LU504365B1 - Application of luteolin in inhibiting the postharvest diseases of citrus fruit - Google Patents
Application of luteolin in inhibiting the postharvest diseases of citrus fruit Download PDFInfo
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- LU504365B1 LU504365B1 LU504365A LU504365A LU504365B1 LU 504365 B1 LU504365 B1 LU 504365B1 LU 504365 A LU504365 A LU 504365A LU 504365 A LU504365 A LU 504365A LU 504365 B1 LU504365 B1 LU 504365B1
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- MWDZOUNAPSSOEL-UHFFFAOYSA-N kaempferol Natural products OC1=C(C(=O)c2cc(O)cc(O)c2O1)c3ccc(O)cc3 MWDZOUNAPSSOEL-UHFFFAOYSA-N 0.000 title claims abstract description 64
- LRDGATPGVJTWLJ-UHFFFAOYSA-N luteolin Natural products OC1=CC(O)=CC(C=2OC3=CC(O)=CC(O)=C3C(=O)C=2)=C1 LRDGATPGVJTWLJ-UHFFFAOYSA-N 0.000 title claims abstract description 64
- IQPNAANSBPBGFQ-UHFFFAOYSA-N luteolin Chemical compound C=1C(O)=CC(O)=C(C(C=2)=O)C=1OC=2C1=CC=C(O)C(O)=C1 IQPNAANSBPBGFQ-UHFFFAOYSA-N 0.000 title claims abstract description 64
- 235000009498 luteolin Nutrition 0.000 title claims abstract description 64
- 235000020971 citrus fruits Nutrition 0.000 title claims abstract description 39
- 201000010099 disease Diseases 0.000 title claims abstract description 25
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 title claims abstract description 25
- 230000002401 inhibitory effect Effects 0.000 title claims abstract description 24
- 241000207199 Citrus Species 0.000 claims abstract description 23
- 230000004580 weight loss Effects 0.000 claims abstract description 10
- 239000003755 preservative agent Substances 0.000 claims description 13
- 230000002335 preservative effect Effects 0.000 claims description 13
- 241000122123 Penicillium italicum Species 0.000 claims description 9
- 235000002233 Penicillium roqueforti Nutrition 0.000 claims description 9
- 238000000034 method Methods 0.000 claims description 8
- 241000595503 Geotrichum citri-aurantii Species 0.000 claims description 5
- 235000013399 edible fruits Nutrition 0.000 abstract description 41
- 241000675108 Citrus tangerina Species 0.000 abstract description 12
- 239000007787 solid Substances 0.000 abstract description 3
- 230000008859 change Effects 0.000 abstract description 2
- 238000002791 soaking Methods 0.000 abstract description 2
- 240000002319 Citrus sinensis Species 0.000 description 20
- 235000005976 Citrus sinensis Nutrition 0.000 description 20
- 230000000694 effects Effects 0.000 description 11
- 230000003902 lesion Effects 0.000 description 11
- 238000011081 inoculation Methods 0.000 description 7
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 6
- 238000002474 experimental method Methods 0.000 description 5
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 5
- 230000008506 pathogenesis Effects 0.000 description 4
- 239000000417 fungicide Substances 0.000 description 3
- 239000001963 growth medium Substances 0.000 description 3
- RAXXELZNTBOGNW-UHFFFAOYSA-N imidazole Natural products C1=CNC=N1 RAXXELZNTBOGNW-UHFFFAOYSA-N 0.000 description 3
- 238000001727 in vivo Methods 0.000 description 3
- 239000000463 material Substances 0.000 description 3
- 239000000725 suspension Substances 0.000 description 3
- 239000005708 Sodium hypochlorite Substances 0.000 description 2
- 241000607479 Yersinia pestis Species 0.000 description 2
- 239000012153 distilled water Substances 0.000 description 2
- 230000003203 everyday effect Effects 0.000 description 2
- 229930003935 flavonoid Natural products 0.000 description 2
- 150000002215 flavonoids Chemical class 0.000 description 2
- 235000017173 flavonoids Nutrition 0.000 description 2
- 239000002054 inoculum Substances 0.000 description 2
- SUKJFIGYRHOWBL-UHFFFAOYSA-N sodium hypochlorite Chemical compound [Na+].Cl[O-] SUKJFIGYRHOWBL-UHFFFAOYSA-N 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 231100000331 toxic Toxicity 0.000 description 2
- 230000002588 toxic effect Effects 0.000 description 2
- HYZJCKYKOHLVJF-UHFFFAOYSA-N 1H-benzimidazole Chemical compound C1=CC=C2NC=NC2=C1 HYZJCKYKOHLVJF-UHFFFAOYSA-N 0.000 description 1
- OUXCBPLFCPMLQZ-WOPPDYDQSA-N 4-amino-1-[(2r,3s,4s,5r)-4-hydroxy-5-(hydroxymethyl)-3-methyloxolan-2-yl]-5-iodopyrimidin-2-one Chemical compound C[C@H]1[C@H](O)[C@@H](CO)O[C@H]1N1C(=O)N=C(N)C(I)=C1 OUXCBPLFCPMLQZ-WOPPDYDQSA-N 0.000 description 1
