LU504250B1 - Method for preparing composite organic carbon source of sulfate-reducing bacteria - Google Patents
Method for preparing composite organic carbon source of sulfate-reducing bacteria Download PDFInfo
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- LU504250B1 LU504250B1 LU504250A LU504250A LU504250B1 LU 504250 B1 LU504250 B1 LU 504250B1 LU 504250 A LU504250 A LU 504250A LU 504250 A LU504250 A LU 504250A LU 504250 B1 LU504250 B1 LU 504250B1
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- carbon source
- sulfate
- fermentation
- reducing bacteria
- preparing
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- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 title claims abstract description 51
- 229910052799 carbon Inorganic materials 0.000 title claims abstract description 51
- 241000894006 Bacteria Species 0.000 title claims abstract description 33
- 238000000034 method Methods 0.000 title claims abstract description 28
- 239000002131 composite material Substances 0.000 title claims description 14
- 238000000855 fermentation Methods 0.000 claims abstract description 66
- 230000004151 fermentation Effects 0.000 claims abstract description 48
- 239000003673 groundwater Substances 0.000 claims abstract description 40
- 239000002253 acid Substances 0.000 claims abstract description 29
- 238000002386 leaching Methods 0.000 claims abstract description 29
- 238000011065 in-situ storage Methods 0.000 claims abstract description 28
- 229910052770 Uranium Inorganic materials 0.000 claims abstract description 27
- 230000002378 acidificating effect Effects 0.000 claims abstract description 27
- JFALSRSLKYAFGM-UHFFFAOYSA-N uranium(0) Chemical compound [U] JFALSRSLKYAFGM-UHFFFAOYSA-N 0.000 claims abstract description 27
- 238000005065 mining Methods 0.000 claims abstract description 25
- 239000007788 liquid Substances 0.000 claims abstract description 20
- 239000002699 waste material Substances 0.000 claims description 19
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Natural products CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 claims description 13
- 238000002156 mixing Methods 0.000 claims description 8
- 239000007787 solid Substances 0.000 claims description 8
- 239000006228 supernatant Substances 0.000 claims description 6
- 125000000218 acetic acid group Chemical group C(C)(=O)* 0.000 claims description 5
- 238000002360 preparation method Methods 0.000 claims description 5
- 241000287828 Gallus gallus Species 0.000 claims description 2
- 241000209140 Triticum Species 0.000 claims description 2
- 235000021307 Triticum Nutrition 0.000 claims description 2
- 229910052757 nitrogen Inorganic materials 0.000 claims description 2
- 229910052698 phosphorus Inorganic materials 0.000 claims description 2
- 239000010902 straw Substances 0.000 claims description 2
- 239000002023 wood Substances 0.000 claims description 2
- 241000196324 Embryophyta Species 0.000 claims 4
- 239000010828 animal waste Substances 0.000 claims 4
- 239000010908 plant waste Substances 0.000 claims 1
- 238000005067 remediation Methods 0.000 abstract description 4
- 230000007613 environmental effect Effects 0.000 abstract description 2
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 3
- 229910001385 heavy metal Inorganic materials 0.000 description 3
- 239000002994 raw material Substances 0.000 description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 3
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 2
- QAOWNCQODCNURD-UHFFFAOYSA-N Sulfuric acid Chemical compound OS(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 description 2
- 150000001720 carbohydrates Chemical class 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 239000003344 environmental pollutant Substances 0.000 description 2
- 150000002500 ions Chemical class 0.000 description 2
- 239000011572 manganese Substances 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 230000004060 metabolic process Effects 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 231100000719 pollutant Toxicity 0.000 description 2
- 238000005070 sampling Methods 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 239000011573 trace mineral Substances 0.000 description 2
- 235000013619 trace mineral Nutrition 0.000 description 2
- BJEPYKJPYRNKOW-REOHCLBHSA-N (S)-malic acid Chemical compound OC(=O)[C@@H](O)CC(O)=O BJEPYKJPYRNKOW-REOHCLBHSA-N 0.000 description 1
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 1
- KRKNYBCHXYNGOX-UHFFFAOYSA-K Citrate Chemical compound [O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O KRKNYBCHXYNGOX-UHFFFAOYSA-K 0.000 description 1
