LT7204B - Vertingų medžiagų ekstrakcijos iš ląstelių laidžioje terpėje prietaisas ir būdas - Google Patents

Vertingų medžiagų ekstrakcijos iš ląstelių laidžioje terpėje prietaisas ir būdas

Info

Publication number
LT7204B
LT7204B LT2024546A LT2024546A LT7204B LT 7204 B LT7204 B LT 7204B LT 2024546 A LT2024546 A LT 2024546A LT 2024546 A LT2024546 A LT 2024546A LT 7204 B LT7204 B LT 7204B
Authority
LT
Lithuania
Prior art keywords
cells
medium
suspension
channel
channels
Prior art date
Application number
LT2024546A
Other languages
English (en)
Other versions
LT2024546A (lt
Inventor
Voitech STANKEVIČ
Arūnas STIRKĖ
Adei Hussam Mohamed Gabr ABOUHAGGER
Original Assignee
Valstybinis mokslinių tyrimų institutas Fizinių ir technologijos mokslų centras
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Valstybinis mokslinių tyrimų institutas Fizinių ir technologijos mokslų centras filed Critical Valstybinis mokslinių tyrimų institutas Fizinių ir technologijos mokslų centras
Priority to LT2024546A priority Critical patent/LT7204B/lt
Priority to EP25222226.0A priority patent/EP4759916A1/en
Publication of LT2024546A publication Critical patent/LT2024546A/lt
Publication of LT7204B publication Critical patent/LT7204B/lt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/02—Form or structure of the vessel
    • C12M23/16—Microfluidic devices; Capillary tubes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/04—Filters; Permeable or porous membranes or plates, e.g. dialysis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M35/00—Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for electroporation or cell fusion
    • C12M35/02—Electrical or electromagnetic means, e.g. for electroporation or for cell fusion
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M35/00—Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for electroporation or cell fusion
    • C12M35/04—Mechanical means, e.g. sonic waves, stretching forces, pressure or shear stimuli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/40—Means for regulation, monitoring, measurement or control, e.g. flow regulation of pressure
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass

Landscapes

  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Sustainable Development (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Analytical Chemistry (AREA)
  • Clinical Laboratory Science (AREA)
  • Dispersion Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Electromagnetism (AREA)
  • Mechanical Engineering (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)

Abstract

Išradimas susijęs su mikrofluidiniu prietaisu, skirtu efektyviau išskirti vertingus komponentus iš biologinių ląstelių, esančių terpėse su dideliu elektriniu laidumu. Prietaisas susideda iš dviejų kanalų (4, 5), serpantininio maišytuvo (12), apdorojimo elektriniais impulsais zonos (6) ir pasyvaus kintamo pločio kanalo grandininio maišytuvo (13). Per vieną kanalą (4) tiekiama suspensija ‒ terpė su apdorojamomis ląstelėmis (15), o per kitą kanalą (5) – terpė (16). Kanalai (4) ir (5) yra įrengti vienas virš kito ir apdorojimo elektriniais impulsais zonoje (6) šie kanalai susijungia per porėtą padėklą (3), kuris nepraleidžia ląstelių. Šioje zonoje įrengti du plokštieji elektrodai: viršutinis elektrodas (7a), įrengtas virš viršutinio kanalo (4), ir apatinis elektrodas (7b), įrengtas žemiau apatinio kanalo (5). Dėl skirtingų suspensijos ir terpės tekėjimo greičių porėto padėklo (3) paviršiuje susidaro padidintos koncentracijos ląstelių sluoksnis (14), pasižymintis didesne nei terpės elektrine varža. Pridėjus prie elektrodų impulsinę įtampą, stipriausias elektrinis laukas formuojasi būtent šio sluoksnio (14) paviršiuje. Tai užtikrina efektyvią ląstelių permeabilizaciją net ir aukšto laidumo suspensijose, pavyzdžiui, jūrinių dumblių terpėje. Po ląstelių permeabilizacijos, suspensijai tekant per pasyvų kintamo pločio kanalo grandininį maišytuvą (13), vyksta viduląstelinių komponentų (17) išskyrimas. Šie komponentai (17) praeina per porėtą padėklą (3) ir kartu su terpe išleidžiami iš prietaiso. Tokia prietaiso konstrukcija sumažina suspensijos šuntavimo efektą ir padidina vertingų medžiagų ekstrakcijos iš ląstelių efektyvumą laidžioje terpėje.
LT2024546A 2024-12-12 2024-12-12 Vertingų medžiagų ekstrakcijos iš ląstelių laidžioje terpėje prietaisas ir būdas LT7204B (lt)

