LU503859B1 - Agent for restoring greening of yellow leaves of fruit tree, and use method thereof - Google Patents
Agent for restoring greening of yellow leaves of fruit tree, and use method thereof Download PDFInfo
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- LU503859B1 LU503859B1 LU503859A LU503859A LU503859B1 LU 503859 B1 LU503859 B1 LU 503859B1 LU 503859 A LU503859 A LU 503859A LU 503859 A LU503859 A LU 503859A LU 503859 B1 LU503859 B1 LU 503859B1
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- Prior art keywords
- greening
- restoring
- solution
- agent
- fruit tree
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- 235000013399 edible fruits Nutrition 0.000 title claims abstract description 81
- 238000000034 method Methods 0.000 title claims abstract description 22
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 claims abstract description 131
- 239000003795 chemical substances by application Substances 0.000 claims abstract description 90
- 229910052742 iron Inorganic materials 0.000 claims abstract description 63
- HCHKCACWOHOZIP-UHFFFAOYSA-N Zinc Chemical compound [Zn] HCHKCACWOHOZIP-UHFFFAOYSA-N 0.000 claims abstract description 54
- 229910052725 zinc Inorganic materials 0.000 claims abstract description 54
- 239000011701 zinc Substances 0.000 claims abstract description 54
- ZOKXTWBITQBERF-UHFFFAOYSA-N Molybdenum Chemical compound [Mo] ZOKXTWBITQBERF-UHFFFAOYSA-N 0.000 claims abstract description 34
- 229910052750 molybdenum Inorganic materials 0.000 claims abstract description 34
- 239000011733 molybdenum Substances 0.000 claims abstract description 34
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 26
- 229920005551 calcium lignosulfonate Polymers 0.000 claims abstract description 25
- RYAGRZNBULDMBW-UHFFFAOYSA-L calcium;3-(2-hydroxy-3-methoxyphenyl)-2-[2-methoxy-4-(3-sulfonatopropyl)phenoxy]propane-1-sulfonate Chemical compound [Ca+2].COC1=CC=CC(CC(CS([O-])(=O)=O)OC=2C(=CC(CCCS([O-])(=O)=O)=CC=2)OC)=C1O RYAGRZNBULDMBW-UHFFFAOYSA-L 0.000 claims abstract description 25
- 150000005846 sugar alcohols Chemical class 0.000 claims abstract description 25
- KGBXLFKZBHKPEV-UHFFFAOYSA-N boric acid Chemical compound OB(O)O KGBXLFKZBHKPEV-UHFFFAOYSA-N 0.000 claims abstract description 18
- 239000004327 boric acid Substances 0.000 claims abstract description 18
- 229920001661 Chitosan Polymers 0.000 claims description 57
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 claims description 36
- 239000006185 dispersion Substances 0.000 claims description 36
- KFZMGEQAYNKOFK-UHFFFAOYSA-N Isopropanol Chemical compound CC(C)O KFZMGEQAYNKOFK-UHFFFAOYSA-N 0.000 claims description 16
- 238000006243 chemical reaction Methods 0.000 claims description 16
- 239000002994 raw material Substances 0.000 claims description 16
- FOCAUTSVDIKZOP-UHFFFAOYSA-N chloroacetic acid Chemical compound OC(=O)CCl FOCAUTSVDIKZOP-UHFFFAOYSA-N 0.000 claims description 15
- 229940106681 chloroacetic acid Drugs 0.000 claims description 15
- WTDHULULXKLSOZ-UHFFFAOYSA-N Hydroxylamine hydrochloride Chemical compound Cl.ON WTDHULULXKLSOZ-UHFFFAOYSA-N 0.000 claims description 12
- 239000011790 ferrous sulphate Substances 0.000 claims description 12
- 235000003891 ferrous sulphate Nutrition 0.000 claims description 12
- NWONKYPBYAMBJT-UHFFFAOYSA-L zinc sulfate Chemical compound [Zn+2].[O-]S([O-])(=O)=O NWONKYPBYAMBJT-UHFFFAOYSA-L 0.000 claims description 9
- 239000011686 zinc sulphate Substances 0.000 claims description 9
- 235000009529 zinc sulphate Nutrition 0.000 claims description 9
- 238000003756 stirring Methods 0.000 claims description 8
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 claims description 6
- PTFCDOFLOPIGGS-UHFFFAOYSA-N Zinc dication Chemical compound [Zn+2] PTFCDOFLOPIGGS-UHFFFAOYSA-N 0.000 claims description 6
- 238000010438 heat treatment Methods 0.000 claims description 6
- BDAGIHXWWSANSR-UHFFFAOYSA-N methanoic acid Natural products OC=O BDAGIHXWWSANSR-UHFFFAOYSA-N 0.000 claims description 6
- 238000001556 precipitation Methods 0.000 claims description 6
- 238000001035 drying Methods 0.000 claims description 5
- 230000006196 deacetylation Effects 0.000 claims description 4
- 238000003381 deacetylation reaction Methods 0.000 claims description 4
- OSWFIVFLDKOXQC-UHFFFAOYSA-N 4-(3-methoxyphenyl)aniline Chemical compound COC1=CC=CC(C=2C=CC(N)=CC=2)=C1 OSWFIVFLDKOXQC-UHFFFAOYSA-N 0.000 claims description 3
