LV12919B - Biogenic veterinary preparation produced from placenta - Google Patents

Abstract

Invention relates to veterinary preparations for stimulating non-specific immunity in animals. The invention describes a method for producing the preparation from dog placenta. The method provides for cutting into small pieces, washing in distilled water and physiological solution, homogenising and incubating at +40C dog placenta. Further steps of the method are: separation of liquid phase, e.g., by centrifugation, incubation of supernatant at - 200C temperature, filtering and freeze drying. The preparation obtained stimulates activity of monocytes and phagocytes. It exhibits antitumor activity.

Description

EN 12919 CIIOCOB ΠΟΠΥΗΕΗΗΑ ΒΗΟΓΕΗΗΟΓΟ ΠΡΕΠΑΡΑΤΑ Η3 nJIAIļEHTbl H3o6peTeHne othochtcs k o6; iacTH BerepHHapHH η φ3ρΜ3κοτερ3ΠΗΗ h Kacaerca BonpocoB co3flaHHa BeTepHHapHHx 6iīcwionraecKH aKTHBHHx npenapaTOB, CTHMy / uipy-ίοπικχ Hecnemi4) iraecKHe MexaHH3MH ημμ > ήηογο OTBera. B HMMyHHHH OTBeT aKTHBHO BOBJICKBIOTCH ΚΛβΤΚΗ KpOBH - MOHOHyKJieapHWe h nojiHHymieapHMe ΗεΗτροφ & τΗ. CHaaa ^ a BmnoaaiOTca ΗβοπβΐίΐΐφΗΗβοκΗβ, a 3aTeM οπειικφίΡίβοκΗε MexaHH3Mu peaKimH πρπ ΗΗφεκιΐΗΗ, ββκρηηβρηη h Bocnajnrrejn > HOM npopecce. Oshhm H3 noKa3aTejieii, xapaKTepH3yjomnx h peryjrapyK) mHX CHCTeMy ΗβΟΠβΙίΗφίΠΟΟΚΟΗ ρβ3Η0ΤβΗΤΗ00ΤΗ OpraHH3Ma 9BJIS6TCa φΒΓ0ΐρΠΌ3. ≪ X > aT0IļHT03 - 3T0 npopecc ποινιοιρεΗΜ mierKOH xpynHHx AACR, βη ^ ημηχ b ΜΗκροοκοπ (ποΒρεχΛεΗ-HCE a missing ιυιετοκ, MHxpoopraHH3Mu, Kpynm > ie BHpycH h FLP.) · Npouecc φβΓοιρϊτοββ μοχηο Pa3 # ejraTB Ha flBe φ83Η b nepByio φa3y aacmpH CBa3biBaiOTca Ha noBepxHocxH MeM6paHH MOHOHyKJieapHHx κλογοκ Φβγοιιητοβ. Bo BTOpyio φa3y aacTHiiH norjioīpaiOTca kjičtkoh h pa3pymaK > Tca. AkthbhoctL · mieroK MOHOHyxjieap-Η0-φθΓ0ΙΪΗΤ3ρΗ0Η CHCTOMH HMO H8n0Cp8flCTB8HH08 OTHO HHHKKHaiHH HMMyHHoro OTBera: ohh 3axBaTHBaiOT η ΜΟΑΗφπιρη ^ ιοτ aHTHreH, 3aTeM npepocraejiaiOT Difference / ιημΦοοητβμ. MoHOHyKJiepmae Φβγορητη yaacTByioT Taxxe b CHHTe3e 6hojio-maecKH βκίήβηηχ BemecTB, φopMHpya ημμυηηηη οτΒετ, b aacrHOcra c8KpempyiOT HHTepieIiHH-1 (ΒΚΤΗΒΒΤΟΡ Τ-ΛΗΜφορΗΤΟΒ). yMem > meHHe 4 > arOIīHTapHOŪ βκτηβηοοίή ΗβΗτροφιίΛΟΒ Βε / ιετ κ xpoHH3aiļHH βοογιβλητολβηογο npoipcca, κ / from mopacamno ayTOHMMyHHoro OTBera, nocxojn > xy npn 3tom Hapymaerca φyκ ^ Ha pa3pymeHHa h BHBefleHHS naTOreHHHX aHTHTeHOB H HMMyHHUX KOMIUieKCOB H3 0praHH3Ma. H3yaeHHe noKa3aTejiež φ3Γ0ρΗΤ033 HMeer 3Haaeirae npn aHajm3e h ΑΜΓΗοετΗκε HMMjraoae-Φηιιητηηχ cocroaHHH: / ρικτβΛΒΗΟ HeaascHBaioinHe ΐΉθΗΗθ-BOcnajiHTejiBHHe npoupccH, pas / onmue oaioacHeHHa, noaaneHHe HOBOo6pa30BaHHH, ΒροχρεΗΗΗε ΑεφεκτΗ φβτο-PHTapHOH cHcreMU, xpoHiraecKHe BOcnajraTejn > Hue 3a6oji8BaHna, jieaeHHe HHTOCTaTH-xaMH h HMMyHo, qeiipeccaHTaMH h ap.

