MD558G2 - Assay of protease microquantrties activity - Google Patents

Assay of protease microquantrties activity

Info

Publication number
MD558G2
MD558G2 MD95-0314A MD950314A MD558G2 MD 558 G2 MD558 G2 MD 558G2 MD 950314 A MD950314 A MD 950314A MD 558 G2 MD558 G2 MD 558G2
Authority
MD
Moldova
Prior art keywords
activity
assayed
assay
plate
gelatine
Prior art date
Application number
MD95-0314A
Other languages
Romanian (ro)
Russian (ru)
Other versions
MD558F1 (en
Inventor
Elimira Perepelita
Alexandru Cosiacov
Original Assignee
Национальный Институт Винограда И Вина
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Национальный Институт Винограда И Вина filed Critical Национальный Институт Винограда И Вина
Priority to MD95-0314A priority Critical patent/MD558G2/en
Publication of MD558F1 publication Critical patent/MD558F1/en
Publication of MD558G2 publication Critical patent/MD558G2/en

Links

Landscapes

  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Peptides Or Proteins (AREA)

Abstract

The invention relates to the biochimistry and may be used in biotechnology, plat physiology and biochemistry, in medicine and agriculture.The technical result consist in increasing sensibility, objectivity and universality of assay method.The method comprises the preparation of a plate coated with gelatine, dropping of assayed substame onto plate and kuping in thermostate. Then gelatien lager is irradiated by light and illumination below plate is measund throynh the obtained spot as result of gelatine hydrolysis by both assayed proteaze and commercial protozoa as reference. The protozoa activity is estimated according to proposed formula.
MD95-0314A 1995-06-14 1995-06-14 Assay of protease microquantrties activity MD558G2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
MD95-0314A MD558G2 (en) 1995-06-14 1995-06-14 Assay of protease microquantrties activity

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
MD95-0314A MD558G2 (en) 1995-06-14 1995-06-14 Assay of protease microquantrties activity

Publications (2)

Publication Number Publication Date
MD558F1 MD558F1 (en) 1996-05-31
MD558G2 true MD558G2 (en) 1996-12-31

Family

ID=19738716

Family Applications (1)

Application Number Title Priority Date Filing Date
MD95-0314A MD558G2 (en) 1995-06-14 1995-06-14 Assay of protease microquantrties activity

Country Status (1)

Country Link
MD (1) MD558G2 (en)

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
Picford G.E. and Dorris F., 1934, Micromethods for the detection of proteases and amylases . Science, 80, ń. 317-319. *
Васильева Р.И., Евтихов Н.Н., Болград Г.В.. Соотношение величин активности протеиназы, полученных двумя способами измерения. Микробиологическая промышленность. 1976, n. 4, с. 23-26. *
Плешков Б.П.. Практикум по биохимии растений. "Колос", 1968. *

Also Published As

Publication number Publication date
MD558F1 (en) 1996-05-31

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Legal Events

Date Code Title Description
FG3A Granted patent for invention
IF99 Valid patent on 19990615

Free format text: EXPIRES: 20150614