LU504223B1 - Ultra-thin and highly hydrophobic multi-characteristic biofilm co-cultured by diatoms and bacterial cellulose and preparation method thereof - Google Patents
Ultra-thin and highly hydrophobic multi-characteristic biofilm co-cultured by diatoms and bacterial cellulose and preparation method thereof Download PDFInfo
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- 150000001732 carboxylic acid derivatives Chemical class 0.000 claims abstract description 49
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- 238000005406 washing Methods 0.000 claims description 5
- VMHLLURERBWHNL-UHFFFAOYSA-M Sodium acetate Chemical compound [Na+].CC([O-])=O VMHLLURERBWHNL-UHFFFAOYSA-M 0.000 claims description 4
- 238000007605 air drying Methods 0.000 claims description 4
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- 235000017281 sodium acetate Nutrition 0.000 claims description 4
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- 239000012528 membrane Substances 0.000 description 6
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- NIPNSKYNPDTRPC-UHFFFAOYSA-N N-[2-oxo-2-(2,4,6,7-tetrahydrotriazolo[4,5-c]pyridin-5-yl)ethyl]-2-[[3-(trifluoromethoxy)phenyl]methylamino]pyrimidine-5-carboxamide Chemical compound O=C(CNC(=O)C=1C=NC(=NC=1)NCC1=CC(=CC=C1)OC(F)(F)F)N1CC2=C(CC1)NN=N2 NIPNSKYNPDTRPC-UHFFFAOYSA-N 0.000 description 1
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- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/04—Polysaccharides, i.e. compounds containing more than five saccharide radicals attached to each other by glycosidic bonds
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- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/12—Unicellular algae; Culture media therefor
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P39/00—Processes involving microorganisms of different genera in the same process, simultaneously
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Abstract
The invention relates to the technical field of biomaterials, to an ultra-thin and highly hydrophobic multi-characteristic biofilm co-cultured by diatoms, bacterial cellulose and a preparation method thereof, comprising: (1) mixing diatoms and organic carboxylic acid glucose solution for reaction, centrifuging to remove supernatant, and resuspending for precipitation to obtain organic carboxylic acid glucose diatom solution; (2) adding the organic carboxylic acid glucose diatom solution into the mixed culture solution, then adding the bacterial cellulose culture solution, performing vibration culture first, and then performing anaerobic fermentation, and taking out and drying the milky translucent film to obtain the biofilm; the mixed culture solution comprises: 250mL of coconut juice, 30-50g of sucrose, 15-30g of apple vinegar, 10-15g of agar and 300-500mL of water, and the pH is adjusted to 6.0-6.5. According to the invention, an ultra-thin, ultra-light skin-friendly biomaterial with high hydrophobicity is prepared by mixing composite diatoms and bacterial cellulose.
Description
DESCRIPTION LU504223
ULTRA-THIN AND HIGHLY HYDROPHOBIC MULTI-CHARACTERISTIC BIOFILM
CO-CULTURED BY DIATOMS AND BACTERIAL CELLULOSE AND PREPARATION
METHOD THEREOF
The invention relates to the technical field of biomaterials, in particular to an ultra-thin and highly hydrophobic multi-characteristic biofilm co-cultured by diatoms and bacterial cellulose and a preparation method thereof.
As a microbial algae, diatom appeared about 250 million years ago, even earlier than human beings. Diatoms have evolved for so long and have not been eliminated by nature, which is attributed to their spongy porous cell structure and the outer wall of cells made of silicon dioxide, which enables them to carry out photosynthetic conversion efficiently. The application of diatomite has been guided by the murals of Dunhuang
Mogao Grottoes for thousands of years.
Here, according to the current use of diatoms, it can be divided into two categories: the first category is the direct application of diatom formed after the death of diatoms and the fossilization of billions of years. That is, diatomite is widely used in industrial building materials, beauty, food and other fields through purification and compound preparation.
