LU501242B1 - Method for identifying salt-tolerant kiwifruit germplasm resources - Google Patents

Method for identifying salt-tolerant kiwifruit germplasm resources Download PDF

Info

Publication number
LU501242B1
LU501242B1 LU501242A LU501242A LU501242B1 LU 501242 B1 LU501242 B1 LU 501242B1 LU 501242 A LU501242 A LU 501242A LU 501242 A LU501242 A LU 501242A LU 501242 B1 LU501242 B1 LU 501242B1
Authority
LU
Luxembourg
Prior art keywords
salt
kiwifruit
tolerant
seedlings
leaves
Prior art date
Application number
LU501242A
Other languages
German (de)
Inventor
Yunpeng Zhong
Original Assignee
Zhengzhou Fruit Res Inst Caacultural Sciences
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Zhengzhou Fruit Res Inst Caacultural Sciences filed Critical Zhengzhou Fruit Res Inst Caacultural Sciences
Priority to LU501242A priority Critical patent/LU501242B1/en
Application granted granted Critical
Publication of LU501242B1 publication Critical patent/LU501242B1/en

Links

Classifications

    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H5/00—Angiosperms, i.e. flowering plants, characterised by their plant parts; Angiosperms characterised otherwise than by their botanic taxonomy
    • A01H5/08—Fruits
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H6/00—Angiosperms, i.e. flowering plants, characterised by their botanic taxonomy
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0018—Culture media for cell or tissue culture
    • C12N5/0025—Culture media for plant cell or plant tissue culture
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/04—Plant cells or tissues

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Botany (AREA)
  • Zoology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Physiology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Environmental Sciences (AREA)
  • Cultivation Of Plants (AREA)

Abstract

The invention simulates salt stress treatment of kiwifruit tissue culture potted seedlings by adding NaCl solution to the potted substrate until the quality of nacl accounts for 0.5% of the mass fraction of the substrate. after 30 days of treatment, the salt tolerance of kiwifruit germplasm resources is identified according to the salt damage index, and relates to the main steps of screening and evaluation of resistance resources of fruit trees include: Tissue culture of kiwifruit resources; rooting culture and transplantation of tissue cultured seedlings; NaCl stress treatment of tissue cultured potted seedlings; statistical analysis of salt damage index; identification of salt tolerance. The method is simple and easy to operate, because the selected materials are tissue-cultured potted seedlings with consistent growth, which can ensure the accuracy and reliability of the identification results, quickly screen salt-tolerant kiwifruit germplasm resources.