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 3
- 238000010668 complexation reaction Methods 0.000 claims description 3
- 239000012153 distilled water Substances 0.000 claims description 3
- 235000019253 formic acid Nutrition 0.000 claims description 3
- 230000007935 neutral effect Effects 0.000 claims description 3
- 239000002244 precipitate Substances 0.000 claims description 3
- 238000002390 rotary evaporation Methods 0.000 claims description 3
- 238000000967 suction filtration Methods 0.000 claims description 3
- 238000005406 washing Methods 0.000 claims description 3
- 230000002378 acidificating effect Effects 0.000 claims description 2
- 230000000694 effects Effects 0.000 abstract description 11
- 230000000052 comparative effect Effects 0.000 description 11
- 238000002156 mixing Methods 0.000 description 8
- 239000007788 liquid Substances 0.000 description 7
- 201000010099 disease Diseases 0.000 description 5
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 5
- 230000002265 prevention Effects 0.000 description 5
- CWYNVVGOOAEACU-UHFFFAOYSA-N Fe2+ Chemical compound [Fe+2] CWYNVVGOOAEACU-UHFFFAOYSA-N 0.000 description 4
- 229910001448 ferrous ion Inorganic materials 0.000 description 4
- PUKLDDOGISCFCP-JSQCKWNTSA-N 21-Deoxycortisone Chemical compound C1CC2=CC(=O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@@](C(=O)C)(O)[C@@]1(C)CC2=O PUKLDDOGISCFCP-JSQCKWNTSA-N 0.000 description 3
- 241000196324 Embryophyta Species 0.000 description 3
- FCYKAQOGGFGCMD-UHFFFAOYSA-N Fulvic acid Natural products O1C2=CC(O)=C(O)C(C(O)=O)=C2C(=O)C2=C1CC(C)(O)OC2 FCYKAQOGGFGCMD-UHFFFAOYSA-N 0.000 description 3
- 206010022971 Iron Deficiencies Diseases 0.000 description 3
- 238000010521 absorption reaction Methods 0.000 description 3
- 238000005189 flocculation Methods 0.000 description 3
- 230000016615 flocculation Effects 0.000 description 3
- 239000002509 fulvic acid Substances 0.000 description 3
- 229940095100 fulvic acid Drugs 0.000 description 3
- ZOXJGFHDIHLPTG-UHFFFAOYSA-N Boron Chemical compound [B] ZOXJGFHDIHLPTG-UHFFFAOYSA-N 0.000 description 2
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 2
- 244000141359 Malus pumila Species 0.000 description 2
- 235000011430 Malus pumila Nutrition 0.000 description 2
- 235000015103 Malus silvestris Nutrition 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 2
- 229910052796 boron Inorganic materials 0.000 description 2
- 229910052791 calcium Inorganic materials 0.000 description 2
- 239000011575 calcium Substances 0.000 description 2
- 125000002057 carboxymethyl group Chemical group [H]OC(=O)C([H])([H])[*] 0.000 description 2
- 239000013522 chelant Substances 0.000 description 2
- 230000012010 growth Effects 0.000 description 2
- 238000006467 substitution reaction Methods 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 1
- VTLYFUHAOXGGBS-UHFFFAOYSA-N Fe3+ Chemical compound [Fe+3] VTLYFUHAOXGGBS-UHFFFAOYSA-N 0.000 description 1
- 241000233866 Fungi Species 0.000 description 1
- AFCARXCZXQIEQB-UHFFFAOYSA-N N-[3-oxo-3-(2,4,6,7-tetrahydrotriazolo[4,5-c]pyridin-5-yl)propyl]-2-[[3-(trifluoromethoxy)phenyl]methylamino]pyrimidine-5-carboxamide Chemical compound O=C(CCNC(=O)C=1C=NC(=NC=1)NCC1=CC(=CC=C1)OC(F)(F)F)N1CC2=C(CC1)NN=N2 AFCARXCZXQIEQB-UHFFFAOYSA-N 0.000 description 1
- 241000700605 Viruses Species 0.000 description 1
- 230000002411 adverse Effects 0.000 description 1
- 230000003042 antagnostic effect Effects 0.000 description 1
- 229940038879 chelated zinc Drugs 0.000 description 1
- 239000002738 chelating agent Substances 0.000 description 1
- -1 comprises EDTA-Fe Chemical compound 0.000 description 1
- 230000006866 deterioration Effects 0.000 description 1
- 230000004720 fertilization Effects 0.000 description 1
- 208000015181 infectious disease Diseases 0.000 description 1
- JEIPFZHSYJVQDO-UHFFFAOYSA-N iron(III) oxide Inorganic materials O=[Fe]O[Fe]=O JEIPFZHSYJVQDO-UHFFFAOYSA-N 0.000 description 1
- 229910052751 metal Inorganic materials 0.000 description 1
- 239000002184 metal Substances 0.000 description 1
- 229910021645 metal ion Inorganic materials 0.000 description 1
- 150000002739 metals Chemical class 0.000 description 1
