MX2012000259A - Oxidative stress resistant bifidobacteria. - Google Patents
Oxidative stress resistant bifidobacteria.Info
- Publication number
- MX2012000259A MX2012000259A MX2012000259A MX2012000259A MX2012000259A MX 2012000259 A MX2012000259 A MX 2012000259A MX 2012000259 A MX2012000259 A MX 2012000259A MX 2012000259 A MX2012000259 A MX 2012000259A MX 2012000259 A MX2012000259 A MX 2012000259A
- Authority
- MX
- Mexico
- Prior art keywords
- composition
- ncc2916
- food
- bifidobacteria
- bifidobacterium
- Prior art date
Links
- 241000186000 Bifidobacterium Species 0.000 title claims abstract description 25
- 230000036542 oxidative stress Effects 0.000 title abstract description 4
- 239000000203 mixture Substances 0.000 claims description 45
- 241000894006 Bacteria Species 0.000 claims description 22
- 239000006041 probiotic Substances 0.000 claims description 21
- 235000018291 probiotics Nutrition 0.000 claims description 21
- 241001608472 Bifidobacterium longum Species 0.000 claims description 18
- 229940009291 bifidobacterium longum Drugs 0.000 claims description 18
- 235000013305 food Nutrition 0.000 claims description 18
- 230000000529 probiotic effect Effects 0.000 claims description 14
- 235000013406 prebiotics Nutrition 0.000 claims description 9
- 239000000835 fiber Substances 0.000 claims description 6
- RFSUNEUAIZKAJO-ARQDHWQXSA-N Fructose Chemical compound OC[C@H]1O[C@](O)(CO)[C@@H](O)[C@@H]1O RFSUNEUAIZKAJO-ARQDHWQXSA-N 0.000 claims description 5
- 241000894007 species Species 0.000 claims description 5
- 230000001590 oxidative effect Effects 0.000 claims description 4
- 229930091371 Fructose Natural products 0.000 claims description 3
- 239000005715 Fructose Substances 0.000 claims description 3
- 229920001202 Inulin Polymers 0.000 claims description 3
- 241001465754 Metazoa Species 0.000 claims description 3
- JYJIGFIDKWBXDU-MNNPPOADSA-N inulin Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)OC[C@]1(OC[C@]2(OC[C@]3(OC[C@]4(OC[C@]5(OC[C@]6(OC[C@]7(OC[C@]8(OC[C@]9(OC[C@]%10(OC[C@]%11(OC[C@]%12(OC[C@]%13(OC[C@]%14(OC[C@]%15(OC[C@]%16(OC[C@]%17(OC[C@]%18(OC[C@]%19(OC[C@]%20(OC[C@]%21(OC[C@]%22(OC[C@]%23(OC[C@]%24(OC[C@]%25(OC[C@]%26(OC[C@]%27(OC[C@]%28(OC[C@]%29(OC[C@]%30(OC[C@]%31(OC[C@]%32(OC[C@]%33(OC[C@]%34(OC[C@]%35(OC[C@]%36(O[C@@H]%37[C@@H]([C@@H](O)[C@H](O)[C@@H](CO)O%37)O)[C@H]([C@H](O)[C@@H](CO)O%36)O)[C@H]([C@H](O)[C@@H](CO)O%35)O)[C@H]([C@H](O)[C@@H](CO)O%34)O)[C@H]([C@H](O)[C@@H](CO)O%33)O)[C@H]([C@H](O)[C@@H](CO)O%32)O)[C@H]([C@H](O)[C@@H](CO)O%31)O)[C@H]([C@H](O)[C@@H](CO)O%30)O)[C@H]([C@H](O)[C@@H](CO)O%29)O)[C@H]([C@H](O)[C@@H](CO)O%28)O)[C@H]([C@H](O)[C@@H](CO)O%27)O)[C@H]([C@H](O)[C@@H](CO)O%26)O)[C@H]([C@H](O)[C@@H](CO)O%25)O)[C@H]([C@H](O)[C@@H](CO)O%24)O)[C@H]([C@H](O)[C@@H](CO)O%23)O)[C@H]([C@H](O)[C@@H](CO)O%22)O)[C@H]([C@H](O)[C@@H](CO)O%21)O)[C@H]([C@H](O)[C@@H](CO)O%20)O)[C@H]([C@H](O)[C@@H](CO)O%19)O)[C@H]([C@H](O)[C@@H](CO)O%18)O)[C@H]([C@H](O)[C@@H](CO)O%17)O)[C@H]([C@H](O)[C@@H](CO)O%16)O)[C@H]([C@H](O)[C@@H](CO)O%15)O)[C@H]([C@H](O)[C@@H](CO)O%14)O)[C@H]([C@H](O)[C@@H](CO)O%13)O)[C@H]([C@H](O)[C@@H](CO)O%12)O)[C@H]([C@H](O)[C@@H](CO)O%11)O)[C@H]([C@H](O)[C@@H](CO)O%10)O)[C@H]([C@H](O)[C@@H](CO)O9)O)[C@H]([C@H](O)[C@@H](CO)O8)O)[C@H]([C@H](O)[C@@H](CO)O7)O)[C@H]([C@H](O)[C@@H](CO)O6)O)[C@H]([C@H](O)[C@@H](CO)O5)O)[C@H]([C@H](O)[C@@H](CO)O4)O)[C@H]([C@H](O)[C@@H](CO)O3)O)[C@H]([C@H](O)[C@@H](CO)O2)O)[C@@H](O)[C@H](O)[C@@H](CO)O1 JYJIGFIDKWBXDU-MNNPPOADSA-N 0.000 claims description 3
- 229940029339 inulin Drugs 0.000 claims description 3
- 229920001542 oligosaccharide Polymers 0.000 claims description 3
- 150000002482 oligosaccharides Chemical class 0.000 claims description 3
- WQZGKKKJIJFFOK-QTVWNMPRSA-N D-mannopyranose Chemical compound OC[C@H]1OC(O)[C@@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-QTVWNMPRSA-N 0.000 claims description 2
- WQZGKKKJIJFFOK-PHYPRBDBSA-N alpha-D-galactose Chemical compound OC[C@H]1O[C@H](O)[C@H](O)[C@@H](O)[C@H]1O WQZGKKKJIJFFOK-PHYPRBDBSA-N 0.000 claims description 2
- 229930182830 galactose Natural products 0.000 claims description 2
- 239000008194 pharmaceutical composition Substances 0.000 claims description 2
- MHAJPDPJQMAIIY-UHFFFAOYSA-N Hydrogen peroxide Chemical compound OO MHAJPDPJQMAIIY-UHFFFAOYSA-N 0.000 abstract description 5
- 235000018102 proteins Nutrition 0.000 description 11
- 102000004169 proteins and genes Human genes 0.000 description 11
- 108090000623 proteins and genes Proteins 0.000 description 11
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 8
- 229910052760 oxygen Inorganic materials 0.000 description 8
- 239000001301 oxygen Substances 0.000 description 8
- 239000003642 reactive oxygen metabolite Substances 0.000 description 8
- KDXKERNSBIXSRK-UHFFFAOYSA-N Lysine Natural products NCCCCC(N)C(O)=O KDXKERNSBIXSRK-UHFFFAOYSA-N 0.000 description 5
- 239000004472 Lysine Substances 0.000 description 5
- 239000003795 chemical substances by application Substances 0.000 description 5
- 239000003925 fat Substances 0.000 description 4
- 238000000034 method Methods 0.000 description 4
- LWGJTAZLEJHCPA-UHFFFAOYSA-N n-(2-chloroethyl)-n-nitrosomorpholine-4-carboxamide Chemical compound ClCCN(N=O)C(=O)N1CCOCC1 LWGJTAZLEJHCPA-UHFFFAOYSA-N 0.000 description 4
- 238000003860 storage Methods 0.000 description 4
- 230000035882 stress Effects 0.000 description 4
- 230000004083 survival effect Effects 0.000 description 4
- 108010046377 Whey Proteins Proteins 0.000 description 3
- 102000007544 Whey Proteins Human genes 0.000 description 3
- 230000008901 benefit Effects 0.000 description 3
- 150000001720 carbohydrates Chemical class 0.000 description 3
- 235000014633 carbohydrates Nutrition 0.000 description 3
- 235000013325 dietary fiber Nutrition 0.000 description 3
- 239000003995 emulsifying agent Substances 0.000 description 3
- -1 etc.) Substances 0.000 description 3
- 230000007407 health benefit Effects 0.000 description 3
- 230000007062 hydrolysis Effects 0.000 description 3
- 238000006460 hydrolysis reaction Methods 0.000 description 3
- 230000000813 microbial effect Effects 0.000 description 3