The second category is to find the progress of diatom to materials through the cultivation and observation of living diatoms or DNA modification, and to find more possibilities. For example, the completed diatom body mechanism can carry a large number of drugs, and can realize targeted drug transport and slow release after being magnetized; or living diatoms contain lipids, which is also an excellent choice for bio-petroleum refining today;
For another example, wearable micro-current human treatment equipment ls/504223 compounded by the conductivity of pure diatom organisms. Compared with these two types, the advantages of the first type are easy to obtain, low use cost and easy operation, and its use focuses on the characteristics of diatomite materials themselves.
Disadvantages are: the depth of research and development is limited, because it is the product of petrochemical industry. During the hundreds of millions of years of transformation, the particularity of diatom as a magical creature has been erased, and its unique porous structure has been seriously damaged. In addition, the complex growth environment during the hundreds of millions of years of petrochemical industry has made diatom's own composition complex, filtration complex, chemical purification pollution serious, and the characteristics of diatom itself have also been greatly reduced. And diatomite itself is not an inexhaustible natural resource. The second category belongs to the search for infinite possibilities by returning diatom to the nature of biological attributes, and by studying diatom and the characteristics of diatom special institutions.
Diatoms are easy to catch, easy to breed, low in cost, and the reproduction speed can be doubled in 4 hours, which is really inexhaustible and a new era for human beings to re-examine and use diatoms in the new era. However, at present, there are few reports on the preparation of biomaterials from living diatoms.
SUMMARY LU504223
The purpose of the present invention is to provide an ultra-thin and highly hydrophobic multi-characteristic biofilm co-cultured by diatoms and bacterial cellulose and a preparation method thereof, so as to solve the problems existing in the prior art. By using diatoms with super-strong adsorption capacity and super-high biological compatibility to carry organic carboxylic acid glucose, and combining the carrying and recombination capacity of bacterial cellulose, diatoms carrying organic carboxylic acid glucose grow in the body structure of bacterial cellulose, so that bacterial cellulose without hydrophobic ability originally has a certain hydrophobic ability, and at the same time has a tough, light and skin-friendly texture of bacterial cellulose.
In order to achieve the above objectives, the present invention provides the following scheme.
The invention provides a method for preparing an ultra-thin and highly hydrophobic multi-characteristic biofilm co-cultured by diatoms and bacterial cellulose, comprising: (1) mixing diatoms and organic carboxylic acid glucose solution for reaction, centrifuging to remove supernatant, and resuspending for precipitation to obtain organic carboxylic acid glucose diatom solution; (2) adding the organic carboxylic acid glucose diatom solution into the mixed culture solution, then adding the bacterial cellulose culture solution, performing vibration culture first, and then performing anaerobic fermentation, and taking out and drying the milky translucent film to obtain the biofilm; the mixed culture solution comprises: 250mL of coconut juice, 30-50g of sucrose, 15-30g of apple vinegar, 10-15g of agar and 300-500mL of water, and the pH is adjusted to 6.0-6.5.
Optionally, in step (1), the volume ratio of the diatoms to the organic carboxylic acid glucose solution is 1: (5-10) (mL:mL).
Optionally, in step (1), the mixing reaction conditions are: stirring at 100-110°C for 2-3 hours; centrifuge conditions are: centrifuging at 3500 rpm for 5 minutes.
Optionally, in step (2), the volume ratio of the organic carboxylic acid glucose diatom solution to the mixed culture solution is 1:(15-20) (mL:mL).
Optionally, in step (2), the inoculation amount of the bacterial cellulose cultut&/504223 solution is 4%-5% of the mixed culture solution.
Optionally, in step (2), the condition of the shaking culture is: shaking table at room temperature for 30 minutes at 180r/min.
Optionally, in step (2), the conditions of anaerobic fermentation are: anaerobic fermentation at 30-50°C for 24-36h, or performing anaerobic fermentation at 30-50°C for 24-36h, adding organic carboxylic acid glucose diatom liquid into the anaerobic fermentation system, and continuing anaerobic fermentation at 30-50°C for 12-24h; wherein the volume ratio of the organic carboxylic acid glucose diatom solution to the total amount of the anaerobic fermentation volume mixed solution is 1: 25.