AkthbhoctL · npopecca ^ ftrommm xapaxTepH3yK > T b ochobhom 2 noxa3aTejia: 1) (Pazos, umapHbiū noKū3ameAb - npopeHTHoe coa; epxaHHe ΗεκτροφΗΛΟΒ, yaacTBy-R) nļnx b φ3Γοιριτο3ε, 2) (frazoīņimapHoe HUCA - cpepHee kojihhcctbo ΜΗκροβοΒ, ΠΟΓΛΟίρεΗΗΗΧ OflHHM ΗεΗΤροφΗΛΟΜ KpOBH [ Ί 2]. KNA NPE ^ ^ ynpexc eHHa bo3hhkhob8hhh ΤΗχε / ΐΒΐχ ocjioxh8hhh, B03HHKaioipHX NPH xpOHH3aimH 3a6o ^ eBaHHH, co3flaioTca dnoreirane crHMy / iaTOpbi - ΛεκβρετΒβΗΗΒίε npenapaTH, Cn0C06CTByi0mHe CTHMyjiaiļHH φβΓΟΡΗΤΒρΗΟΗ aKTHBHOCTH ΗεζτροφΗΛΟΒ.

Oco6oe 3Ha * ieHHe HMeeT nPHMeHeHHe 6ηοι «ηηηχ CTHMyyesToPOB npH jHiMeHaMe ^ eCTBeHHtDC HOBO6pa30BaHHH XHMHOTepaneBTHaeCKHMH CpeflCTBaMH. ΠρΟΤΗΒΟ-onyxojieBoe neHcrBHe ποαιβΛΗπχ ocHOBaHO Ha ηχ οποοο6ηοογη pearapoBaTfa c nempaMK 6ejiKoeux MOJieKyji, Hapymaa chht63, Π, ΗΚ, b pe3yjibraTe 6jiOKHpyeTCa ΜΗτοτΗΗβακοβ nejiemie κπετοκ rHnepruia3HpOBaHHbīx (onyxojieBHx) τκβη6η η λημΦοηαηοη τκβηη. BMecre c τβΜ ohh B3aHMoneHCTByiOT c ^ HymieonpOTen Amii κπβτοηΗΗχ anep κροΒβτΒορΗΗΧ kjictok, BCJieflCTBHe aero npoaBjiaerca hx TOKCHaecKoe neācTBHe, jnrHeraeTca nponecc κροΒβτΒορβΗΜ, yMeHBmaerca kojihhcctbo ΗβήτροφιυιοΒ, yraeTa-lOTCa HX XCH3HefleaTeJībHOCTb Η φ3Γ0ηΗΤ3ρΗ33 aKTHBHOCTb. H3BecreH cnoco6 JieaeHHa paxa mojiotooh xejie3hi y co6ax npenapaTOM BHTypHA-B. IIpenapaT co ^ epaoiT TOKCEraecKoe cpe ^ cTBO pTyra flHXJiopHn [3j. Jjja yMeHbmeHHa tokchhhocth npenapaT coefficient ^ HHaioT c opraHHaecKHMH jraraHflaMH, Hcnojn > 3ya 6ejioe cyxoe bhho (100 mji) πρκ npneMe npenapaTa. H3BecTHHH npoTHBOonyxojieBHH npenapT nonaBJiaeT pocr onyxojiH. OflHaKO technology 'o6jianaeT TOKcnaecKHM ^ h encTBneM He cnoco6eH crHMyjiHpoBaTb yBe; raaemie KOjmaecTBa ΗβΗΤρΟφίϋΙΟΒ, He Cn0C06eH CTHMyjIHpOBaTb φηΓΟΙΗΠΏρΗ ^ aKTHBHOCTb ΗβΗΤροφΗΛΟΒ, He oOnaflaeT CTHMy / iHpyionniM fležcreHeM Ha nponecc φ3Γοηητο33. H3BecTeH BerepHHapHUH npenapT cyjn > TenpHM. technology 'πρπΜβΗβιοτ pjia neaeHHH xpoHnaecKHx BOcnajiHTejn > Hhix 3a6onesaHHH 6aKTepHajibH0H 