LT4382B - The ointment for wound treatment - Google Patents

Abstract

Wound treatment ointment. Invention is being attributed to the domain of veterinary and may be used for treatments of hard healing and festering wounds both in veterinary and medical practice. In order to cut reduce treatment duration, speed-up wound reparative processes we propose an ointment, that embraces antimicrobe, anti inflammation and ointment basis comprising substances: cigerol, glucose, insulin, ascorbic acid, chymotrypsine, lanolin at a following mass percentage composition: cigerol (20,0-25,0); 40 per cent solution of glucose (10,0-15,0); crystalline insulin (2,0-5,0); 5 per cent ascorbic acid (5,0-10,0); crystalline chymotrypsine (1,0-3,0); anhydrous lanolin (40,0-50,0).

Description

The invention relates to the field of veterinary medicine and can be used in veterinary and medical practice, in particular in the treatment of difficult to heal, purulent wounds.

In recent years, many new ointments have been developed in both medical and veterinary practice, based on antibacterial agents, proteolytic lysing-type enzymes, which have immediate healing and tissue-stimulating properties. A prominent place in the treatment of wounds of various origins is AV Vyshnevski's balsamic ointment (MD Mashkovsky, Medications, M. Medicine, 1987, vol. 2, p. 414.), ointment "Salkoseril" (Solkoserig, prospectus of the company "Alkaloid-skopje"). Yugoslavia.), 'Truksol' (Iruksol 'prospectus of' Pūva 'firm. Zagreb, Yugoslavia. ^ MD Mashkovsky. Medications. M. Medicine. 1987, 2, p. 56) and an ointment for long-term wounds (Description of the invention according to RU patent No. 2027432, Cl, Int. Cl. A61K / 06.).

The disadvantage of these ointments is that they take from 1 to 2 to 3 months for treatment of putrid, long-lasting wounds.

The object of the invention is to reduce the duration of treatment of purulent, non-healing wounds and to increase the pharmacological activity of the ointment.

For this purpose, we offer the following components, lubricant, by weight:

Cigerol 20.0 - 25.0

40% glucose solution 10.0 -15.0

Crystalline Insulin 2.0 - 5.0

5% ascorbic acid 5.0 -10.0

Crystalline chymotrypsin 1.0 - 3.0

Anhydrous lanolin 40.0 - 50.0

Justification of lubricant components

Cigerol - 4,8 (+) - DL - 2-cyclohexyl-5,9-dimethyl-capric acid: fatty in consistency, has antimicrobial, anti-inflammatory and reparative properties. Used to treat externally granular trophic inflammatory wounds. It kills Gr + and Gr- bacteria, as well as some microflora, activates granulation and epithelial growth, and promotes wound healing.

Glucose is a crystalline powder, highly soluble in water. The solutions are sterilized at pH 3.0-4.0. When glucose is used as sugar, alkaline wound secretions are found to produce an acidic reaction. Bacteria do not multiply in an acidic environment. Increases wound secretion, hypertonic glucose solutions have bacteriostatic, drainage properties, help break down necrotic tissues, and do not traumatize tissue cells.

Insulin is a hormonal preparation. Water-soluble crystals regulate sugar content, accelerate the movement of substances across the cell membrane, stimulate the activity of enzymes inside the cell, and stimulate biosynthetic processes in the cell. In surgical practice, it is known that insulin wound dressing accelerates wound granulation and repair.

Chymotrypsin is a proteolytic enzyme. It hydrolyses not only proteins but also necrotic tissues, liquefies wound secretions, exudates, and acts anti-inflammatory.

Vitamin C - ascorbic acid. Its presence in the molecule of the diene group (-COH = COH-) gives the vitamin strong restorative properties. Participates in oxidation reduction process, carbohydrate uptake, increases blood coagulation, tissue regeneration. Involved in synthesis of procollagen and collagen, normalizes capillary permeability, has anti-inflammatory properties.

Lanolin binds ingredients well, is easily absorbed into skin and wound, indifferent, retains well.

We formulated an ointment from well-known formulations of pharmacology, which, when interacting with each other, form a new treatment with a complex effect on the wound: the ointment is easily absorbed, has dehydrative - drainage antibacterial action. It accelerates tissue repair processes, promotes growth of the covering epithelium, tissue collagenase, and helps wound healing.