- 235000008733 Citrus aurantifolia Nutrition 0.000 description 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
- JVTAAEKCZFNVCJ-UHFFFAOYSA-M Lactate Chemical compound CC(O)C([O-])=O JVTAAEKCZFNVCJ-UHFFFAOYSA-M 0.000 description 1
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 1
- XBDQKXXYIPTUBI-UHFFFAOYSA-M Propionate Chemical compound CCC([O-])=O XBDQKXXYIPTUBI-UHFFFAOYSA-M 0.000 description 1
- QAOWNCQODCNURD-UHFFFAOYSA-L Sulfate Chemical compound [O-]S([O-])(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-L 0.000 description 1
- 235000011941 Tilia x europaea Nutrition 0.000 description 1
- BJEPYKJPYRNKOW-UHFFFAOYSA-N alpha-hydroxysuccinic acid Natural products OC(=O)C(O)CC(O)=O BJEPYKJPYRNKOW-UHFFFAOYSA-N 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 1
- 229910052791 calcium Inorganic materials 0.000 description 1
- 239000011575 calcium Substances 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 238000004070 electrodeposition Methods 0.000 description 1
- 238000003912 environmental pollution Methods 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 239000008103 glucose Substances 0.000 description 1
- 238000011835 investigation Methods 0.000 description 1
- 229910052742 iron Inorganic materials 0.000 description 1
- 239000004571 lime Substances 0.000 description 1
- 239000001630 malic acid Substances 0.000 description 1
- 235000011090 malic acid Nutrition 0.000 description 1
- WPBNNNQJVZRUHP-UHFFFAOYSA-L manganese(2+);methyl n-[[2-(methoxycarbonylcarbamothioylamino)phenyl]carbamothioyl]carbamate;n-[2-(sulfidocarbothioylamino)ethyl]carbamodithioate Chemical compound [Mn+2].[S-]C(=S)NCCNC([S-])=S.COC(=O)NC(=S)NC1=CC=CC=C1NC(=S)NC(=O)OC WPBNNNQJVZRUHP-UHFFFAOYSA-L 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 238000013048 microbiological method Methods 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 150000007524 organic acids Chemical class 0.000 description 1
- 239000005416 organic matter Substances 0.000 description 1
- 239000010815 organic waste Substances 0.000 description 1
- 239000011574 phosphorus Substances 0.000 description 1
- 239000002910 solid waste Substances 0.000 description 1
- 231100000331 toxic Toxicity 0.000 description 1
- 230000002588 toxic effect Effects 0.000 description 1
- 238000004073 vulcanization Methods 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
- C02F3/345—Biological treatment of water, waste water, or sewage characterised by the microorganisms used for biological oxidation or reduction of sulfur compounds
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/006—Radioactive compounds
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/10—Inorganic compounds
- C02F2101/101—Sulfur compounds
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2103/00—Nature of the water, waste water, sewage or sludge to be treated
- C02F2103/06—Contaminated groundwater or leachate
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2103/00—Nature of the water, waste water, sewage or sludge to be treated
- C02F2103/10—Nature of the water, waste water, sewage or sludge to be treated from quarries or from mining activities
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2305/00—Use of specific compounds during water treatment
- C02F2305/06—Nutrients for stimulating the growth of microorganisms
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Microbiology (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Biodiversity & Conservation Biology (AREA)
- Hydrology & Water Resources (AREA)
- Health & Medical Sciences (AREA)
- Environmental & Geological Engineering (AREA)
- Water Supply & Treatment (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Purification Treatments By Anaerobic Or Anaerobic And Aerobic Bacteria Or Animals (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The present invention belongs to the technical field of environmental protection and remediation of groundwater by acid in-situ leaching uranium mining, and in particular relates to a method for preparing a mixed organic carbon source suitable for sulfate-reducing bacteria in a biochemical remediation process of groundwater by acid in-situ leaching uranium mining. The method comprises the following steps: step 1, preparing a fermentation feed liquid; step 2, performing acid fermentation; step 3, adding a carbon source; and step 4, treating acidic groundwater. The method can provide a cheap and high-quality carbon source for sulfate-reducing bacteria, and greatly reduces the cost of treating the groundwater by acid in-situ leaching uranium mining.