- 230000008635 plant growth Effects 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 238000002203 pretreatment Methods 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 239000002689 soil Substances 0.000 description 1
- 239000007921 spray Substances 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 239000013589 supplement Substances 0.000 description 1
- 238000010998 test method Methods 0.000 description 1
- 230000001988 toxicity Effects 0.000 description 1
- 231100000419 toxicity Toxicity 0.000 description 1
- 238000004383 yellowing Methods 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01P—BIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
- A01P1/00—Disinfectants; Antimicrobial compounds or mixtures thereof
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01P—BIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
- A01P3/00—Fungicides
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Pest Control & Pesticides (AREA)
- Plant Pathology (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Environmental Sciences (AREA)
- Microbiology (AREA)
- Mycology (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The present disclosure discloses an agent for restoring greening of yellow leaves of a fruit tree, and a use method thereof. The agent for restoring greening of yellow leaves of a fruit tree comprises the following components in parts by weight: 40 - 60 parts by weight of an iron and zinc greening - restoring agent, 5 - 10 parts by weight of calcium lignosulfonate, 3 - 5 parts by weight of boric acid, 1 - 3 parts by weight of sugar alcohol molybdenum, and 60 - 100 parts by weight of water. The use method is as follows: when yellow leaves appear on a fruit tree, the agent for restoring greening of yellow leaves of a fruit tree is diluted with water to 50 - 100 times and sprayed onto leaf surfaces of the fruit tree. The agent for restoring greening of yellow leaves of a fruit tree of the present disclosure may rapidly restore greening of yellow leaves of a fruit tree, has a better effect of restoring greening, and may improve quality and yield of fruits.
Description
AGENT FOR RESTORING GREENING OF YELLOW LEAVES OF FRUIT TREE, AND USE
METHOD THEREOF 0503859
FIELD OF TECHNOLOGY
The present disclosure relates to the technical field of prevention and treatment of yellow leaves of a fruit tree. The present disclosure particularly discloses an agent for restoring greening of yellow leaves of a fruit tree, and a use method thereof.
Common causes of yellow leaf disease of fruit trees are: infection by fungi, bacteria or viruses, improper fertilization management, and iron deficiency of the fruit trees caused by depletion and salinization of soil. The iron deficiency yellow leaf disease of the fruit trees directly affects the growth and development of the fruit trees and the yield of fruits, also causes deterioration of taste of the fruits, even causes the death of the whole fruit trees, and seriously affects the economic value of the fruit trees.
At present, a main approach for treating the iron - deficiency yellow leaf disease of the fruit trees is to directly supplement an exogenous iron to fruits. The common exogenous iron for fruit trees is mainly divided into ferrous sulphate and organic chelated iron. Although the ferrous sulphate has a low cost, it is easily oxidized into a ferric iron which is not easily absorbed by fruit trees, such that an absorption conversion rate is low and a prevention and treatment effect is poor. The organic chelated iron mainly comprises EDTA-Fe, DTPA-Fe, EDDHA-Fe, and fulvic acid chelated iron, wherein the EDTA-Fe and DTPA-Fe have a high chelating cost. Besides, a chelating agent is mostly a chemically synthetic substance, has pollution and toxicity to the environment, is difficult to absorb and utilize in plants, and easily affects quality of fruits. The fulvic acid chelated iron is easy to absorb and has a good prevention and treatment effect, has an effect on promoting plant growth, and gradually becomes a popular product in recent years.
But the fulvic acid chelated iron has a poor flocculation resistance and is not beneficial to popularization and application. Therefore, there is a need to develop an agent for restoring greening of yellow leaves of a fruit tree, which has a good stability, is easy to be absorbed and utilized by plants, and has a good prevention and treatment effect.