An example. This ointment has been used for two years in Kaunas Sports School to treat wounds of sports horses: traumatic origin, after various injuries, mainly infected abscesses.

Twenty-three horses were treated during this period. The nature of the wounds and the duration of treatment are given in Table no. 1. The wounds of twelve horses (control group) were treated with a variety of medications, most notably abscess, by A. V. Vyshnevski, Iruksol ointment, antibiotic and stranyl amide ointment, iodoform. The nature of the wounds and the duration of treatment are given in Table no. 2.

The course of treatment. Initially, the surface of the wound is wiped dry with sterile wipes, and the hair is wiped with iodine solution around the wound. Gently use sterile gauze pads to remove dirt and pus from the wound surface. If the wound is heavily contaminated, it is rinsed with 2-3% hydrogen peroxide or other disinfectant solutions. After cleaning, the condition, anatomical position, width, length, depth, etc. of the damaged tissue are determined. t. Deeper pockets in the wound are opened, allowing the wound exudate to drain. The wound so prepared is covered with a 2 mm layer of our recommended grease and covered with sterile gauze wipes. The bandage is changed twice a day and the necrotized tissues are mechanically removed using as few solutions as possible. Grease again. When the wounds become clean, the granulation tissue develops, we stop using the ointment. For further treatment we use dehydrating, bacteriocidal preparations. By treating the wounds of the animals with the aforementioned ointment, they already had pain, swelling around the wound, temperature, pulse, and breathing at the end of the first day.

The wound quickly clears from pus, and the granulation becomes reddish. Wound epithelialization is markedly activated and granulation continues for three days. After 3-4 days the wound is completely healed.

The treatment is open. The advantage of this method is that when the wound is untied, the moisture evaporates faster. Oil and air exposed wound exudate declined rapidly and a crust formed on the wound surface. In this way, we achieved a mean wound healing time of 8.445 ± 0.45 days (see Table j, Table 1).

The microbiological and cytological composition of the wound exudate was investigated at the start of treatment, 48 hours later. is after 96 or.

In the first days, 24.67% of mononuclear cells, 35.83% of polynuclear cells and 16.5% of intact leukocytes were found from purulent ulcerative wounds. After 48 hours, the ointment showed a 3.87% reduction in mononuclear, 9.87% in polynuclear (P <0.001), and an increase in normal leukocytes of 9.84% (P <0.01). 7.89% (P <0.05) of strongly expressed phagocytosis was detected. A second application of ointment resulted in a 8.99% reduction in mononuclear (P <0.001). After 96 or. we found 15.87% (P <0.001) of treatment cells with strongly expressed phagocytosis.

During the examination of the wound exudate during the first days of treatment, bacteriological contamination was detected in all samples. Mixed relatively pathogenic microflora (25%) was mainly isolated. Staphylococci were isolated in 16.66%, proteus in 33.33%, intestinal rod in 8.33% and blue rot in -16.66%.

Bacterial contamination was virtually unchanged after initial application of the ointment (with 48 h intervals), with more mixed, relatively pathogenic, microflora detected in 16.6% of the samples.

96 hours later. after treatment, staphylococci were not isolated from the wound exudate, and proteus and intestinal sticks were reduced, as well as relatively pathogenic microflora. 59% of the samples were sterile.

⁴ LT 4382 B

Clinical observations, cytological and bacteriological studies have shown that the ointment has dehydrative, drainage, reparative properties, antibacterial activity. The median duration of treatment is 8.445 days with 17 applications, whereas conventional wound treatment for such wounds takes 25.67 ± 1.58 days (see Table 2).

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Claims (1)

Definition Wound ointment combining antimicrobial, anti-inflammatory and basic substances, characterized in that it contains cigerol, glucose, insulin, ascorbic acid, chymotrypsin, lanolin, in the composition of the above components, by weight: cigerol 20.0 - 25.0 40% glucose solution 10.0 -15.0 crystalline insulin 2.0 - 5.0 5% ascorbic acid 5.0 to 10.0 crystalline chymotrypsin 1.0 - 3.0 anhydrous lanolin 40.0 - 50.0.