MX172696B - NEW ANTIGENS AND METHOD FOR ITS PRODUCTION - Google Patents

NEW ANTIGENS AND METHOD FOR ITS PRODUCTION

Info

Publication number
MX172696B
MX172696B MX2196890A MX2196890A MX172696B MX 172696 B MX172696 B MX 172696B MX 2196890 A MX2196890 A MX 2196890A MX 2196890 A MX2196890 A MX 2196890A MX 172696 B MX172696 B MX 172696B
Authority
MX
Mexico
Prior art keywords
protein
sequence
modified
necessary
production
Prior art date
Application number
MX2196890A
Other languages
Spanish (es)
Inventor
Apolonia Rutgers
Pierre Voet
Eric Jacobs
Cornelis P Hollenberg
Zbigniew A Janowicz
Armin J Merckelbach
Martin Comberbach
Nigel Harford
Teresa Cabezon
Original Assignee
Smithkline Beecham Biolog
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Smithkline Beecham Biolog filed Critical Smithkline Beecham Biolog
Priority to MX2196890A priority Critical patent/MX172696B/en
Publication of MX172696B publication Critical patent/MX172696B/en

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Abstract

La presente invención se refiere a un metodo para producir una proteína de superficie grande, modificada del virus de la hepatitis B, que comprende una secuencia de aminoácidos que codifican para la proteína L, caracterizada por una modificación en cuando menos una de las siguientes secuencias: una secuencia sensible a la digestión con proteasa, una secuencia necesaria para la miristilación, una secuencia necesaria para la glicosilación unida a O, y una secuencia necesaria para el enlace de la albúmina de suero humano, el metodo está caracterizado porque comprende cultivar una celula huesped que comprende una secuencia de codificación de ADN para la expresión de la proteína L modificada en medios de cultivo apropiados y aislamiento de la proteína del lisado celular o el extracto del cultivo.The present invention relates to a method of producing a large surface protein, modified from the hepatitis B virus, comprising an amino acid sequence encoding the L protein, characterized by a modification in at least one of the following sequences: a sequence sensitive to protease digestion, a sequence necessary for myristylation, a sequence necessary for O-linked glycosylation, and a sequence necessary for binding of human serum albumin, the method is characterized in that it involves culturing a host cell comprising a DNA coding sequence for expression of the modified L protein in appropriate culture media and isolation of the protein from the cell lysate or the culture extract.

MX2196890A 1990-08-14 1990-08-14 NEW ANTIGENS AND METHOD FOR ITS PRODUCTION MX172696B (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
MX2196890A MX172696B (en) 1990-08-14 1990-08-14 NEW ANTIGENS AND METHOD FOR ITS PRODUCTION

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
MX2196890A MX172696B (en) 1990-08-14 1990-08-14 NEW ANTIGENS AND METHOD FOR ITS PRODUCTION

Publications (1)

Publication Number Publication Date
MX172696B true MX172696B (en) 1994-01-07

Family

ID=19742585

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2196890A MX172696B (en) 1990-08-14 1990-08-14 NEW ANTIGENS AND METHOD FOR ITS PRODUCTION

Country Status (1)

Country Link
MX (1) MX172696B (en)

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