LU504482B1 - Method for extracting and separating zingerone from ginger - Google Patents
Method for extracting and separating zingerone from ginger Download PDFInfo
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- LU504482B1 LU504482B1 LU504482A LU504482A LU504482B1 LU 504482 B1 LU504482 B1 LU 504482B1 LU 504482 A LU504482 A LU 504482A LU 504482 A LU504482 A LU 504482A LU 504482 B1 LU504482 B1 LU 504482B1
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- C07C45/00—Preparation of compounds having >C = O groups bound only to carbon or hydrogen atoms; Preparation of chelates of such compounds
- C07C45/78—Separation; Purification; Stabilisation; Use of additives
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
- C07C45/00—Preparation of compounds having >C = O groups bound only to carbon or hydrogen atoms; Preparation of chelates of such compounds
- C07C45/78—Separation; Purification; Stabilisation; Use of additives
- C07C45/79—Separation; Purification; Stabilisation; Use of additives by solid-liquid treatment; by chemisorption
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- C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
- C07C45/00—Preparation of compounds having >C = O groups bound only to carbon or hydrogen atoms; Preparation of chelates of such compounds
- C07C45/78—Separation; Purification; Stabilisation; Use of additives
- C07C45/80—Separation; Purification; Stabilisation; Use of additives by liquid-liquid treatment
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- C07—ORGANIC CHEMISTRY
- C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
- C07C45/00—Preparation of compounds having >C = O groups bound only to carbon or hydrogen atoms; Preparation of chelates of such compounds
- C07C45/78—Separation; Purification; Stabilisation; Use of additives
- C07C45/86—Use of additives, e.g. for stabilisation
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- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
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Abstract
The present application provides a method for extracting and separating zingerone from ginger, which is implemented using ginger as a raw material, by respectively performing steps of pre-treatment, ultrasonic extraction, ultrafiltration, purification and vacuum concentration. In the ultrasonic extraction process, sodium dodecyl sulfate and L-cysteine are further added to ginger. The mass ratio of ginger to L-cysteine is 100:3-6. Sodium dodecyl sulfate is prepared into solution with mass fraction of G.5%-1.0% using purified water. The mass ratio of ginger to sodium dodecyl sulfate is 1:1-3. According to the present application., the extraction transfer rate is as high as 79.1%. Due to the introduction of L-cysteine that is dissolved in water and is weakly acidic, combination with its specific addition method, and the presence of sodium dodecyl sulfate, the whole extraction system is still weakly alkaline under the presence of L-cysteine, which is more beneficial to the dissolution of zingerone. According to the present application, zingerone is not degraded in the extraction process and the purity of the extracted zingerone is more than 98%.
Description
METHOD FOR EXTRACTING AND SEPARATING ZINGERONE FROM
GINGER
The present application relates to the field of food technology, in particular to a method for extracting and separating zingerone from ginger.
Ginger contains zingerone, which can be regarded as a homolog of capsaicin and has anesthetic, cooling and antiemetic effects. It can be used as a spice and stomach tonic.
Zingerone in ginger inhibits the synthesis of prostaglandins, especially pge: and leukotrienes, and has analgesic, antipyretic and anti-inflammatory effects to a certain extent. Zingerone, having a scientific name 4-(4-hydroxy-3-methoxyphenyl) -2-butanone, appears as a white to light yellow crystal. Zingerone is a yellow to brownish yellow liquid or crystal solid, with a melting point of 40°C. it has a strong ginger-like pungent odor and a ginger-like pungent taste. At present, the methods for extracting zingerone from ginger mainly include steam distillation, solvent extraction, pressing, carbon dioxide supercritical extraction, etc. However, there are still some technical problems in these methods, such as incomplete extraction of zingerone, low transfer rate, easiness in oxidation and degradation of zingerone in the extraction process, and low purity, which need to be solved urgently.
The purpose of the present application is to provide a method for extracting and separating zingerone from ginger, which is implemented by adopting the following technical solution:
A method for extracting and separating zingerone from ginger, wherein the method for extracting and separating zingerone from ginger is implemented using ginger as a 0006482 raw material, by respectively performing steps of pre-treatment, ultrasonic extraction, ultrafiltration, purification and vacuum concentration; in the ultrasonic extraction process, sodium dodecyl sulfate and L-cysteine are further added to ginger; the mass ratio of ginger to L-cysteine is 100:3-6; sodium dodecyl sulfate is prepared into solution with mass fraction of 0.5%-1.0% using purified water; the mass ratio of ginger to sodium dodecyl sulfate is 1:1-3; the pre-treatment includes washing ginger with clean water, putting ginger into a crusher, performing crushing using a 10-mesh to 20-mesh sieve, and collecting ginger residues and ginger juice for future use after crushing.