- 239000000047 product Substances 0.000 description 3
- 238000001694 spray drying Methods 0.000 description 3
- YBJHBAHKTGYVGT-ZKWXMUAHSA-N (+)-Biotin Chemical compound N1C(=O)N[C@@H]2[C@H](CCCCC(=O)O)SC[C@@H]21 YBJHBAHKTGYVGT-ZKWXMUAHSA-N 0.000 description 2
- GVJHHUAWPYXKBD-UHFFFAOYSA-N (±)-α-Tocopherol Chemical compound OC1=C(C)C(C)=C2OC(CCCC(C)CCCC(C)CCCC(C)C)(C)CCC2=C1C GVJHHUAWPYXKBD-UHFFFAOYSA-N 0.000 description 2
- 244000215068 Acacia senegal Species 0.000 description 2
- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 description 2
- 241001213452 Bifidobacterium longum NCC2705 Species 0.000 description 2
- 108090000790 Enzymes Proteins 0.000 description 2
- 102000004190 Enzymes Human genes 0.000 description 2
- 235000010469 Glycine max Nutrition 0.000 description 2
- 229920000084 Gum arabic Polymers 0.000 description 2
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 2
- GUBGYTABKSRVRQ-QKKXKWKRSA-N Lactose Natural products OC[C@H]1O[C@@H](O[C@H]2[C@H](O)[C@@H](O)C(O)O[C@@H]2CO)[C@H](O)[C@@H](O)[C@H]1O GUBGYTABKSRVRQ-QKKXKWKRSA-N 0.000 description 2
- 241000736262 Microbiota Species 0.000 description 2
- 108010011756 Milk Proteins Proteins 0.000 description 2
- 102000014171 Milk Proteins Human genes 0.000 description 2
- 240000004713 Pisum sativum Species 0.000 description 2
- 235000010582 Pisum sativum Nutrition 0.000 description 2
- 108010073771 Soybean Proteins Proteins 0.000 description 2
- 239000005862 Whey Substances 0.000 description 2
- 239000000205 acacia gum Substances 0.000 description 2
- 235000010489 acacia gum Nutrition 0.000 description 2
- 238000013459 approach Methods 0.000 description 2
- 230000001580 bacterial effect Effects 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 2
- 239000007853 buffer solution Substances 0.000 description 2
- 239000006285 cell suspension Substances 0.000 description 2
- 230000000112 colonic effect Effects 0.000 description 2
- 230000007123 defense Effects 0.000 description 2
- 239000000945 filler Substances 0.000 description 2
- OVBPIULPVIDEAO-LBPRGKRZSA-N folic acid Chemical compound C=1N=C2NC(N)=NC(=O)C2=NC=1CNC1=CC=C(C(=O)N[C@@H](CCC(O)=O)C(O)=O)C=C1 OVBPIULPVIDEAO-LBPRGKRZSA-N 0.000 description 2
- 230000036541 health Effects 0.000 description 2
- 210000000936 intestine Anatomy 0.000 description 2
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 description 2
- 239000008101 lactose Substances 0.000 description 2
- 238000011031 large-scale manufacturing process Methods 0.000 description 2
- 239000000787 lecithin Substances 0.000 description 2
- 235000010445 lecithin Nutrition 0.000 description 2
- 244000144972 livestock Species 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 239000011785 micronutrient Substances 0.000 description 2
- 235000013369 micronutrients Nutrition 0.000 description 2
- 235000021239 milk protein Nutrition 0.000 description 2
- 235000016709 nutrition Nutrition 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- LXNHXLLTXMVWPM-UHFFFAOYSA-N pyridoxine Chemical compound CC1=NC=C(CO)C(CO)=C1O LXNHXLLTXMVWPM-UHFFFAOYSA-N 0.000 description 2
- 239000002904 solvent Substances 0.000 description 2
- 229940001941 soy protein Drugs 0.000 description 2
- 239000003381 stabilizer Substances 0.000 description 2
- 239000000080 wetting agent Substances 0.000 description 2
- WRIDQFICGBMAFQ-UHFFFAOYSA-N (E)-8-Octadecenoic acid Natural products CCCCCCCCCC=CCCCCCCC(O)=O WRIDQFICGBMAFQ-UHFFFAOYSA-N 0.000 description 1
- IIZPXYDJLKNOIY-JXPKJXOSSA-N 1-palmitoyl-2-arachidonoyl-sn-glycero-3-phosphocholine Chemical compound CCCCCCCCCCCCCCCC(=O)OC[C@H](COP([O-])(=O)OCC[N+](C)(C)C)OC(=O)CCC\C=C/C\C=C/C\C=C/C\C=C/CCCCC IIZPXYDJLKNOIY-JXPKJXOSSA-N 0.000 description 1
- LQJBNNIYVWPHFW-UHFFFAOYSA-N 20:1omega9c fatty acid Natural products CCCCCCCCCCC=CCCCCCCCC(O)=O LQJBNNIYVWPHFW-UHFFFAOYSA-N 0.000 description 1
- OIZGSVFYNBZVIK-FHHHURIISA-N 3'-sialyllactose Chemical compound O1[C@@H]([C@H](O)[C@H](O)CO)[C@H](NC(=O)C)[C@@H](O)C[C@@]1(C(O)=O)O[C@@H]1[C@@H](O)[C@H](O[C@H]([C@H](O)CO)[C@H](O)[C@@H](O)C=O)O[C@H](CO)[C@@H]1O OIZGSVFYNBZVIK-FHHHURIISA-N 0.000 description 1
- QSBYPNXLFMSGKH-UHFFFAOYSA-N 9-Heptadecensaeure Natural products CCCCCCCC=CCCCCCCCC(O)=O QSBYPNXLFMSGKH-UHFFFAOYSA-N 0.000 description 1
- 229920001817 Agar Polymers 0.000 description 1
- GUBGYTABKSRVRQ-XLOQQCSPSA-N Alpha-Lactose Chemical compound O[C@@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@H]1O[C@@H]1[C@@H](CO)O[C@H](O)[C@H](O)[C@H]1O GUBGYTABKSRVRQ-XLOQQCSPSA-N 0.000 description 1
- 235000007319 Avena orientalis Nutrition 0.000 description 1
- 244000075850 Avena orientalis Species 0.000 description 1
- 241000283690 Bos taurus Species 0.000 description 1
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 1
- 241000282472 Canis lupus familiaris Species 0.000 description 1
- 241000283707 Capra Species 0.000 description 1
- 102000011632 Caseins Human genes 0.000 description 1
- 108010076119 Caseins Proteins 0.000 description 1
- 108010053835 Catalase Proteins 0.000 description 1
- 102000016938 Catalase Human genes 0.000 description 1
- 241000700198 Cavia Species 0.000 description 1
- RYGMFSIKBFXOCR-UHFFFAOYSA-N Copper Chemical compound [Cu] RYGMFSIKBFXOCR-UHFFFAOYSA-N 0.000 description 1
- AUNGANRZJHBGPY-UHFFFAOYSA-N D-Lyxoflavin Natural products OCC(O)C(O)C(O)CN1C=2C=C(C)C(C)=CC=2N=C2C1=NC(=O)NC2=O AUNGANRZJHBGPY-UHFFFAOYSA-N 0.000 description 1
- ZZZCUOFIHGPKAK-UHFFFAOYSA-N D-erythro-ascorbic acid Natural products OCC1OC(=O)C(O)=C1O ZZZCUOFIHGPKAK-UHFFFAOYSA-N 0.000 description 1
- 230000005778 DNA damage Effects 0.000 description 1
- 231100000277 DNA damage Toxicity 0.000 description 1
- 108010082495 Dietary Plant Proteins Proteins 0.000 description 1
- 101100223916 Drosophila melanogaster pea gene Proteins 0.000 description 1
- 102000002322 Egg Proteins Human genes 0.000 description 1
- 108010000912 Egg Proteins Proteins 0.000 description 1
- 241000283086 Equidae Species 0.000 description 1
- 101100202725 Escherichia coli (strain K12) secA gene Proteins 0.000 description 1
- 239000001422 FEMA 4092 Substances 0.000 description 1
