MX2013001546A - Composition of ethanol extracts of larrea tridentata and fluorensia cernua with antimicrobial activity. - Google Patents
Composition of ethanol extracts of larrea tridentata and fluorensia cernua with antimicrobial activity.Info
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- MX2013001546A MX2013001546A MX2013001546A MX2013001546A MX2013001546A MX 2013001546 A MX2013001546 A MX 2013001546A MX 2013001546 A MX2013001546 A MX 2013001546A MX 2013001546 A MX2013001546 A MX 2013001546A MX 2013001546 A MX2013001546 A MX 2013001546A
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Abstract
The invention refers to a process for obtaining and using a composition of ethanol extracts mainly comprising tannins resulting from the leaves of Larrea tridentata and Fluorensia cernua. The composition presents hydrolyzed tannins and condensed tannins, which have antimicrobial activity against Fusarium sp., Rhizoctonia sp., Sclerotinia sp. and Seclerotium sp. such as soil phytopathogenic fungi, against Alternaria sp., Colletotrichum sp., Cercospora sp. and Pestalotia sp. such as foliar phytopathogenic fungi and against Clavibacter michiganensis sp. and Ralstoniasp such as phytopathogenic bacteria in agricultural crops, where the composition presents a 100% inhibiting action against the growth of said microorganisms. The ethanol extracts are finally mixed based on a formula for determining the optimum concentration for inhibiting the phytopathogenic microorganisms.
Description
COMPOSITION OF ETHANOLIC EXTRACTS OF GOVERNOR LARREA TRIDENTATA AND HOJASÉN FLOURENSIA CERNUA WITH ACTIVITY
ANTIMICROBIAL
DESCRIPTION
OBJECT OF THE INVENTION
The present creation refers to the obtaining and mixing of ethanolic extracts of governor Larrea tridentata and hojaén Flourensia cemita with the purpose of increasing the antimicrobial potential, as well as its use against phytopathogenic microorganisms of agricultural crops.
BACKGROUND
United States patent US 792, 462 consists of a natural composition, for the cleaning of tubes and boilers based on aqueous extracts of Larrea tridentata and Covillea tridentata, whose proportions are 1 gallon (3.78 L) of water, 12 oz (340.19 g) of Larrea tridentata and 4 oz (113.39 g) of Covillea tridentata, so the composition is more focused on cleaning pipes at home or industrial level, where they use the mixture of plant extracts of similar species . On the other hand, in the present work, the innovation consists of the use of a mixture of ethanolic extracts of two species of plants of the Mexican Semidesert, such as the governor Larrea tridentata and hojaén Flourensia cernua, whose proportions are 150 g of plant dust
dehydrated and 600 mL of water / ethanol and this composition focuses on the agricultural sector as antimicrobial.
US Pat. No. 290,269 speaks of a medical composition for the treatment of diseases in humans such as gonorrhea, dysentery, diarrhea and rheumatism, from aqueous extracts of Limoncillo Pectis species, Huayacan Porliera angustifolia and governor Covillea tridentata DC and Larrea tridentata DC, in a proportion of 56.69-113.39 g of plant / 3.78 L of water. On the other hand, the present invention consists of an antimicrobial composition for the agricultural sector based on ethanolic extracts of Governor Larrea tridentata and Hojasén Flourensia cernua, to inhibit and / or control phytopathogenic microorganisms of agricultural crops.
US Pat. No. 5,663,209 deals with a process of separation, purification and characterization of compounds derived from nordihydroguaracetic acid (NDGA), contained in the plant of governor Larrea tridentata, with the purpose of suppressing the chain of activation and replication of HIV TAT, so that the potential use of Larrea tridentata is more focused on medicine and thus on human health. The present work consists of a process to obtain the ethanolic extracts of Governor Larrea tridentata and Hojasén Flourensia cernua without going through a process of separation and characterization of compounds, so the potential of the mixture of these two ethanol extracts positively affects the increase of its antimicrobial activity against phytopathogenic microorganisms of agricultural crops, since all the biologically active compounds present in the composition, such as polyphenols, act by presenting synergism or antagonism with other molecules present and thus acquire and / or increase their antimicrobial capacity.