3τηολογηη y CBHHež, πτΗρ, co6ax η κοιπεκ [4] technology' conepaarr cynbφaHHΛaM ^^, τρΗΜβτοπρΗΜ h aHTH6HOTHK H3 rpynnbi TeTpanHKJiHHOB. Πρκ BBeffeHHH npenapaTa BHyTpb oh BcacHBaeTca H3 JKejiynoaHO-KHmeaHoro TpaKTa b KpoBb, npoHHKeT bo ece opraHH h TKaHH χηβοτηηχ, r # e HaxoflHTca 24 aaca. npenapaT no3BOjiaeT ποβη «πί > 3φφβκΐΉΒΗ00ΤΒ jieaeHiia. OflHaxo H3BecTHbm npenapaT They are the CTHMyjiHpyionlHM AežcTBHeM Ha nponecc φ3Γοιηιτθ33, He cnoco6eH craMyjiHpoBaTb φΗτοηκταρΗ ^ aKTHBHOCTb KJieTOK ΚρΟΒΗ. H3BecTeH cnoco6 npHroTOBjieHHH TKaHeBoro nickelTa, npencraBjiaioinero co6oh roMoreHaT TKaHH napeHXHMaT03Horo opraHa - nonacenyflOHHOH xcejie3Hc6hkh H 1 HHHHH (hjih amnca coOaKH). ΤκηΗβΒΟΗ npenapaT ββοαβτ β πολκοχη > το KJieTaaTKy ΗΗΧΗεκ nacTH nepenHež βριοπίΗΟΗ creHKH tsj. If Mecre BBeneHHa co3naeTca MHKpoonar BocnajieHHa, kotophh nocjie co3peBaHHa ΗΗφιυπ > τρητ3 caMonpoH3BOJH &HO; HO BCKpHBaeTca. Co3flaHHe MHKpooaara BocnajieHHa no3BOJiaeT cTHMyjiHpoBaTb Hecnenz-φΗΗβΰΚΗβ MexaHH3MH HMMyHHOIO OTBera, B TOM HHCJie CnOCoScTBOBaTb yBejIHneHHK) KOnHneCTBa ΗβΗΤροφίυΐΟΒ, CTHMyjIHpOBTb HX 4 > aronHTapHyK) aKTHBHOCTb, T.e. CTHMy-jiHpOBaTb nponecc φ3Γοηκτο33. O ^ HaKo C03naHHe MHKaoaarB BocnajieHHa aBjiaerca He6e3pa3JIHHHHM npOneCCOM JLJ1S. OCJia6jieHHbIX 6ojIbHHX ΧΗΒΟΤΗΗΧ, HMeioinnx ocjio * HeHHa, ocodeHHO πρκ ηη / ιηηηη onyxojieBoro nponecca. H3BecTeH BeTepHHapHbiH 6ηογ6ηηηη npenapaT jyia jienemia BHpycHMx 3a6ojie-BaHHH co6aK £ 6 npenapaT nojiyaaii KyjibTHBHpOBaHHeM; HH aacrb φHJIbτpaτa JIHoφHJIH3Hpyκ) τ, pacreopaio b 6yφepHOM pacreope, BHocaT b pacTBop aap030Jia OT, no6aBJOTOT acHpHbie khcjioth, LV 12919 caxapa h cMeraneai pacraopoM aperoHa. nojiyaeHHbiH oca ^ OK pacreopaio b φΗ3ΗΟΛΟΓΗΗ60ΚΟΜ paCTBOpe, CMeiIIHBaiOT CO BTOpOH HaCTbK) φϋΐΙΚ ^ ΤΒ Η ΛΗΟφΗΛΗ-3HpyiOT. rio ^ yqeHHHH npenapaT cooTBSTCTByeT βΗΟΛΟΠΉβακΗ βκτηβηβγμ ΛβκΒροτΒβΗ-hhm cpeflCTBaM. npenapT bboast napeirrepa ^ bHO b A03e 1 nuih e ^. Cnoco6 nojiyneHHfl