In order to improve the transfer rate of zingerone in the extraction process and ensure that zingerone is not oxidized and degraded in the extraction process, the ultrasonic extraction is performed according to the following steps: (1) taking and putting the ginger residues and ginger juice after pre-treatment into a suitable mixing vessel with a stirrer, adding L-cysteine, setting the rotating speed to 10-20rpm, and performing stirring for mixing for 20-30min to obtain a mixture for future use; (2) taking sodium dodecyl sulfate, and adding purified water for dissolution to obtain sodium dodecyl sulfate solution, wherein the mass fraction of sodium dodecyl sulfate solution is 0,7%-0.9%; and (3) adding the sodium dodecyl sulfate solution in step (2) to the mixture in step (1), then adding ethanol solution with volume fraction of 70%-80%, performing uniform mixing, putting the mixture into an ultrasonic extraction instrument, performing ultrasonic extraction for 30-50min, taking out the mixture, performing filtration, and collecting filtrate to obtain ultrasonic extraction solution for future use, wherein the mass ratio of the sodium dodecyl sulfate solution to the 70%-80% ethanol solution is 1:1-2, and the ultrasonic extraction frequency is 20-40KHZ.
In the entire ultrasonic extraction process, L-cysteine has antioxidant properties. In combination with the specific addition method of L-cysteine, it can mimic the 0006482 oxidation of zingerone by air in the ultrasonic extraction process. However,
L-cysteine becomes weakly acidic when dissolved in water, and the solubility of zingerone decreases under acidic conditions, which reduces the transfer rate of zingerone in the extraction process. As a surfactant, sodium dodecyl sulfate, in combination with the dilute alcohol system of the entire extraction system and specific ultrasound parameters, can improve the transfer rate of zingerone in the ultrasonic extraction process, On the other hand, sodium dodecyl sulfate becomes alkaline after dissolved in water. The specific amount of sodium dodecyl sulfate and
L-cysteine, in combination with the specific addition method of the two, can make the entire ultrasonic extraction system weakly alkaline, which is more suitable for the dissolution of zingerone. Ultimately, zingerone is prevented from being oxidized and degraded in the extraction process, and the transfer rate of zingerone is improved.
Further, the ultrafiltration includes performing ultrafiltration treatment on the ultrasonic extraction solution, and specifically includes performing ultrafiltration using a ceramic membrane with molecular weight cutoff of 8000-12000, setting the pressure to 0.6-0.9MPa and the ultrafiltration temperature to 25-30°C, collecting ultrafiltration solution after ultrafiltration, putting the ultrafiltration solution into a rotary evaporator, setting the temperature to 30-35°C and the vacuum degree to -0.06 to -0.08MPa, and performing vacuum concentration to obtain an extract with relative density of 1.08-1.12 (30°C).
Further, the purification includes performing adsorption on the obtained extract using macroporous adsorption resin, firstly performing elution using dilute hydrochloric acid solution with pH of 5.0-6.0 at a flow rate of 1-3mL/min, then performing elution using purified water at a flow rate of I-3mL/min until eluent becomes neutral, then performing elution using ethanol solution with volume fraction of 45%-55% at a flow rate of 1-3mL/min, and then collecting ethanol cluate for future use; the mass ratio of the macroporous adsorption resin to the extract is 10-15:1, the mass ratio of the dilute hydrochloric acid solution to the extract is 5-8:1, and the mass ratio of the ethanol solution to the extract is 15-18:1. 0006482
Further, the vacuum concentration includes taking and putting the obtained ethanol eluate into the rotary evaporator, setting the temperature to 30-35°C and the vacuum degree to -0.06 to -0.08MPa, recovering ethanol, and performing concentration until the relative density is 1.136-1.138 (25°C) to obtain product zingerone.
