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Application filed by Институт Микробиологии И Биотехнологии, Институт Химии, МокиfiledCriticalИнститут Микробиологии И Биотехнологии
Priority to MDS20210053ApriorityCriticalpatent/MD1624Z8/en
Publication of MD1624YpublicationCriticalpatent/MD1624Y/en
Publication of MD1624ZpublicationCriticalpatent/MD1624Z/en
Publication of MD1624Z8publicationCriticalpatent/MD1624Z8/en
Micro-Organisms Or Cultivation Processes Thereof
(AREA)
Abstract
The invention relates to biotechnology, namely to a process for submerged cultivation of Rhizopus arrhizus CNMN FD 03 fungus strain, producer of lipases and can be used in the microbiological industry for producing lipolytic enzymes with wide application in the food industry, production and processing of fats and vegetable oils, in medicine in as a therapeutic and diagnostic agent.The process, according to the invention, provides for the production of a spore suspension of a strain grown for 30 days on a malt-agar medium, inoculation of the suspension in an amount of 5vol.% into a nutrient aqueous medium containing, g/L: soy flour - 35.0, (NH4)2SO4 - 1.0, KH2PO4 - 5.0, with simultaneous introduction of 0.005 g/L of [SrL3][Co(NCS)4], where L - dimethylpyridine-2,6-dicarboxylate, and cultivation with constant stirring at 180-200 rpm for 24 hours, at a temperature of 28-30°C.The result of the invention consists in increasing the biosynthesis of lipolytic enzymes and reducing the duration of cultivation of the strain by 24 hours.
MDS20210053A2021-06-232021-06-23Process for submerged cultivation of Rhizopus arrhizus CNMN FD 03 fungus strain, producer of lipases
MD1624Z8
(en)
Mass production of exo-polysaccharide from submerged cultivation of Ganoderma lucidum by agitation and aeration effect under bi-staged pH controlling system of jar fermenter