US patent 5, 927, 005 consists of the use of leaves and stems of governor Larrea tridentata for the phytoremediation of soils contaminated with heavy metals such as lead, copper, nickel, cadmium, mercury, arsenic, selenium, strontium and zinc; In this work, leaves and stems of the governor plant Larrea tridentata are used as the only plant, without obtaining extracts, contributing to the ecology of the environment. On the other hand, the present novelty is more focused on agriculture through the use of a composition based on two-plant ethanolic extracts such as the governor Larrea tridentata and hojaén Flourensia cernua with the purpose of controlling and / or inhibiting phytopathogenic microorganisms of crops agricultural.
The European patent WO2000028959 Al, mentions the use of cosmetic or dermopharmaceutical compositions based on aqueous extracts of Larrea tridentata and Larrea divaricada without nordihydroguayaretic acid, to delay the aging of the skin, stop hair loss and the treatment of mucous membranes. In contrast, in the present work, the novelty consists of an antimicrobial composition based on ethanolic extracts from the governor Larrea tridentata and hojaén Flourensia cernua to inhibit and / or control the presence of soil phytopathogenic fungi, foliar phytopathogenic fungi and phytopathogenic bacteria of crops. with the purpose of avoiding certain diseases in crops caused by this type of microorganisms.
The patent of the United States of North America W09917609 Al, consists of a process of obtaining a non-toxic extract of Larrea tridentata, by means of organic solvents such as propylene glycol, glycerin and ethanol to then saturate the extract with ascorbic acid and in this way reduce the toxic compounds such as quinones derived from nordihydroguaracetic acid; as well as its use for the treatment of viral diseases
caused by viruses of the Herpesviridae family or viruses that require the SP1 class of viral proteins to initiate repetitions, as an anti-inflammatory in diseases mediated by leukotrienes, to stabilize the toxic compounds of the nordihydroguaracetic acid and as a food additive. In the present study, water and ethanol are used as solvents during the process of obtaining and mixing the ethanolic extracts of Governor Larrea tridentata and Hojasén Flourensia cernua, in order to increase the presence of polyphenolic compounds in the mixture and / or composition. antimicrobial in the agricultural sector, against soil phytopathogenic fungi, phytopathogenic foliar fungi and phytopathogenic bacteria of agricultural crops.
The Mexican patent MX 107279 deals with a process for obtaining fodder for domestic animals made from the plant of governor Larrea tridentata by subjecting it to a solution of water-caustic soda in a proportion of 12 to 18%, it is worth mentioning that only the governor Larrea tridentata is used as the only plant. In the present invention, the treatment of the plant material of two species of Mexican semi-desert plants, such as the governor Larrea tridentata and hojaén Flourensia cernua, is mentioned, where they are first subjected to an outdoor drying and then to an oven drying, for later pulverize the leaves and stems of the plant species; as well as the method of obtaining and mixing the extracts using water and ethanol as solvents, with the purpose of obtaining ethanolic extracts from governor Larrea tridentata and hojaén Flourensia cernua for later application as an antimicrobial composition of agricultural crops.
The Mexican patent MX28070 is a composition based on mule grass, governor, mountain arnica, chamomile, olive oil, water and petroleum jelly for the
treatment of the scalp. On the other hand, the present study consists of an antimicrobial composition directed to the agricultural sector based on ethanolic extracts of governor Larrea tridentata and hojasén Flourensia cernua.
Mexico is considered one of the five countries with the greatest biodiversity in the world, which is why it is classified as a mega diverse country, since it has at least 10% of the terrestrial diversity of the planet, however, the alteration of habitats by the use indiscriminate and excessive use of synthetic pesticides is negatively affecting numerous ecosystems. This is why in our country about 206 species of plants have been studied against 26 species of phytopathogenic fungi, observing that some extracts of them have an antifungal effect during the germination of spores, mycelial development and sporulation of the fungi; likewise, this biocidal effect has been demonstrated in greenhouse and field tests. In several research works it has been possible to formulate organic products from the plants in presentations of aqueous, metabolic, ethanolic and hexane extracts; as well as in the form of powders, essential oils and secondary antifungal metabolites. In general terms, between 32 and 21% of the plants studied interact with fungi and bacteria, and the responses of the pathogens vary from biological stimulation to total inhibition [R. Montes-Belmont. Mexican Journal of Phytopathology, 18: 125-131. 2000].