The present application has the following beneficial effects:
In the method for extracting and separating zingerone from ginger provided in the present application, by using the extraction solution with specific ethanol concentration and combining with the addition of surfactant sodium dodecyl sulfate and the specific extraction and purification method, the extraction transfer rate can be as high as 79.1%. The introduction of L-cysteine which is weakly acidic after dissolved in water can prevent zingerone from being oxidized and degraded in the extraction and purification process. Although this will lead to a decrease in the pH of the entire extraction system solution due to the weakly acidic nature of L-cysteine, which ultimately leads to a decrease in the extraction transfer rate of zingerone, due to the alkaline nature of sodium dodecyl sulfate, the entire extraction system remains weakly alkaline in the presence of L-cysteine. In combination with the specific purification method, the transfer rate in the entire extraction and purification process can be ensured to be high, while it is prevented from being oxidized and degraded throughout the process, thus making the purity of the extracted and purified zingerone reach more than 98%. The method in the present application is simple and feasible, and is easy to popularize and apply.
The present application will be specifically described below through examples. The following examples are only for further description of the present application and cannot be understood as limitations on the scope of protection of the present application. Those skilled in the art may make some non-essential improvements and adjustments to the present application based on the content of the present application.
Example 1
À method for extracting and separating zingerone from ginger was implemented according to the following steps: 1. Pre-treatment
Ginger was washed with clean water and put into a crusher. Crushing was performed using a 20-mesh sieve. 100 parts of ginger residues and ginger juice after crushing were collected for future use. 2. Ultrasonic extraction: (1) The ginger residues and ginger juice after pre-treatment were taken and put into a suitable mixing vessel with a stirrer. 6 parts of L-cysteine were added. The rotating speed was set to 20rpm. Stirring was performed for mixing for 30min to obtain a mixture fur future use. (2) Sodium dodecyl sulfate was taken. Purified water was added for dissolution to obtain sodium dodecyl sulfate solution. The mass fraction of the sodium dodecyl sulfate solution was preferably 0.9%. The mass ratio of the ginger to the sodium dodecyl sulfate solution was 1:2. (3) The sodium dodecyl sulfate solution in step (2) was added to the mixture in step (1). Then ethanol solution with volume fraction of 80% was added. Uniform mixing was performed. The mixture was put into an ultrasonic extraction instrument.
Ultrasonic extraction was performed for 50min. The mixture was taken out. Filtration was performed. Filtrate was collected to obtain ultrasonic extraction solution for future use. The mass ratio of the sodium dodecyl sulfate solution to the 80% ethanol solution was 1:2. The ultrasonic extraction frequency was 40KHZ. 3. Ultrafiltration:
Ultrafiltration treatment was performed on the obtained ultrasonic extraction solution.
Specifically, ultrafiltration was performed using a ceramic membrane with molecular weight cutoff of 8000-12000. The pressure was set to 0.8MPa. The ultrafiltration
+ ; . . LU504482 temperature was set to 28°C. Ultrafiltration solution was collected after ultrafiltration.
The ultrafiltration solution was put into a rotary evaporator. The temperature was set to 32°C. The vacuum degree was set to -0.07MPa. Vacuum concentration was performed to obtain an extract with relative density of 1.11 (30°C). 4. Purification:
Adsorption was performed on the obtained extract using macroporous adsorption resin. Firstly, elution was performed using dilute hydrochloric acid solution with pH of 5,52 at a flow rate of 2mL/min. Then, elution was performed using purified water at a flow rate of 2mL/min until eluent becomes neutral. Then, elution was performed using ethanol solution with volume fraction of 55% at a flow rate of 2mL/min. Then, ethanol cluate was collected for future use. The mass ratio of the macroporous adsorption resin to the extract was 15:1. The mass ratio of the dilute hydrochloric acid solution to the extract was 8:1. The mass ratio of the ethanol solution to the extract was 18:1. 5. Vacuum concentration:
The obtained ethanol eluate was taken and put into the rotary evaporator. The temperature was set to 32°C. The vacuum degree was set to -0.07MPa. Ethanol was recovered. Concentration was performed until the relative density was 1.138 (25°C) to obtain product zingerone. After testing, the transfer rate of zingerone in example 1 was 77.2%, and the purity of the obtained product zingerone was 98.3%.
Example 2
A method for extracting and separating zingerone from ginger was implemented according to the following steps: 1. Pre-treatment
Ginger was washed with clean water and put into a crusher. Crushing was performed using a 16-mesh sieve, 100 parts of ginger residues and ginger juice after crushing were collected for future use.