Description
BL-5685
LU504250
METHOD FOR PREPARING COMPOSITE ORGANIC CARBON
SOURCE OF SULFATE-REDUCING BACTERIA
The present invention belongs to the technical field of environmental protection and remediation of groundwater by acid in-situ leaching uranium mining, and in particular relates to a method for preparing a mixed organic carbon source suitable for sulfate-reducing bacteria in a biochemical remediation process of groundwater by acid in-situ leaching uranium mining.
Acid in-situ leaching uranium mining is one of the main technologies for uranium mining in China. The used leaching agent sulfuric acid has strong chemical activity, and other toxic and harmful elements in an ore-bearing bed are leached while the uranium is leached, consequently, the components of groundwater after final mining in an in-situ leaching stope are relatively complex. Through investigation of groundwater in stopes of acid in-situ leaching uranium mines that have ceased production, the groundwater contains various heavy metal ions and also has high sulfate radicals, so the groundwater needs to be remediated after the final mining in the acid in-situ leaching stope.
If methods such as a lime method, a vulcanization method, and an extraction electrodeposition method are used to treat the groundwater by acid in-situ leaching uranium mining, a large amount of solid waste is generated, and secondary pollution is easily caused. When the acidic groundwater is treated by a microbiological method, the cost is low, the practicability is high, no secondary pollution is caused, and the method is the most advanced technology at present. This method uses the metabolism of sulfate-reducing bacteria (SRB) to reduce SO4> to S”, so as to remove sulfate and heavy metal ions and improve the pH value.
Because the organic matter content in the groundwater by acid in-situ leaching uranium mining is low, a sufficient carbon source needs to be provided when the sulfate-reducing bacteria are used to treat the groundwater by acid in-situ leaching uranium mining. If lactate, propionate, malic acid, ethanol, glucose, citrate, and the like are used as carbon sources, these carbon sources are relatively expensive, and the cost of treating the groundwater by acid in-situ leaching uranium mining will be higher. Therefore, it is necessary to find a cheap composite carbon source as a carbon source of the sulfate-reducing bacteria, and the composite carbon source can not only meet the needs of sulfate-reducing bacteria growth and metabolism, but also will not increase the COD value of the treated groundwater by acid in-situ leaching uranium mining.
An objective of the present invention is to provide a sufficient and cheap carbon source for sulfate-reducing bacteria when treating groundwater by acid in-situ leaching uranium mining, and a method for preparing a mixed organic carbon source 1
BL-5685 is provided. LU504250
In order to achieve the above objective, the present invention discloses a method for preparing a mixed organic carbon source which can be fully utilized by sulfate-reducing bacteria, which comprises the following steps: mixing corncob (with a granularity of 1-3 cm) and cow waste in a dry weight ratio (8-10):1, after the mixing is completed, inoculating fermentation inocula, and keeping a concentration of the total solid in the fermentation feed liquid at 6%-10%; placing the fermentation feed liquid in an acidic anaerobic fermentation tank for fermentation, wherein the dominant flora in the acidic anaerobic fermentation tank is acetic acid flora, and controlling a fermentation temperature to be 25-35 °C and fermentation time to be 12-18 d; and taking out a supernatant in the acidic anaerobic fermentation tank that is generated under the conditions as a carbon source of sulfate-reducing bacteria for treating groundwater by acid in-situ leaching uranium mining, wherein an addition amount is controlled by a ratio of the mixed COD to SO4* with a control range of 1.2-2.0.