Optionally, in step (2), the drying method comprises any one of the following: (1) firstly, cleaning the film with deionized water, then soaking in a hot water bath at 120°C for 2 hours and taking it out, then placing at 60-70°C for dehydration, and when the film is not completely dried, transferring to a sodium acetate solution and soaking it for minutes, then taking out and washing with deionized water, and naturally drying to obtain the biofilm; (2) quickly dehydrating the film at 60-70°C until it is completely dry, transferring to the organic carboxylic acid glucose diatom solution heated to 110°C, ultrasonically oscillating for 30 minutes, taking out and naturally air drying, and then putting in ethanol solution for 2 days to wash off surface impurities, namely the biofilm.
The invention also provides an ultra-thin and highly hydrophobic multi-characteristic biofilm co-cultured by diatoms and bacterial cellulose, prepared by the above preparation method.
The invention discloses the following technical effects:
The ultrathin and highly hydrophobic biomembrane prepared by diatoms and bacterial cellulose is characterized in that the portability and multi-biological compatibility of diatoms are connected in series with the structural characteristics of macromolecular nano-self-assembly and the humidity adjustment ability of bacterial cellulose through the investigation of biological characteristics; according to the invention, diatoms carrying organic carboxylic acid glucose reagents are mixed in the fibers of bacterial fiber materials through the growth of bacterial cellulose, and an inseparable whole is formedU504223 so that the hydrophobicity of the material is maintained for a longer period of time, the elution is difficult, and the characteristics are stable, and at the same time, the characteristics from diatoms and bacterial cellulose are inherited, that is, the characteristics of antibiosis, skin friendliness, softness and high toughness. The biomembrane prepared by the invention belongs to regenerative biomaterials and provides combinable diversity for multifunctional biological composite materials.
The biomembrane prepared by the invention is also environment-friendly.
Compared with other hydrophobic multifunctional composite materials, the invention does not use chemical bases or acid groups to dissolve cellulose, thus facilitating the drawing and shaping of mixed functional agents. In order to ensure the activity and self-film forming ability of bacterial cellulose in the growth medium, the esterification reaction of organic carboxylic acid glucose solution replaces the dependence of other hydrophobic components, so as to reduce the use of harmful chemical components.
Therefore, the biomembrane prepared by the invention is a carbon-based biomaterial, so that it can be completely degraded and recycled, which is environmentally friendly and has a good application prospect in the field of high-precision machinery and medical treatment.
A number of exemplary embodiments of the present invention will now be described in detail, and this detailed description should not be considered as a limitation of the present invention, but should be understood as a more detailed description of certain aspects, characteristics and embodiments of the present invention.
It should be understood that the terminology described in the present invention is only for describing specific embodiments and is not used to limit the present invention. In addition, for the numerical range in the present invention, it should be understood that each intermediate value between the upper limit and the lower limit of the range is also specifically disclosed. Intermediate values within any stated value or stated range, as well as each smaller range between any other stated value or intermediate values withlrtJ504223 the stated range are also included in the present invention. The upper and lower limits of these smaller ranges can be independently included or excluded from the range.
Unless otherwise specified, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention relates. Although the present invention only describes the preferred methods and materials, any methods and materials similar or equivalent to those described herein can also be used in the practice or testing of the present invention. All documents mentioned in this specification are incorporated by reference to disclose and describe methods and/or materials related to the documents. In case of conflict with any incorporated document, the contents of this specification shall prevail.
It is obvious to those skilled in the art that many improvements and changes can be made to the specific embodiments of the present invention without departing from the scope or spirit of the present invention. Other embodiments will be apparent to the skilled person from the description of the invention. The description and example of that present invention are exemplary only.