H3Becraoro npenapaTa aBjiaeTca cjioxhhm. B npopecce co3flaHHa npenappe Tpe6yeTca Harmonized by pa3niniHHx κομποηθητοβ: a3po3o; iH, acnpHbie kucbot, caxapa, aliTOH, opHTHHajibHHH βyφepHHH pacreop. ΠρηΜβΗβΗΗβ aneTOHa pjia. o6e3xnpHBaHHfl κομποηθητοβ b nponecce npHroTOBjieHHfl npenapTa 3HaaHTJibHo CHHxaeT Difference 6HOCTHMynHpyioncae cbohctbe. AMepHKaHCKHe φΒρΜΒΙίεΒΤΗτίβΟΚΗε φκρΜΗ aKTHBHO 3aHHMaiOTCa pa3pa6oTKOH 6HoreHHbix npenapaTOB H3 τκβηη roiaiīeHTH [" 7, 8, 9. B aacraocTH, H3BecTeH cnoco6 npnroTOBJieHHaNHNHHORO nNOtHaT H3 ληοΦηλη3ηροββηηηχ ιυιετοκ njiapembi οβιϊη Γ7 /. technology 'npnroTaBjmBaiOT cjie ^ iomHM o6pa30M: iuianeHTy pa3flejiaioT Ha φρ3ΓΜβΗΤΗ, norpyxaioT b solution was PnHrepa, ητο6η yflajiHTb cjie ^ H κροΒΗ, 3aTeM φρβΓΜβΗΤΗ norpyxaK > T b 5% βοληηζ solution was TpnncHHa, nepeMenmBaioT, φιυικτ-pyiOT, juieTKH OT ^ shadow h npoMHBaiOT b oxjiaxfleHHOM pacreope XeHKca, cMecb u; eHTp? ynipyK) T, no; iyaaK) CycneH3Hio, KOTopyio ληοΦηλη3ηρ > τοτ. BnoreHHBiH npenapaT H3 ληοΦηλη3ηρο33ηηβιχ κτετοκ rniapeHTH Hcnojib3yiOT ara Jienemis a3B η ΟΧΟΓΟΒ. B repMaHHH pa3pa6oTaH 6ηογ6ηηηη npenapaT b φορΜε pacreopa nna ΗΗΐ > εκΐ], ΗΗ Placenta Compositum. npenapaT b aMnyjiax from 2.2 μλ, b ynaxoBKe 100 aMny / i. npenapaT BHnycxaeT φιιρΜΒ HEEL (Germany) flOj. B Ήποηηη C03flaHH npOTHBOBHpycHHe h npoTHonyxojieBbie theyCapcTBeHHHe cpe ^ CTBa H3 KynbTypbi ΚΛβτοκ imapeHTH qe ^ OBexa fll]. B KnTae βκτηβηο pa3pa6aTHBaiOTca 6HoreHeNePPa H3 iuiapeHTH αe α K is is is is is po po po po po po po po po po po po po po po po po po po po po po po po po:::::::::::::::::. B npopecce pa3pa6oTKH npenapaTOB H3 iuiapeHTbi BuaBjieHO, whereby to jļjisl coxpaHeHna 95% 6HCwiorHaecKH βκτηβηηχ BemecTB χε ^ τεΛΒΗΟ b nponecce npiiroTOBjieHHa ocymecmaaTb τολπ > κο CJieflyK > MHE MaHHnyjianHH c iuiapeHTOH: npoMHBaHHe, H3Mejib-aeHHe h cyxoe 3aMopaxHBaHHe (ληοΦηλη33ρηκ)). BnoreHHUH npenapT H3 rnipemic n o sts b BH ^ e μολκογο noponnca p3, 14].