- 241000282326 Felis catus Species 0.000 description 1
- 108010010803 Gelatin Proteins 0.000 description 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
- 244000068988 Glycine max Species 0.000 description 1
- 229920002907 Guar gum Polymers 0.000 description 1
- 206010020751 Hypersensitivity Diseases 0.000 description 1
- 229920001732 Lignosulfonate Polymers 0.000 description 1
- FYYHWMGAXLPEAU-UHFFFAOYSA-N Magnesium Chemical compound [Mg] FYYHWMGAXLPEAU-UHFFFAOYSA-N 0.000 description 1
- 229920002774 Maltodextrin Polymers 0.000 description 1
- 108010070551 Meat Proteins Proteins 0.000 description 1
- 201000010859 Milk allergy Diseases 0.000 description 1
- 229920000881 Modified starch Polymers 0.000 description 1
- OVBPIULPVIDEAO-UHFFFAOYSA-N N-Pteroyl-L-glutaminsaeure Natural products C=1N=C2NC(N)=NC(=O)C2=NC=1CNC1=CC=C(C(=O)NC(CCC(O)=O)C(O)=O)C=C1 OVBPIULPVIDEAO-UHFFFAOYSA-N 0.000 description 1
- 108030000527 NADH peroxidases Proteins 0.000 description 1
- PVNIIMVLHYAWGP-UHFFFAOYSA-N Niacin Chemical compound OC(=O)C1=CC=CN=C1 PVNIIMVLHYAWGP-UHFFFAOYSA-N 0.000 description 1
- 239000005642 Oleic acid Substances 0.000 description 1
- ZQPPMHVWECSIRJ-UHFFFAOYSA-N Oleic acid Natural products CCCCCCCCC=CCCCCCCCC(O)=O ZQPPMHVWECSIRJ-UHFFFAOYSA-N 0.000 description 1
- 241000283973 Oryctolagus cuniculus Species 0.000 description 1
- 240000007594 Oryza sativa Species 0.000 description 1
- 235000007164 Oryza sativa Nutrition 0.000 description 1
- 108090000854 Oxidoreductases Proteins 0.000 description 1
- 102000004316 Oxidoreductases Human genes 0.000 description 1
- 108010084695 Pea Proteins Proteins 0.000 description 1
- 241001494479 Pecora Species 0.000 description 1
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 1
- 108091007187 Reductases Proteins 0.000 description 1
- AUNGANRZJHBGPY-SCRDCRAPSA-N Riboflavin Chemical compound OC[C@@H](O)[C@@H](O)[C@@H](O)CN1C=2C=C(C)C(C)=CC=2N=C2C1=NC(=O)NC2=O AUNGANRZJHBGPY-SCRDCRAPSA-N 0.000 description 1
- 241000831652 Salinivibrio sharmensis Species 0.000 description 1
- BUGBHKTXTAQXES-UHFFFAOYSA-N Selenium Chemical compound [Se] BUGBHKTXTAQXES-UHFFFAOYSA-N 0.000 description 1
- 229920002472 Starch Polymers 0.000 description 1
- 229930006000 Sucrose Natural products 0.000 description 1
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 1
- 241000282887 Suidae Species 0.000 description 1
- 235000019486 Sunflower oil Nutrition 0.000 description 1
- OUUQCZGPVNCOIJ-UHFFFAOYSA-M Superoxide Chemical compound [O-][O] OUUQCZGPVNCOIJ-UHFFFAOYSA-M 0.000 description 1
- 235000021307 Triticum Nutrition 0.000 description 1
- 244000098338 Triticum aestivum Species 0.000 description 1
- 229920001938 Vegetable gum Polymers 0.000 description 1
- 229930003451 Vitamin B1 Natural products 0.000 description 1
- 229930003779 Vitamin B12 Natural products 0.000 description 1
- 229930003471 Vitamin B2 Natural products 0.000 description 1
- 229930003268 Vitamin C Natural products 0.000 description 1
- 229930003316 Vitamin D Natural products 0.000 description 1
- QYSXJUFSXHHAJI-XFEUOLMDSA-N Vitamin D3 Natural products C1(/[C@@H]2CC[C@@H]([C@]2(CCC1)C)[C@H](C)CCCC(C)C)=C/C=C1\C[C@@H](O)CCC1=C QYSXJUFSXHHAJI-XFEUOLMDSA-N 0.000 description 1
- 229930003427 Vitamin E Natural products 0.000 description 1
- 240000008042 Zea mays Species 0.000 description 1
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 description 1
- 235000002017 Zea mays subsp mays Nutrition 0.000 description 1
- HCHKCACWOHOZIP-UHFFFAOYSA-N Zinc Chemical compound [Zn] HCHKCACWOHOZIP-UHFFFAOYSA-N 0.000 description 1
- 239000000654 additive Substances 0.000 description 1
- 239000002671 adjuvant Substances 0.000 description 1
- 239000003463 adsorbent Substances 0.000 description 1
- 241001148470 aerobic bacillus Species 0.000 description 1
- 239000008272 agar Substances 0.000 description 1
- 230000007815 allergy Effects 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 235000020244 animal milk Nutrition 0.000 description 1
- 235000021120 animal protein Nutrition 0.000 description 1
- 239000004599 antimicrobial Substances 0.000 description 1
- 239000003963 antioxidant agent Substances 0.000 description 1
- 235000006708 antioxidants Nutrition 0.000 description 1
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 1
- 235000013361 beverage Nutrition 0.000 description 1
- 239000011230 binding agent Substances 0.000 description 1
- 235000020958 biotin Nutrition 0.000 description 1
- 239000011616 biotin Substances 0.000 description 1
- 229960002685 biotin Drugs 0.000 description 1
- 230000000903 blocking effect Effects 0.000 description 1
- 239000011575 calcium Substances 0.000 description 1
- 229910052791 calcium Inorganic materials 0.000 description 1
- 239000000828 canola oil Substances 0.000 description 1
- 235000019519 canola oil Nutrition 0.000 description 1
- 239000005018 casein Substances 0.000 description 1
- BECPQYXYKAMYBN-UHFFFAOYSA-N casein, tech. Chemical compound NCCCCC(C(O)=O)N=C(O)C(CC(O)=O)N=C(O)C(CCC(O)=N)N=C(O)C(CC(C)C)N=C(O)C(CCC(O)=O)N=C(O)C(CC(O)=O)N=C(O)C(CCC(O)=O)N=C(O)C(C(C)O)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=O)N=C(O)C(CCC(O)=O)N=C(O)C(COP(O)(O)=O)N=C(O)C(CCC(O)=N)N=C(O)C(N)CC1=CC=CC=C1 BECPQYXYKAMYBN-UHFFFAOYSA-N 0.000 description 1
- 235000021240 caseins Nutrition 0.000 description 1
- 230000003833 cell viability Effects 0.000 description 1
- 238000000576 coating method Methods 0.000 description 1
- AGVAZMGAQJOSFJ-WZHZPDAFSA-M cobalt(2+);[(2r,3s,4r,5s)-5-(5,6-dimethylbenzimidazol-1-yl)-4-hydroxy-2-(hydroxymethyl)oxolan-3-yl] [(2r)-1-[3-[(1r,2r,3r,4z,7s,9z,12s,13s,14z,17s,18s,19r)-2,13,18-tris(2-amino-2-oxoethyl)-7,12,17-tris(3-amino-3-oxopropyl)-3,5,8,8,13,15,18,19-octamethyl-2 Chemical compound [Co+2].N#[C-].[N-]([C@@H]1[C@H](CC(N)=O)[C@@]2(C)CCC(=O)NC[C@@H](C)OP(O)(=O)O[C@H]3[C@H]([C@H](O[C@@H]3CO)N3C4=CC(C)=C(C)C=C4N=C3)O)\C2=C(C)/C([C@H](C\2(C)C)CCC(N)=O)=N/C/2=C\C([C@H]([C@@]/2(CC(N)=O)C)CCC(N)=O)=N\C\2=C(C)/C2=N[C@]1(C)[C@@](C)(CC(N)=O)[C@@H]2CCC(N)=O AGVAZMGAQJOSFJ-WZHZPDAFSA-M 0.000 description 1