- 208000024172 Cardiovascular disease Diseases 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 230000004913 activation Effects 0.000 description 1
- 238000007605 air drying Methods 0.000 description 1
- 230000001093 anti-cancer Effects 0.000 description 1
- 230000003064 anti-oxidating effect Effects 0.000 description 1
- 230000000259 anti-tumor effect Effects 0.000 description 1
- 239000003963 antioxidant agent Substances 0.000 description 1
- 235000006708 antioxidants Nutrition 0.000 description 1
- 150000004283 biguanides Chemical class 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 238000003306 harvesting Methods 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 238000012423 maintenance Methods 0.000 description 1
- 239000002609 medium Substances 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 244000000010 microbial pathogen Species 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 244000052769 pathogen Species 0.000 description 1
- 150000008442 polyphenolic compounds Chemical class 0.000 description 1
- 235000013824 polyphenols Nutrition 0.000 description 1
- 230000008092 positive effect Effects 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 239000008223 sterile water Substances 0.000 description 1
- 208000024891 symptom Diseases 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
Classifications
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23B—PRESERVING, e.g. BY CANNING, MEAT, FISH, EGGS, FRUIT, VEGETABLES, EDIBLE SEEDS; CHEMICAL RIPENING OF FRUIT OR VEGETABLES; THE PRESERVED, RIPENED, OR CANNED PRODUCTS
- A23B7/00—Preservation or chemical ripening of fruit or vegetables
- A23B7/14—Preserving or ripening with chemicals not covered by groups A23B7/08 or A23B7/10
- A23B7/153—Preserving or ripening with chemicals not covered by groups A23B7/08 or A23B7/10 in the form of liquids or solids
- A23B7/154—Organic compounds; Microorganisms; Enzymes
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
- A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
- A01N43/14—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings
- A01N43/16—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings with oxygen as the ring hetero atom
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01P—BIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
- A01P3/00—Fungicides
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L3/00—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs
- A23L3/34—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs by treatment with chemicals
- A23L3/3454—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs by treatment with chemicals in the form of liquids or solids
- A23L3/3463—Organic compounds; Microorganisms; Enzymes
- A23L3/3481—Organic compounds containing oxygen
- A23L3/3499—Organic compounds containing oxygen with doubly-bound oxygen
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- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Health & Medical Sciences (AREA)
- Polymers & Plastics (AREA)
- Food Science & Technology (AREA)
- Pest Control & Pesticides (AREA)
- Plant Pathology (AREA)
- Environmental Sciences (AREA)
- Nutrition Science (AREA)
- Agronomy & Crop Science (AREA)
- Dentistry (AREA)
- General Health & Medical Sciences (AREA)
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- Medicines Containing Plant Substances (AREA)
Abstract
The invention discloses an application of luteolin in inhibiting the postharvest diseases of citrus fruit, and belongs to the technical field of citrus postharvest disease control. The invention finds that soaking citrus fruits with luteolin solution can inhibit the decay rate of citrus fruits and delay weight loss. It was also found that luteolin could delay the color change of citrus and increase the soluble solids content, which indicated that luteolin could delay the diseases occurrence and weight loss of Nanfeng tangerine fruit and maintain high quality.
Description
APPLICATION OF LUTEOLIN IN INHIBITING THE POSTHARVEST
DISEASES OF CITRUS FRUIT
The invention relates to the technical field of citrus postharvest disease control, in particular to the application of luteolin in inhibiting the postharvest diseases of citrus fruit.