B Pocchh pa3pa6oTaH cnoco6 no ^ yaeHna H3 τκβηη nnaneirru Bemecrea, ycKopionier familyHepapmo TKaHeft npn taeaeHHH paH η οχογοβ £ 15]. Pa3pa6oTaHbi cnoco6 nojiyaeHHa pa3 n n HHX 6noreimbix npenapTOB H3 njianeHTbi fi6, 17, 18], B 1987 r. yTBepx £ eHa «OapMaKonemma craiba 42-42-1491-87 " Ha jieae6Hbm 6κθΓεΗΗΗΗ npenapaT H3 nnaperu aejiOBeica - Θκαηρακτη njiaiļenmu dji * ιΐΜ, βκψιϋ. JIeae6HHH βκοΓεΗΗΗΗ npenapT Suspension Placentae pro injectionibus h cnoco6 difference nojiyaeHna onHcaHu b οφΗηΗ > cnpaBoaHHKe «JleKapcTBeHHHe cpeacrBa» (1985) [19]. npenapaT noJiyaai oiefljraomHM cnocofioM; eHTy aenoeeKa KOHcepBHpyiOT

Ha xojiOffe, H3MeabHaioT ao CocThy B3secH b H3OTOHHHecK0M pacTBope HaTpna is a 117J. BnoreHHHH npenapTBBinGein b BHfle roMoreHaTa b3bcch KpacHO-KopnaHeBoro neeTa (pH 5.8-6.9). CTepHnH3yioT npn + 120 ° C b Tenemie naca. ΠρκΜβΗΑίοτ KaK βκΟΓεΗΗΗΗ CTHMy ^ aTop. Ββοαητ ποα κοχγ of 2 μλ 1 pa3 nepe3 7 AHeS. Kypc - 4 Ηΐπ, εκηΗΗ. OopMa BbinycKa - aMnyjibi no 2 μλ, b ynaKOBKe - 6 yuan. XpaHeHne - b 3anmmeHHOM ot cBere Maecre. , Ηαηηηη cnoco6 npnroTOBjieHHa jiene6Horo 6noreHHoro noppaTa H3 iuianeHTH aBjiaerrcz npomomunoM ηβοβρβτβΗΗΗ. Hcno ^ B30BaHne nponecca crepiflH3aiiHH npn + 120 ° C b Tenemie 1 naca (b προτοτΗπε) 3HanHTenBHO yMeHbmaeT aHTHBHbie CTHMyjrapyionHe CBOHcro npAp [13, 14J. IļejibK) H3o6peTeHHa aBJiaeTca pa3pa6oTKa cnoco6a nojiyneHHs BerepHHapHoro 6noreHHoro technology 'H3 iuianeHTbi, oSjiaAaiomero hkthbhhm CTHMyjmpyiomHM AencTBHeM Ha npopeccH ΗεεπερΗφΗηεοκοΓΟ MexaHH3Ma HMMyHHoro OTBeTa, no3Bona-loniero 3HannTe ^ BHO ποβηοητβ Hecnen ^ HnecKjrco pe3HcreHTHOCTb opraHH3Ma, τβκημ o6pa30M ycKOpHTb HHHimaiļHK) aseKBaraoro nMMyHHoro oTBera, τβΜ Cambes ycKopHTb pa3pymeHne n BHBeAeHHe naToreHHbnc airrareHOB η κοΜίυιεκοοΒ H3 opraHH3Ma 6oAbHoro χηβοτηογο. OAHOBpeMeHHo 6ηογ6ηηηη npenapaT, nojiyneHHHH H3 ryeHTH coiviacHO MeTOflHKe Η3θ6ρετβΗΗΑ, αολχθη o6jiaAaTb npoTHBOonyxojieBHMH CBOHCTBaMH. nocTaBJieHHaa nejib AOCTHraeTca 3a cner τογο, whereby to iuiaiļeHTy co6axH H3MeflbnaK > T, φρΒΓΜεΗΤΗ nnapeHTH cHanajia οτμηββιοτ AHCTH / umpoBaHHon boaoh, 3aTeM npOMHBaiOT φΗΒΗΟΛΟΓΉΗεΟΚΗΜ paCTBOpOM Η B hem rOMOreHH3HpyiOT, TOMOreHaT HHKy6npyiOT npn + 4 ° C, οτρ & ΐίποτ χα ^ κγιο φρΒκιριιο, HanpnMep, ^ eHτpHφyΓHpoBa-HneM, HaflOcaAOHHyio χηακοοπ »HHKy6HypHyNpn -20 ° C, φιυπ > τρ > ποτ η ληοΦηλη-3HpyiOT.