For this purpose, the technical problems to be solved by the present disclosure are to provide an agent for restoring greening of yellow leaves of a fruit tree and use method thereof to solve problems that existing ferrous sulphate and organic chelated iron have a poor stability or a poor greening - restoring effect, and easily produce adverse effects on quality of fruits, and the like.
To achieve above object, the invention uses following technical scheme:
An agent for restoring greening of yellow leaves of a fruit tree, comprising the following LU503859 components in parts by weight: 40 - 60 parts by weight of an iron and zinc greening - restoring agent, 5 - 10 parts by weight of calcium lignosulfonate, 3 - 5 parts by weight of boric acid, 1 - 3 parts by weight of sugar alcohol molybdenum, and 60 - 100 parts by weight of water.
The agent for restoring greening of yellow leaves of a fruit tree as above, wherein the iron and zinc greening - restoring agent is prepared by the following method: step A: adding chitosan in water and adjusting a pH to be acidic to obtain a chitosan solution; step B: dissolving ferrous sulphate and zinc sulphate in water, and adding hydroxylamine hydrochloride to obtain an iron and zinc mixed raw material solution; step C: dropwise adding the iron and zinc mixed raw material solution obtained in step B to the chitosan solution, stirring the solution while the dropwise adding, and after the dropwise adding is completed, continuously stirring the solution for a complexation reaction to obtain a mixed reaction system; and step D: adding acetone into the mixed reaction system for precipitation, and drying the obtained precipitate to obtain an iron and zinc greening - restoring agent.
The agent for restoring greening of yellow leaves of a fruit tree as above, wherein in step A, the chitosan has a deacetylation degree greater than or equal to 90% and a relative molecular weight of 2,000 - 4,000.
The agent for restoring greening of yellow leaves of a fruit tree as above, wherein in step A, the content of the chitosan in the chitosan solution is 20 - 50 g/l and the pH of the chitosan solution is adjusted to 3 - 5 by using a formic acid solution with a volume fraction of 1%.
The agent for restoring greening of yellow leaves of a fruit tree as above, wherein in step B, a ratio of the amount of zinc sulphate to ferrous sulphate is 1 : (10 - 15); and a concentration of a zinc ion in the iron and zinc mixed raw material solution is 4 - 10 mmol/l, and an initial concentration of the hydroxylamine hydrochloride is 5 - 15 g/l.
The agent for restoring greening of yellow leaves of a fruit tree as above, wherein in step A, a volume ratio of the chitosan solution in step A to the iron and zinc mixed raw material solution in step Bis 1: (0.6 - 1.0).
The agent for restoring greening of yellow leaves of a fruit tree as above, wherein in step C, the iron and zinc mixed raw material solution is dropwise added within 15 - 30 min and continuously stirred at room temperature for 2 - 6 h after the dropwise adding is completed.
The agent for restoring greening of yellow leaves of a fruit tree as above, wherein in step A, the chitosan is further pre-treated as follows: step (A-1): ultrasonically dispersing the chitosan in isopropanol to obtain a chitosan dispersion solution; step (A-2): slowly pouring the chitosan dispersion solution into a sodium hydroxide solution that is heated while stirred, and after the chitosan dispersion solution is added, continuously stirring the solution to obtain a mixed dispersion solution:
step (A-3): adding a chloroacetic acid solution into the mixed dispersion solution in batches and 503859 performing a heating reaction, and after the reaction is completed, adjusting a pH to neutral to obtain a mixed dispersion solution À; step (A-4): removing isopropanol in the mixed dispersion solution A by using a rotary evaporation method and adding distilled water to dissolve again to obtain a mixed dispersion solution B; and step (A-5): adding anhydrous ethanol to the mixed dispersion solution B for precipitation, and then successively performing suction filtration, washing, and drying to obtain the pre-treated chitosan.
The agent for restoring greening of yellow leaves of a fruit tree as above, wherein in step (A-1), the content of the chitosan in the chitosan dispersion is 80 - 200 g/l; in step (A-2), a volume ratio of the chitosan dispersion solution to the sodium hydroxide solution is (1 - 1.5):1 and a mass fraction of sodium hydroxide in the sodium hydroxide solution is 15 - 30 wt%, the heating is performed at a temperature of 40 - 50°C, and the continuous stirring is performed for 1 - 3 h; and in step (A-3), the chloroacetic acid solution is added in 3 - 5 times with an interval of 5 - 10 min, a ratio of a total volume of the added chloroacetic acid solution to a volume of the mixed dispersion solution is 1 : (2 - 4), the chloroacetic acid solution is obtained by dissolving from chloroacetic acid in isopropanol, the content of the chloroacetic acid in the chloroacetic acid solution is 500 - 800 g/l, and the heating reaction is performed at a temperature of 65 - 75°C for 5-6h.