Citrus is the largest fruit in China and the world, its physiological metabolism is vigorous after harvest, so it is infected by pathogenic microorganisms easily and causing loses of commodity. These diseases mainly refer to blue mold caused by Penicillium italicum and sour rot caused by Geotrichum citri-aurantii. At present, the main fungicides used to control postharvest diseases of citrus in the market are benzimidazole, imidazole and biguanide salts.
These chemical synthetic fungicides have the certain inhibitory effects on postharvest pathogens of citrus fruit, but they are toxic. Considering food safety and environmental safety, its use has been restricted in many countries. Therefore, some alternative plant antioxidants (such as polyphenols and flavonoids) have been studied to replace chemical fungicides in order to avoid toxic residues in edible parts of citrus and reduce decay.
Luteolin is a natural flavonoid, which has positive effects on human health, such as anti- oxidation, anti-tumor, anti-cancer and treatment of cardiovascular diseases.
The purpose of the invention is to provide the application of luteolin in inhibiting the postharvest diseases of citrus, so as to solve the problems existing in the current technology.
In order to achieve the above purposes, the present invention provides the following scheme:
The invention provides a preservative for inhibiting postharvest diseases of citrus, which comprises luteolin.
Preferably, the diseases include blue mold and/or sour rot.
Preferably, the blue mold refers to the blue mold caused by Penicillium italicum.
Preferably, the sour rot refers to the sour rot caused by Geotrichum citri-aurantii.
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Preferably, the concentration of luteolin solution is not less than 3 g/L.
The invention also provides a method for inhibiting postharvest diseases of citrus, wherein, citrus fruits are soaked in the preservative for 3-7 min, and air-dried thereafter.
The invention also provides the application of luteolin in inhibiting the mycelial growth of Penicillium italicum.
The invention also provides the application of luteolin in inhibiting the mycelial growth of Geotrichum citri-aurantii.
The invention also provides the application of luteolin in delaying postharvest decay of citrus.
The invention also provides the application of luteolin in delaying the postharvest weight loss of citrus.
The invention discloses the following technical effects:
The invention provides a safe and low-cost method for inhibiting postharvest diseases of citrus, which prolongs the fresh-keeping period of citrus, reduces decay loss and improves economic benefits. The invention finds that soaking citrus fruits with luteolin solution can inhibit the decay rate of citrus fruits and delay weight loss. It was also found that luteolin could delay the color change of citrus and increase total soluble solids content, which indicated that luteolin could delay the diseases occurrence and weight loss of Nanfeng tangerine fruit and maintain high quality.
In order to explain the embodiments of the present invention or the technical scheme in the current technology more clearly, the figures needed in the embodiments will be introduced below briefly. Obviously, the figures described below are only some embodiments of the present invention, and other figures can be obtained according to these figures without creative work for ordinary people in the field.
Fig. 1 shows the inhibitory effects of luteolin with different concentrations on the mycelial growth of G.citri-aurantii.
Fig. 2 shows the effects of luteolin on the pathogenesis of the sour rot of ‘Newhall’ navel orange (citrus sinensis (L.) Osbeck fruit.
Fig. 3 shows the effects of luteolin on the pathogenesis of ‘Newhall’ navel orange fruit and Nanfeng tangerine fruit; wherein, (A) observation of diseased symptoms on the 3™ day after inoculation; (B) the lesion diameter of ‘Newhall’ navel orange fruit, (C) the lesion 565 diameter of the Nanfeng tangerine.
Fig. 4 shows the effects of luteolin treatment on citrus fruit quality and decay.
A number of exemplary embodiments of the present invention will now be described in detail, and this detailed description should not be considered as a limitation of the present invention, but should be understood as a more detailed description of certain aspects, characteristics and embodiments of the present invention.
It should be understood that the terminology described in the present invention is only for describing specific embodiments and is not used to limit the present invention. In addition, for the numerical range in the present invention, it should be understood that each intermediate value between the upper limit and the lower limit of the range is also disclosed specifically.
The intermediate value within any stated value or stated range and every smaller range between any other stated value or intermediate value within the stated range are also included in the present invention. The upper and lower limits of these smaller ranges can be included or excluded from the range independently.
