LU101690B1 - Image filtering intensity enhancement apparatus for biological spectrum scanning technology - Google Patents
Image filtering intensity enhancement apparatus for biological spectrum scanning technology Download PDFInfo
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- LU101690B1 LU101690B1 LU101690A LU101690A LU101690B1 LU 101690 B1 LU101690 B1 LU 101690B1 LU 101690 A LU101690 A LU 101690A LU 101690 A LU101690 A LU 101690A LU 101690 B1 LU101690 B1 LU 101690B1
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- image filtering
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6445—Measuring fluorescence polarisation
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/645—Specially adapted constructive features of fluorimeters
- G01N21/6456—Spatial resolved fluorescence measurements; Imaging
- G01N21/6458—Fluorescence microscopy
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6486—Measuring fluorescence of biological material, e.g. DNA, RNA, cells
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Abstract
The present invention discloses an image filtering intensity enhancement apparatus for a biological spectrum scanning technology. The apparatus comprises a CCD camera, two polarizers, a dichroic beam splitter, a laser device, a plurality of lenses, a diffuser, and an engine connected to the diffuser. Through the polarized fluorescence spectrum image filtering intensity enhancement technology, a fluorescence spectrum image with weak signals or background light interference is enhanced, so that the fluorescence spectrum image is enhanced, can better reflect the characteristics of cervical cancer, and thus can provide a support for the location and identification of late cervical cancer lesions.
Description
| 1 / IMAGE FILTERING INTENSITY ENHANCEMENT APPARATUS FOR BIOLOGICAI-U101690
SPECTRUM SCANNING TECHNOLOGY Field of the Invention The present invention relates to a novel polarized fluorescence spectrum image filtering intensity enhancement apparatus for a non-invasive cervical cancer early screening device based on a biological spectrum scanning technology. Background of the Invention Cervical cancer is one of the most common malignancies, and the occurrence of cervical cancer in China ranks first in female reproductive tract malignancies. In China, the number of cervical cancer cases is about 130,000 and about 50,000 die each year. The cure rate of cervical cancer can reach 100% via early detection. Therefore, early screening, early detection and early treatment have very important practical significance for curing the cervical cancer. In recent years, photon imaging, which is a new non-destructive biomedical imaging technology, has developed rapidly. It combines the advantages of pure optical imaging and pure ultrasonic imaging to provide high-resolution and high-contrast tissue imaging. As a new generation of biomedical imaging technology, the photon imaging can effectively perform biological tissue structure and function imaging, thereby providing an important means for studying the morphological structures, physiological characteristics, pathological characteristics, and metabolic functions of biological tissues. The inventors found that the traditional biophoton imaging technology is less sensitive to weak-intensity biochemical signals, is susceptible to interference from background light, cannot reflect the characteristics of cervical cancer, and is insufficient in extracted fluorescence spectrum features, which affects the precision of identification. At present, effective detection and extraction means lack for the acquisition and processing data of cervical fluorescence spectra, and late cervical cancer lesions cannot be effectively located and identified. Summary of the Invention In order to solve the technical problems in the prior art, the present invention aims to provide an image filtering intensity enhancement apparatus for a biological spectrum scanning technology. Through the polarized fluorescence spectrum image filtering intensity enhancement technology, a Ta
| ; fluorescence spectrum image with weak signals or background light interference is enhanced, sd-thd}1690 , the fluorescence spectrum image is enhanced, can better reflect the characteristics of cervical cancer, and thus can provide a support for the location and identification of late cervical cancer lesions. ; In order to achieve the above objectives, the present invention adopts the following technical solution: An image filtering intensity enhancement apparatus for a biological spectrum scanning technology disclosed in one or more embodiments, includes: a camera, a first polarizer, a second polarizer, a ; dichroic beam splitter, a laser device, a first lens, a second lens, a third lens, a fourth lens, a fifth lens, a diffuser, and an engine connected to the diffuser; / 10 laser generated by the laser device passes through the first lens, the second lens, the diffuser, the first / polarizer, the third lens, the fourth lens, and the dichroic beam splitter in sequence, then a part of the laser is irradiated to a sample, and the other part passes through the third lens and the second polarizer and enters the CCD camera.