Description

DESCRIPTION 507242 Method for identifying salt-tolerant kiwifruit germplasm resources
TECHNICAL FIELD The invention relates to a method for identifying germplasm resources of fruit trees in the field of agricultural technology, and in particular provides a method for identifying salt-tolerance kiwifruit germplasm.
BACKGROUND Kiwifruit is an important fruit tree resource native to China. It is loved by people for its unique flavor and rich nutrients such as vitamin C. It is one of the four most successful wild fruit trees artificially domesticated and cultivated in the 20th century. China's kiwifruit cultivation area and output are among the top in the world, but the comprehensive quality, unit yield and price of the fruit are far behind those of developed countries such as New Zealand and Italy. One reasons is lacking compatible and resistant rootstock for kiwifruit. During the cultivation process, the rootstocks are often randomly selected from different strains of Actinidia chinensis or A. deliciosa, resulting in inconsistent fruit quality, such as uniform shape, single fruit weight, and taste, lacking market competitiveness and relatively low economic benefits.
Grafting propagation is the main propagation method for kiwifruit cultivation. Rootstock can not only improve the adaptability of grafted varieties to adversity (such as drought, salt-alkali, high temperature, waterlogging, etc.), but also adjust the growth characteristics, yield and fruit quality of the scion. Therefore, cultivating high-efficiency rootstocks with strong resistance and wide adaptability is very important to the development of kiwifruit resistance breeding and industry development in China.
Kiwifruit is a shallow-rooted plant. Most of its fleshy roots are distributed at a depth of 40 cm below the ground. They are very sensitive to salt stress. Salt-alkali stress can cause kiwifruit leaves to wilt and fall off. In severe cases, the stems dry up and their growth stagnates. The identification of resistance resources must first clarify the identification criteria for resistance. However, currently there 1s little research on salt tolerance of kiwifruit in China, and there is no uniform identification standard for 10501868 resistance resources. Therefore, it 1s urgent to establish a set of germplasm suitable for salt tolerance of kiwifruit, and identification methods for resource screening. In the process of long-term resource collection and resistance evaluation research, this research group has gradually summarized a set of effective salt-tolerance resource identification method of kiwifruit, and used this method to systematically evaluate the resistance of collected resources. At the same time, screening out salt-tolerance kiwifruit resources can promnote the research of grafting existing main varieties, conducting in-depth comparative studies, cultivating special salt-tolerant rootstocks, and accelarating the study work of kiwifruit resistance breeding and the development of the industry.
SUMMARY Aiming at the technical difficulties in the identification of fruit tree resistance resources, the present invention provides a method for identifying salt-tolerant kiwifruit germplasm resources. The invention is simple to operate, the required materials are easy to obtain, and the identification results are accurate and reliable. The present invention can quickly screen and identify resistance. Salt resources provide resistant materials for cultivating salt-tolerant rootstocks for kiwifruit. The salt tolerance identification method includes the following steps: 1) Tissue culture of kiwifruit resources; 2) Rooting culture and transplanting of tissue cultured seedlings; 3) NaCl stress treatment of tissue culture potted seedlings; 4) Statistical analysis of Salt Damage Index (SDI); 5) Identification of salt tolerance.
Preferably, the tissue culture method of the kiwifruit resource in step 1) is: select the newly sprouting shoots in spring, cut them into lengths of 15~40 cm, put them in clean water, and quickly bring them back to the tissue culture preparation room under shading; remove the leaves, leaving the petiole length about 1 cm, rinse under running water for 2 hours, cut into 3 cm long single bud stems, put on a clean bench, soak in 75% alcohol for 30 seconds, and soak in 0.1% mercury 1 Minutes, rinse with sterile water 5 times; cut off the two ends of the stem section about 0.2 cm in length, leaving 7501242 the petiole port about 0.4 cm, and inoculate it in the stem budding medium: MS + 0.5 mg/L 6-BA (6-benzyl adenine) + 1.0 mg/L IBA ( Indole acetic acid), adjust the pH of the medium to 5.8. The culture conditions are: 24+2° C, relative humidity 40%, 14 hours of light and 8 hours of darkness.
When the newly-born axillary buds on the stems grow to 2 cm, cut them off with a sterile scalpel and transfer them to the proliferation medium: MS + 1.0 mg/L ZT + 0.5 mg/L GA3 (gibberellin) (pH 5.8) ) To realize the multiplication and expansion of tissue culture seedlings;
Preferably, the rooting medium formula of the tissue cultured seedlings in step 2) is: MS + 0.7 mg/L.
