MD845G2 - Method for determination of lipolytic ferments in the microbiological objects - Google Patents
Method for determination of lipolytic ferments in the microbiological objectsInfo
- Publication number
- MD845G2 MD845G2 MD96-0381A MD960381A MD845G2 MD 845 G2 MD845 G2 MD 845G2 MD 960381 A MD960381 A MD 960381A MD 845 G2 MD845 G2 MD 845G2
- Authority
- MD
- Moldova
- Prior art keywords
- lipolytic
- ferments
- activity
- solution
- analysed
- Prior art date
Links
Landscapes
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
The invention relates to the microbiological engineering and may be used in the medicine, food, textile and tannary industries at the preparation of washing agents for the municipal economy and for sewage treatment.The summary of the invention consists in the preparation of the reaction mixture from the analysed solution, tributyrine and veronal buffer, pH 8,4, in the incubation of the prepared mixture at a temperature of no more than 47°C with further addition of 0,5 M KF solution as inhibitor, in the determination of the free fatty acids by the spectrophotometric method at the wave length of 540 nm. The calculation of the ferments lipolytic activity is made according the formula:wherein:LFŔ - lipolytic ferments activity;ODc. - optical density of control;ODan. - analysed solution optical density;t - incubation time, h;c - analysed solution concentration,mg/ml;v - analysed solution volume, ml;k - recalculation coefficient, equal to 10416 nM/s x g.The technical result of the invention consists in increasing the activity manifestation of the lipolytic enzymes.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| MD96-0381A MD845G2 (en) | 1996-12-10 | 1996-12-10 | Method for determination of lipolytic ferments in the microbiological objects |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| MD96-0381A MD845G2 (en) | 1996-12-10 | 1996-12-10 | Method for determination of lipolytic ferments in the microbiological objects |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| MD845F1 MD845F1 (en) | 1997-09-30 |
| MD845G2 true MD845G2 (en) | 1998-06-30 |
Family
ID=19738932
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| MD96-0381A MD845G2 (en) | 1996-12-10 | 1996-12-10 | Method for determination of lipolytic ferments in the microbiological objects |
Country Status (1)
| Country | Link |
|---|---|
| MD (1) | MD845G2 (en) |
-
1996
- 1996-12-10 MD MD96-0381A patent/MD845G2/en active IP Right Grant
Non-Patent Citations (2)
| Title |
|---|
| Микробиологический журнал, т. 50, №2, 1988 г., М., В.В. Смирнов, Н.Т. Слабоспицкая, С.С. Крымовская и др."Липолитическая активность спорообразующих аэробных бактерий, выделенных из различных источников", с. 6-7. * |
| Т.И. Билай, "Термостабильные ферменты грибов", 1979 г., "Наукова Думка", Киев, с. 122. * |
Also Published As
| Publication number | Publication date |
|---|---|
| MD845F1 (en) | 1997-09-30 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| FG3A | Granted patent for invention | ||
| IF99 | Valid patent on 19990615 |
Free format text: EXPIRES: 20161210 |