This design is used to enhance a fluorescence spectrum image with weak signals or background light , 15 interference, so as to provide a support for the location and identification of late cervical cancer lesions.
A further scheme of the present invention is: the polarizer has a function of shielding and transmitting incident light, and can transmit one of longitudinal light and transverse light and shield | the other one. , 20 A further scheme of the present invention is: the two polarizers are placed orthogonally. | A further scheme of the present invention is: the fluorescence radiated by a sample S under excitation is natural light, and the part of the fluorescence polarized parallel to the first polarizer can pass through the first polarizer successfully and be incident on the camera. | A further scheme of the present invention is: the diffuser is used to uniformly diffuse the laser to be a plane, and the laser is uniformly irradiated on the sample through the lenses and the dichroic beam splitter, thereby exciting the sample to emit fluorescence. | A further scheme of the present invention is: the polarization direction of the first polarizer is orthogonal to the direction of laser, so the laser cannot pass through the first polarizer and can be : filtered, but the fluorescence emitted by the sample is natural light and can pass through the first | 30 polarizer.
A further scheme of the present invention is: the polarization direction of the second polarizer is orthogonal to the first polarizer, the laser passes through the second polarizer to generate laser parallel to the second polarizer, and the laser can excite the sample to emit fluorescence and meet the | above requirements, so that the laser cannot pass through the first polarizer.
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A further scheme of the present invention is: the first lens, the second lens, the third lens, the fouktH101690 lens, and the fifth lens are all convex lenses.
Beneficial effects of the invention: In the present invention, laser passes through the polarizer to form linear laser, which can enhance the fluorescence spectrum image after being irradiated to the sample.
In addition, because the laser is linear and the sample fluorescence is natural light, the interference of the laser to the fluorescence spectrum image can be eliminated by the polarizer P1. To sum up, this apparatus enhances the fluorescence spectrum image with weak signals or background light interference, so that the fluorescence spectrum image is enhanced, can better reflect the characteristics of cervical cancer, and thus can locate and identify late cervical cancer lesions.
Brief Description of the Drawings The accompanying drawings constituting a part of the present application are used for providing a further understanding of the present application, and the schematic embodiments of the present application and the description thereof are used for interpreting the present application, rather than | constituting improper limitations to the present application. | Fig. 1 is a schematic diagram of a polarization CCD; | Fig. 2 is a schematic diagram of the principle of orthogonal polarization filtering; In the figures: 1 laser, 2 lens, 3 lens, 4 diffuser, 5 engine, 6 lens, 7 lens, 8 lens, 9 camera, 10 dichroic | beam splitter, 11 sample, P2 polarizer, P1 polarizer. | Detailed Description of Embodiments | It should be pointed out that the following detailed descriptions are all exemplary and aim to further | illustrate the present application.
Unless otherwise specified, all technological and scientific terms | used in the present invention have the same meanings generally understood by those of ordinary skill | in the art of the present application. / It should be noted that the terms used herein are merely for describing specific embodiments, but are not intended to limit exemplary embodiments according to the present application.
As used herein, unless otherwise explicitly pointed out by the context, the singular form is also intended to include the plural form.
In addition, it should also be understood that when the terms “include” and/or : “comprise” are used in the description, they indicate features, steps, operations, devices, components Ve aand/or their combination.
The following clearly and completely describes the technical solutions in 01690 the present invention with reference to the accompanying drawings in the present invention.