The terms "comprising", "including", "having" and "containing" used in this article are all open terms, which means including but not limited to. 1. Culture and preparation of diatoms a. get about 500g of precipitated diatom water from the freshwater river by brushing stones, and let it stand for 7 days; b. inject them into the test tubes respectively, and the water quantity shall not be higher than 2/3 of the test tubes; c, placing in a centrifuge at 2000rpm for no more than 3 minutes, otherwise the long diatom will be broken, and then removing water; d, adding 30% hydrogen peroxide, vertically placing the glass sheet in the test tube, heating to 100%, and keeping for 1 hour; e, adding 3 drops of highly concentrated acid salt, and standing for at least 30 minutes until the reaction is finished;
f, placing a centrifuge at 2000rpm for 3 minutes, then discarding the supernatarttU504223 washing with distilled water, and sterilizing with ultraviolet rays to obtain clean and hollow diatoms with intact structures. 2. Preparation of organic carboxylic acid glucose diatom solution
Prepare a clean incubator, mix the prepared diatom with the organic carboxylic acid glucose solution at a mass-volume ratio of 1: 10, heat the mixed solution to 100°C, stir for 2 hours, then let it stand at room temperature, then move it into a centrifuge, centrifuge at a rotation speed of 3,500 rpm for 5 minutes, take it out, discard the supernatant, disperse the precipitate with distilled water, let it stand, and sterilize with ultraviolet rays to obtain the organic carboxylic acid glucose diatom solution. 3. Preparing biofilm by mixed culture of organic carboxylic acid glucose diatom solution and bacterial cellulose. (1) Preparation of mixed culture solution: sterilize the culture container with high-temperature steam, add 300mL deionized water, add 250mL coconut juice, 30g sucrose, 15g apple vinegar and 10g agar, then stir the mixture, add alkali, reduce the pH to 6.0-6.5, then move it into a sterilization tank, steam sterilize at 120°C for 30min, and stand for cooling to room temperature, thus obtaining mixed culture solution. (2) Preparation of standby bacterial fiber mixed culture solution: A.hansenii (provided by Imperial College London) was inoculated into the cooled mixed culture solution according to the inoculation amount of 4%, then moved to a constant temperature incubator, and kept at 26°C for 3 days to prepare bacterial fiber mixed culture solution. (3) adding the organic carboxylic acid glucose diatom solution into the prepared mixed culture solution according to the volume ratio of 1: 20 to obtain the organic carboxylic acid glucose diatom nutrient solution; then, the bacterial fermentation broth is extracted from the bottom of the culture container for preparing the bacterial fiber mixed culture solution according to the proportion of 5% of the total amount of the organic carboxylic acid glucose diatom nutrient solution, placed in the organic carboxylic acid glucose diatom nutrient solution at room temperature, shaken on a shaker at 180rpm for minutes, and then anaerobically cultured at 30-50°C for 2 days, and then taken out when an ivory translucent film is formed, at which time the film thickness is about 0.8 mhu504223
Then wash it with deionized water for three times, soak it in a hot water bath container at 120°C for 2 hours, then take it out, then put it in an oven at 60°C to dehydrate it quickly, and remove it when the material is still soft (don't wait until it is completely dry, otherwise it will be fragile), then soak it in sodium acetate with pH of 0.5mm at room temperature for 30 minutes, then take it out, rinse it with deionized water and naturally dry it, and you can get an ultra-thin hydrophobic biofilm prepared from diatoms and bacterial cellulose.
After drying, the film thickness is about. Its thickness can be changed according to the fermentation standing time.
Compared with a single bacterial cellulose membrane, this membrane has weak self-absorption after air-drying, a certain water content, strong toughness, soft skin-friendly texture, and can keep the current state well, but it is not completely waterproof.