Unless otherwise specified, all technical and scientific terms used herein have the same meaning as understood commonly by one of ordinary skill in the field to which this invention relates. Although the present invention only describes the preferred methods and materials, any methods and materials similar or equivalent to those described herein can also be used in the practice or testing of the present invention. All documents mentioned in this specification are incorporated by reference to disclose and describe methods and/or materials related to the documents. In case of conflict with any incorporated document, the contents of this specification shall prevail.
It is obvious to those skilled in the field that many improvements and changes can be made to the specific embodiments of the present invention without departing from the scope or spirit of the present invention. Other embodiments will be apparent to the skilled person from the description of the invention. The specification and example of this application are only exemplary.
The terms "include", "comprise", "have" and "contain" used in this article are all open terms, which means including but not limited to.
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Unless otherwise specified, the technical scheme described in this invention is a conventional scheme in this field, and the reagents or raw materials used are all purchased from commercial channels or have been disclosed.
Embodiment 1 Inhibitory experiment of luteolin with different concentrations on G.citri- aurantii of ‘Newhall’ navel orange.
In this embodiment, the inhibitory effects of luteolin with different concentrations on the mycelial growth of G.citri-aurantii of ‘Newhall’ navel orange was determined in PDA culture medium. 1. Strain activation. The strains of G.citri-aurantii stored in the ultra-low temperature refrigerator at -80°C were taken out, and 5 pL. of them were sucked on PDA culture medium with a pipette, cultured at 28°C for 5 d, and subcultured for later use. 2. PDA was used to prepare luteolin solution (dissolved by adding absolute ethanol) to make its final concentration be 0.125 g/L, 0.25 g/L, 0.5 g/L, 1 g/L, 2 g/L and 3 g/L, and it was repeated three times with PDA added absolute ethanol as the control. Puncher was used to get the inoculum disc of G.citri-aurantii with a diameter of 5 mm, and the inoculum disc was intruduced in the centre of medium and incubated at 25°C for 10 d. The lesion diameter was measured by cross method. The experiment was repeated three times.
As shown in Fig. 1, PDA culture medium containing luteolin could inhibit the growth of
G.citri-aurantii. Wherein, the G.citri-aurantii was inhibited completely when the concentration of luteolin was 3 g/L, which indicated that the minimum inhibitory concentration (MIC) of luteolin against G.citri-aurantii was 3 g/L.
Embodiment 2 Inhibitory effects of Luteolin on G.citri-aurantii of ‘Newhall’ navel orange
In this example, the effects of ‘Newhall’ navel orange fruit inoculated with G.citri-aurantii in vivo was analyzed. 1. ‘Newhall’ navel orange fruit with uniform size and free of pests and diseases were selected. All selected fruit were soaked in 2% (v/v) sodium hypochlorite solution for 2 min and then washed with distilled water and dried naturally before wounding. A uniform wound with a diameter of 5 mm and a depth of 3 mm was made with an inoculation needle at the equatorial plane of each fruit. 2. 10 pL of spore suspension concentration (1x10° spores/mL) was injected into each wound. After the wound was dried naturally, 10 uL of luteolin on the concentration of 0 g/L, 3 g/L (MIC), 15 g/L (SMIC) and 30 g/L (10MIC) was reinjected into each wound, respectively.
The inoculated fruit was placed in a sterilized fresh-keeping box, the bottom of the fruit was supported by a plastic cover, and an appropriate amount of sterile water was put in and cultured at 25°C. The incidence of fruit was observed every day, the lesion diameter inhibitory rate of the mycelial growth were measured. The experiment was repeated three times.
The statistical results of the effects of luteolin on the incidence of the sour rot of ‘Newhall’ navel orange fruit are shown in Fig. 2 and Table 1, the effects of luteolin on the lesion diameter of the sour rot in ‘Newhall’ navel orange fruit is shown in Table 2.
Table 1 Effects of luteolin on the incidence of the sour rot of ‘Newhall’ navel orange fruit
Treatmen Incidence (%) € 3d 4d 5d 6d 7d 8d 37.50+4.17 66.67+83 8750+41 9583+4.1 95.83+4.1 100.00+0.0 0 gL a 3a 74 74 74 02 33.33+0.00 70.83+4.1 91.67483 91.6783 95.83+4.1 100.00+0.0 3 gL a 74 3a 3a 74 02 29.17£4.17 66.67£0.0 87.50+4.1 9583+41 10.00+00 100.00+0.0 gL a 02 74 74 02 02 20.83+4.17 50.00£8.3 75.00£0.0 95.83+4.1 95.83+4.1 100.00+0.0 g/L b 3a 02 7a 7a 02
From Table 1, results shown that ‘Newhall’ navel orange began to get diseased after 3 days of inoculation, and there was a significant difference in the incidence of G.citri-aurantii of ‘Newhall’ navel orange fruit between the control group (0 g/L) and the treated group (30 g/L) on the third day of storage. It shows that luteolin could delay the occurrence of G.citri- aurantii and reduce the incidence of citrus fruit in vivo significantly.