. . LU504482 2. Ultrasonic extraction: (1) The ginger residues and ginger juice after pre-treatment were taken and put into a suitable mixing vessel with a stirrer. 5 parts of L-cysteine were added. The rotating speed was set to 16rpm. Stirring was performed for mixing for 25min to obtain a mixture fur future use. (2) Sodium dodecyl sulfate was taken. Purified water was added for dissolution to obtain sodium dodecyl sulfate solution. The mass fraction of the sodium dodecyl sulfate solution was preferably 0.8%. The mass ratio of the ginger to the sodium dodecyl sulfate solution was 1:2. (3) The sodium dodecyl sulfate solution in step (2) was added to the mixture in step (1). Then ethanol solution with volume fraction of 75% was added. Uniform mixing was performed. The mixture was put into an ultrasonic extraction instrument.
Ultrasonic extraction was performed for 40min. The mixture was taken out. Filtration was performed. Filtrate was collected to obtain ultrasonic extraction solution for future use. The mass ratio of the sodium dodecyl! sulfate solution to the 75% ethanol solution was 1:1. The ultrasonic extraction frequency was 30KHZ. 3. Ultrafiltration:
Ultrafiltration treatment was performed on the obtained ultrasonic extraction solution.
Specifically, ultrafiltration was performed using a ceramic membrane with molecular weight cutoff of 8000-12000. The pressure was set to 0.8MPa. The ultrafiltration temperature was set to 28°C. Ultrafiltration solution was collected after ultrafiltration.
The ultrafiltration solution was put into a rotary evaporator. The temperature was set to 32°C. The vacuum degree was set to -0.07MPa. Vacuum concentration was performed to obtain an extract with relative density of 1.10 (30°C). 4. Purification:
Adsorption was performed on the obtained extract using macroporous adsorption resin. Firstly, elution was performed using dilute hydrochloric acid solution with pH pe . . , LU504482 of 5.59 at a flow rate of 2mL/min. Then, clution was performed using purified water at a flow rate of 2mL/min until eluent becomes neutral. Then, elution was performed using ethanol solution with volume fraction of 50% at a flow rate of 2mlL/min. Then, ethanol eluate was collected for future use. The mass ratio of the macroporous adsorption resin to the extract was 12:1. The mass ratio of the dilute hydrochloric acid solution to the extract was 6:1. The mass ratio of the ethanol solution to the extract was 17:1. 5. Vacuum concentration:
The obtained ethanol cluate was taken and put into the rotary evaporator. The temperature was set to 32°C. The vacuum degree was set to -0.07MPa. Ethanol was recovered. Concentration was performed until the relative density was 1.137 (25°C) to obtain product zingerone. The transfer rate of zingerone in example 2 was 75.2%, and the purity of the obtained product zingerone was 99.1%.
Example 3
A method for extracting and separating zingerone from ginger was implemented according to the following steps: . Pre-treatment
Ginger was washed with clean water and put into a crusher. Crushing was performed using a 10-mesh sieve. 100 parts of ginger residues and ginger juice after crushing were collected for future use. 2. Ultrasonic extraction: (1) The ginger residues and ginger juice after pre-treatment were taken and put into a suitable mixing vessel with a stirrer. 3 parts of L-cysteine were added. The rotating speed was set to 10rpm. Stirring was performed for mixing for 30min to obtain a mixture fur future use. (2) Sodium dodecyl sulfate was taken. Purified water was added for dissolution to obtain sodium dodecyl sulfate solution. The mass fraction of the sodium dodecyl
; . . . LU504482 sulfate solution was preferably 0.7%. The mass ratio of the ginger to the sodium dodecyl sulfate solution was 1:3. (3) The sodium dodecyl sulfate solution in step (2) was added to the mixture in step (1). Then ethanol solution with volume fraction of 70% was added. Uniform mixing was performed. The mixture was put into an ultrasonic extraction instrument.
Ultrasonic extraction was performed for 30min. The mixture was taken out. Filtration was performed. Filtrate was collected to obtain ultrasonic extraction solution for future use. The mass ratio of the sodium dodecyl sulfate solution to the 70% ethanol solution was 1:1. The ultrasonic extraction frequency was 20KHZ. 3. Ultrafiltration:
Ultrafiltration treatment was performed on the obtained ultrasonic extraction solution.
Specifically, ultrafiltration was performed using a ceramic membrane with molecular weight cutoff of 8000-12000. The pressure was set to 0.6MPa. The ultrafiltration temperature was set to 25°C. Ultrafiltration solution was collected after ultrafiltration.