Priority Applications (2)

Application Number Priority Date Filing Date Title
LT2024546A LT7204B (lt) 2024-12-12 2024-12-12 Vertingų medžiagų ekstrakcijos iš ląstelių laidžioje terpėje prietaisas ir būdas
EP25222226.0A EP4759916A1 (en) 2024-12-12 2025-12-10 Device and method for permeabilisation of biological cells in conductive medium

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
LT2024546A LT7204B (lt) 2024-12-12 2024-12-12 Vertingų medžiagų ekstrakcijos iš ląstelių laidžioje terpėje prietaisas ir būdas

Publications (2)

Publication Number Publication Date
LT2024546A LT2024546A (lt) 2026-06-25
LT7204B true LT7204B (lt) 2026-08-10

Family

ID=97915440

Family Applications (1)

Application Number Title Priority Date Filing Date
LT2024546A LT7204B (lt) 2024-12-12 2024-12-12 Vertingų medžiagų ekstrakcijos iš ląstelių laidžioje terpėje prietaisas ir būdas

Country Status (2)

Country Link
EP (1) EP4759916A1 (lt)
LT (1) LT7204B (lt)

Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4910140A (en) 1988-04-18 1990-03-20 Bio-Rad Laboratories, Inc. Electroporation of prokaryotic cells
US5690978A (en) 1996-09-30 1997-11-25 Ohio State University High voltage pulsed electric field treatment chambers for the preservation of liquid food products
US9029108B2 (en) 2009-11-06 2015-05-12 Diversified Technologies, Inc. Pulsed electric field (PEF) method for continuous enhanced extraction of oil and lipids from small aquatic plants
WO2015181322A1 (en) 2014-05-29 2015-12-03 Consejo Superior De Investigaciones Científicas (Csic) Device for measuring the trans-layer electrical impedance in an in vitro model of a cell barrier
US20200115668A1 (en) 2016-12-22 2020-04-16 The Charles Stark Draper Laboratory, Inc. System and method of using a microfluidic electroporation device for cell treatment
US11377652B2 (en) 2018-07-09 2022-07-05 NanoCav, LLC Micro flow-through electroporation devices and methods of cell transfection
US11724213B2 (en) 2020-02-27 2023-08-15 Lawrence Livermore National Security, Llc Modular, disposable 3D printed microfluidic membrane system for separation and purification

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2013013304A1 (en) * 2011-07-25 2013-01-31 Qvella Corporation Methods and devices for electrical sample preparation
US12472495B2 (en) * 2020-10-28 2025-11-18 Northwestern University Microfluidic device for live cell manipulation and analysis

Patent Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4910140A (en) 1988-04-18 1990-03-20 Bio-Rad Laboratories, Inc. Electroporation of prokaryotic cells
US5690978A (en) 1996-09-30 1997-11-25 Ohio State University High voltage pulsed electric field treatment chambers for the preservation of liquid food products
US9029108B2 (en) 2009-11-06 2015-05-12 Diversified Technologies, Inc. Pulsed electric field (PEF) method for continuous enhanced extraction of oil and lipids from small aquatic plants
WO2015181322A1 (en) 2014-05-29 2015-12-03 Consejo Superior De Investigaciones Científicas (Csic) Device for measuring the trans-layer electrical impedance in an in vitro model of a cell barrier
US20200115668A1 (en) 2016-12-22 2020-04-16 The Charles Stark Draper Laboratory, Inc. System and method of using a microfluidic electroporation device for cell treatment
US11377652B2 (en) 2018-07-09 2022-07-05 NanoCav, LLC Micro flow-through electroporation devices and methods of cell transfection
US11724213B2 (en) 2020-02-27 2023-08-15 Lawrence Livermore National Security, Llc Modular, disposable 3D printed microfluidic membrane system for separation and purification

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
ODIJK, VAN DER MEER, LEVNER, KIM, VAN DER HELM, SEGERINK, FRIMAT, HAMILTON, INGBER, VAN DEN BERG: "Measuring direct current trans-epithelial electrical resistance in organ-on-a-chip microsystems", LAB ON A CHIP

Also Published As

Publication number Publication date
EP4759916A1 (en) 2026-06-17
LT2024546A (lt) 2026-06-25

Legal Events

Date Code Title Description
BB1A Patent application published

Effective date: 20260625

FG9A Patent granted

Effective date: 20260810