- 239000003086 colorant Substances 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 239000010949 copper Substances 0.000 description 1
- 229910052802 copper Inorganic materials 0.000 description 1
- 235000005822 corn Nutrition 0.000 description 1
- 235000005687 corn oil Nutrition 0.000 description 1
- 239000002285 corn oil Substances 0.000 description 1
- 230000006378 damage Effects 0.000 description 1
- 235000005911 diet Nutrition 0.000 description 1
- 230000000378 dietary effect Effects 0.000 description 1
- 235000021245 dietary protein Nutrition 0.000 description 1
- 235000015872 dietary supplement Nutrition 0.000 description 1
- 230000029087 digestion Effects 0.000 description 1
- 239000003085 diluting agent Substances 0.000 description 1
- 239000002270 dispersing agent Substances 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 239000008393 encapsulating agent Substances 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000000855 fermentation Methods 0.000 description 1
- 230000004151 fermentation Effects 0.000 description 1
- 239000012467 final product Substances 0.000 description 1
- 239000000796 flavoring agent Substances 0.000 description 1
- 229960000304 folic acid Drugs 0.000 description 1
- 235000019152 folic acid Nutrition 0.000 description 1
- 239000011724 folic acid Substances 0.000 description 1
- 235000013355 food flavoring agent Nutrition 0.000 description 1
- 239000012520 frozen sample Substances 0.000 description 1
- 230000006870 function Effects 0.000 description 1
- 235000021255 galacto-oligosaccharides Nutrition 0.000 description 1
- 150000003271 galactooligosaccharides Chemical class 0.000 description 1
- WIGCFUFOHFEKBI-UHFFFAOYSA-N gamma-tocopherol Natural products CC(C)CCCC(C)CCCC(C)CCCC1CCC2C(C)C(O)C(C)C(C)C2O1 WIGCFUFOHFEKBI-UHFFFAOYSA-N 0.000 description 1
- 210000001035 gastrointestinal tract Anatomy 0.000 description 1
- 239000000499 gel Substances 0.000 description 1
- 239000008273 gelatin Substances 0.000 description 1
- 229920000159 gelatin Polymers 0.000 description 1
- 235000019322 gelatine Nutrition 0.000 description 1
- 235000011852 gelatine desserts Nutrition 0.000 description 1
- 239000003349 gelling agent Substances 0.000 description 1
- 230000002068 genetic effect Effects 0.000 description 1
- 239000008103 glucose Substances 0.000 description 1
- 230000012010 growth Effects 0.000 description 1
- 239000001963 growth medium Substances 0.000 description 1
- 239000000665 guar gum Substances 0.000 description 1
- 235000010417 guar gum Nutrition 0.000 description 1
- 229960002154 guar gum Drugs 0.000 description 1
- 239000000416 hydrocolloid Substances 0.000 description 1
- 230000003301 hydrolyzing effect Effects 0.000 description 1
- 150000002432 hydroperoxides Chemical class 0.000 description 1
- TUJKJAMUKRIRHC-UHFFFAOYSA-N hydroxyl Chemical compound [OH] TUJKJAMUKRIRHC-UHFFFAOYSA-N 0.000 description 1
- QWPPOHNGKGFGJK-UHFFFAOYSA-N hypochlorous acid Chemical compound ClO QWPPOHNGKGFGJK-UHFFFAOYSA-N 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 229910052500 inorganic mineral Inorganic materials 0.000 description 1
- PNDPGZBMCMUPRI-UHFFFAOYSA-N iodine Chemical compound II PNDPGZBMCMUPRI-UHFFFAOYSA-N 0.000 description 1
- 229910052742 iron Inorganic materials 0.000 description 1
- 238000002955 isolation Methods 0.000 description 1
- QXJSBBXBKPUZAA-UHFFFAOYSA-N isooleic acid Natural products CCCCCCCC=CCCCCCCCCC(O)=O QXJSBBXBKPUZAA-UHFFFAOYSA-N 0.000 description 1
- 239000004310 lactic acid Substances 0.000 description 1
- 235000014655 lactic acid Nutrition 0.000 description 1
- 239000008141 laxative Substances 0.000 description 1
- 229940125722 laxative agent Drugs 0.000 description 1
- 229940067606 lecithin Drugs 0.000 description 1
- 150000002632 lipids Chemical class 0.000 description 1
- 239000000314 lubricant Substances 0.000 description 1
- 239000011777 magnesium Substances 0.000 description 1
- 229910052749 magnesium Inorganic materials 0.000 description 1
- 239000011159 matrix material Substances 0.000 description 1
- 239000011707 mineral Substances 0.000 description 1
- 235000010755 mineral Nutrition 0.000 description 1
- 235000019426 modified starch Nutrition 0.000 description 1
- 229960003512 nicotinic acid Drugs 0.000 description 1
- 235000001968 nicotinic acid Nutrition 0.000 description 1
- 239000011664 nicotinic acid Substances 0.000 description 1
- 239000002417 nutraceutical Substances 0.000 description 1
- 235000021436 nutraceutical agent Nutrition 0.000 description 1
- 239000003921 oil Substances 0.000 description 1
- 235000019198 oils Nutrition 0.000 description 1
- ZQPPMHVWECSIRJ-KTKRTIGZSA-N oleic acid Chemical compound CCCCCCCC\C=C/CCCCCCCC(O)=O ZQPPMHVWECSIRJ-KTKRTIGZSA-N 0.000 description 1
- 230000003204 osmotic effect Effects 0.000 description 1
- 230000003647 oxidation Effects 0.000 description 1
- 238000007254 oxidation reaction Methods 0.000 description 1
- 238000004806 packaging method and process Methods 0.000 description 1
- 238000007911 parenteral administration Methods 0.000 description 1
- 235000019702 pea protein Nutrition 0.000 description 1
- 239000001814 pectin Substances 0.000 description 1
- 235000010987 pectin Nutrition 0.000 description 1
- 229920001277 pectin Polymers 0.000 description 1
- 239000008188 pellet Substances 0.000 description 1
- CMFNMSMUKZHDEY-UHFFFAOYSA-N peroxynitrous acid Chemical compound OON=O CMFNMSMUKZHDEY-UHFFFAOYSA-N 0.000 description 1
- 239000000546 pharmaceutical excipient Substances 0.000 description 1
- 239000011574 phosphorus Substances 0.000 description 1
- 229910052698 phosphorus Inorganic materials 0.000 description 1
- 230000035479 physiological effects, processes and functions Effects 0.000 description 1
- 229920001515 polyalkylene glycol Polymers 0.000 description 1
- 244000144977 poultry Species 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 239000003755 preservative agent Substances 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- 239000003223 protective agent Substances 0.000 description 1
- 230000001681 protective effect Effects 0.000 description 1
- 239000003531 protein hydrolysate Substances 0.000 description 1