The ultrafiltration solution was put into a rotary evaporator. The temperature was set to 30°C. The vacuum degree was set to -0.06MPa. Vacuum concentration was performed to obtain an extract with relative density of 1.08 (30°C). 4. Purification:
Adsorption was performed on the obtained extract using macroporous adsorption resin. Firstly, elution was performed using dilute hydrochloric acid solution with pH of 5.06 at a flow rate of ImL/min. Then, elution was performed using purified water at a flow rate of ImL/min until eluent becomes neutral. Then, elution was performed using ethanol solution with volume fraction of 45% at a flow rate of ml/min. Then, ethanol eluate was collected for future use. The mass ratio of the macroporous adsorption resin to the extract was 10:1. The mass ratio of the dilute hydrochloric acid solution to the extract was 5:1. The mass ratio of the cthanol solution to the extract was 15:1, 5. Vacuum concentration:
‘ ; LU504482
The obtained ethanol eluate was taken and put into the rotary evaporator. The temperature was set to 30°C. The vacuum degree was set to -0.06MPa. Ethanol was recovered. Concentration was performed until the relative density was 1.136 (25°C) to obtain product zingerone. The transfer rate of zingerone in example 3 was 79.1%, and the purity of the obtained product zingerone was 98.1%.
Example 4
A method for extracting and separating zingerone from ginger was implemented according to the following steps (according to the method in example 1, but no
L-cysteine and no sodium dodecyl sulfate were added in the preparation process): 1. Pre-treatment
Ginger was washed with clean water and put into a crusher. Crushing was performed using a 20-mesh sieve. 100 parts of ginger residues and ginger juice after crushing were collected for future use. 2. Ultrasonic extraction: (1) The ginger residues and ginger juice after pre-treatment were taken and put into a suitable mixing vessel with a stirrer. The rotating speed was set to 20rpm. Stirring was performed for mixing for 30min to obtain a mixture fur future use. (2) Purified water was added to the mixture in step (1). Then ethanol solution with volume fraction of 80% was added. Uniform mixing was performed. The mixture was put into an ultrasonic extraction instrument. Ultrasonic extraction was performed for 50min. The mixture was taken out. Filtration was performed. Filtrate was collected to obtain ultrasonic extraction solution for future use. The mass ratio of the ginger to the purified water was 1:2. The mass ratio of the purified water to the 80% ethanol solution was 1:2. The ultrasonic extraction frequency was 40KHZ. 3. Ultrafiltration:
Ultrafiltration treatment was performed on the obtained ultrasonic extraction solution.
Specifically, ultrafiltration was performed using a ceramic membrane with molecular weight cutoff of 8000-12000. The pressure was set to 0.8MPa. The ultrafiltration 0006482 temperature was set to 28°C. Ultrafiltration solution was collected after ultrafiltration.
The ultrafiltration solution was put into a rotary evaporator. The temperature was set to 32°C. The vacuum degree was set to -0.07MPa. Vacuum concentration was performed to obtain an extract with relative density of 1.11 (30°C). 4. Purification:
Adsorption was performed on the obtained extract using macroporous adsorption resin. Firstly, elution was performed using dilute hydrochloric acid solution with pH of 5.52 at a flow rate of 2mL/min. Then, elution was performed using purified water at a flow rate of 2mL/min until eluent becomes neutral. Then, elution was performed using ethanol solution with volume fraction of 55% at a flow rate of 2mL/min, Then, ethanol eluate was collected for future use. The mass ratio of the macroporous adsorption resin to the extract was 15:1. The mass ratio of the dilute hydrochloric acid solution to the extract was 8:1. The mass ratio of the ethanol solution to the extract was 18:1. 5. Vacuum concentration:
The obtained ethanol eluate was taken and put into the rotary evaporator. The temperature was set to 32°C. The vacuum degree was set to -0.07MPa. Ethanol was recovered. Concentration was performed until the relative density was 1.138 (25°C) to obtain product zingerone. After testing, the transfer rate of zingerone in example 4 was 62.3%, and the purity of the obtained product zingerone was 93.7%.
From example 4, it can be seen that in the absence of L-cysteine and sodium dodecyl sulfate, the transfer rate of zingerone is significantly lower than that in examples 1, 2 and 3, and the purity is also significantly lower than that m examples 1, 2 and 3.