The Mexican patent application NL / a / 2006/000057"Natural compounds with antimicrobial activity to prevent and control infectious diseases in humans and food" consists in the use of water-soluble resinous methanolic and ethanolic extracts of Governor Larrea tridentata, extracts and essential oils of leaves of Lippia graveolens, Lippia berlandieri, agave de lechuguilla, hojaén Flourensia cernua and others
plants known to have antimicrobial effects. And its function as bactericidal or bacteriostatic organic, antiseptic and harmless environmental disinfectant to prevent or cure diseases caused by bacteria that attack humans, animals, raw and processed foods as well as any other product susceptible to being infected. It is worth mentioning that the present novelty mentions the use of a natural and organic composition based on ethanolic extracts of Governor Larrea tridentata and hojaén Flourensia cernua, whose applications are more focused on the agricultural sector as an antimicrobial against soil phytopathogenic fungi of the genus Fusarium sp. ., Rhizoctonia sp., Sclerotinia sp. and Sclerotium sp., foliar phytopathogenic fungi of the genus Alternaria sp., Colletotrichum sp., Cercospora sp. and Pestalotia sp., as well as phytopathogenic bacteria of the genus Clavibacter michiganensis sp. and Ralstonia sp. of agricultural crops of chili, potato, lettuce, cabbage, garlic, onion, tomato, papaya, soy and mango.
BRIEF DESCRIPTION OF THE FIGURES
Figure 1 shows the graph of the effect of the composition of ethanolic extracts of Governor Larrea tridentata and Hojasén Flourensia cernua on the percentage of growth inhibition of phytopathogenic fungi of agricultural crops, with their respective control.
Figure 2 shows the graph of the effect of the composition of the ethanolic extracts of Governor Larrea tridentata and Hojasén Flourensia cernua on the percentage of inhibition of the growth of foliar phytopathogenic fungi of agricultural crops, with their respective control.
Figure 3 shows the graph of the effect of the composition of ethanolic extracts of governor Larrea tridentata and hojaén Flourensia cernua on the percentage of inhibition of the growth of phytopathogenic bacteria of agricultural crops, with their respective control. Figure 4 shows the standard curve of hydrolysable tannins equivalent to gallic acid.
Figure 5 shows the graph of the content of hydrolysable tannins equivalent to gallic acid, condensed tannins equivalent to catechin and total tannins in mg / L of the ethanolic extract of governor Larrea tridentata.
Figure 6 shows the graph of the standard curve of condensed tannins equivalent to catechin.
Figure 7 shows the graph of the content of hydrolysable tannins equivalent to gallic acid, condensed tannins equivalent to catechin and total tannins in mg / L of the ethanolic extract of leaves Flourensia cernua.
DETAILED DESCRIPTION OF THE INVENTION
The process and obtaining a composition containing a mixture of ethanolic extracts from governor Larrea tridentata and hojaén Flourensia cernua with antimicrobial activity, which comprises the steps of:
Stage a) Dehydration of leaves and stems: leaves and stems of governor Larrea tridentata and hojaén Flourensia cernua outdoors are first dehydrated, exposing the plant material to sunlight for 45-60 hours, preferably 48 hours, then the plant material placed in a stove to dehydrate it for the second time at temperatures of 50-60 ° C, preferably at 60 ° C within a time range of 45-60 hours, preferably 48 hours, in order to remove the water contained in the material and concentrating the polyphenolic compounds responsible for the antimicrobial activity.
Stage b) Milling of leaves and stems: the plant material is pulverized with the help of a mill and stored in black plastic bags in a dry and dark place, since the polyphenolic compounds are susceptible to light.
Stage c) Obtaining ethanolic extracts: for the obtaining of ethanolic extracts from governor Larrea tridentata and hojaén Flourensia cernua a w / v ratio of 1: 4-l: 10 mg / mL was used, preferably a ratio of 1: 4 mg / mL , the extraction is carried out in glass containers or containers that prevent the passage of light, to avoid the oxidation of the phenolic compounds, to which 100-200 g of leaves and stems, preferably 150 g of leaves, are added. and stems and 500-2000 mL, preferably 600 mL of 70% ethanol. A reflux system was prepared at a temperature between 50-60 ° C, preferably 60 ° C, with constant stirring for a time of 1-7 hours,
preferably 7 hours, with the aid of a magnetic stirrer. Finally, the ethanolic extracts of hojaén Flourensia cernua and Governor Larrea tridentata were obtained, and the solvent was removed with the help of a rotary evaporator, concentrating the polyphenolic compounds.