Example 2 1. Culture and preparation of diatoms a, about 500g of precipitated diatom water was obtained from freshwater river by stone brushing method, and it was kept for 10 days; b, respectively inject into that test tube, wherein the water amount is not higher than 2/3 of the test tube; c, placing in a centrifuge at 2000rpm for no more than 3 minutes, otherwise the long diatom will be broken, and then removing water; d, adding 30% hydrogen peroxide, vertically placing the glass sheet in the test tube, heating to 100%, and keeping for 2 hours; e, adding 4 drops of highly concentrated acid salt, and standing for at least 30 minutes until the reaction is finished; f, placing a centrifuge at 2000rpm for 3 minutes, then discarding the supernatant, washing with distilled water, and sterilizing with ultraviolet rays to obtain clean and hollow diatoms with intact structures.
2. Preparation of organic carboxylic acid glucose diatom solution LU504223
Prepare a clean incubator, mix the prepared diatom with the organic carboxylic acid glucose solution at a mass-volume ratio of 1: 10, heat the mixed solution to 110°C, stir for 3 hours, then let it stand at room temperature, then move it into a centrifuge, centrifuge at a rotation speed of 3,500 rpm for 5 minutes, take it out, discard the supernatant, disperse the precipitate with distilled water, let it stand, and sterilize with ultraviolet rays to obtain the organic carboxylic acid glucose diatom solution. 3. Preparing biofilm by mixed culture of organic carboxylic acid glucose diatom solution and bacterial cellulose. (1) Preparation of mixed culture solution: sterilize the culture container with high-temperature steam, add 400mL deionized water, add 250mL coconut juice, 40g sucrose, 20g apple vinegar and 12g agar, then stir the mixture, add alkali, reduce the pH to 6.0, then move it into a sterilization tank, steam sterilize at 120°C for 30 minutes, and let it stand until it is cooled to room temperature, thus obtaining mixed culture solution. (2) Preparation of standby bacterial fiber mixed culture solution: A.hansenii (provided by Imperial College London) was inoculated into the cooled mixed culture solution according to the inoculation amount of 5%, then moved to a constant temperature incubator and kept at 27°C for 4 days to prepare bacterial fiber mixed culture solution. (3) Adding the organic carboxylic acid glucose diatom solution into the prepared mixed culture solution according to the volume ratio of 1:15 to obtain the organic carboxylic acid glucose diatom nutrient solution; then, the bacterial fermentation broth was extracted from the bottom of the culture container for preparing bacterial fiber mixed culture solution according to the proportion of 5% of the total amount of organic carboxylic acid glucose diatom nutrient solution, and then it was placed at room temperature and shaken on a shaker at 180rpm for 30 minutes, and then it was anaerobically cultured at 40°C for 36 hours. Inject the mixed culture dish with a syringe at the bottom of the solution again, inject the previously prepared organic carboxylic acid glucose diatom solution into the mixed culture solution after anaerobic fermentation at a volume ratio of 1: 25, continue anaerobic culture at 40°C for 12 hours, and take it out when an ivory translucent film is formed. At this time, the film thickness is about 0.6mrhU504223 then it is washed with deionized water for 3 times, soaked in a hot water bath container at 120°C for 2 hours, then it is taken out, and then it is placed in an oven at 65°C for rapid dehydration. When the material is still soft, it is taken out (don't wait until it is completely dry, otherwise it will be fragile), and then it is soaked in sodium acetate with pH of 5.5 for minutes at room temperature, then it is taken out, washed with deionized water and naturally dried, so that it can be prepared from diatom and bacterial cellulose. Its thickness can be changed according to the fermentation standing time.
Compared with a single bacterial cellulose membrane, this membrane, after being air-dried, has obviously weakened self-water absorption, significantly improved hydrophobicity, extremely strong toughness and soft skin-friendly texture, which can keep the current state well, but is not completely waterproof.
