and uses thereof
WO2016081032A3 (en) Mesenchymal stem cells derived from induced pluripotent stem cells
MX2013005140A (es) Medios de cultivo de celulas granulados en seco.
WO2016176620A8 (en) Extracellular matrix compositions for the treatment of cancer or immunological diseases
PH12016500222A1 (en) Compounds and methods for improving plant performance
SG11201806177QA (en) Transplant material for treatment of heart disease
EA201990128A1 (ru) Культуры клеточных линий из растений, принадлежащих к роду harpagophytum
MX2022004373A (es) Celulas madre modificadas y metodos de uso de las mismas.
MX2021007160A (es) Una semilla de planta artificial y usos de esta.
MX2023010875A (es) Tiburón arcoíris transgénico.
WO2019103528A3 (ko) 무혈청 배지 조성물