A use method of an agent for restoring greening of yellow leaves of a fruit tree, wherein when yellow leaves appear on a fruit tree, the agent for restoring greening of yellow leaves of a fruit tree as above is diluted with water to 50 - 100 times and sprayed onto leaf surfaces of the fruit tree.
The technical solutions of the present disclosure have the following beneficial technical effects. 1. The agent for restoring greening of yellow leaves of a fruit tree of the present disclosure may rapidly restore greening of yellow leaves of a fruit tree, has a better effect of restoring greening, and may improve quality and yield of fruits. 2. The iron and zinc greening - restoring agent prepared by using the method of the present disclosure takes micromolecular chitosan with a deacetylation degree of more than or equal to 90% and a relative molecular weight of 2,000 - 4,000 as a carrier raw material, has a good biocompatibility after ferrous ions and zinc ions are chelated, may be quickly absorbed and utilized by yellow leaves of the fruit tree, may synergistically play a role with elements such as boron, molybdenum, calcium and the like, relieves a yellowing problem of the yellow leaves, and rapidly restore greening of the yellow leaves of the fruit tree.
3. Inthe present disclosure, the iron and zinc greening - restoring agent is prepared after the 503859 chitosan is pre-treated, such that the water solubility of the chitosan and the chelating ability of iron and zinc may be improved, which is probably caused by: a carboxymethyl substitution degree of the chitosan is related to the chelating ability of the chitosan to different metals, and the chitosan with the specific carboxymethyl substitution degree is prepared under a pre-treatment condition of the present disclosure, such that the chitosan is easier to chelate with ferrous ions and zinc ions, and the iron and zinc greening - restoring agent with a higher iron content is prepared. 4. Compared with an iron and zinc greening - restoring agent formed by mixing chelated iron and chelated zinc, the iron and zinc greening - restoring agent of the present disclosure has a better stability, which is mainly because the ferrous ions and the zinc ions are chelated by the iron and zinc greening - restoring agent at the same time, such that the ability of the iron and zinc greening - restoring agent for capturing other metal ions is weakened, and therefore, when the iron and zinc greening - restoring agent is mixed and dissolved with calcium lignosulfonate, boric acid, sugar alcohol molybdenum, water and the like, a uniform and stable dispersion solution may be formed, and an anti - flocculation ability of the agent for restoring greening of yellow leaves of a fruit tree is effectively improved. 5. Inthe present disclosure, zinc and ferrous ions with a specific ratio are added into the agent for restoring greening of yellow leaves of a fruit tree in a chelate form, such that an antagonistic absorption of zinc to iron may be avoided, and zinc elements may be supplemented to the yellow leaves of the fruit trees in time (the absorption bearing ability of the yellow leaves is obviously reduced compared with that of normal leaves, and thus the zinc elements cannot be absorbed in time), such that elements such as iron, zinc, calcium, boron, and molybdenum may cooperatively play a role in a plant body to promote the yellow leaves to rapidly restore greening, and growth and development of the fruit trees and fruits are improved.
Example 1
In the present example, the agent for restoring greening of yellow leaves of a fruit tree was prepared by mixing the following components in parts by weight: 50 parts by weight of an iron and zinc greening - restoring agent, 8 parts by weight of calcium lignosulfonate, 5 parts by weight of boric acid, 2 parts by weight of sugar alcohol molybdenum, and 80 parts by weight of water. The calcium lignosulfonate, the sugar alcohol molybdenum, and the boric acid are all commercially available, wherein the calcium lignosulfonate has an average relative molecular weight of 2,000 and the sugar alcohol molybdenum is a liquid agent with a molybdenum content greater than or equal to 20 g/l. The iron and zinc greening - restoring agent was prepared by the following method:
step A: chitosan was added in water and a pH was adjusted to be 4 by using a formic acid LU503859 solution with a volume fraction of 1% to obtain a chitosan solution, wherein the content of chitosan in the chitosan solution was 50 g/l and the chitosan used in the example had a deacetylation degree of 95.6% and a relative molecular weight of 2,000 - 3,000; 5 step B: ferrous sulphate and zinc sulphate were dissolved in water at an amount ratio of 1 : 10, and hydroxylamine hydrochloride was added to obtain an iron and zinc mixed raw material solution, wherein an initial concentration of the hydroxylamine hydrochloride in the iron and zinc mixed raw material solution was 8 g/l and a concentration of a zinc ion in the iron and zinc mixed raw material solution was 10 mmol/l; step C: the iron and zinc mixed raw material solution obtained in step B was dropwise added to the chitosan solution, the solution was stirred while dropwise added, the dropwise adding was completed within 15 min, and after the dropwise adding was completed, the solution was continuously stirred at room temperature for 4 h for a complexation reaction to obtain a mixed reaction system, wherein in the example, a volume ratio of the chitosan solution to the iron and zinc mixed raw material solution was 1 : 1; and step D: acetone was added into the mixed reaction system for precipitation, and the obtained precipitate was dried to obtain an iron and zinc greening - restoring agent.