Example 3 1. Culture and preparation of diatoms a, about 500g of precipitated diatom water was obtained from freshwater river by stone brushing method, and it was kept for 14 days; b, respectively inject into that test tube, wherein the water amount is not higher than 2/3 of the test tube; c, placing in a centrifuge at 2000rpm for no more than 3 minutes, otherwise the long diatom will be broken, and then removing water; d, adding 30% hydrogen peroxide, vertically placing the glass sheet in the test tube, heating to 100%, and keeping for 2 hours; e, adding 4 drops of highly concentrated acid salt, and standing for at least 30 minutes until the reaction is finished; f, placing a centrifuge at 2000rpm for 3 minutes, then discarding the supernatant, washing with distilled water, and sterilizing with ultraviolet rays to obtain clean and hollow diatoms with intact structures.
2. Preparation of organic carboxylic acid glucose diatom solution LU504223
Prepare a clean incubator, mix the prepared diatom with the organic carboxylic acid glucose solution at a mass-volume ratio of 1: 10, heat the mixed solution to 110°C, stir for 3 hours, then let it stand at room temperature, then move it into a centrifuge, centrifuge at a rotation speed of 3,500 rpm for 5 minutes, take it out, discard the supernatant, disperse the precipitate with distilled water, let it stand, and sterilize with ultraviolet rays to obtain the organic carboxylic acid glucose diatom solution. 3. Preparing biofilm by mixed culture of organic carboxylic acid glucose diatom solution and bacterial cellulose. (1) Preparing mixed culture solution: sterilizing the culture container with high-temperature steam, adding 500mL deionized water, adding 250mL coconut juice, 50g sucrose, 30g apple vinegar and 15g agar, then stirring the mixture, adding alkali, lowering the pH to 6.5, transferring it into a sterilization tank, autoclaving at 120°C for 30 minutes, and standing for cooling to room temperature, thus obtaining mixed culture solution. (2) Preparation of standby bacterial fiber mixed culture solution: A.hansenii (provided by Imperial College London) was inoculated into the cooled mixed culture solution according to the inoculation amount of 5%, then moved to a constant temperature incubator and kept at 28°C for 5 days to prepare bacterial fiber mixed culture solution. (3) adding the organic carboxylic acid glucose diatom solution into the prepared mixed culture solution according to the volume ratio of 1: 15 to obtain the organic carboxylic acid glucose diatom nutrient solution; Then, the bacterial fermentation broth is pumped into the organic carboxylic acid glucose diatom nutrient solution from the bottom of the culture container for preparing the bacterial fiber mixed culture solution according to the proportion of 5% of the total amount of the organic carboxylic acid glucose diatom nutrient solution, and is placed at room temperature, shaken on a shaker at 180rpm for minutes, and then anaerobically cultured at 50°C for 36 hours, and then a syringe is used at the bottom of the solution for the mixed culture dish again. Inject the previously prepared organic carboxylic acid glucose diatom solution into the mixed culture solution again at the volume ratio of 1: 25, then continue anaerobic culture at 50°C for 12 hourBU504223 take it out when it forms a milky translucent film with a thickness of about 0.7mm, and then put it in an oven at 60°C for rapid dehydration to dryness. Next, the previously prepared organic carboxylic acid glucose diatom solution is heated to 100°C, taken out, and the completely dried diatom bacterial cellulose biofilm is placed in it, and then placed in an ultrasonic shaker. After ultrasonic vibration at 1000w and 1100°C for 30 minutes, it is taken out and placed in a ventilated place for natural air drying at room temperature, and then placed in 75% ethanol. After standing for 2 days, the surface impurities are washed away, and finally a highly hydrophobic diatom bacterial cellulose biofilm is obtained. At this time, the film thickness is about.
Compared with the single bacterial cellulose membrane, this membrane is still light and thin after being treated, and it no longer shows obvious self-absorption ability. It has strong hydrophobicity, excellent buoyancy, strong toughness, skin-friendly and soft texture, which can keep the current state well and has good waterproof.
The performance of the highly hydrophobic diatom bacterial cellulose biofilm prepared in Example 1-3 was tested, and the detection method was as follows:
Take 20cm of examples and comparative examples prepared by the invention, and measure the air flow rate vertically passing through the given area of the sample in a certain time under the condition of specified pressure difference, and calculate the air permeability. The evaluation standard is GB/T 5453-1997.