IBA.
When the induced roots grow to about 2 cm, place them in the culture room and open the lid to refine the seedlings after 3 days.
Planting, the transplanting substrate is river sand: perlite: grass charcoal = 1:1:1, planted in a 32-hole plug tray, watered thoroughly before transplanting, watered a little after transplanting, and then sprayed regularly twice a day, each time continuously After 30 minutes, after the transplanted seedlings grow new leaves, carry out daily management;
Preferably, the NaCl stress treatment method of the tissue culture potted seedlings in step 3) is: when the tissue culture seedlings in the greenhouse plugs wait for the new branch vines in the spring of the next year, only one main vine is left and transplanted to the peat: Perlite: matrix=1:1:1 in a black nutrient bowl (18x18), weigh the weight of the substrate in the nutrient bowl with a balance before transplanting.
When the main vine grows to a height of 40 cm, select the growth For potted seedlings that are basically the same, add NaCl solution, and place a plastic disc under the nutrient bowl.
If there is any solution, pour it back into the nutrient bowl again to ensure that the added NaCl is 0.5% of the weight of the nutrient bowl’s substrate.
Water after 3 days of treatment, and then carry out daily management;
Preferably, the statistical analysis method of the salt damage index in step 4) is: days after salt stress, statistical analysis is performed according to the damage symptoms of the potted seedlings.
The grading standard of the salt damage index (SDI) is as follows:
I Both old leaves and new leaves (leaves on newly emerging shoots) grow 507242 normally, without symptoms of salt damage; II The old leaves turn yellow, mainly in the leaf margin and tip, and the new leaves grow normally; III The old leaves are yellow with dry symptoms, the dry area is less than 1/3, and the tender shoots of new leaves are accompanied by dry symptoms; IV The old leaves are yellow and dry, the dry area is more than 1/3, and the young shoots of the new leaves are dry; V The old leaves are almost completely dry and fall off, and the main vines are accompanied by dry symptoms.
Calculate the salt damage index of each kiwifruit resource according to the following formula: SDI=) (x>xn)/(5N)>x100 x--the value of salt hazard at all levels n--Number of plants at all levels of salt damage N--the total number of plants investigated Preferably, the salt tolerance identification in step 5) is based on the SDI value: the larger the value, the more severe the salt damage and the less salt-tolerant; the smaller the value, the lighter the salt damage and the more salt-tolerant. Salt hazard index 20<SDI<40 is a salt-tolerant resource; 40<SDI<60 is a moderate salt-tolerant resource; 60<SDI<80 is a salt-sensitive resource; 80<SDI<100 is a salt-sensitive resource.
The main advantage of the present invention is that when identifying salt-tolerant kiwifruit resources, the required identification materials are described in detail, the consistency of the identification materials can be ensured, and the corresponding salt damage index grading standards are formulated. Since kiwi is a dioecious plant, it will cause differences in seed quality (such as germination rate, seed content, etc.) due to sufficient pollination; If the branches are directly used for resource identification, the identification materials will be inconsistent due to factors such as the age, thickness of the branches, and new and old branches, which will affect the identification results. Therefore, the seeds and branches of kiwifruit are not ideal materials for the identification of resistance resources. Tissue culture can not only ensure the stability 7501242 and consistency of kiwifruit resources, but also maintain its excellent properties.
Therefore, kiwifruit resources are tissue cultured, rooted and transplanted. After winter dormancy, only one will be left after new germination in the spring of the second year. The main vine, and select materials with consistent growth (to ensure that the root growth is basically the same) for salt tolerance identification, which can ensure the uniformity of the identified materials to the greatest extent, combined with the statistics of the salt damage index of each resource after 30 days of NaCl treatment Analysis to ensure the stability and reliability of the appraisal results.
DESCRIPTION OF THE INVENTION Example In this embodiment, a method for identifying salt-tolerant kiwifruit germplasm resources, the method for identifying salt-tolerance includes the following steps Step: 1) Tissue culture of kiwi fruit resources; 2) Rooting culture and transplanting of tissue cultured seedlings; 3) NaCl stress treatment of tissue culture potted seedlings; 4) Statistical analysis of Salt Damage Index (SaltDamageIndex, SDI); 5) Salt tolerance identification.