Example 2
In the present example, the agent for restoring greening of yellow leaves of a fruit tree was prepared by mixing the following components in parts by weight: 60 parts by weight of an iron and zinc greening - restoring agent, 10 parts by weight of calcium lignosulfonate, 3 parts by weight of boric acid, 1 part by weight of sugar alcohol molybdenum, and 100 parts by weight of water. The calcium lignosulfonate, the sugar alcohol molybdenum, and the boric acid are all commercially available, wherein the calcium lignosulfonate has an average relative molecular weight of 2,000 and the sugar alcohol molybdenum is a liquid agent with a molybdenum content greater than or equal to 20 g/l. The method for preparing of the iron and zinc greening - restoring agent was the same as that in example 1. The difference lied that the chitosan used in the preparation of the iron and zinc greening - restoring agent in the example was pre-treated as follows before use: step (A-1): the chitosan was ultrasonically dispersed in isopropanol to obtain a chitosan dispersion solution, wherein the content of the chitosan in the chitosan dispersion solution was 100 g/l: step (A-2): the chitosan dispersion solution was slowly poured into a sodium hydroxide solution that was heated while stirred, and after the chitosan dispersion solution was added, the solution was continuously stirred for 1.5 h at a temperature kept at 45°C to obtain a mixed dispersion solution, wherein in the example, a volume ratio of the chitosan dispersion solution to the sodium hydroxide solution was 1: 1, and the LU503859 mass fraction of sodium hydroxide in the sodium hydroxide solution was 25 wt%; step (A-3): a chloroacetic acid solution was added into the mixed dispersion solution in 3 times with an interval of 5 min, a reaction system was heated to a temperature of 65°C for reaction for 5 h, and after the reaction was completed, a pH was adjusted to neutral to obtain a mixed dispersion solution A: step (A-4): isopropanol in the mixed dispersion solution À was removed by using a rotary evaporation method and distilled water was added to dissolve again to obtain a mixed dispersion solution B; and step (A-5): anhydrous ethanol was added to the mixed dispersion solution B for precipitation, and then suction filtration, washing, and drying were successively performed to obtain the pre-treated chitosan.
Example 3
In the present example, the agent for restoring greening of yellow leaves of a fruit tree was prepared by mixing the following components in parts by weight: 40 parts by weight of an iron and zinc greening - restoring agent, 5 parts by weight of calcium lignosulfonate, 4 parts by weight of boric acid, 1 part by weight of sugar alcohol molybdenum, and 60 parts by weight of water. The calcium lignosulfonate, the sugar alcohol molybdenum, and the boric acid are all commercially available, wherein the calcium lignosulfonate has an average relative molecular weight of 2,000 and the sugar alcohol molybdenum is a liquid agent with a molybdenum content greater than or equal to 20 g/l. The method for preparing the iron and zinc greening - restoring agent was different from example 1 only in that in step B, an amount ratio of the ferrous sulphate to zinc sulphate was 1 : 3.
Example 4
In the present example, the agent for restoring greening of yellow leaves of a fruit tree was prepared by mixing the following components in parts by weight: 60 parts by weight of an iron and zinc greening - restoring agent, 8 parts by weight of calcium lignosulfonate, 3 parts by weight of boric acid, 2 part by weight of sugar alcohol molybdenum, and 90 parts by weight of water. The calcium lignosulfonate, the sugar alcohol molybdenum, and the boric acid are all commercially available, wherein the calcium lignosulfonate has an average relative molecular weight of 2,000 and the sugar alcohol molybdenum is a liquid agent with a molybdenum content greater than or equal to 20 g/l. The method for preparing the iron and zinc greening - restoring agent was different from example 1 only in that an iron greening - restoring agent and a zinc greening - restoring agent were respectively prepared according to the method in example 1, when the iron greening - restoring agent was prepared, in step B, only the ferrous sulphate was added, but the zinc sulphate was not added; when the zinc greening - restoring agent was prepared was prepared, in step B, only the zinc sulphate was added, but the ferrous sulphate LU503859 was not added; and 55 parts by weight of the iron greening - restoring agent and 5 parts by weight of the zinc greening - restoring agent were mixed to obtain 60 parts by weight of the iron and zinc greening - restoring agent.