The organic carbon source prepared by the method is used as a carbon source of sulfate-reducing bacteria to treat groundwater by acid in-situ leaching uranium mining, which has the following two advantages. 1. A high-quality carbon source of sulfate-reducing bacteria without increasing
COD of the effluent
The organic carbon source prepared by the method not only contains a large amount of saccharides, micromolecular organic acid, and carbohydrate, but also contains nitrogen, phosphorus, calcium, iron and other trace elements, so that the organic carbon source not only can provide stable energy substances and electron donors for the sulfate-reducing bacteria, but also can provide the trace elements which have an important influence on the growth of the microorganisms, and therefore is a high-quality carbon source of the sulfate-reducing bacteria. Meanwhile, organic matters in the organic carbon source can be fully utilized by sulfate-reducing bacteria, and when the organic carbon source is used to treat groundwater by acid in-situ leaching uranium mining, the COD of the effluent cannot be increased as long as the proper C/S ratio is controlled. 2. Reduction of the cost of treating groundwater by acid in-situ leaching uranium mining
The raw materials for preparing the organic carbon source are corncob and cow waste, and have the characteristics of convenient material acquisition, sufficient source, and low cost, so when the corncob and the cow waste are used to prepare the organic carbon source, in one aspect, the corncob and the cow waste are recycled, and the environmental pollution can be reduced, and in another aspect, a cheap and high-quality carbon source can be provided for sulfate-reducing bacteria, and the cost for treating the groundwater by acid in-situ leaching uranium mining is greatly reduced.
The technical solutions for achieving the objective of the present invention comprise the following steps: 2
BL-5685 step 1. preparation of a fermentation feed liquid: mixing corncob (with a 504250 granularity of 1-3 cm) and cow waste in a dry weight ratio (8-10):1, after the mixing is completed, inoculating fermentation inocula, and keeping a concentration of the total solid in the fermentation feed liquid at 6%-10%; step 2. acid fermentation: placing the fermentation feed liquid in an acidic anaerobic fermentation tank for fermentation, wherein the dominant flora in the acidic anaerobic fermentation tank is acetic acid flora, and controlling a fermentation temperature to be 25-35 °C and fermentation time to be 12-18 d; and step 3. addition of a carbon source: taking out a supernatant in the acidic anaerobic fermentation tank that is generated under the conditions as a carbon source of sulfate-reducing bacteria for treating groundwater by acid in-situ leaching uranium mining, wherein an addition amount is controlled by a ratio of the mixed COD to
SO4* with a control range of 1.2-2.0.
The fermentation raw material in the step 1 can be one of organic waste materials such as wheat straw, wood chips, corncob, cow waste, and chicken waste or the combination of different waste materials, and the combination of the corncob and the cow waste is preferably selected as the fermentation raw material in the present invention.
In the step 1, the corncob (with a granularity of 1-3 cm) and cow waste are mixed in a dry weight ratio (8-10):1, and then the mixture is inoculated into fermentation inocula after the mixing is completed, wherein the corncob and the cow waste are mixed preferably in a dry weight ratio of 10:1 in the present invention.
In the step 1, a concentration of the total solid in the fermentation feed liquid is 6%-10%, and the concentration of the total solid in the fermentation feed liquid is preferably 8.5% in the present invention.
In the step 2, the dominant flora in the acidic anaerobic fermentation tank can be different facultative anaerobic fermentation flora, and the dominant flora is preferably acetic acid fermentation flora in the present invention.
In the step 2, the fermentation temperature is controlled to be 25-35 °C, and the fermentation temperature is preferably 30 °C in the present invention. 3
BL-5685
So So LU504250
In the step 2, the fermentation time is 12-18 d, and the fermentation time is preferably 15 d in the present invention.