In order to formulate the composition of the ethanolic extracts of Governor Larrea tridentata and Hojasén Flourensia cernua and corroborate their effect to control and / or inhibit the growth of the phytopathogenic microorganisms studied in the present invention, the following tests were carried out:
Example 1. Quantification of hydrolysable tannins equivalent to gallic acid by the Folin-Ciocalteu technique.
First a standard curve of hydrolysable tannins was made in the following way, a solution of gallic acid was used at a concentration of 500 ppm, as shown in Figure 4. In test tubes marked 0 to 5, 0.80 was added. , 160, 240, 320 and 400 μ? _. of gallic acid stock solution respectively, then to the same tubes from 0 to 5 were added 400, 320, 240, 1 0, 80 and 0 xL of distilled water, to obtain a final volume of 400 μL. Then 400 xL of the Folin-Ciocalteu reagent was added, the tubes were shaken and allowed to stand for 5 minutes, 400 x 0.01 M Na2CO3 was added, the tubes were shaken and allowed to stand for 5 minutes. Finally, 2.5 mL of distilled water was added to each tube and then read at a wavelength of 725 nm in a UV / Visible thickness. The absorbances obtained were analyzed by simple linear regression and the obtained equation was used to quantify the hydrolysable tannins. This test was carried out in order to determine the amount of tannins
hydrolysable in the ethanolic extract of governor Larrea tridentata (Figure 5) and the ethanolic extract of hojaén Flourensia cernua (Figure 7) separately. The test was carried out in triplicate in the following manner, first 400 μL of the ethanolic extract sample was placed in a test tube, 400 xL of the commercial reagent Folin-Ciocalteu was added, agitated and left to rest for 5 minutes. Subsequently, it was diluted with 2.5 mL of distilled water and read in a UV / Visible spectrum at 725 nm (Varian 50 Bio). The obtained readings were analyzed by the equation obtained in the corresponding standard curve.
Example 2. Quantification of condensed tannins equivalent to catechin by the HCl-butanol technique.
The standard curve of condensed tannins (Figure 6) was made with a standard catechin solution at a concentration of 1.0 g / L. Five test tubes from 0 to 4 were labeled by adding to each tube 0, 125, 250, 375 and 500 respectively of catechin stock solution, then 500, 375, 250, 25 and 0 of distilled water were added to each tube, to obtain a final volume of 500 μ ?. Then 3 mL of HCl-butanol (1: 9) and 0.1 mL of ferric reagent were added. The tubes were covered with plugs to which they were placed a plastic packing, to avoid the evaporation of butanol and they were subjected to a heating in a water bath at a temperature of 100 ° C for 1 hour. Subsequently they were allowed to cool to room temperature and were read in a UV / Visible spectrum (They vary 50 Bio) at a wavelength of 460 nm. The absorbances obtained were analyzed by simple linear regression and the equation obtained was used to quantify the condensed tannins equivalent to catechin. In this test the amount of condensed tannins in the ethanol extract of Governor Larrea tridentata (Figure 5) and the
Ethanolic extract of leaves Flourensia cernua (Figure 7), separately. The test was performed in triplicate in the following way, in each test tube 0.5 mL of the sample or the ethanolic extract was placed, 3 mL of HCl-butanol (1: 9) and 0.1 mL of ferric reagent were added. The tubes were covered with plugs to which plastic containers were placed, to avoid the evaporation of ethanol. All the tubes were subjected to heating in a water bath at a temperature of 100 ° C for 1 hour. After the time elapsed, they were allowed to cool and read at a wavelength of 460 nm in a UV / Visible spectrum (They vary 50 Bio). The amount of condensed tannins equivalent to catechin in the samples was calculated by the equation obtained in the standard curve. Example 3. In vitro antifungal activity of the ethanolic extracts of Governor Larrea tridentata and Hojasén Flourensia cernua against phytopathogenic soil and foliar microorganisms.