A polarized fluorescence spectrum image filtering intensity enhancement system for a cervical cancer early screening biological spectrum scanning technology proposed in this embodiment includes a CCD camera 9, a polarizer P1, a polarizer P2, a dichroic beam splitter 10, a laser device 1, a plurality of lenses (lens 2, lens 3, lens 6, lens 7, lens 8), a diffuser 4, and an engine 5 connected to the diffuser; Specifically, five lenses are provided, respectively a first convex lens 2, a second convex lens 3, a third convex lens 6, a fourth convex lens 7, and a fifth convex lens 8; i laser generated by the laser device passes through the first convex lens, the second convex lens, the diffuser, the first polarizer P1, the third convex lens, the fourth convex lens, and the dichroic beam splitter in sequence, then a part of the laser is irradiated to a sample, and the other part passes through the third convex lens and the second polarizer P2 and enters the CCD camera. | This design is used to enhance a fluorescence spectrum image with weak signals or background light | interference, so as to provide a support for the location and identification of late cervical cancer { lesions. | A further scheme of the present invention is: the polarizer has a function of shielding and | transmitting incident light, and can transmit one of longitudinal light and transverse light and shield ] the other one.
The polarizer is made of a composite material formed by laminating a polarizing film, | an inner protective film, a pressure-sensitive adhesive layer, and an outer protective film. | The fluorescence radiated by a sample S under excitation is natural light, and the part of the | fluorescence polarized parallel to the first polarizer can pass through the first polarizer successfully | and be incident on the CCD camera. | The diffuser is used to uniformly diffuse the laser to be a plane, and the laser is uniformly irradiated / on the sample through the lenses and the dichroic beam splitter, thereby exciting the sample to emit | fluorescence. | The dichroic beam splitter is an existing beam splitter, and details are not described herein again. | In the present invention, an engine is mounted under the diffuser to adjust the angle of the diffuser, J so that the diffuser generates a laser beam incident on the polarizer P2 perpendicularly. 1 The two polarizers are placed orthogonally.
The polarization direction of the first polarizer is | Pa EEE EEE EN NEE A FEorthogonal to the direction of laser, so the laser cannot pass through the first polarizer and can-Bé01830 filtered, but the fluorescence emitted by the sample is natural light and can pass through the first polarizer.
The polarization direction of the second polarizer is orthogonal to the first polarizer, the laser passes 5 through the second polarizer to generate laser parallel to the second polarizer, and the laser can excite the sample to emit fluorescence and meet the above requirements, so that the laser cannot pass through the first polarizer.
In this embodiment, the CCD camera image of the apparatus has more than 380,000 dots, a color | resolution of more than or equal to 480 lines, a black and white resolution, and a high resolution of more than 600 lines, the illuminance required for normal operation is less than 0.01x, and the lin target surface has a width of 12.7 mm, a height of 9.6 mm and a diagonal of 16 mm.
An image of fluorescence spectra is divided into two spectrum windows, one of which is the reflected and scattered light of exciting light, and the other is fluorescence.
Aiming at the problems that the fluorescence has weak spectra and is vulnerable to background signal interference, the intensity of a spectrum image is improved from both software and hardware algorithms.
In this embodiment, an enhanced CCD is used in terms of hardware to supplement light when scanning tissues.
The operating principle of the filtering scheme based on orthogonal polarization is explained as follows: the optical path in Fig. 1 is expanded (only considering the polarization characteristics of light) to obtain a simplified schematic diagram of the optical path principle shown in Fig. 2, where the dotted lines with arrows on a reference plane 1 and the solid line with arrows on a reference plane 2 represent the polarization directions of light, the solid lines with arrows on the polarizers P1 and P2 represent the polarization directions of the polarizers P1 and P2, and the dotted lines and solid lines with arrows on the sample S in Fig. 2 represent the polarization directions of exciting light and fluorescence, respectively.
Specifically, the reference plane 1 is at any position in front of the polarizer P2 on which exciting light (the dotted lines with arrows indicate the exciting light, and the arrows indicate the polarization directions) is incident, the polarization state of the exciting light is natural polarization, the exciting light passing through the polarizer P2 (the polarization direction is shown in Fig. 2) and incident on the sample 11 is linearly polarized light, the sample 11 is excited by the exciting light to emit a fluorescent signal (the red solid line with arrows represents fluorescence, , aaa ae a eEEEEEEEETTTTTTT
| 6 and the arrows represent the polarization direction), and the fluorescence is natural light or Her 1690 natural light.
