LV11425B - Method of diagnostics of newborn rhesus factor in rh-negative women - Google Patents

Abstract

The blood smear of pregnant women is treated with the human immunoglobulin anti Rh (D), after which the procedure is repeated. Thus, marked preparations after incubation are evaluated by green colouring that shows up on the erythrocyte perimeter. If such colouring appears then a conflict between the mother's and the foetus's rhesus can be anticipated.

Description

The invention relates to medicine, including obstetrics, and directly to peranatology, and relates to maternal-fetal relationships.

• If the mother has rhesus negative blood, the fetus may inherit the father's positive rhesus (D) antigen, which in pregnant women leads to the development of immunoconjunctive pregnancy and the birth of a baby with non-mole disease (JHS). Antiresal immunoglobulin is administered to prevent the development of JHS. Therefore, early identification of the fetal ridge is required. Of particular importance is the precise identification of fetal rhesus in pregnant women with anti - rhesus antibodies in blood serum. It is estimated that 30 7. women with anti-rhesus antibodies (pre-pregnancy or other causes) give birth to rhesus-negative rhesus. In these cases, pregnancy may occur physiologically and may result in parturition (40 weeks). All women with immuno-conflict receive 4-5 non-specific desensitization courses and in-patient infusion therapy. This therapy is not required if the fetus is of rhesus-negative origin. However, in the absence of conclusive diagnostics during pregnancy ~ as a rule immunoconjuncture in pregnant 1 —- q; ·.; ·. d 1 j u m a, v 1 s a m s · i e v 1 e 0 e m is applied to η or knitted t er ap j u uts childbirth is performed by the term 36 .-- 3S. per week to avoid increasing the severity of JHS.

There is a known technique for determining the fetal blood rhesus affinity by piercing (puncturing the fetal bladder film) n by analyzing amniotic fluid during pregnancy 22.36. weekly - add serum-containing NON-BREAKING HYPHEN (8209) containing antibodies to the amniotic fluid, incubate and then add 0.1 ml of rhesus positive red blood cells. The result is evaluated after the agglutination reaction (1).

This method is analogous. The prototype of the invention is not a trust.

According to the authors, the technique is safe in 704 cases. There is a risk of premature termination of mniocentesis. In addition, the entry of fetal blood into the maternal blood stream leads to an increase in immunoconjuncture. It should also be noted that it is desirable to know the detection of a rhesus supplement in the fetus as early as possible during pregnancy. week. Early detection of fetal rhesus supplements is required to cancel the therapy that was □ drawn in the case of an immunocompromised pregnancy.

: Fetal rhesus techniques are performed for detection without the use of amniocentesis, with the aim of increasing pregnancy and preventing premature termination of pregnancy.

The objective of the set is achieved by preparing a difficult-r invention to develop a l1 I tfp e-t = · CllVcfi-iS. ήίΊίϊ * o

j r \ \ --τ - u. . τ r -. . 4. li .i- = i - 'nc · z -r · cr 4 · -> t-, c -r — r w. u ifSub; ι. · -, ι AiUUPG'ijiuULu Ih-u tl ifd t- · LlsMupcz Ratio 23 — t> 0 minutes, rinse, dry, apply loo mkl repetitive antibodies stained with FITC (31 obu line ant i-boom »I i-I i C), incubate at 3 / C for 23-30 minutes, rinse, dry, spread on 50-100 mL / 0.02 of 7. Evans solution, incubate for 5 min. room tempera-

temper restore 50-100 mkl tour, sk al -520 nm.

The smear is examined under a fluorescent microscope at 49020 nm, showing a rhesus positive Rh (D) fetal blood on the appearance of a green staining on the perimeter of the erythrocytes.

The new technique allows accurate detection of fetal rhesus antigen during pregnancy 14-16. week. And can be used if a fluorescent microscope is available.

The proposed method was approbated in the 1st Riga Maternity Hospital for 27 pregnant women with anti-rhesus antibodies in the blood (irounoconflict pregnancy). during pregnancy week. Of the 27 pregnant women examined, 8 were found to have fetal rhesus-negative blood, which was confirmed by delivery in the neonate.

fetal affiliation (by green coloration on the periphery of red blood cells).

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all of these women had children with rhesus positive blood.

demer. Pregnant women

1394 /.--- 70. Blood group P III d. The study diagnosed isosibilization on Eb (D) antigen, antibody titre 1:64. In pregnancy term 14, a new diagnostic method was used for pregnant

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Deficiency of D antigen was diagnosed, i.e. fetus rhesus-negative. Pregnant women were observed until delivery. Pregnancy was terminated by gynecological indications. At week 37 by Caesarean section (Caesarean section in previous pregnancy). The newborn was diagnosed with rhesus-negative blood.

Example 2. Pregnant G., History of Childbirth 943/235. Blood type

d. Diagnosed isosensitization to Rh (D) antigen at a titre of 1:32. At week 16 of the term, treating the pregnant woman's blood smear with anti-rhesus Ph (D) human immunoglobulin at a titre of 1: 512 and applying 100 μl of repeated antibody labeled with FITC, Viewing was performed at 490-520 nm. Conclusion was given regarding rhesus positive (D) fetal blood affiliation. The pregnant woman underwent appropriate treatment. Pregnancy aborted at week 36. A child with rhesus positive blood was born with a diagnosis: Newborn rhesus-bemolytic disease. Child.n performed timely replacement blood transfusion surgery,

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The developed method for the determination of fetal rhesus females in women allows a positive blood fusion to accurately determine fetal rhesus antigens during pregnancy 14-16. week without harming the fetus or mother. The technique can be used in any laboratory where a 1-microscope microscope is available.

Sources of information used to compile the application.

1. Rh Isoimmnization Related to Amniocentesis. J.C.Murray et al.

al., American Journal of Medical Genetics 16: 527-534, No. 4.

Claims (1)

Diagnostic method for rhesus fetal blood females in women with rhesus negative blood is characterized by preparing a pregnant blood smear, applying a 1: 256 to 1: 512 human anti-rhesus (D) immunoglobulin on the smear surface, incubating at 37θ C for 28-30 min. ., rinse, re-dry with 100 µl of antibody, namely labeled FITC (Globulin antihumain FITC), incubate at 37 37C for 28-30 min, rinse, smear, apply 50-100 µl of 0.02% Evans solution, incubate for 5 min. at room temperature, rinse, dry, and observe the smear on a luminescence microscope at 490-520 nm, revealing a green coloration along the erythrocyte perimeter to determine fetal rhesus-positive Rh (D) blood.