Step 1) The tissue culture method of the medium kiwifruit resource is: select the newly sprouting shoots in spring, cut them into a length of 15-40 cm, put them in clean water, and quickly bring them back to the tissue culture preparation room under shade, and cut off the leaves. Leave the petiole with a length of about 1 cm, rinse it under running water for 2 hours, cut it into 3 cm long single bud stems, and place it on an ultra-clean workbench. Step 1) The tissue culture method of the medium kiwifruit resource is: select the newly sprouting shoots in spring, cut them into a length of 15-40 cm, put them in clean water, and quickly bring them back to the tissue culture preparation room under shade, and cut off the leaves. Leave the petiole with a length of about 1 cm, rinse it under running water for 2 hours, cut it into 3 cm long single bud stems, and place it on an ultra-clean workbench. MS+0.5mg/L6-BA (6-benzyl adenine)+1.0mg/LIBA (indole acetic acid), adjust the pH of the medium to 5.8. The 7501242 culture conditions are: 24+2° C, relative humidity 40%, 14 hours of light, and 8 hours of darkness.
When the newly born axillary buds on the stems grow to 2 cm, cut them off with a sterile scalpel, and transfer them to a pH 5.8 proliferation medium: MS+1.0mg/LZT (Zeatin)+0.5mg/LGA3 (Gibberellin) ), to realize the multiplication and expansion of tissue culture seedlings;
Step 2) The formula of the rooting medium for the tissue culture seedlings is: MS+0.7mg/LIBA (indole acetic acid). When the induced roots grow to about 2 cm, place them in the culture room and open the lid to refine the seedlings for 3 days.
Transplanting, the transplanting substrate is river sand: perlite: peat =1:1:1, planted in a 32-hole plug tray, watered thoroughly before transplanting, watered a little after transplanting, and then sprayed regularly twice a day, each time Continue for 30 minutes, after the transplanted seedlings grow new leaves, carry out daily management;
Step 3) The NaCl stress treatment method of the middle tissue culture potted seedlings is: when the tissue culture seedlings in the greenhouse plugs wait for the new branch vines in the next spring, only one main vine is left and transplanted to the peat: perlite: Substrate = 1:1:1 size 18*18 black nutrient bowl, medium, weigh the weight of the substrate in the nutrient bowl with a balance before transplanting, when the main vine grows to 40 cm high, select the basic growth For consistent potted seedlings, add NaCl solution, and place a plastic disc under the nutrient bowl.
If there is any solution that flows out, pour it back into the nutrient bowl again to ensure that the added NaCl is 0.5% of the weight of the nutrient bowl substrate.
NaCl treatment Watering after 3 days, then carry out daily management;
Step 4) The statistical analysis method of the salt damage index is: 30 days after NaCl stress treatment, statistical analysis is performed according to the damage symptoms shown by the potted seedlings.
The grading standard of the salt damage index (SDI) is as follows:
I Both old leaves and new leaves (leaves on newly emerging shoots) grow normally without salt damage;
II The old leaves turn yellow, mainly in the leaf margins and tip parts, and the 507242 new leaves grow normally; II The old leaves are yellow with dry symptoms, the dry area is less than 1/3, and the tender shoots of new leaves are accompanied by dry symptoms; IV The old leaves are yellow and dry, the dry area is more than 1/3, and the young shoots of the new leaves are dry; V The old leaves are almost completely dry and fall off, and the main vines are accompanied by dry symptoms.
Calculate the salt damage index of each kiwifruit resource according to the following formula: SDI=XZ(xXn)/(5N)X 100 x--the value of salt hazard at all levels n--Number of plants at all levels of salt damage N--the total number of plants investigated The basis for the identification of salt tolerance in step 5) is the SDI value: the larger the value, the more severe the salt damage and the less salt-tolerant; the smaller the value, the lighter the salt damage and the more salt-tolerant. Salt hazard index 20 <SDI<<40 is a salt-tolerant resource; 40 SDI<60 is a moderate salt-tolerant resource; 60<SDI<80 is a salt-sensitive resource; 80<<SDI<<100 is a salt-sensitive resource. Take a kind of kiwifruit resource identification according to the above method, go through tissue culture; rooting culture and transplanting of tissue cultured seedlings; take 49 sample tissues for NaCl stress treatment of tissue cultured potted seedlings, statistical analysis of salt damage index, of which the level I is 28 There are 18 for level II and 3 for level III, then SDIF(28*1+18*2+3*3)/(5*49)*100=29.8, so the kiwifruit resource is a salt-tolerant resource.
The preferred embodiments of the present invention have been described in detail above. It should be understood that many modifications and changes can be made according to the concept of the present invention without creative labor. Therefore, all technical solutions that can be obtained by those skilled in the art through logical analysis, reasoning or limited experiments based on the concept of the
. . . . 4e . LU501242 present invention on the basis of the prior art should fall within the protection scope determined by the claims.