Comparative example 1
In the present comparative example, the agent for restoring greening of yellow leaves of a fruit tree was prepared by mixing the following components in parts by weight: 60 parts by weight of an iron greening - restoring agent, 7 parts by weight of calcium lignosulfonate, 4 parts by weight of boric acid, 3 parts by weight of sugar alcohol molybdenum, and 80 parts by weight of water. The calcium lignosulfonate, the sugar alcohol molybdenum, and the boric acid are all commercially available, wherein the calcium lignosulfonate has an average relative molecular weight of 2,000 and the sugar alcohol molybdenum is a liquid agent with a molybdenum content greater than or equal to 20 g/l. The method for preparing the iron greening - restoring agent was different from example 1 only in that in step B, only ferrous sulphate was added, but zinc sulphate was not added.
Comparative example 2
In the present comparative example, the agent for restoring greening of yellow leaves of a fruit tree was prepared by mixing the following components in parts by weight: 60 parts by weight of an iron and zinc greening - restoring agent, 12 parts by weight of calcium lignosulfonate, 2 parts by weight of boric acid, 4 parts by weight of sugar alcohol molybdenum, and 80 parts by weight of water. The calcium lignosulfonate, the sugar alcohol molybdenum, and the boric acid are all commercially available, wherein the calcium lignosulfonate has an average relative molecular weight of 2,000 and the sugar alcohol molybdenum is a liquid agent with a molybdenum content greater than or equal to 20 g/l. The method for preparing the iron and zinc greening - restoring agent was the same as that in example 1.
Comparative example 3
In the present comparative example, the agent for restoring greening of yellow leaves of a fruit tree was prepared by mixing the following components in parts by weight: 60 parts by weight of an iron and zinc greening - restoring agent, 3 parts by weight of calcium lignosulfonate, 8 parts by weight of boric acid, 0.5 part by weight of sugar alcohol molybdenum, and 80 parts by weight of water. The calcium lignosulfonate, the sugar alcohol molybdenum, and the boric acid are all commercially available, wherein the calcium lignosulfonate has an average relative molecular weight of 2,000 and the sugar alcohol molybdenum is a liquid agent with a molybdenum content greater than or equal to 20 g/l. The method for preparing the iron and zinc greening - restoring agent was the same as that in example 1.
The stability and the anti - flocculation ability of the agent for restoring greening of yellow LU503859 leaves of a fruit tree in examples 1 to 3 and comparative examples 1 to 3 were as follows: example 2 > example 1 > example 3 > example 4 > comparative example 2 > comparative example 3 > comparative example 1. The agents for restoring greening of yellow leaves of a fruit tree in examples 1 to 3 and comparative examples 1 to 3 were used to prevent and treat a yellow leaf disease of an apple tree to test a prevention and treatment effect on the yellow leaf disease. À test method was as follows: when yellow leaves appear on a fruit tree, the agent for restoring greening of yellow leaves of a fruit tree is diluted with water to 100 times and sprayed onto leaf surfaces of the fruit tree. Greening - storing effects of each agent for restoring greening of yellow leaves of a fruit tree were compared. The results were shown in Table 1.
Table 1
Compa- Compa- Compa- 1 2 3 4 example example | example 1 2 3
Greening -
A greening - restoring agent with clean water as a control group was used to spray yellow leaves of an apple tree. The results showed that compared with the control group, the fruit yield of the fruit tree sprayed with the agent for restoring greening of yellow leaves of a fruit tree was improved to different degrees, wherein the fruit tree in an example 2 group had a largest yield increase amplitude, the best fruit quality, and the most luxuriant branches and leaves.
Claims (10)
1. An agent for restoring greening of yellow leaves of a fruit tree, comprising the following components in parts by weight: 40 - 60 parts by weight of an iron and zinc greening - restoring agent, 5 - 10 parts by weight of calcium lignosulfonate, 3 - 5 parts by weight of boric acid, 1 - 3 parts by weight of sugar alcohol molybdenum, and 60 - 100 parts by weight of water.
2. The agent for restoring greening of yellow leaves of a fruit tree according to claim 1, wherein the iron and zinc greening - restoring agent is prepared by the following method: step A: adding chitosan in water and adjusting a pH to be acidic to obtain a chitosan solution; step B: dissolving ferrous sulphate and zinc sulphate in water, and adding hydroxylamine hydrochloride to obtain an iron and zinc mixed raw material solution; step C: dropwise adding the iron and zinc mixed raw material solution obtained in step B to the chitosan solution, stirring the solution while the dropwise adding, and after the dropwise adding is completed, continuously stirring the solution for a complexation reaction to obtain a mixed reaction system; and step D: adding acetone into the mixed reaction system for precipitation, and drying the obtained precipitate to obtain an iron and zinc greening - restoring agent.