Hobhž BeTepHHapHHH 6ηογ6ηηηη technology ', ποΛγπβΗΗΗπ coraacHO ΜετοΑΗκε Η3θ6ρβτβΗΗΑ, o6jiaaaer cmMyjmpywmHM AencraneM Ha aKTHBHOCTb ιαιετοκ mohohjtk-ΛβΗρΗΟ-φΗΓΟίρίΤΗρΗΟΗ CHCTCMbl, CTHMyjIHpyβΤ HeCneiH «L) HHeCKHe MexaHH3MbI HMMyH-HOH peaKiņiH, cnoco6cTsyeT ηηηιιηβηηη alkyl eKBaraoro HMMyHHoro OTBeTa, πρκ κοτοροΜ HanHHaiOT aKTHBHO pa3pymaTbca η βηβοαητβοη H3 opraHH3Ma 6ολβηογο χηβοτηογο naToreHHH aHTHreHH h KOMruieKCH. OAHOBpeMemio BeTepHHapHHH 6ηογ6ηηηη npenapaT H3 nnapembi o6jajaneT npOTHBOonyxo / ieBbiMH οβοηοτββμη,

Ah3jih3 CTHMy ^ Hpyioniero AežcTBHa npenapaTa If cocrosHHe ΗβοπβρΗφΗηεςκοΗ pe3HCTeHTHOCTH HMMyHHOH CHCTeMbI ηρΟΒΟΑΗΛΗ ΠΟ OlleHKe aKTHBHOCTH φΗΓΟΙ1ΗΤ033 η φ3Γ0ΐ1ΗΤ3ρΗ0Γ0 HHCjia fl, 2]. AHajiH3 npoTHBOonyxojieBoro α6ηοτβηη npenapaTa ocymecTB./i5L/iH no xapaKTepncTHKe KjiHHHHecKOH KapTHHbi 3a6o ieBaHH5i.

MeTOAHKa cnoco6a nojiyHeHHH BeTepHHapHoro SnoreHHoro neppaTa H3 rniapeHTH QieAyK > country. IIpenapaT πρηγοτ3βληβ3Κ) τ H3 TKaHež τροφοβ, Μετβ iuiapeHTH co6axH. NanļļHTβa ieieOJOJβ ηηθββ θρieββejρρie nannannannan nannanr ,βnanβρρβρanannannannannannannannannannannannannannannannan OTo6paHHyio ruiaiieHTy mIjrr b crepHJibHbie MemKH, ynaxoBHBaiOT b CTepHjibHyK > mieHKy h ao TpaHcnopTHpOBKH xpaHJiT b xonOAmibHHKe npn + 4 ° C He 6onee cyTox. ri / ianeHTy ΗεοβχοΑΗΜΟ AoeraBHTb b Tenemie