Claims (6)

CLAIMS LU501242
1. An identification method for salt-tolerant kiwifruit germplasm resources, which mainly includes the following steps: 1) tissue culture of kiwifruit resources; 2) rooting culture and transplanting of tissue cultured seedlings; 3) NaCl stress treatment of tissue culture potted seedlings; 4) statistical analysis of Salt Damage Index (SDI); and 5) identification of salt tolerance.
2. The method for identifying germplasm resources of salt-tolerant kiwifruit according to claim 1, wherein the method for tissue culture of kiwifruit resources in step 1) is: selecting the twigs of kiwifruit that germinated that year, and cutting appropriate lengths to bring them back to tissue culture in the preparation room, cut off the leaves and petioles, rinse under running water for 1 to 2 hours, cut into 3 cm long single bud stems, and put them on a clean bench, soak in 75% alcohol for 30 seconds, and soak in 0.1% mercury; rinse 4 to 5 times with sterile water for 1 minute, cut off both ends of the stem and about 0.5 cm of the petiole port, and inoculate the stem germination medium: MS + 0.5 mg/L 6-BA (6-benzyl adenine) + 1.0 mg/L IBA (indole acetic acid), adjust the pH of the medium to 5.8; the culture conditions are: 24 +2° C, relative humidity 40%, 14 hours of light and 8 hours of darkness; when the newly born axillary buds on the stems grow to 2 cm, cut them and transfer them to the proliferation medium: MS + 1.0 mg/L ZT (Zeatin) (pH 5.8) or MS + 1.0 mg/L ZT +
0.5 mg/ L GAs (gibberellin) (pH 5.8), to realize the proliferation and expansion of tissue culture seedlings.
3. The method for identifying salt-tolerant kiwifruit germplasm resources according to claim 1, wherein the rooting medium formula of the tissue culture seedlings in step 2) is: MS + (0.2—0.7) mg/L. IBA, to be induced when the roots grow to about 2 cm, they can be transplanted after 2 to 4 days after opening the lid and refining the seedlings in the culture room; the transplanting substrate is river sand: perlite: grass charcoal=1:1:1, and planted in a 32-hole plug tray; in the medium, water thoroughly before transplanting, water a little after transplanting, and regularly spray water twice a day (sprinkler irrigation) afterwards; after the transplanted seedlings 507242 grow new leaves, carry out daily management.
4. The method for identifying salt-tolerant kiwifruit germplasm resources according to claim 1, wherein the NaCl stress treatment method of the tissue culture potted seedlings in step 3) is: the tissue culture seedlings placed in the greenhouse plugs and wait for the second when new vines are released in spring, only one main vine is left, transplanted into a black nutrient bowl (18x18) filled with grass charcoal: perlite: matrix=1:1:1, and weighed into the nutrient bowl with a balance when the main vine grows to a height of 40 cm, select potted seedlings that grow basically the same, add NaCl solution, and place a plastic disc under the nutrient bowl; if there is any solution that flows out, pour it back into the nutrient bowl again , to ensure that the added NaCl quality accounts for 0.5% of the weight of the nutrient bowl substrate, watering only 3 days after salt treatment, and then daily management.
5. The method for identifying salt-tolerant kiwifruit germplasm resources according to claim 1, wherein the statistical analysis method of the salt damage index in step 4) is: 30 days after salt stress, according to the damage symptoms of potted seedlings according to statistical analysis, the grading standards of the Salt Injury Index (SDI) are as follows: I both old leaves and new leaves (leaves on newly emerging shoots) grow normally, without symptoms of salt damage; II the old leaves turn yellow, mainly in the leaf margin and tip, and the new leaves grow normally; II the old leaves are yellow with dry symptoms, the dry area is less than 1/3, and the tender shoots of new leaves are accompanied by dry symptoms; IV the old leaves are yellow and dry, the dry area is more than 1/3, and the young shoots of the new leaves are dry; V the old leaves are almost completely dry and fall off, and the main vines are accompanied by dry symptoms; calculate the salt damage index of each kiwifruit resource according to the following formula: SDI=) (x>xn)/(5N)>x100 x--the value of salt hazard at all levels 7501242 n--number of plants at all levels of salt damage N--the total number of plants investigated.
6. The method for identifying salt-tolerant kiwifruit germplasm resources according to claim 1, wherein the salt-tolerant identification in step 5) is based on the SDI value: the larger the value, the more severe the salt damage and the less salt-tolerant; the smaller the value, the lighter the salt damage and the more salt-tolerant; salt hazard index 20<SDI<40 is a salt-tolerant resource; 40<SDI<60 is a moderate salt-tolerant resource; 60<SDI << 80 is a salt-sensitive resource; 80<SDI<100 is a salt-sensitive resource.
LU501242A 2022-01-13 2022-01-13 Method for identifying salt-tolerant kiwifruit germplasm resources LU501242B1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
LU501242A LU501242B1 (en) 2022-01-13 2022-01-13 Method for identifying salt-tolerant kiwifruit germplasm resources