3. The agent for restoring greening of yellow leaves of a fruit tree according to claim 2, wherein in step A, the chitosan has a deacetylation degree greater than or equal to 90% and a relative molecular weight of 2,000 - 4,000.
4. The agent for restoring greening of yellow leaves of a fruit tree according to claim 2, wherein in step A, the content of the chitosan in the chitosan solution is 20 - 50 g/l and the pH of the chitosan solution is adjusted to 3 - 5 by using a formic acid solution with a volume fraction of
1%.
5. The agent for restoring greening of yellow leaves of a fruit tree according to claim 2, wherein in step B, the ratio of the amount of zinc sulphate to ferrous sulphate is 1 : (10 - 15); and the concentration of the zinc ion in the iron and zinc mixed raw material solution is 4 - 10 mmol/l, and the initial concentration of the hydroxylamine hydrochloride is 5 - 15 g/l.
6. The agent for restoring greening of yellow leaves of a fruit tree according to claim 2, wherein in step A, the volume ratio of the chitosan solution in step A to the iron and zinc mixed raw material solution in step Bis 1: (0.6 - 1.0).
7. The agent for restoring greening of yellow leaves of a fruit tree according to claim 2, wherein 503859 in step C, the iron and zinc mixed raw material solution is dropwise added within 15 - 30 min and continuously stirred at room temperature for 2 - 6 h after the dropwise adding is completed.
8. The agent for restoring greening of yellow leaves of a fruit tree according to claim 2, wherein in step A, the chitosan is further pre-treated as follows: step (A-1): ultrasonically dispersing the chitosan in isopropanol to obtain a chitosan dispersion solution; step (A-2): slowly pouring the chitosan dispersion solution into a sodium hydroxide solution that is heated while stirred, and after the chitosan dispersion solution is added, continuously stirring the solution to obtain a mixed dispersion solution; step (A-3): adding a chloroacetic acid solution into the mixed dispersion solution in batches and performing a heating reaction, and after the reaction is completed, adjusting a pH to neutral to obtain a mixed dispersion solution A; step (A-4): removing isopropanol in the mixed dispersion solution A by using a rotary evaporation method and adding distilled water to dissolve again to obtain a mixed dispersion solution B; and step (A-5): adding anhydrous ethanol to the mixed dispersion solution B for precipitation, and then successively performing suction filtration, washing, and drying to obtain the pre-treated chitosan.
9. The agent for restoring greening of yellow leaves of a fruit tree according to claim 8, wherein — in step (A-1), the content of the chitosan in the chitosan dispersion is 80 - 200 g/l; — in step (A-2), a volume ratio of the chitosan dispersion solution to the sodium hydroxide solution is (1 - 1.5) : 1 and a mass fraction of sodium hydroxide in the sodium hydroxide solution is 15 - 30 wt%, the heating is performed at a temperature of 40 - 50°C, and the continuous stirring is performed for 1 - 3 h; and — in step (A-3), the chloroacetic acid solution is added in 3 - 5 times with an interval of 5 - 10 min, a ratio of a total volume of the added chloroacetic acid solution to a volume of the mixed dispersion solution is 1 : (2 - 4), the chloroacetic acid solution is obtained by dissolving from chloroacetic acid in isopropanol, the content of the chloroacetic acid in the chloroacetic acid solution is 500 - 800 g/l, and the heating reaction is performed at a temperature of 65 - 75°C for 5-6 h.
10. A use method of an agent for restoring greening of yellow leaves of a fruit tree, wherein when yellow leaves appear on a fruit tree, the agent for restoring greening of yellow leaves of a fruit tree according to any one of claims 1 - 9 is diluted with water to 50 - 100 times and Sprayed 03859 onto leaf surfaces of the fruit tree.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| LU503859A LU503859B1 (en) | 2023-04-05 | 2023-04-05 | Agent for restoring greening of yellow leaves of fruit tree, and use method thereof |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| LU503859A LU503859B1 (en) | 2023-04-05 | 2023-04-05 | Agent for restoring greening of yellow leaves of fruit tree, and use method thereof |
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| LU503859B1 true LU503859B1 (en) | 2023-10-06 |
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| LU503859A LU503859B1 (en) | 2023-04-05 | 2023-04-05 | Agent for restoring greening of yellow leaves of fruit tree, and use method thereof |
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| Country | Link |
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| LU (1) | LU503859B1 (en) |
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2023
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