LV 12919 24 HaCOB C MOM6HTE pOflOB. JljlSl pa60TbI ΗβοβχΟΛΗΜ CTepHJIbHHH φΗ3ΗΟΛΟΓΗΗεθΚΗΗ pacTBop H3 pacneT 4000 mji Ha o6pa6oTKy oahoh iuiaueHTH. Pa6oTa npoBOAHTca b CTepHjebHOM 6oKce. IX / chitin BpyHHyio ocBo6oxflaiOT οτ οβοΛοηβκ η βηαημηχ crycncoB κροΒΗ. npOMHBaioT φΗ3Η0Λ0ΠΜβ0ΚΗΜ pacTBopoM Shiny xjiopHAa, 3Ae-JaiOT Ha ΑΟΛΗ pa3MepOM 4x4 CM. OTMHBaiOT AHCm / UIHPOBaHHOH BOAOH. BODYBOOK 3-KpaTHO φΗ3Η0Λ0ΓΗΗβ0ΚΗΜ paCTBOpOM.TxaHH AOJDKHH 6uTb 6eCKpOBHHMH CBeTAO-po30Boro HBBT. KycoHKH imaueHTH roMoreHH3HpyiOT b cTeMeHom roMoreHH3aTope flO OflHOpOAHOH ΓΟΜΟΓβΗΗΟΗ B3B6CH. ΓΟΜΟΓβΗβΤ HHKy6HpyiOT npH + 4 ° C B ΤβΗβΗΗβ cyTOK. Take & jmiot schakjho φρ3ΚΗΗΐο roMoreHaī ^ 6HτpHφyπīpoBaHHCM πρκ 700 g (jm6o φπΛΒτρΗΗΗβή). HaAOcaAOHHyio χηακοογβ ΗΗκγβπργιοτ πρκ -20 ° C, 3areM φΗ / π »τργκ τ qepe3 MHAmmopoBHe φΗΛΒτρΗ h jm < Ml > HjiH3HpyK) T. 06pa3eu ueneBoro npOAyxTa nepe? B; ia6opTopHio αλα craHAaprH3auHH 6HoreHHoro nano-Tα, no; iHeHHORO H3 miaueHTH co6axH. EHoreHHHH npenapT H3 imaiieHTH co6axH, nojiyqeHHbm corjiacHo MeroAHKe H3o6peTeHHA, 6bUI ΠρΗΜβΗβΗ B BeTepHHapHOH KJIHHHKe KABET (r. Pnra) JLJ1Z. JieqeHHa co6ax c for HHHHMH onyxojiaMH. ΠρΗΜεΗεΗκε 6HoreHHoro nordapaTa noATBepAHJio, hto oho6jiaiAthT ηκτηβηημ cTHMyjiHpyioiuHM achctbhcm Ha nponeccn ΗεεπειίΗφΗΗεεκοΐΌ MexaHH3Ma hmmjthhoio OTBera h npOTHBOonyxojieBHM AencreneM. npenapaT 6bui npHMeHeH αλη AeaeHHa pa3JiHHHHx onyxo / γc y 5 co6ax: onyxonb μολοηηοη xejie3H - y 3 co6ax, onyxojn »nororo - y 1 co6aKH, ocreocapKOMa 6eApeHHOH kocth - y 1 co6aKH.

BceM naval co6axaM 6bum προΒβΑβΗΗ HHbeKUHH OnoreHHoro npenapaTa H3 mianeHTH of 1.5 μα ποακοχηο 1 pa3 b ΗβΑβΑΚ). Kypc - 7 ΗΗΊ > εκφΐή.

Bo Bcex CAyaaax AeueHHa peryuapHO προβοαηαη HccjieAOBaime κροΒΗ c AeŽKOrpaMMOH, aHaAH30M φ3Γ0ΙΪΗΤ033 Η φΒΓΟΑΗΤΒρΗΟΓΟ HHCJ. nocjie npoBeACHHoro xypca HHbeKUHH y Bcex co6ax 6bio OTMeueHO yAynmeHHe C0CT09HH9, noBHmanca aimeTHT, χηβοτηηθ npoaBjisAH βκτηβηοοπ >, HauHHaAH χοαηώ. (ocreocapKOMa), Cypriot h 6eraTb. V co6axH c onyxoAbio uerKoro TeMnepaTypa HOpMajiH30Bajiacb noaie nepBbix Asyx HHbeKUHH, npeKparaAca Kaniejib from HonaM, yAyqmHAca annerar.

