LT5317B - Cow fodder for improving quality of milk - Google Patents

Abstract

The present invention relates to a dairy cows fodder composition which improving quality of milk by reducing somatic cells quantity in milk. The composition comprises fermentative additive of 100 to 500 mg/kg of animal body weight of preparation lizocime G3x, or of 10 to 50 mg/kg of animal body weight of preparation lizobutiline G10x, or 200 mg/kg of animal body weight of preparation lizocime G3x and 20 mg/kg of animal body weight of preparation lizobutiline G10x. The milk from cows so fed by said composition for up to 10 days contains somatic cells less than 3 times.

Description

The present invention relates to a cow's feed composition which improves milk quality by reducing the amount of somatic cells in milk, and to the use of lysozyme and other lysing enzyme preparations such as lysozyme G3x and lysosubtilin G10x in the manufacture of a feed composition.

The quality and composition of milk depends on many factors: feed quality and feeding technique, milking, cow breed, physiological status of animals, etc. Somatic cell count (SLK) is one of the main indicators of cow's milk quality. Somatic cells are always present in milk. When the SLK exceeds 300,000 cells / m, the quality of milk decreases. SLK greater than 300,000 cells / ml indicates that the cow's udder is irritated or unhealthy. According to 2000 In Lithuania, SLK must not exceed 400,000 cells / ml in the highest quality milk, up to 500,000 cells / ml in the first grade milk and 700,000 cells / ml in the second grade milk. SLK milk for human consumption must not exceed 300,000 cells / ml according to EU requirements in recent years.

From another perspective, SLK is an indicator of the immune status of cows. When the SLK in milk exceeds 300,000 cells / ml, it can be argued that the cow's immune system is unable to withstand external influences / infection. Therefore, increasing cow's resistance to environmental factors is also one of the possible ways to reduce SLK in milk and improve milk quality. For this purpose, various materials may be used which are administered to the animal in the same feed. These could be micronutrients and / or vitamins, which have a clear positive effect on the immune system, but other substances can be used. For example, feed additives containing Chinese tea powder and / or extract as the active ingredient are used to reduce SLK milk (JP 7 075 506); powder of extract of plant Quillaja saponaria (EP 1 219 178). The effectiveness of these techniques is not great.

We suggest using enzyme preparations as feed additives. Enzymes are known to be used as feed additives for: feed preservation (carbohydrate-degrading enzymes) (EP 0 346 909); to increase milk secretion and non-fat solids (cellulase, xylanase, dextranase, amylase, pectinase, protease) (US 4,144,354). EP 1 043 028 A1 describes the use of phytase for the treatment of mastitis as a cause of somatic cells in milk.

However, none of the enzymes mentioned herein has a significant effect on the immune system of animals.

We suggest the use of enzymes that can significantly increase the resistance of cows and at the same time have a significant effect on reducing SLK in milk and improving milk quality. These could be lysing enzymes, such as lysozyme, which release immune stimulants such as muropeptides, lipopolysaccharides, β-glucans, and the like by breaking down the microbial cell walls of the gastrointestinal tract.

The present invention provides for the use of lysozyme G3x and lysosubtilin G10x as a source of lysing enzymes. These are proteinaceous preparations and are therefore digested like other proteins after oral administration and fulfillment of their function. They are non-toxic, do not accumulate in the body, have no side effects, and do not form micro-organisms resistant to antimicrobial agents.

Lysozyme G3x is an enzyme preparation obtained by drying the culture fluid of deep-grown Bacillus subtilis bacteria in a spray dryer. It is a light gray, water-soluble powder. The lysozyme activity of the preparation is 300 ± 30 units / g.

Lysosubtilin G10x is a complex enzyme preparation obtained by precipitation of acetone in the culture fluid filtrate of deep-grown bacteria Bacillus subtilis. It is a fine, light gray or beige water soluble powder. The lysing activity of the preparation shall be not less than 1000000 units / g. It also contains amylolytic and proteolytic enzymes.

According to the present invention 100 to 500 mg / kg of animal weight, preferably 200 mg / kg of lysozyme G3x, is added to conventional cow feed and is administered once daily. Cows are fed this mixture for at least 5-30 days.

According to another embodiment of the invention, 10 to 50 mg / kg animal weight, preferably 20 mg / kg lysosubtilin G10x, is added to conventional cows feed and is administered once daily. Cows are fed this mixture for at least 5-30 days.

In another embodiment of the invention, 200 mg / kg lysozyme G3x and 20 mg / kg lysosubtilin G10x are added to conventional cow feeds and are administered once daily. Cows are fed this mixture for at least 5-30 days.

conventional cow feed is one that is well known to those skilled in the art. It can be a diet consisting of hay, silage, hay, straw, saladine, sugar beet, compound feed, mineral vitamin supplements, licking salt. During the grazing period, the cow's diet can consist of pasture grass, saladin, compound feed, licking salt.