In this test, the antifungal activity of the mixture or composition of ethanolic extracts against phytopathogenic microorganisms of soil of the genus Fusarium sp., Rhizoctonia sp., Sclerotinia sp. and Sclerotium sp. (Figure 1) and foliar phytopathogenic microorganisms such as Alternaria sp., Colletotrichum sp., Cercospora sp. and Pestalotia sp. (Figure 2). The technique of the poisoned medium was used, using PDA papa-dextrose agar as a culture medium, where similar concentrations of the ethanolic extract of governor Larrea tridentata and hojaén Flourensia cernua were evaluated separately, which correspond to 0, 200, 300, 330, 700, 920 and 1000 mg / L. A 5 mm segment of the microorganism is cut, obtained from the margin of a colony with vigorous growth. The 5 mm explant is placed in the central part of each of the petri dishes containing the poisoned medium in the aforementioned concentrations of ethanolic extract of
Governor Larrea tridentata and Hojasén Flourensia cernua, separately, with their corresponding witness. The inoculated boxes are incubated at a temperature of 26 ° C until the control treatment fills three quarters of the petri dish and the mycelial growth of the treatments is subsequently measured. The mycelial growth of the control (0 mg / L) is considered as 100% of the growth of the fungus at these conditions. The measurements are made with a digital vernier taking two cross readings of each petri dish, the average of which is the result of a repetition.
Example 4. Antibacterial activity // vitro of the ethanolic extracts of governor Larrea tridentata and hojaén Flourensia cernua in phytopathogenic bacteria. In this study, the antibacterial activity of the mixture or composition of ethanolic extracts against bacteria of the genus Clavibacter sp. and Ralstonia sp., whose results are shown in Figure 3. Nutrient agar was used as a culture medium, to which different concentrations of ethanol extracts from governor Larrea tridentata and hojaén Flourensia cernua were added separately, which correspond to 0, 200, 300, 330, 700, 920 and 1000 mg / L, with four repetitions per treatment. First, 100 of the bacterial suspension adjusted to 1 x 10'6 CFU are dispersed in the culture medium and incubated at 28 ° C for 24-72 hours. The number of colonies is quantified and the percentage of inhibition is estimated, considering the number of control colonies (CFU) (0 mg / L) as 0% inhibition.
Subsequently, the required volume of the ethanolic extract of Governor Larrea tridentata and Hojasén Flourensia cernua is calculated separately, to perform the mixture or composition, based on the concentrations that were most effective
antimicrobial, by the following formula CiVi = C2V2; where Ci = concentration of total tannins in mg / L of the extract, C2 = concentration to be analyzed in mg / L, V2 = Volume of the product to be analyzed and / or formulated, whose concentrations were the following for each of the phytopathogenic microorganisms that The following are mentioned: 200 mg / L for Clavibacter michiganensis sp., Rhizoctonia sp. and Sclerotinia sp. respectively, 300 mg / L for Colletotrichum sp. and Sclerotium sp. respectively, 330 mg / L for Pestalotia sp., 700 mg / L for Alternaria sp., 920 mg / L for Fusarium sp. and 1000 mg / L for Ralstonia sp.
Claims (31)
1. Process and obtaining a composition containing a mixture of ethanol extracts from governor Larrea tridentata and hojaén Flourensia cernua with antimicrobial activity, characterized in that it comprises the following stages: Stage a) Dehydration of leaves and stems, Stage b) Milling of leaves and stems and Stage c) Obtaining ethanol extracts.
2. Process and obtaining a composition containing a mixture of ethanol extracts from governor Larrea tridentata and hojaén Flourensia cernua with antimicrobial activity, according to claim 1, characterized in that Stage a), comprises the dehydration of leaves and stems of Larrea tridentata and leavesleader Flourensia cernua outdoors and in stove.
3. Process and obtaining a composition containing a mixture of ethanol extracts of governor Larrea tridentata and hojaén Flourensia cernua with antimicrobial activity, according to claim 2, characterized in that in the dehydration to the weather the plant material is exposed to the sunlight of 45- 60 hours, preferably 48 hours.
4. Process and obtaining a composition containing a mixture of ethanol extracts of governor Larrea tridentata and hojaén Flourensia cernua with antimicrobial activity, according to claim 2, characterized in that in the dehydration in the oven the plant material is dehydrated for the second time temperatures of 50-60 ° C, preferably at 60 ° C within a time range of 45-60 hours, preferably 48 hours.
5. Process and obtaining a composition containing a mixture of ethanol extracts from governor Larrea tridentata and hojaén Flourensia cernua with antimicrobial activity, according to claim 1, characterized in that Stage b), comprises spraying the plant material with a mill and storing it.
6. Process and obtaining a composition containing a mixture of ethanol extracts from governor Larrea tridentata and hojaén Flourensia cernua with antimicrobial activity, according to claim 5, characterized in that the storage is carried out in black plastic bags in a dry and dark place, and that polyphenolic compounds are susceptible to light.