Because the polarizer P1 is orthogonal to the polarizer P2, the exciting light having its | polarization direction perpendicular to the polarization direction of the polarizer is filtered when , passing through the polarizer P1. The fluorescence radiated by the sample 11 under excitation is | 5 natural light, and the part of the fluorescence polarized parallel to the polarizer P1 can pass through the polarizer P1 successfully and be incident on the CCD camera. | In the present invention, laser passes through the polarizer to form linear laser, which can enhance | the fluorescence spectrum image after being irradiated to the sample.
In addition, because the laser is linear and the sample fluorescence is natural light, the interference of the laser to the fluorescence spectrum image can be eliminated by the polarizer P1. Based on the above, this apparatus enhances | the fluorescence spectrum image with weak signals or background light interference, so that the fluorescence spectrum image is enhanced, can better reflect the characteristics of cervical cancer, and thus can locate and identify late cervical cancer lesions. . Described above are merely preferred embodiments of the present application, and the present application is not limited thereto.
Various modifications and variations may be made to the present application for those skilled in the art.
Any modification, equivalent substitution, improvement or the like made within the spirit and principle of the present application shall fall into the protection scope of the present application. | 20
Claims (7)
1. An image filtering intensity enhancement apparatus for a biological spectrum scanning technology, comprising: a CCD camera, a first polarizer, a second polarizer, a dichroic beam splitter, a laser device, a first lens, a second lens, a third lens, a fourth lens, a fifth lens, a diffuser, and an engine connected to the diffuser; wherein laser generated by the laser device passes through the first lens, the second lens, the diffuser, the first polarizer, the third lens, the fourth lens, and the dichroic beam splitter in sequence, then a part of the laser is irradiated to a sample, and the other part passes through the third lens and the second polarizer and enters the CCD camera.
2. The image filtering intensity enhancement apparatus for a biological spectrum scanning technology according to claim 1, wherein the two polarizers are placed orthogonally.
3. The image filtering intensity enhancement apparatus for a biological spectrum scanning technology according to claim 1, wherein the fluorescence radiated by a sample under excitation is natural light, and the part of the fluorescence polarized parallel to the first polarizer can pass through the first polarizer successfully and be incident on the CCD camera.
4. The image filtering intensity enhancement apparatus for a biological spectrum scanning technology according to claim 1, wherein the diffuser is used to uniformly diffuse the laser to be a plane, and the laser is uniformly irradiated on the sample through the third lens, the fourth lens and the dichroic beam splitter, thereby exciting the sample to emit fluorescence.
5. The image filtering intensity enhancement apparatus for a biological spectrum scanning technology according to claim 1, wherein the polarization direction of the first polarizer is orthogonal to the direction of laser.
6. The image filtering intensity enhancement apparatus for a biological spectrum scanning technology according to claim 5, wherein the polarization direction of the second polarizer is orthogonal to the first polarizer, and the laser passes through the second polarizer to generate laser parallel to the second polarizer.
7. The image filtering intensity enhancement apparatus for a biological spectrum scanning technology according to claim 1, wherein the first lens, the second lens, the third lens, the fourth lens, and the fifth lens are all convex lenses.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910425248 | 2019-05-21 | ||
| CN201910605157.XA CN110286108A (en) | 2019-05-21 | 2019-07-05 | A kind of image filtering Strength reinforcement device for biological spectrum scanning technique |
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| CN111366569A (en) * | 2020-05-09 | 2020-07-03 | 杭州希浦芯光电科技有限公司 | Portable optical detector |
| CN111426670A (en) * | 2020-05-09 | 2020-07-17 | 合肥法拉第光电科技有限公司 | Portable optical detector |
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| JP5058815B2 (en) * | 2004-11-24 | 2012-10-24 | バッテル メモリアル インスティチュート | Optical system for cell imaging |
| CN102175656A (en) * | 2010-12-30 | 2011-09-07 | 深圳大学 | Fluorescence microscopic imaging method and system |
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