Dairy tests are carried out using methods known in the art. The SLK is set individually for each cow using, for example, a Somascope instrument. The positive effect of a feed additive according to the present invention in reducing SLK in milk is evaluated as a percentage of the post-test SLK relative to the starting amount.

Using the proposed feed composition for at least 10 days reduces SLK in cow's milk by more than three times, and at the same time significantly improves milk quality.

The invention is further illustrated by the following examples.

Example 1.

Four groups of Lithuanian Black and White cows were used for the test, five cows per group. Cows are kept, fed and milked as usual, except that three cows are given lysosubtilin G10x for a period of ten days in addition to feed once a day. A control group of cows does not receive lysosubtilin G10x; the first treatment group receives lysosubtilin G10x 5 mg / kg animal weight; the second treatment group received lysosubtilin G10x 10 mg / kg animal weight; the third treatment group receives lysosubtilin G10x 20 mg / kg animal weight. The SCF in cow's milk is determined before and at the end of the test. The results are shown in the table.

Daily dose of lysosubtilin G10x (units / kg body weight) SLK in milk (cell / mI; average of 5 cows) SLK in milk after test, compared to baseline,% against test after the test O (control) 830,000 840,000 101.2 5 820,000 810,000 98.8 10th 800,000 660,000 82.5 20th 820,000 460,000 56.1

Example 2.

Four groups of Lithuanian Black and White cows were used for the test, five cows per group. Cows are kept, fed and milked as usual, except that three groups of cows are given lysozyme G3x together with feed once daily for a period of ten days. The control group of cows does not receive lysozyme G3x; the first treatment group received lysozyme G3x 50 mg / kg animal weight; the second treatment group received lysozyme G3x 100 mg / kg animal weight; the third treatment group receives lysozyme G3x 200 mg / kg animal weight. The SCF in cow's milk is determined before and at the end of the test. The results are shown in the table.

Dosage of lysozyme G3x (units / kg body weight) days of the animal SLK in milk (cells / ml; average of 5 cows) SLK in milk after test, compared to baseline,% before the test after the test O (control) 610,000 720,000 118.0 50 640,000 580,000 90.6 100 620,000 440,000 71.0 200 605,000 300,000 49.6

Example 3.

Three groups of Lithuanian Black and White cows were used for the test, nine cows per group. Cows are kept, fed and milked as usual, except that two groups of cows receive supplemental enzymatic preparations (with or without vitamins) for a period of fifteen days in addition to feed once daily. A control group of cows does not receive enzymes; the first treatment group received lysosubtilin G10x 20 mg / kg animal weight + lysozyme G3x 200 mg / kg animal weight; the second test group receives lysozyme G3x

200 mg / kg body weight + vitamins C, A, E. SLK in cows' milk is determined before and after the test. The results are shown in the table.

A group of cows SLK in milk (cells / ml; average of 9 cows) SLK in milk after test, compared to baseline,% before the test after the test control 740,000 1.270.000 171.6 pilot 1 760,000 309,000 40.7 pilot 2 730,000 205,000 28.1

Claims (9)

Definition Cow's feed composition comprising a conventional single dose of feed and an enzyme supplement, wherein the enzyme additive is a lysing enzyme. 2. The composition of claim 1, wherein the lysing enzyme preparations are lysozyme or other lysing enzyme preparations, such as lysozyme G3x, lysosubtilin G10x, or combinations thereof. 3. The composition of claims 1 and 2, wherein the enzyme additive comprises 100-500 mg / kg animal weight, preferably 200 mg / kg animal weight, of lysozyme G3x in a single feed dose. 4. The composition of claims 1 and 2, wherein the enzymatic additive comprises 10-50 mg / kg animal weight, preferably 20 mg / kg animal weight, of lysosubtilin G10x in a single feed dose. 5. The composition of claims 1 and 2, wherein the enzymatic additive comprises 200 mg / kg animal lysozyme G3x and 20 mg / kg animal lysosubtilin G10x in a single feed dose. 6. A composition according to any one of claims 1 to 5, wherein the composition is administered to cows once daily for at least 5-30 days, preferably for at least 10 days. 7. The composition of any one of claims 1-6, further comprising vitamins such as A, C, E, trace elements and calcium formulations. A composition according to claims 1-7 for reducing somatic cell content in cow's milk. Use of lysozyme and other lysing enzyme preparations, such as lysozyme G3x, lysosubtilin G10x, in the manufacture of a feed composition according to any one of claims 1-8.