- 230000006318 protein oxidation Effects 0.000 description 1
- RADKZDMFGJYCBB-UHFFFAOYSA-N pyridoxal hydrochloride Natural products CC1=NC=C(CO)C(C=O)=C1O RADKZDMFGJYCBB-UHFFFAOYSA-N 0.000 description 1
- 229960002477 riboflavin Drugs 0.000 description 1
- 235000009566 rice Nutrition 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 229910052711 selenium Inorganic materials 0.000 description 1
- 239000011669 selenium Substances 0.000 description 1
- 235000011649 selenium Nutrition 0.000 description 1
- 210000002966 serum Anatomy 0.000 description 1
- 210000000813 small intestine Anatomy 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 239000008107 starch Substances 0.000 description 1
- 235000019698 starch Nutrition 0.000 description 1
- 239000007858 starting material Substances 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 239000005720 sucrose Substances 0.000 description 1
- 235000000346 sugar Nutrition 0.000 description 1
- 150000008163 sugars Chemical class 0.000 description 1
- 239000002600 sunflower oil Substances 0.000 description 1
- 239000004094 surface-active agent Substances 0.000 description 1
- 239000006188 syrup Substances 0.000 description 1
- 235000020357 syrup Nutrition 0.000 description 1
- 239000000454 talc Substances 0.000 description 1
- 229910052623 talc Inorganic materials 0.000 description 1
- 235000012222 talc Nutrition 0.000 description 1
- 239000006068 taste-masking agent Substances 0.000 description 1
- 229960003495 thiamine Drugs 0.000 description 1
- DPJRMOMPQZCRJU-UHFFFAOYSA-M thiamine hydrochloride Chemical compound Cl.[Cl-].CC1=C(CCO)SC=[N+]1CC1=CN=C(C)N=C1N DPJRMOMPQZCRJU-UHFFFAOYSA-M 0.000 description 1
- 238000011200 topical administration Methods 0.000 description 1
- 231100000331 toxic Toxicity 0.000 description 1
- 230000002588 toxic effect Effects 0.000 description 1
- 235000013619 trace mineral Nutrition 0.000 description 1
- 239000011573 trace mineral Substances 0.000 description 1
- 230000004614 tumor growth Effects 0.000 description 1
- 235000015112 vegetable and seed oil Nutrition 0.000 description 1
- 239000008158 vegetable oil Substances 0.000 description 1
- 239000003981 vehicle Substances 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- 235000010374 vitamin B1 Nutrition 0.000 description 1
- 239000011691 vitamin B1 Substances 0.000 description 1
- 235000019163 vitamin B12 Nutrition 0.000 description 1
- 239000011715 vitamin B12 Substances 0.000 description 1
- 235000019164 vitamin B2 Nutrition 0.000 description 1
- 239000011716 vitamin B2 Substances 0.000 description 1
- 235000019158 vitamin B6 Nutrition 0.000 description 1
- 239000011726 vitamin B6 Substances 0.000 description 1
- 235000019154 vitamin C Nutrition 0.000 description 1
- 239000011718 vitamin C Substances 0.000 description 1
- 235000019166 vitamin D Nutrition 0.000 description 1
- 239000011710 vitamin D Substances 0.000 description 1
- 150000003710 vitamin D derivatives Chemical class 0.000 description 1
- 235000019165 vitamin E Nutrition 0.000 description 1
- 239000011709 vitamin E Substances 0.000 description 1
- 229940046009 vitamin E Drugs 0.000 description 1
- 229940011671 vitamin b6 Drugs 0.000 description 1
- 229940046008 vitamin d Drugs 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
- 239000001993 wax Substances 0.000 description 1
- 230000037221 weight management Effects 0.000 description 1
- 230000036642 wellbeing Effects 0.000 description 1
- 235000021119 whey protein Nutrition 0.000 description 1
- 239000011701 zinc Substances 0.000 description 1
- 229910052725 zinc Inorganic materials 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/16—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions
- A23K10/18—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions of live microorganisms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/135—Bacteria or derivatives thereof, e.g. probiotics
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P1/00—Drugs for disorders of the alimentary tract or the digestive system
- A61P1/14—Prodigestives, e.g. acids, enzymes, appetite stimulants, antidyspeptics, tonics, antiflatulents
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P3/00—Drugs for disorders of the metabolism
- A61P3/02—Nutrients, e.g. vitamins, minerals
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P3/00—Drugs for disorders of the metabolism
- A61P3/04—Anorexiants; Antiobesity agents
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P37/00—Drugs for immunological or allergic disorders
- A61P37/08—Antiallergic agents
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
- C12N1/205—Bacterial isolates
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/36—Adaptation or attenuation of cells
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/51—Bifidobacterium
- A23V2400/533—Longum
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Biotechnology (AREA)
- Wood Science & Technology (AREA)
- Microbiology (AREA)
- Genetics & Genomics (AREA)
- Medicinal Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Biomedical Technology (AREA)
- Polymers & Plastics (AREA)
- General Engineering & Computer Science (AREA)
- Virology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Veterinary Medicine (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Pharmacology & Pharmacy (AREA)
- Animal Behavior & Ethology (AREA)
- Public Health (AREA)
- General Chemical & Material Sciences (AREA)
- Food Science & Technology (AREA)
- Nutrition Science (AREA)
- Obesity (AREA)
- Diabetes (AREA)
- Hematology (AREA)
- Animal Husbandry (AREA)
- Physiology (AREA)
- Cell Biology (AREA)
- Molecular Biology (AREA)
- Mycology (AREA)
- Child & Adolescent Psychology (AREA)
- Pulmonology (AREA)
- Immunology (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
The present invention relates in general to the field of Bifidobacteria. In particular the present invention relates to the field of Bifidobacteria with an improved resistance to oxidative stress. The inventors have identified a Bifidobacterium that exhibits for example an improved H2O2 - resistance.