Bo Bcex cnynaax AeneHHa ηοβημ 6ηογ6ηηημ npenapTOM 6bia oTMenHa 3HaHHTe / n > Has aKraBamra φβΓορΗΚ ^ h yBejiHHeHHe 4 > arouHapHoro HHCAa nocne HHbeKUHH. Joy AeueHH2 nOKa3aT6Ab φ3Γ0ΗΗΤ033 COCTaBJMA B CpeAHeM 8.7 + 3%, φ3Γ0Ι1ΗΤ3ρΗ0ε HHCAO 6bUIO B ΟρβΑΗβΜ 1.4 + 0.2, ΠΟΟΙβ ΛβΗβΗΗΗ noKa3aTeAb φ3ΓΟΗΗΤ033 - 32 + 4%, φ3Γ0ΗΗΤ3ρΗ0ε HHCAO - 2.5 + 0.4.

KjIHHHHeCKHfi npHMep

BonbHasi co6ukū, 11 ηετ. , ū, HarH03: BbiCTpo pacTymaa onyxonb moaohhoh xene3H. Πρκ ocMOTpe - HOBoo6pa30BaHHe 6x6 cm, AOAbnaToe, 6one3HeHHoe, c oTAeubHHMH onaraMH BOcnajieHHa, Oahh H3 κοτορΗΧ BCKpbuica. Xo3Act co6aKH οτ onepaiura r " _ 'j OTKa3a7iacb. IIpe & noaceHO npOBecTH xypc ηηί > 6κι (ηη 6ηογθηηογο technology 'H3 iuiaiļeHTBi b fl03e 1.5 μλ ποακοχηο 1 Pa3 b Heflejno. ΠροΒε ^ βΗΟ 7 ΗΐΓΒβκΐίΗΗ. Ποα / st noaie ^ HES HHbeKiiHH co6aKa qyecTByeT ce6a xoporao, βκτηβηο HrpaeT. ΓΙρκ ocMOTpe: HOBOo6pa30BaHHe crajio 3HanHTejibHO iuiOTHee h yMeHbnmjiocb b pa3Mepax, onaroB BOcnajieHHH Ηβτ, onyx; ib Ha omym »crajia noxoxceft Ha xpsmeByio cured Fabric. y χηβοτηογο peryjiapHO 6pajra Ha aHajiH3 Kpoeb. ΟπρβΑβΛβΗΗβ craMy; mpy-iomero AežcTBHs technology 'h cocroflHHe HecneiņKļMraecKOH pe3HcreHTHOCTH ημμυη-HOH CHCreMH ΠρΟΒΟΑΗΛΗ ΠΟ ΟΙίβΗΚβ φ3Γ00ΗΤ033 η φΗΓΟΙίΒΓΜρΗΟΓΟ HHC / ia., Π, Ο ΛβΗβΗΗΗ noxa3aTejn > φ3ΓθΐίΗτο33 cocraBjisui 7.2% φβΓΟίηπΉρΗοε ηηοιο - 1.2 nocjie neneHHa cooTBercTBeHHO 219% η 2.1. ĪIpOBeACHHue na6opaTopHbie h KnHHiraecKHe HcaieAOBaHHs noATBepAHjm, whereby to hobhh BerepHHapHMH 6HOreHHHH npena Even nojiyneHHMH H3 iuiaiieHTH co6axH, o6naAaeT CTHMyjiHpyioiniiM AencraneM Ha 3κτηβηοοτβ κηβτοκ ΜΟΗΟΗγισ ^ ρΗΟ-φΒΓΟ-HHTapHOH CHCreMH, aKTHBHpyeT HecnenjKļHraecKHe MexaHH3Mhi ημμυηηοη peaKiņm, cnoco6cTByeT HHHijHaiiHH alkyl eKBaraoro ημμυηηογο οτβθτη, πρκ κοτοροΜ ηκτήβηο pa3pymaiOTca η bhboasitcs η3 opraHH3Ma naToreHHbie aHmreHK η KOMiuieiccbi. OAHOBpeMeHHO hobhh BeTepHHapHHH6HoreHHHH npenapTo nNTTHoBoYoXo-JieBHMH CBOHCTBaMH.

Claims (1)

The method of preparing a biogenic preparation from the placenta by crushing the resulting placenta by washing, homogenizing the sodium chloride in physiological saline, is characterized in that the placenta fragments of the dog are first washed in distilled water, then washed and homogenized, the homogenate incubated at + 4 ° C. remove the liquid fraction, for example by centrifugation, which is incubated at -20 ° C, filtered and lyophilized.