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Table 2 Effects of luteolin on the lesion diameter of the sour rot of ‘Newhall’ navel orange fruit ~~ Lesiondiameter(cm)
Treatment 3d 4d 5d 6d 7d 8d © OgL 040£001° 1.54+0.05" 3.08+0.07" 427+0.06" 6.09+0.11° 7.024015" 3 g/L 0.310.025 1.52+0.10° 2.82+0.10* 3.99+0.03* 5.82+0.33* 6.99+0.27% g/L 0.20+0.01° 1.00+0.02° 2.51+0.13° 3.84+0.12° 5.52+0.06* 6.78+0.17% g/L 0.15+0.01% 0.71£0.01¢ 2.17+0.00° 3.58+0.01° 5.48+0.04* 6.79+0.118 “From Table 2, it was shown that in the in vivo inoculation experiment, 30 g/L luteolin solution could reduce the diameter of the disease spot of G.citri-aurantii significantly.
The results indicated that different concentrations of luteolin could inhibit the pathogenesis of the sour rot of ‘Newhall’ navel orange fruit (Fig. 2). Wherein, the concentration of 30 g/L has significant inhibitory effects on the incidence and lesion diameter (Tables 1 and 2). The results showed that luteolin treatment could inhibit the occurrence of sour rot in orange fruits.
Embodiment 3 Inhibitory effects of luteolin on blue mold disease of citrus fruit
The application of luteolin in inhibiting the Penicillium italicum of postharvest citrus was as follows: 1. The fruits of ‘Newhall’ navel orange and Nanfeng tangerine with the same size and without pests and diseases were selected. Fruit was soaked in 2% sodium hypochlorite solution for 2 min, washed with distilled water and dried naturally for later use. A wound with a diameter of 5 mm and a depth of 3 mm was conducted with an sterile needle on the equatorial plane of each fruit. The inoculation depth is about 2 mm. 2. The concentration of spore suspension of Penicillium italicum was 1x10” spores/mL, and the concentrations of Luteolin used in ‘Newhall’ navel orange fruit were 0 g/L(CK) and 30 g/L (10 MIC); the concentration of luteolin used in Nanfeng tangerine was 0 g/L(CK) and 3 g/L(1 MIC). 10 uL of Penicillium italicum spore suspension was injected into the wound. After the wound was dried naturally, luteolin with different concentrations was reinjected into the wound. The inoculated fruit was placed in a sterilized fresh-keeping box and cultured at 25°C.
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The disease incidence of fruit was observed every day, and the number of diseased fruits and the lesion diameter were measured. The experiment was repeated three times.
As shown in Fig. 3, the ‘Newhall’ navel orange (Fig. 3B) and Nanfeng tangerine (Fig. 3C) were inoculated with Penicillium italicum, and both of them started to get diseased on the 2nd day. Luteolin can significantly delay the onset of citrus fruits. After inoculation for 3 d, the diameter of the lesion of ‘Newhall’ navel orange fruit reached 1.77 cm, and the diameter of the lesion of fruit under luteolin treatment reached 1.54 cm, which exhibit significant differnce than that of the control group. After 3 days of inoculation of Nanfeng tangerine fruit, the lesion diameter in control group was 2.70 cm, and that in luteolin treated group was 2.02 cm, with significant difference. The results indicated that luteolin treatment could delay the pathogenesis of the blue mold disease in citrus fruit significantly.
Embodiment 4
The application of luteolin in postharvest citrus quality maintenance and decay delay, and the application method is as follows: 1. 1800 mature fruits of citrus variety 'Nanfeng tangerine' (Nanfeng county, Jiangxi) were selected and divided into two groups, namely control group (CK) and experimental group (3 g/L luteolin). There were 900 Nanfeng tangerines in each group, of which 400 fruits were used to count the decay rate, 30 fruits were used to count the weight loss rate, and the remaining fruits were used to measure CCI and TSS, determination were carried out once every 10 days, with 30 fruits each time. 2. Preparation of luteolin solution. Luteolin was mixed with ethanol and water with ethanol:water of 1:10 (volume ratio) to prepare a solution with a concentration of 3 g/L.