7. Process and obtaining a composition containing a mixture of ethanol extracts of governor Larrea tridentata and hojaén Flourensia cernua with antimicrobial activity, according to claim 1, characterized in that Stage c), comprises the following steps: Stage el) Use of a ratio w / v plant material / solvent, Stage c2) Extraction process with reflux system and Stage c3) Removal of solvent.
8. Process and obtaining a composition containing a mixture of ethanol extracts of governor Larrea tridentata and hojaén Flourensia cernua with antimicrobial activity, according to claim 7, characterized in that step el), comprises the use of the ratio 1: 4-1: 10 mg / mL, preferably a ratio of 1: 4 mg / mL.
9. Process and obtaining a composition containing a mixture of ethanol extracts of governor Larrea tridentata and hojaén Flourensia cernua with antimicrobial activity, according to claim 7, characterized in that Stage c2), is carried out in glass or metal containers that prevent the passage of light, to avoid the oxidation of phenolic compounds.
10. Process and obtaining a composition containing a mixture of ethanol extracts from governor Larrea tridentata and hojaén Flourensia cernua with antimicrobial activity, according to claim 9, characterized in that 100-200 g of leaves and stems are added to the glass or metal containers. , preferably 150 g of leaves and stems and 500-2000 mL, preferably 600 mL of 70% ethanol.
1 1. Process and obtaining a composition containing a mixture of ethanol extracts from governor Larrea tridentata and hojaén Flourensia cernua with antimicrobial activity, according to claim 9, characterized in that Stage c2) was carried out at a temperature between 50-60 0 C, preferably 60 0 C, with stirring for 1-7 hours, preferably 7 hours.
12. Process and obtaining a composition containing a mixture of ethanol extracts from governor Larrea tridentata and hojaén Flourensia cernua with antimicrobial activity, according to claim 7, characterized in that Stage c3) Removal of solvent, comprises the elimination of the solvent (ethanol) from the ethanol extracts from governor Larrea tridentata and hojaén Flourensia cernua with the help of a rotavapor, concentrating the polyphenolic compounds.
13. The use of the composition containing a mixture of ethanol extracts of governor Larrea tridentata and hojaén Flourensia cernua with antimicrobial activity, characterized in that it presents tannins to control and / or inhibit the growth of phytopathogenic microorganisms. Formulation of the composition of ethanol extracts.
14. The use of the composition containing a mixture of ethanol extracts of governor Larrea tridentata and hojaén Flourensia cernua with antimicrobial activity, according to claim 13, characterized in that the ethanolic extract of governor Larrea tridentata and hojasén Flourensia cernua comprises hydrolysable tannins, condensed tannins and tannins totals
15. The use of the composition containing a mixture of ethanol extracts of governor Larrea tridentata and hojaén Flourensia cernua with antimicrobial activity, according to claim 14, characterized in that the concentration of hydrolysable tannins and condensed tannins correspond to 15,708 mg / L and 1,940 mg / L, respectively, of the ethanolic extract of governor Larrea tridentata.
16. The use of the composition containing a mixture of ethanol extracts of governor Larrea tridentata and hojaén Flourensia cernua with antimicrobial activity, according to claim 14, characterized in that the concentration of hydrolysable tannins and condensed tannins correspond to 1,150 mg / L and 2,683 mg / L, respectively, of the ethanolic extract of leaves Flourensia cernua.
17. The use of the composition containing a mixture of ethanol extracts from governor Larrea tridentata and hojaén Flourensia cernua with activity antimicrobial, according to claim 14, characterized in that the concentration of total tannins corresponds to 17,648 mg / L of the ethanolic extract of Governor Larrea tridentata.
18. The use of the composition containing a mixture of ethanol extracts of governor Larrea tridentata and hojaén Flourensia cernua with antimicrobial activity, according to claim 14, characterized in that the amount of total tannins corresponds to 3.833 mg / L of the ethanol extract of leaves Flourensia cernua.
19. The use of the composition containing a mixture of ethanol extracts of governor Larrea tridentata and hojaén Flourensia cernua with antimicrobial activity, according to claim 14, characterized in that the total tannin content of the composition is 21,481 mg / L.
20. The use of the composition containing a mixture of ethanol extracts of governor Larrea tridentata and hojaén Flourensia cernua with antimicrobial activity, according to claim 13, characterized in that the composition controls and / or inhibits the growth of soil phytopathogenic fungi, foliar phytopathogenic fungi and phytopathogenic bacteria in agricultural crops.