In the step 3, an addition amount of the carbon source is controlled by a ratio of the mixed COD to SO1” with a control range of 1.2-2.0, and the addition amount of the carbon source is controlled preferably by a ratio of 1.5 in the present invention.
The present invention has been used in the treatment test of the groundwater acid in-situ leaching uranium mine No. 381 in Yunnan province. The mixed organic carbon source prepared by the preparation method becomes a cheap and high-quality carbon source of sulfate-reducing bacteria in the treatment test of the groundwater acid in-situ leaching uranium mine No. 381 in Yunnan province.
The present invention is further described in detail below with reference to the following examples.
Example 1
In this test, two 30 m sampling holes were drilled in the mine site No. 381, and the ore bed water extracted from the two sampling holes was mixed in 1:1 to serve as the acidic groundwater studied and treated in the test. The composition and concentration of main pollutants of the acidic groundwater were analyzed, and the analysis result is shown in Table 1.
Table 1. Main pollutants and concentration of acidic groundwater studied in the test ps/(mg - L”)
Item | pH | U |NOy | SO | Cr | Cu | Cd | Pb | Mn | TDS
Final mining | 2.68 | 23.5 | 2.0 | 2780.0 | 0.01 | 0.05 13.9 | 14.1 | 4589.0 value
It can be seen from Table 1, the groundwater of the in-situ leaching stope No. 381 has high pH, uranium, sulfate radicals, and heavy metals of lead and manganese, and the concentration of the groundwater needs to be reduced through treatment so as to meet the Class IV water quality standard for groundwater quality.
The present invention has the following use effects.
In the test, sulfate radicals in the acidic groundwater of the in-situ leaching stope
No. 381 are treated by sulfate-reducing bacteria, and the mixed organic carbon source prepared by the preparation method of the present invention is used as a carbon source, and the specific steps are as follows: step 1. preparation of a fermentation feed liquid: mixing corncob (with a granularity of 1-3 cm) and cow waste in a dry weight ratio of 10:1, after the mixing 1s 4
BL-5685 . . Lo . . LU504250 completed, inoculating fermentation inocula, and keeping a concentration of the total solid in the fermentation feed liquid at 8.5%; step 2. acid fermentation: placing the fermentation feed liquid in an acidic anaerobic fermentation tank for fermentation, wherein the dominant flora in the acidic anaerobic fermentation tank is acetic acid flora, and controlling a fermentation temperature to be 30 °C and fermentation time to be 15 d; step 3. addition of a carbon source: taking out a supernatant in the acidic anaerobic fermentation tank that is generated under the conditions as a carbon source of sulfate-reducing bacteria for treating groundwater by acid in-situ leaching uranium mining, wherein an addition amount is controlled by a ratio of the COD to SO4* in the mixed treatment liquid of 1.5; and step 4. treatment of acidic groundwater: treating the treatment liquid added with the mixed organic carbon source by an EGSB bioreactor under the conditions of 20 °C and pH = 4, keeping the liquid inlet speed at 15 mL-h!, and operating for 30 days to ensure that all indexes of the effluent quality of the treated acidic groundwater meet the Class IV water quality standard for groundwater quality (see Table 2).
Table 2. Effluent quality of acidic groundwater after treatment pa/(mg - L™)
Item | pH | U [NOs | SO," | Cr | Cu | Cd | Pb | Mn | COD
Effluent quality
Claims (10)
1. A method for preparing a composite organic carbon source of sulfate-reducing bacteria, comprising the following steps: step 1. preparation of a fermentation feed liquid: mixing a plant and animal waste, and keeping a mass concentration of a solid in the fermentation feed liquid at 6%-10%; step 2. acid fermentation: placing the fermentation feed liquid in an acidic anaerobic fermentation tank for fermentation, wherein the dominant flora in the acidic anaerobic fermentation tank 1s facultative anaerobic fermentation flora; controlling a fermentation temperature to be 25-35 °C and fermentation time to be 12-18 d: step 3. addition of a carbon source: taking out a supernatant in the acidic anaerobic fermentation tank that is generated under the conditions as a carbon source of sulfate-reducing bacteria for treating groundwater by acid in-situ leaching uranium mining, wherein an addition amount is controlled by a ratio of the mixed COD to SO4” with a control range of
1.2-2.0; step 4. treatment of acidic groundwater: treating the carbon source-added groundwater by acid in-situ leaching uranium mining by an EGSB bioreactor under conditions that the temperature 1s 20 °C and the pH is 4, wherein liquid inlet is kept at a speed of 15 mL-h-1.
