MX2015004373A - Nucleic acid molecules for increased protein production. - Google Patents
Nucleic acid molecules for increased protein production.Info
- Publication number
- MX2015004373A MX2015004373A MX2015004373A MX2015004373A MX2015004373A MX 2015004373 A MX2015004373 A MX 2015004373A MX 2015004373 A MX2015004373 A MX 2015004373A MX 2015004373 A MX2015004373 A MX 2015004373A MX 2015004373 A MX2015004373 A MX 2015004373A
- Authority
- MX
- Mexico
- Prior art keywords
- mutated
- increased protein
- nucleic acid
- acid molecules
- signal peptide
- Prior art date
Links
Landscapes
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Enzymes And Modification Thereof (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The present invention relates to the improved production of proteins, preferably enzymes such as lipases. In particular, the invention relates to a mutated signal peptide and nucleotide sequence encoding said signal peptide that results in an increased protein secretion. Further, the invention relates to a mutated promoter that results in an increased protein expression. It was surprisingly found that the combination of the mutated signal peptide and the mutated promoter act synergistically to result in an about 100-fold increased protein production. Nucleic acid molecules, expression vectors and host cells comprising the mutated signal peptide, mutated promoter, or the combination thereof are also encompassed by the invention. Finally, the invention relates to methods and uses of such nucleic acid molecules, expression vectors and host cells for protein preparation.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
MX2015004373A MX2015004373A (en) | 2015-04-07 | 2015-04-07 | Nucleic acid molecules for increased protein production. |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
MX2015004373A MX2015004373A (en) | 2015-04-07 | 2015-04-07 | Nucleic acid molecules for increased protein production. |
Publications (1)
Publication Number | Publication Date |
---|---|
MX2015004373A true MX2015004373A (en) | 2016-10-06 |
Family
ID=58161142
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
MX2015004373A MX2015004373A (en) | 2015-04-07 | 2015-04-07 | Nucleic acid molecules for increased protein production. |
Country Status (1)
Country | Link |
---|---|
MX (1) | MX2015004373A (en) |
-
2015
- 2015-04-07 MX MX2015004373A patent/MX2015004373A/en unknown
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