Description
BIFIDOBACTERIAS RESISTANT TO OXIDATIVE STRESS
DESCRIPTIVE MEMORY
In general, the present invention relates to the field of Bifidobacteria. In particular, the present invention relates to the field of Bifidobacteria with a better resistance to H202.
Prebiotic bacteria are selected for their health-promoting properties. As probiotics exercise their benefit as living organisms ((Guarner, F. and
G. J. Schaafsma. 1998. "Probiotics". Int.J.Food Microbiol. 39: 237-238), bacteria should survive in the conditions of the gastrointestinal tract. In industrial applications, large-scale production requires the additional ability of probiotics to tolerate food processing and storage. These production steps are characterized by different stresses that compromise a good survival of the bacteria. Among them, high or low temperature, high osmotic pressure, oxidation and humidity are likely key factors.
Some species of probiotic bacteria are known in particular for having a low tolerance to temperature, oxygen or spray drying. Important examples of said probiotics with low tolerance to stress are, for example, the Bifidobacteria, in particular, Bifidobacterium longum (Simpson, PJ, Stanton C., Fitzgerald GF, and RP Ross, 2005. "Intrinsic tolerance of Bifidobacterium species to heat and oxygen. and survival following spray drying and storage "[Intrinsic tolerance of Bifidobacteria species to heat and oxygen and survival after spray drying and storage] J.Appl.Microbiol.99: 493-501).
In particular, the anaerobic nature of bifidobacteria requires adapted fermentation conditions to facilitate large-scale production and additional effort is usually required to preserve cell viability during processing and storage.
Extreme differences in oxygen tolerance occur throughout the bacterial kingdom. Aerobic bacteria have created evolved defense systems to protect against damage caused by reactive oxygen species (ROS). However, even for these bacteria, oxygen can be toxic if such defense systems deteriorate. Primarily, three different reactive oxygen species (ROS) can be formed, depending on whether oxygen accepts one, two or three electrons to form (i) superoxide radicals (O2), (ii) hydrogen peroxide (H202) or (iii) hydroxyl radicals (OH), respectively (Imlay, JA, 2002, Advances in Microbial Physiology, Vol 46 46: 1 1 -153.). H202 causes DNA damage and protein oxidation. Bacteria can use various enzymes to eliminate these ROS, including catalases, NADH peroxidases and reductases.
To address this problem and increase the survival rate of prebiotics during food production, currently different approaches are used, including microencapsulation, addition of protective agents, oxygen-impermeable packaging and improvement of cultivation and processing conditions.
However, these approaches are complex and laborious to carry out and often require the addition of additional components that may have side effects and that may be undesirable in the final product.
Accordingly, the aim of the present invention was to overcome the problems of the prior art and to provide the art with probiotic Bifidobacteria that exhibit better resistance against oxidative stress and / or better oxygen tolerance and with a method to increase the number of viable probiotics. in a composition.
This object of the present invention is solved by a Bifidobacterium according to Claim 1, by a composition in accordance with Claim 2 and by the use according to Claim 8.
The present inventors have applied methods of natural selection and have searched for strains of Bifidobacteria with greater resistance to reactive oxygen species, such as for example H202.
A typical probiotic bacteria is Bifidobacterium longum.
The present inventors were able to identify and isolate Bifidobacterium longum NCC2916 which allowed achieving the objective of the present invention. Bifidobacterium longum NCC2916 - a derivative of Bifidobacterium longum NCC2705 obtained by natural selection - surprisingly exhibits a better tolerance to oxygen compared to the Bifidobacteria of the prior art. The strains obtained by natural selection have the advantage that they solve the objective of the present invention without having been modified by means of genetic technology.
In particular, strain B. longum NCC2916 shows greater resistance to
H202
Accordingly, one embodiment of the present invention is Bifidobacterium longum NCC2916.
Bifidobacterium longum NCC2916 has been deposited under the Budapest treaty with the CNCM; Pasteur Institute, 25; Rué du Docteur Roux; F-75724 Paris Cedex 15 as CNCM I-4053.
Bifidobacterium longum NCC2705 has been deposited under the Budapest treaty with the CNCM; Pasteur Institute, 25; Rué du Docteur Roux; F-75724 Paris Cedex 15 as CNCM 1-2618.
The present invention also relates to a composition comprising Bifidobacterium longum NCC2916.
For the purpose of the present invention, a composition is considered to contain Bifidobacterium longum NCC29 6 if it contains Bifidobacterium longum NCC2916 cells - alive or non-replicable, any bacterial fraction of Bifidobacterium longum NCC2916, any fraction of a culture comprising Bifidobacterium
NCC2916 longum, and / or the culture medium or a part thereof that was used to grow Bifidobacterium longum NCC2916.
Probiotic Bifidobacteria, when administered, confer a health benefit to the host. The health benefits of Bifidobacteria are manifold and include aid in digestion, association with a lower incidence of allergies, prevention of some forms of tumor growth and weight management. Accordingly, the composition and / or Bifidobacterium longum NCC2916 of the present invention can be used to confer these health benefits to an individual.
The composition of the present invention can be any composition but preferably it is a food product, an animal food product or a pharmaceutical composition. For example, the composition can be a nutritional composition, a nutraceutical, a beverage, a food supplement or a medicine.
Preferably, the composition is intended for human, pet or livestock, while the pets or livestock may be selected from the group consisting of dogs, cats, guinea pigs, rabbits, pigs, cattle, sheep, goats, horses and / or poultry. .
The composition may comprise at least one source of carbohydrates, at least one source of lipids and / or at least one source of proteins.
As a protein source, any suitable food protein can be used, for example, animal proteins (such as milk proteins, meat proteins and egg proteins); vegetable proteins (such as soy proteins, wheat proteins, rice proteins and pea or pea protein); mixtures of free amino acids; or combinations thereof. Of particular preference are milk proteins such as casein and whey and soy proteins. The proteins may be intact or hydrolyzed or a mixture of intact and hydrolyzed proteins. It may be of interest to supply partially hydrolyzed proteins (degree of hydrolysis between 2 and 20%), for example, for animals believed to be at risk of developing allergy to cow's milk. If hydrolyzed proteins are required, the hydrolysis process can be carried out as desired and as is known in the art. For example, a whey protein hydrolyzate can be prepared by enzymatically hydrolyzing the serum fraction in one or more steps. If the whey fraction used as the starting material is essentially free of lactose, it is observed that the protein undergoes much less lysine blockage during the hydrolysis process. The above allows the degree of lysine blocking to be reduced from about 15% by weight of total lysine to less than about 10% by weight of lysine; for example, about 7% by weight of lysine, which greatly improves the nutritional quality of the protein source.
If the composition includes a source of fat, preferably, the source of fat provides from 5% to 40% of the energy of the composition; for example from 20% to 30% of the energy. A suitable fat profile can be obtained by using a mixture of canola oil, corn oil, and sunflower oil with high oleic acid content.
Preferably, the carbohydrate source provides 40% to 80% of the energy of the composition. Any suitable carbohydrate can be used, for example,
sucrose, lactose, glucose, fructose, corn syrup solids, maltodextrins and mixtures thereof.