Therefore, in the present application, the addition of L-cysteine and sodium dodecyl sulfate can improve the purity of zingerone while improving the transfer rate of zingerone,
Claims (4)
- CLAIMS . A method for extracting and separating zingerone from ginger, wherein the method for extracting and separating zingerone from ginger is implemented using ginger as a raw material, by respectively performing steps of pre-treatment, ultrasonic extraction, ultrafiltration, purification and vacuum concentration; in the ultrasonic extraction process, sodium dodecyl sulfate and L-cysteine are further added to ginger; the mass ratio of ginger to L-cysteine is 100:3-6; sodium dodecyl sulfate is prepared into solution with mass fraction of 0.5%-1.0% using purified water; the mass ratio of ginger to sodium dodecyl sulfate is 1:1-3; the pre-treatment comprises washing ginger with clean water, putting ginger into a crusher, performing crushing using a 10-mesh to 20-mesh sieve, and collecting ginger restdues and ginger juice for future use after crushing; the ultrasonic extraction is performed according to the following steps: (1) taking and putting the ginger residues and ginger juice after pre-treatment into a suitable mixing vessel with a stirrer, adding L-cysteine, setting the rotating speed to 10-20rpm, and performing stirring for mixing for 20-30min to obtain a mixture for future use: (2) taking sodium dodecyl sulfate, and adding purified water for dissolution to obtain sodium dodecyl sulfate solution, wherein the mass fraction of sodium dodecyl! sulfate solution is 0.7%-0.9%; and (3) adding the sodium dodecyl sulfate solution in step (2) to the mixture in step (1), then adding ethanol solution with volume fraction of 70%-80%, performing uniform mixing, putting the mixture into an ultrasonic extraction instrument, performing ultrasonic extraction for 30-50min, taking out the mixture, performing filtration, and collecting filtrate to obtain ultrasonic extraction solution for future use, wherein the mass ratio of the sodium dodecyl sulfate solution to the 70%-80% ethanol solution is 1:1-2, and the ultrasonic extraction frequency is 20-40KHZ.
- 2. The method for extracting and scparating zingerone from ginger according to 0006482 claim 1, wherein the ultrafiltration comprises performing ultrafiltration treatment on the ultrasonic extraction solution, and specifically comprises performing ultrafiltration using a ceramic membrane with molecular weight cutoff of 8000-12000, setting the pressure to 0.6-0.9MPa and the ultrafiltration temperature to 25-30°C, collecting ultrafiltration solution after ultrafiltration, putting the ultrafiltration solution into a rotary evaporator, setting the temperature to 30-35°C and the vacuum degree to -0.06 to -0.08MPa, and performing vacuum concentration to obtain an extract with relative density of 1.08-1.12 (30°C).
- 3. The method for extracting and separating zingerone from ginger according to claim 2, wherein the purification comprises performing adsorption on the obtained extract using macroporous adsorption resin, firstly performing elution using dilute hydrochloric acid solution with pH of 5.0-6.0 at a flow rate of 1-3mL/min, then performing elution using purified water at a flow rate of 1-3mL/min until eluent becomes neutral, then performing elution using ethanol solution with volume fraction of 45%-55% at a flow rate of 1-3mL/min, and then collecting ethanol eluate for future use; the mass ratio of the macroporous adsorption resin to the extract is 10-15:1, the mass ratio of the dilute hydrochloric acid solution to the extract is 5-8:1, and the mass ratio of the ethanol solution to the extract is 15-18:1.
- 4. The method for extracting and separating zingerone from ginger according to claim 3, wherein the vacuum concentration comprises taking and putting the obtained ethanol eluate into the rotary evaporator, setting the temperature to 30-35°C and the vacuum degree to -0.06 to -0.08MPa, recovering ethanol, and performing concentration until the relative density is 1.136-1.138 (25°C) to obtain product zingerone.
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| CN103012095B (en) * | 2012-12-06 | 2014-07-30 | 山东农业大学 | Method for combined extraction of variety of functional components of ginger |
| JP5829225B2 (en) * | 2013-02-07 | 2015-12-09 | 長谷川香料株式会社 | Method for producing ginger extract with high gingerol content |
| CN108048432B (en) * | 2018-01-30 | 2020-08-07 | 湖南科技学院 | Ginger protease extraction method |
| CN108355115A (en) * | 2018-04-11 | 2018-08-03 | 山东农业大学 | A kind of method of continuous extraction purification ginger polyphenol |
| CN109096079A (en) * | 2018-11-05 | 2018-12-28 | 福建拓天生物科技有限公司 | A kind of production technology of rapidly extracting curcumin |
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| CN113387785A (en) * | 2021-07-05 | 2021-09-14 | 中国科学院烟台海岸带研究所 | Method for extracting curcumin from turmeric with assistance of biological enzyme method combined with continuous flow ultrasonic |
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