MX2015004373A - Nucleic acid molecules for increased protein production. - Google Patents

Nucleic acid molecules for increased protein production.

Info

Publication number
MX2015004373A
MX2015004373A MX2015004373A MX2015004373A MX2015004373A MX 2015004373 A MX2015004373 A MX 2015004373A MX 2015004373 A MX2015004373 A MX 2015004373A MX 2015004373 A MX2015004373 A MX 2015004373A MX 2015004373 A MX2015004373 A MX 2015004373A
Authority
MX
Mexico
Prior art keywords
mutated
increased protein
nucleic acid
acid molecules
signal peptide
Prior art date
Application number
MX2015004373A
Other languages
Spanish (es)
Inventor
Knapp Andreas
Voget Sonja
Daniel Rolf
Jaeger Karl-Erich
Original Assignee
Basf Se
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Basf Se filed Critical Basf Se
Priority to MX2015004373A priority Critical patent/MX2015004373A/en
Publication of MX2015004373A publication Critical patent/MX2015004373A/en

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  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

The present invention relates to the improved production of proteins, preferably enzymes such as lipases. In particular, the invention relates to a mutated signal peptide and nucleotide sequence encoding said signal peptide that results in an increased protein secretion. Further, the invention relates to a mutated promoter that results in an increased protein expression. It was surprisingly found that the combination of the mutated signal peptide and the mutated promoter act synergistically to result in an about 100-fold increased protein production. Nucleic acid molecules, expression vectors and host cells comprising the mutated signal peptide, mutated promoter, or the combination thereof are also encompassed by the invention. Finally, the invention relates to methods and uses of such nucleic acid molecules, expression vectors and host cells for protein preparation.
MX2015004373A 2015-04-07 2015-04-07 Nucleic acid molecules for increased protein production. MX2015004373A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
MX2015004373A MX2015004373A (en) 2015-04-07 2015-04-07 Nucleic acid molecules for increased protein production.

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
MX2015004373A MX2015004373A (en) 2015-04-07 2015-04-07 Nucleic acid molecules for increased protein production.

Publications (1)

Publication Number Publication Date
MX2015004373A true MX2015004373A (en) 2016-10-06

Family

ID=58161142

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2015004373A MX2015004373A (en) 2015-04-07 2015-04-07 Nucleic acid molecules for increased protein production.

Country Status (1)

Country Link
MX (1) MX2015004373A (en)

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