MD1279Y - Method for estimating the toxicity of chemicals - Google Patents

Method for estimating the toxicity of chemicals

Info

Publication number
MD1279Y
MD1279Y MDS20170067A MDS20170067A MD1279Y MD 1279 Y MD1279 Y MD 1279Y MD S20170067 A MDS20170067 A MD S20170067A MD S20170067 A MDS20170067 A MD S20170067A MD 1279 Y MD1279 Y MD 1279Y
Authority
MD
Moldova
Prior art keywords
addition
toxicity
chemicals
samples
estimating
Prior art date
Application number
MDS20170067A
Other languages
Romanian (ro)
Russian (ru)
Inventor
Ион ТОДЕРАШ
Аурелиан ГУЛЯ
Валентин ГУДУМАК
Елена РОШКОВ
Ольга ГАРБУЗ
Original Assignee
Институт Зоологии Академии Наук Молдовы
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Институт Зоологии Академии Наук Молдовы filed Critical Институт Зоологии Академии Наук Молдовы
Priority to MDS20170067A priority Critical patent/MD1279Z/en
Publication of MD1279Y publication Critical patent/MD1279Y/en
Publication of MD1279Z publication Critical patent/MD1279Z/en

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  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

The invention relates to methods for estimating the toxicity of chemicals.The method, according to the invention, comprises the preparation of the Paramecium caudatum culture, addition to the test samples of the tested chemicals in various concentrations, incubation of the test and control samples, addition of the dye 3-amino-7-dimethylamino-2-methylphenazine hydrochloride, incubation followed by addition of the formalin solution, centrifugation of samples, removal of supernatant, addition of sodium hydroxide solution, determination of absorption with a spectrophotometer, afterwards is calculated the percentage of live paramecia and is determined the lethal concentration (LC50), at the same time the lower the LC50 concentration value, the higher the toxicity of the tested chemical.
MDS20170067A 2017-05-23 2017-05-23 Method for estimating the toxicity of chemicals MD1279Z (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
MDS20170067A MD1279Z (en) 2017-05-23 2017-05-23 Method for estimating the toxicity of chemicals

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
MDS20170067A MD1279Z (en) 2017-05-23 2017-05-23 Method for estimating the toxicity of chemicals

Publications (2)

Publication Number Publication Date
MD1279Y true MD1279Y (en) 2018-08-31
MD1279Z MD1279Z (en) 2019-03-31

Family

ID=63435106

Family Applications (1)

Application Number Title Priority Date Filing Date
MDS20170067A MD1279Z (en) 2017-05-23 2017-05-23 Method for estimating the toxicity of chemicals

Country Status (1)

Country Link
MD (1) MD1279Z (en)

Also Published As

Publication number Publication date
MD1279Z (en) 2019-03-31

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Legal Events

Date Code Title Description
FG9Y Short term patent issued
KA4Y Short-term patent lapsed due to non-payment of fees (with right of restoration)