Nanfeng tangerine fruit was soaked in the luteolin solution for 5 min, the treated solution without luteolin is the control group. After air drying, it was stored at normal temperature (20°C, relative humidity 80-90%) and sampled every 10 days. 3. Decay rate (%)=number of decayed fruits/total number of fruitsx 100, and the final result was represented as the average of four replicates.
The effects of Luteolin on postharvest storage quality and decay of citrus are shown in
Table 3 and Fig. 4. The fruit decay rate and weight loss rate of luteolin treated group were significantly lower than those of the control group at the same period, the fruit color index (CCI) of luteolin treated group was lower than that of the control group, while the content of soluble solids (TSS) was significantly higher than that of the control group, which indicated
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Table 3 Effects of Luteolin on postharvest storage quality and decay of citrus
Tre Decay rate Weight loss rate TSS CCI am —— ent CK Luteolin CK Luteolin CK Luteolin CK Luteolin 0.00+£0.00 0.00£0.00 0.00£0.0 0.00£0.00 15.60£0.1 15.60+£0.1 2.90+0.0 2.90+0.09 0 d a a 02 a 02 02 oa a 2.00+£0.41 0.00£0.00 2.08+0.1 1.35+0.13 15.93£0.1 16.30£0.2 5.14+0.1 449+016 d a c 02 b 42 ob 28 a 3.00£091 0.500.229 436+£0.1 1.80+0.13 143801 164801 6.11+0.1 5.72+0.12 d a b ga d 28 1° 12 b 4.25+1.18 0.75+0.48 4.83+0.1 2.48+0.19 14.76+0.1 16.66+0.1 73401 6.12+0.15 d a b 62 c 5a 4b 5a b 8.00+£1.78 1.750.63 5.360.1 3.08+0.21 15.53£0.1 16.73+0.1 7.10£0.0 6.86+0.12 d a b 7a b 12 42 ga b 950+1.94 2.25+0.85 5.860 1 4.030.227 154301 172600 74101 7.12+0.14 d a b 7a b 3a ga 3a b 60 19.50+18 475131 631+£0.1 4.574029 15.240 1 16.43+£0.1 7.73£0.1 7.66+0.12 d sa d ga b 28 28 12 b
The above-mentioned embodiments only describe the preferred mode of the invention, and do not limit the scope of the invention. Under the premise of not departing from the design spirit of the invention, various modifications and improvements made by ordinary technicians in the field to the technical scheme of the invention shall fall within the protection scope determined by the claims of the invention.
Claims (10)
1. A preservative for inhibiting postharvest diseases of citrus, wherein, preservative comprises luteolin.
2. The preservative as claimed in claim 1, wherein, the diseases include blue mold and/or sour rot.
3. The preservative as claimed in claim 2, wherein, the blue mold refers to the blue mold caused by Penicillium italicum.
4. The preservative as claimed in claim 2, wherein, the sour rot refers to the sour rot caused by Geotrichum citri-aurantii.
5. The preservative as claimed in claim 1, wherein, the concentration of luteolin solution is not less than 3 g/L.
6. A method for inhibiting postharvest diseases of citrus, wherein, citrus fruits are soaked in the preservative as claimed in claim 1 for 3-7 min, and air-dried thereafter.
7. The application of the preservative as claimed in claim 1 in inhibiting the mycelial growth of Penicillium italicum.
8. The application of the preservative as claimed in claim 1 in inhibiting the mycelial growth of Geotrichum citri-aurantii.
9. The application of the preservative as claimed in claim 1 in delaying postharvest decay of citrus.
10. The application of the preservative as claimed in claim 1 in delaying the postharvest weight loss of citrus.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| LU504365A LU504365B1 (en) | 2023-05-31 | 2023-05-31 | Application of luteolin in inhibiting the postharvest diseases of citrus fruit |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| LU504365A LU504365B1 (en) | 2023-05-31 | 2023-05-31 | Application of luteolin in inhibiting the postharvest diseases of citrus fruit |
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