In addition, dietary fiber can be added if desired. The dietary fiber passes through the small intestine undigested by enzymes and functions as a natural volume and laxative agent. The food fiber may be soluble or insoluble and in general, a mixture of the two types is preferred. Suitable sources of dietary fiber include soybeans, peas, oats, pectins, guar gum, gum arabic, fructooligosaccharides, galactooligosaccharides, sialyllactose and oligosaccharides derived from animal milks. A preferred fiber mixture is a mixture of inulin with shorter chain fructooligosaccharides. Preferably, if the fiber is present, the fiber content is between 2 and 40 g / l of the composition as consumed, more preferably between 4 and 10 g / l.
The composition may also include minerals and micronutrients such as trace elements and vitamins in accordance with the recommendations of government agencies such as the USRDA.
For example, the composition may contain one or more of the following micronutrients per day: calcium, magnesium, phosphorus, iron, zinc, copper, iodine, selenium, vitamin C, vitamin B1, vitamin B6, vitamin B2, niacin, vitamin B12 , folic acid, biotin, Vitamin D, Vitamin E.
One or more food grade emulsifiers can be incorporated into the composition if desired; for example, diacetyltartaric acid esters of mono and
diglycerides, lecithin and mono and diglycerides. Salts and similarly suitable stabilizers may be included.
The composition may be intended for oral, parenteral or topical administration. For purposes of the present invention, oral application includes enteral administration.
The composition may comprise at least one other type of other food grade bacteria. "Food-grade bacteria" means bacteria that are used and that are generally considered safe for use in food. The food grade bacteria can be selected from the group consisting of lactic acid bacteria and / or bifidobacteria. Preferably they are probiotic bacteria. "Probiotic bacteria" means microbial cell preparations or components of microbial cells with beneficial effect on the health or well-being of the host. (Salminen S, Ouwehand A. Benno Y. et al "Probiotics: how should they be defined [Probiotics: how should they be defined] '
Trends Food Sci. Technol. 1999: 10 107-10).
The composition may also contain at least one prebiotic. "Prebiotic" means food substances intended to promote the growth of probiotic bacteria in the intestines. Prebiotics are defined for example by Glenn R.
Gibson and Marcel B. Roberfroid, Dietary Modulation of the Human Colonic Microbiota [Food Modulation of the Human Colonic Microbiota: Introducing the Concept of Prebiotics], J. Nutr. 1995 125: 1401-1412. The prebiotic can be selected from the group consisting of oligosaccharides and optionally, contains fructose, galactose, mannose, soy and / or inulin; and / or food fibers.
The composition of the present invention may also contain protective hydrocolloids (such as gums, proteins, modified starches), binders, film-forming agents, encapsulating agents / materials, wall / layer materials., matrix compounds, coatings, emulsifiers, surface active agents, solubilizing agents, (oils, fats, waxes, lecithins, etc.), adsorbents, vehicles, filling agents, co-compounds, dispersing agents, wetting agents, aid agents for processing (solvents), fluidizing agents, taste masking agents, agents for the increase of specific weight, gelling agents, gel forming agents, antioxidants and antimicrobials. The composition may further include pharmaceutically conventional additives and adjuvants, excipients and diluents, including, but not limited to, water, gelatin of any origin, vegetable gums, lignin sulfonate, talc, sugars, starch, gum arabic, vegetable oils, polyalkylene glycols, flavoring agents, preservatives, stabilizers, emulsifying agents, buffer solutions, lubricants, colorants, wetting agents, fillers and the like. In all cases, said additional components will be selected considering their relevance to the desired recipient.
The composition of the present invention can contain any number of Bifidobacteria according to the present invention. In principle, only one
Bifidobacteria that reaches the intestine and that can generate a colony is enough to generate a beneficial effect on the host.
It is clear to those skilled in the art that an ideal dose will depend on the individual to be treated, for example, their health condition, gender, age or weight. By
Consequently, the dose to be used will ideally vary but can be easily determined by those skilled in the art.
However, it is generally preferred if the composition of the present invention comprises between 03 and 1010 cfu and / or between 103 and 1010 cells of Bifidobacterium longum NCC2916 per daily dose. It may further comprise between 103 and 1012 cfu and / or between 103 and 1012 cells of Bifidobacterium longum NCC2916 per g of the dry weight of the composition.
The present invention further relates to the use of a Bifidobacterium longum NCC2916 to increase the number of live probiotic bacteria, for example Bifidobacterium longum, in a product. Similarly, the present invention further relates to the use of the composition of the present invention to increase the number of live probiotic bacteria, for example Bifidobacterium longum, in a product.
The uses of the present invention also serve to increase the number of probiotic bacteria, in particular of Bifidobacteria, in a product after exposure to stress for probiotic bacteria.
The stress may be an exposure to oxidative species, for example, in a concentration of 200 ppm, for example, for 30 seconds to 2 hours.
Oxidative species are not particularly limited. Very common reactive oxygen species include, for example · 02 ', the superoxide anion; H202,
hydrogen peroxide; · ??, the hydroxyl radical; ROOH, organic hydroperoxide; RO, alkoxy radicals; ROO », peroxy radicals; HOCI, hypochlorous acid; OONO-, peroxynitrite; I ??·. Preferably, the reactive oxygen species are selected from the group consisting of H202, · 02 ', · ?? , and combinations thereof.
Those skilled in the art will understand that they can freely combine all of the features of the present invention described herein without departing from the scope of the invention as disclosed. In particular, the features described for the use of the present invention can be applied to the composition of the present invention and vice versa.
The additional features and advantages of the present invention are apparent from the following Examples and Figures. or
Figure 1 shows viable cell counts of cultures of 16 h overnight at 4 h in 200 ppm H202. NCC2916 (white bars) and wild type NCC2705 (black bars). The p values refer to a test t with the wild type Example 1:
Identification and isolation of NCC2916
Approximately 1011 cfu of NCC2705 were resuspended in 10 ml of 40 ppm of H202 for 80 minutes. A volume of 1 ml of cell suspension was subsequently plated on MRS agar. A visible colony within 48 h was subcultured 2 times overnight (16 h) in MRS broth and frozen at -80 ° C for further analysis.
To compare the resistance to H202 of the wild-type cells and the new variant NCC2916, frozen samples of cells (overnight cultures, 16 h) were thawed from wild-type and variant cultures with the same densities and aliquots of 1 were centrifuged. mi (6? 00 g, 2 min at room temperature). The pellet was resuspended in a solution in 10 ml of 200 ppm H202. After 4 h, the cell suspension was diluted in a buffer solution and plated. Colony counts were measured after 48 h. The test was performed in triplicate (Fig 1). The tolerance of NCC2916 to H202 remained stable for at least 70 generations.
Claims (10)
1 . Bifidobacterium longum NCC2916.
2. A composition characterized in that it comprises a Bifidobacteria according to Claim 1.
3. The composition according to Claim 2, characterized in that the composition is a food product, a food product for animals or a pharmaceutical composition.
4. The composition according to one of Claims 2-3, characterized in that the composition also contains at least one prebiotic.
5. The composition according to claim 4, characterized in that the prebiotic is selected from the group consisting of oligosaccharides and optionally, contains fructose, galactose, mannose, soy and / or inulin; and / or food fibers.
6. The composition according to one of Claims 1 - 5, characterized in that it comprises between 103 and 1010 cfu and / or between 103 and 1010 cells of Bifidobacterium NCC2916 per daily dose.
7. The composition according to one of Claims 1 - 5, characterized in that it comprises between 103 and 1012 cfu and / or between 103 and 1012 of Bifidobacterium NCC2916 cells per g of the dry weight of the food composition.
