MX2020003362A - Modified strains for the production of recombinant silk. - Google Patents

Modified strains for the production of recombinant silk.

Info

Publication number
MX2020003362A
MX2020003362A MX2020003362A MX2020003362A MX2020003362A MX 2020003362 A MX2020003362 A MX 2020003362A MX 2020003362 A MX2020003362 A MX 2020003362A MX 2020003362 A MX2020003362 A MX 2020003362A MX 2020003362 A MX2020003362 A MX 2020003362A
Authority
MX
Mexico
Prior art keywords
modified strains
production
recombinant silk
strains
modified
Prior art date
Application number
MX2020003362A
Other languages
Spanish (es)
Inventor
Matthew Scott Gamboa
Joshua Tyler Kittleson
Original Assignee
Bolt Threads Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Bolt Threads Inc filed Critical Bolt Threads Inc
Publication of MX2020003362A publication Critical patent/MX2020003362A/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14—Fungi; Culture media therefor
    • C12N1/16—Yeasts; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/43504—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates
    • C07K14/43513—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates from arachnidae
    • C07K14/43518—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates from arachnidae from spiders
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/58—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from fungi
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/23—Aspartic endopeptidases (3.4.23)
    • C12Y304/23041—Yapsin 1 (3.4.23.41)

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Mycology (AREA)
  • Biophysics (AREA)
  • Insects & Arthropods (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Toxicology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Botany (AREA)
  • Virology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Peptides Or Proteins (AREA)

Abstract

Disclosed herein are modified strains for reducing degradation of recombinantly expessed products secreted from a host organism and methods of using the modified strains.
MX2020003362A 2017-10-03 2017-10-03 Modified strains for the production of recombinant silk. MX2020003362A (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PCT/US2017/054997 WO2019070246A1 (en) 2017-10-03 2017-10-03 Modified strains for the production of recombinant silk

Publications (1)

Publication Number Publication Date
MX2020003362A true MX2020003362A (en) 2020-07-29

Family

ID=65994932

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2020003362A MX2020003362A (en) 2017-10-03 2017-10-03 Modified strains for the production of recombinant silk.

Country Status (7)

Country Link
EP (1) EP3692054A4 (en)
JP (1) JP7246102B2 (en)
KR (1) KR102558303B1 (en)
CN (1) CN111315763A (en)
AU (2) AU2017434920B2 (en)
MX (1) MX2020003362A (en)
WO (1) WO2019070246A1 (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
MX2020012243A (en) 2018-05-17 2021-01-29 Bolt Threads Inc Sec modified strains for improved secretion of recombinant proteins.
WO2023215221A2 (en) * 2022-05-02 2023-11-09 North Carolina State University Engineered microorganisms with enhanced protein expression and secretion

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7972809B2 (en) * 2002-04-26 2011-07-05 National Institute Of Advanced Industrial Science & Technology Methylotrophic yeast producing mammalian type sugar chain
US7262287B2 (en) * 2002-06-29 2007-08-28 Korea Research Institute Of Bioscience And Biotechnology Hansenula polymorpha yapsin deficient mutant strain and process for the preparation of recombinant proteins using the same
AU2003263163A1 (en) * 2002-09-20 2004-04-08 Cym1P A/S Methods for increasing the production of a recombinant polypeptide from a host cell
ES2539378T3 (en) * 2009-02-26 2015-06-30 Glaxosmithkline Llc Host cells and use procedures
US8440456B2 (en) * 2009-05-22 2013-05-14 Vib, Vzw Nucleic acids of Pichia pastoris and use thereof for recombinant production of proteins
BR112014015933A2 (en) * 2011-12-30 2019-09-24 Oxyrane Uk Ltd methods and materials to reduce recombinant protein degradation
CN102676563B (en) * 2012-05-03 2013-09-18 浙江大学 Method for preparing human serum albumin-human parathyroid hormone
CA2924343A1 (en) * 2013-09-17 2015-03-26 Bolt Threads, Inc. Methods and compositions for synthesizing improved silk fibers
CN106701813A (en) * 2016-12-29 2017-05-24 陕西慧康生物科技有限责任公司 Expression vector as well as construction method and application thereof

Also Published As

Publication number Publication date
EP3692054A4 (en) 2021-06-09
CN111315763A (en) 2020-06-19
JP7246102B2 (en) 2023-03-27
KR102558303B1 (en) 2023-07-21
JP2021503275A (en) 2021-02-12
EP3692054A1 (en) 2020-08-12
AU2022202534A1 (en) 2022-05-12
KR20200058482A (en) 2020-05-27
AU2017434920A1 (en) 2020-05-07
AU2017434920B2 (en) 2022-02-10
WO2019070246A1 (en) 2019-04-11

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