21. The use of the composition containing a mixture of ethanol extracts of governor Larrea tridentata and hojaén Flourensia cernua with antimicrobial activity, according to claim 20, characterized in that the phytopathogenic soil fungi correspond to Fusarium sp. in Chile, Rhizoctonia sp. in potato, Sclerotinia sp. in lettuce and / or cabbage and Sclerotium sp. in garlic and onion.
22. The use of the composition of ethanol extracts of governor Larrea tridentata and hojaén Flourensia cernua with antimicrobial activity, according to claim 21, characterized in that the concentration of 200 mg / L, inhibits Rhizoctonia sp. and Sclerotinia sp .; 300 mg / L inhibits Sclerotium sp. and 920 mg / L inhibits Fusarium sp., showing a 100% inhibition in its growth.
23. The use of the composition containing a mixture of ethanol extracts of governor Larrea tridentata and hojaén Flourensia cernua with antimicrobial activity, according to claim 20, characterized in that the phytopathogenic fungi correspond to Alternaria sp. in tomato, Colletotrichum sp. in papaya, Cercospora sp. in soybeans and Pestalotia sp. in mango.
24. The use of the composition of ethanol extracts of governor Larrea tridentata and hojaén Flourensia cernua with antimicrobial activity, according to claim 23, characterized in that the concentration of 300 mg / L inhibits Colletotrichum sp., 330 mg / L inhibits Pestalotia sp. and 700 mg / L inhibits Alternaria sp. which showed a 100% inhibition in their growth.
25. The use of the composition containing a mixture of ethanol extracts of governor Larrea tridentata and hojaén Flourensia cernua with antimicrobial activity, according to claim 20, characterized in that the phytopathogenic bacteria correspond to Clavibacter michiganensis sp. in tomato and Ralstonia sp. in papa
26. The use of the composition of ethanol extracts of governor Larrea tridentata and hojaén Flourensia cernua with antimicrobial activity, according to claim 25, characterized in that the concentration of 200 mg / L inhibits Clavibacter michiganensis sp. and 1000 mg L inhibits Ralstonia sp. presenting a 100% inhibition in its growth.
27. The composition of ethanol extracts of governor Larrea tridentata and hojaén Flourensia cernua with antimicrobial activity, characterized essentially because the composition is constituted by an ethanolic extract of governor Larrea tridentata and an ethanolic extract of hojaén Flourensia cernua.
28. The composition of ethanol extracts of governor Larrea tridentata and hojaén Flourensia cernua with antimicrobial activity, according to claim 27, characterized in that the ethanol extracts are prepared with 70% ethanol.
29. The composition of ethanol extracts of governor Larrea tridentata and hojaén Flourensia cernua with antimicrobial activity, according to claim 27, characterized in that the ethanol extracts are prepared in a weight / volume ratio of 1: 4-l: 10, preferably 1: 4.
30. The composition of ethanol extracts of governor Larrea tridentata and hojaén Flourensia cernua with antimicrobial activity, according to claim 27, characterized in that to calculate the necessary volume of the ethanolic extract of governor Larrea tridentata and hojaén Flourensia cernua separately, to make the composition based on the concentrations that showed the greatest antimicrobial effectiveness, the following formula CiV) = C2V2 was used.
31. The composition of ethanol extracts of governor Larrea tridentata and hojaén Flourensia cernua with antimicrobial activity, according to claim 30, characterized in that Ci = concentration of total tannins in mg / L of the extract, C2 = concentration to be analyzed in mg / L, V2 = Volume of the product to be analyzed and / or formulate.
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| Publication number | Priority date | Publication date | Assignee | Title |
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| WO2019164388A1 (en) * | 2018-02-21 | 2019-08-29 | Osuna Sanchez Juan Genaro | Functionalised extract of larrea tridentata with biocidal activity |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| WO2019164388A1 (en) * | 2018-02-21 | 2019-08-29 | Osuna Sanchez Juan Genaro | Functionalised extract of larrea tridentata with biocidal activity |
| US11337428B2 (en) | 2018-02-21 | 2022-05-24 | Juan Genaro Osuna Sanchez | Functionalized extract of Larrea tridentata with biocidal activity |
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| MX362296B (en) | 2018-07-25 |
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