8. The use of a Bifidobacterium according to Claim 1 or of a composition according to Claims 1 - 7, characterized in that it serves to increase the number of live probiotic bacteria in a product.
9. The use according to claim 8, characterized in that it serves to increase the number of probiotic bacteria in a product after exposure to stress for probiotic bacteria.
10. The use according to Claim 9, characterized in that the stress is an exposure to oxidative species, in a concentration of 200 ppm, by 30 seconds to 2 hours. eleven . The use according to Claim 10, characterized in that the oxidative species is selected from the group consisting of H202, · 02 ', OH', and combinations thereof.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP09164541A EP2270134A1 (en) | 2009-07-03 | 2009-07-03 | Oxidative stress resistant Bifidobacteria |
| PCT/EP2010/059530 WO2011000965A1 (en) | 2009-07-03 | 2010-07-05 | Oxidative stress resistant bifidobacteria |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| MX2012000259A true MX2012000259A (en) | 2012-01-25 |
Family
ID=41258160
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| MX2012000259A MX2012000259A (en) | 2009-07-03 | 2010-07-05 | Oxidative stress resistant bifidobacteria. |
Country Status (10)
| Country | Link |
|---|---|
| US (1) | US20120177607A1 (en) |
| EP (2) | EP2270134A1 (en) |
| JP (1) | JP2012531192A (en) |
| CN (1) | CN102741392A (en) |
| AU (1) | AU2010267924A1 (en) |
| BR (1) | BR112012000102A2 (en) |
| CL (1) | CL2012000012A1 (en) |
| MX (1) | MX2012000259A (en) |
| SG (1) | SG176953A1 (en) |
| WO (1) | WO2011000965A1 (en) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AU2016315268B2 (en) * | 2015-08-31 | 2022-12-22 | Société des Produits Nestlé S.A. | Methods and compositions using Bifidobacterium longum to modulate emotional reactivity and treat or prevent sub-clinical mood disturbances |
Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS53121949A (en) * | 1977-03-31 | 1978-10-24 | Yakult Honsha Kk | Production of drink and food containing bifidobacterium cells |
| JPS61185182A (en) * | 1985-02-14 | 1986-08-18 | Meiji Milk Prod Co Ltd | Mutant strain of bifidus and cultivation composition thereof |
| EP1227152A1 (en) * | 2001-01-30 | 2002-07-31 | Société des Produits Nestlé S.A. | Bacterial strain and genome of bifidobacterium |
| ITBO20020564A1 (en) * | 2002-09-06 | 2004-03-07 | Alfa Wassermann Spa | BIFIDOBACTERIA AND PREPARATIONS THAT CONTAIN THEM. |
| CN101314763B (en) * | 2007-06-01 | 2011-06-08 | 统一企业(中国)投资有限公司 | Short bifidobacteria with functions of anti-gastrointestinal tract pathogen, oxidation resistance and blood pressure reduction and its uses |
-
2009
- 2009-07-03 EP EP09164541A patent/EP2270134A1/en not_active Withdrawn
-
2010
- 2010-07-05 EP EP10730770A patent/EP2449088A1/en not_active Withdrawn
- 2010-07-05 MX MX2012000259A patent/MX2012000259A/en not_active Application Discontinuation
- 2010-07-05 JP JP2012516798A patent/JP2012531192A/en not_active Withdrawn
- 2010-07-05 US US13/382,096 patent/US20120177607A1/en not_active Abandoned
- 2010-07-05 CN CN2010800300612A patent/CN102741392A/en active Pending
- 2010-07-05 AU AU2010267924A patent/AU2010267924A1/en not_active Abandoned
- 2010-07-05 WO PCT/EP2010/059530 patent/WO2011000965A1/en active Application Filing
- 2010-07-05 BR BR112012000102A patent/BR112012000102A2/en not_active IP Right Cessation
- 2010-07-05 SG SG2011095064A patent/SG176953A1/en unknown
-
2012
- 2012-01-03 CL CL2012000012A patent/CL2012000012A1/en unknown
Also Published As
| Publication number | Publication date |
|---|---|
| JP2012531192A (en) | 2012-12-10 |
| EP2270134A1 (en) | 2011-01-05 |
| AU2010267924A1 (en) | 2012-02-02 |
| EP2449088A1 (en) | 2012-05-09 |
| CL2012000012A1 (en) | 2012-10-12 |
| WO2011000965A1 (en) | 2011-01-06 |
| SG176953A1 (en) | 2012-02-28 |
| CN102741392A (en) | 2012-10-17 |
| US20120177607A1 (en) | 2012-07-12 |
| BR112012000102A2 (en) | 2016-08-09 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Beheshtipour et al. | Supplementation of Spirulina platensis and Chlorella vulgaris algae into probiotic fermented milks | |
| Chou et al. | The applications of polysaccharides from various mushroom wastes as prebiotics in different systems | |
| US9101651B2 (en) | Lactobacillus rhamnosus and weight control | |
| CN101262779B (en) | Probiotic health or fitness promoting foodstuff, feed, and/or drinking water additive and use thereof | |
| US11026983B2 (en) | Bifidobacterium breve CBT BR3 strain for promotion of growth and nutraceutical composition for promotion of growth containing the same | |
| WO2010091992A1 (en) | Lactobacillus rhamnosus ncc4007, a probiotic mixture and weight control | |
| EP2323504A1 (en) | Temperature-induced delivery of nutrients by micro-organisms in the gastrointestinal tract | |
| JP5499231B2 (en) | Animal feed composition containing Lactobacillus plantarum, combined animal feed containing the composition, and method for maintaining or growing the Lactobacillus plantarum in the animal intestine | |
| Kumar et al. | Bifidobacteria for life betterment | |
| ES2965365T3 (en) | Bacterial strain and its use | |
| US20240075080A1 (en) | Use of lactic acid bacteria to inhibit methanogen growth or reduce methane emissions | |
| MX2012000259A (en) | Oxidative stress resistant bifidobacteria. | |
| Darilmaz | Relationship between gastrointestinal tolerance and exopolysaccharide production of propionibacteria strains under different pH and bile conditions | |
| Boonmee et al. | Effects of Musa (ABB group) prebiotic and Bacillus subtilis S11 probiotic on growth and disease resistance of cultivated Pacific white shrimp (Litopenaeus vannamei). | |
| Arihara | Probiotics | |
| González-Ávila et al. | Evaluation of prebiotic potential of agave fructans from different regions of Colima and Zacatecas, Mexico. | |
| Kannika et al. | Enhanced production rate of probiotic Enterococcus faecium A028 by repeated batch solid-state fermentation and its survival under hot air drying with cell-protective agents | |
| Guo et al. | Comparative evaluation of three Lactobacilli with strain-specific activities for rats when supplied in drinking water | |
| Visi et al. | How to maintain the effective levels of probiotics throughout the shelf life in yoghurt: A review | |
| US20210030819A1 (en) | Edible plant parts enriched with probiotic bacteria | |
| Akweya et al. | Isolation, biochemical characterization and safety screening of potential probiotic lactic acid bacteria from spontaneously fermented cereal products from Western Kenya | |
| Julean et al. | Lyophilisation of Probiotic Bacteria for Inclusion in Poultry Feed | |
| CZ16153U1 (en) | Probiotic wide-spectrum nutrition composition in powder, liquid, pasty or gaseous form |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| FA | Abandonment or withdrawal |