2. The method for preparing the composite organic carbon source of sulfate-reducing bacteria according to claim 1, wherein in the step 1, the plant is one or more of wheat straws, wood chips and corncobs which are easy to ferment and decompose into small molecular organic matters, and the animal waste is one or more of cow waste and chicken waste containing N, P, and K elements.
3. The method for preparing the composite organic carbon source of sulfate-reducing bacteria according to claim 2, wherein in the step 1, the plant is a 6
BL-5685 corncob, and the animal waste is cow waste. HU504250
4. The method for preparing the composite organic carbon source of sulfate-reducing bacteria according to claim 3, wherein in the step 1, the corncob has a granularity of 1-3 cm, and the corncob and the cow waste are mixed in a dry weight ratio of (8-10):1.
5. The method for preparing the composite organic carbon source of sulfate-reducing bacteria according to claim 4, wherein in the step 1, the corncob and the cow waste are mixed in a dry weight ratio of 10:1.
6. The method for preparing the composite organic carbon source of sulfate-reducing bacteria according to claim 1, wherein in the step 1, the mass concentration of the total solid in the fermentation feed liquid is 8.5%.
7. The method for preparing the composite organic carbon source of sulfate-reducing bacteria according to claim 1, wherein in the step 2, the dominant flora in the acidic anaerobic fermentation tank is acetic acid flora.
8. The method for preparing the composite organic carbon source of sulfate-reducing bacteria according to claim 1, wherein in the step 2, the fermentation temperature and the fermentation time are controlled to be 30 °C and 15 d.
9. The method for preparing the composite organic carbon source of sulfate-reducing bacteria according to claim 1, wherein in the step 3, the addition amount of the supernatant in the acidic anaerobic fermentation tank is controlled by a ratio of the mixed COD to SO4” with a control value of 1.5.
10. The method for preparing the composite organic carbon source of sulfate-reducing bacteria according to claim 1, wherein in the step 1, the plant is a corncob, and the animal waste is cow waste; the corncob has a granularity of 1-3 cm, the corncob and the cow waste are mixed in a dry weight ratio of 10:1, and the mass concentration of the total solid in the fermentation feed liquid is 8.5%; in the step 2, the dominant flora in the acidic anaerobic fermentation tank is acetic acid flora, and the fermentation temperature and the fermentation time are controlled to be 30 °C and 15 d; and 7
BL-5685 in the step 3, the addition amount of the supernatant in the acidic anaerobic 7504250 fermentation tank is controlled by a ratio of the mixed COD to SO4* with a control value of 1.5. 8
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| LU504250A LU504250B1 (en) | 2023-05-17 | 2023-05-17 | Method for preparing composite organic carbon source of sulfate-reducing bacteria |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| LU504250A LU504250B1 (en) | 2023-05-17 | 2023-05-17 | Method for preparing composite organic carbon source of sulfate-reducing bacteria |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| LU504250B1 true LU504250B1 (en) | 2023-11-30 |
Family
ID=88925426
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| LU504250A LU504250B1 (en) | 2023-05-17 | 2023-05-17 | Method for preparing composite organic carbon source of sulfate-reducing bacteria |
Country Status (1)
| Country | Link |
|---|---|
| LU (1) | LU504250B1 (en) |
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2023
- 2023-05-17 LU LU504250A patent/LU504250B1/en active IP Right Grant
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