YG (B) 812-120 fabric water permeability tester was used to measure the water resistance. According to GB/T 4744-1997, the static water pressure borne by the fabric was used to indicate the resistance of water passing through this material.
Table 1 Performance Test Results LU504223 ome Fret (are [res CE
Example 1 | Example 2 | Example 3 characterization example
Air flow permeability 245.40 277.20 289.70 230.50 (L/m?/s)
Water pressure resistance 8.33 8.46 8.50 7.33 (kPa)
The above-mentioned embodiments only describe the preferred mode of the invention, and do not limit the scope of the invention. Under the premise of not departing from the design spirit of the invention, various modifications and improvements made by ordinary technicians in the field to the technical scheme of the invention shall fall within the protection scope determined by the claims of the invention.
Claims (9)
1. A method for preparing an ultra-thin and highly hydrophobic multi-characteristic biofilm co-cultured by diatoms and bacterial cellulose, comprising: (1) mixing diatoms and organic carboxylic acid glucose solution for reaction, centrifuging to remove supernatant, and resuspending for precipitation to obtain organic carboxylic acid glucose diatom solution; (2) adding the organic carboxylic acid glucose diatom solution into the mixed culture solution, then adding the bacterial cellulose culture solution, performing shaking culture first, and then performing anaerobic fermentation, and taking out and drying the milky translucent film to obtain the biofilm; the mixed culture solution comprises: 250mL of coconut juice, 30-50g of sucrose, 15-30g of apple vinegar, 10-15g of agar and 300-500mL of water, and the Ph value is adjusted to 6.0-6.5.
2. The preparation method according to claim 1, wherein in step (1), the volume ratio of the diatoms to the organic carboxylic acid glucose solution is 1: (5-10).
3. The preparation method according to claim 1, wherein in step (1), the mixing reaction conditions are: stirring at 100-110°C for 2-3 hours; centrifuge conditions are: centrifuging at 3500 rpm for 5 minutes.
4. The preparation method according to claim 1, wherein in step (2), the volume ratio of the organic carboxylic acid glucose diatom solution to the mixed culture solution is 1:(15-20).
5. The preparation method according to claim 1, wherein in step (2), the inoculation amount of the bacterial cellulose culture solution is 4%-5% of the mixed culture solution.
6. The preparation method according to claim 1, wherein in step (2), the condition 6504223 the shaking culture is: shaking table at room temperature for 30 minutes at 180r/min.
7. The preparation method according to claim 1, wherein in step (2), the conditions of anaerobic fermentation are: performing anaerobic fermentation at 30-50°C for 24-36h, or performing anaerobic fermentation at 30-50°C for 24-36h, adding organic carboxylic acid glucose diatom solution into the anaerobic fermentation system, and continuing anaerobic fermentation at 30-50°C for 12-24h; wherein the volume ratio of the organic carboxylic acid glucose diatom solution to the total amount of the anaerobic fermentation volume mixed solution is 1: 25.
8. The preparation method according to claim 1, wherein in step (2), the drying method comprises any one of the following methods: (1) firstly, cleaning the film with deionized water, then soaking in a hot water bath at 120°C for 2 hours and taking out, then dehydrating at 60-70°C, and when the film is not completely dried, transferring to a sodium acetate solution and soaking for 30 minutes, then taking out and washing with deionized water, and naturally drying to obtain the biofilm; (2) quickly dehydrating the film at 60-70°C until it is completely dry, transferring to the organic carboxylic acid glucose diatom solution heated to 110°C, ultrasonically oscillating for 30 minutes, taking out and naturally air drying, and then putting in ethanol solution for 2 days to wash off surface impurities and obtain the biofilm.
9. An ultra-thin and highly hydrophobic multi-characteristic biofilm co-cultured by diatoms and bacterial cellulose, prepared by the preparation method according to any one of claims 1-8.
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