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
LU501242A LU501242B1 (en) 2022-01-13 2022-01-13 Method for identifying salt-tolerant kiwifruit germplasm resources

Publications (1)

Publication Number Publication Date
LU501242B1 true LU501242B1 (en) 2022-07-13

Family

ID=82362385

Family Applications (1)

Application Number Title Priority Date Filing Date
LU501242A LU501242B1 (en) 2022-01-13 2022-01-13 Method for identifying salt-tolerant kiwifruit germplasm resources

Country Status (1)

Country Link
LU (1) LU501242B1 (en)

Similar Documents

Publication Publication Date Title
Webster Rootstock and interstock effects on deciduous fruit tree vigour, precocity, and yield productivity
Durand-Cresswell et al. Vegetative propagation of Eucalyptus
NL2027681B1 (en) In vitro propagation method of tissue culture seedlings of zanthoxylum armatum
Sunarti et al. Breeding strategy of Acacia Hybrid (Acacia mangium× A. auriculiformis) to increase forest plantation productivity in Indonesia
CN101536674A (en) Tissue culture reproduction method of Chinese evergreen
Lakshminarayana et al. Effect of orchard heating in reducing parthenocarpic fruits in'Haden'mango
CN103109747B (en) Rapid pseudolarix propagation method based on stem node propagation
CN113115654A (en) Grape double-stock green branch grafting method
Gimenes et al. Propagation of Cabralea canjerana by mini-cutting
Ozkaynak Tuber size effects on yield and number of potato minitubers of commercial varieties in a greenhouse production system
Souza et al. Production of pear trees grafted under hydroponic conditions.
CN106342555B (en) Method for breeding new species of natural hetero-alternating heterogeneous populus euphratica and populus griseus
Leakey et al. Precocious flowering and reproductive biology of Triplochiton scleroxylon K. Schum
LU501242B1 (en) Method for identifying salt-tolerant kiwifruit germplasm resources
Arce et al. Seasonality in rooting of Prosopis chilensis cuttings and in-vitro micropropagation
Heybroek et al. Juvenility in fruit growing and forestry
CN112931013A (en) Rapid propagation and culture method of excellent kiwi fruit stock
CN107926672B (en) Method for promoting bodhi water culture rooting
CN110622859A (en) High-bud induced seedling growing method for dendrobium devonianum
CA2935120A1 (en) Methods and compositions for production of watermelon fruit
KR100302206B1 (en) In-flight mass production and forge transplanting method
Balaj et al. The effect of rootstock on vegetative development and flower production in modern garden roses (Rosa canina L.).
SANADA et al. Bulbil dormancy and formation in water yam (Dioscorea alata L.)
Sudhersan et al. Introduction, evaluation and propagation of Ziziphus In Kuwait
Ranjith et al. Influence of top working on growth and flowering of tea clones

Legal Events

Date Code Title Description
FG Patent granted

Effective date: 20220713