LT2020027A - Ląstelių mikroporatorius - Google Patents

Ląstelių mikroporatorius

Info

Publication number
LT2020027A
LT2020027A LT2020027A LT2020027A LT2020027A LT 2020027 A LT2020027 A LT 2020027A LT 2020027 A LT2020027 A LT 2020027A LT 2020027 A LT2020027 A LT 2020027A LT 2020027 A LT2020027 A LT 2020027A
Authority
LT
Lithuania
Prior art keywords
rollers
cell
microporator
concave
cells
Prior art date
Application number
LT2020027A
Other languages
English (en)
Inventor
Linas JONUŠAUSKAS
Roaldas GADONAS
Original Assignee
Uab "Femtika"
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Uab "Femtika" filed Critical Uab "Femtika"
Priority to LT2020027A priority Critical patent/LT2020027A/lt
Priority to PCT/IB2020/059497 priority patent/WO2022013607A1/en
Priority to US17/375,851 priority patent/US20220017924A1/en
Publication of LT2020027A publication Critical patent/LT2020027A/lt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M35/00—Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for electroporation or cell fusion
    • C12M35/04—Mechanical means, e.g. sonic waves, stretching forces, pressure or shear stimuli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/02—Form or structure of the vessel
    • C12M23/16—Microfluidic devices; Capillary tubes

Landscapes

  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Sustainable Development (AREA)
  • Plant Pathology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Dispersion Chemistry (AREA)
  • Clinical Laboratory Science (AREA)
  • Mechanical Engineering (AREA)
  • Cell Biology (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

Išradimas priklauso biomedicinos sričiai, konkrečiau - ląstelių transfekavimo įrenginiams. Ląstelių mikroporatorius, susideda iš skysčio mikrokanalo (1) ir jame patalpintų mechaninio poveikio elementų, kurie padaryti kaip du besisukantys velenėliai (2, 2a, 2b) (sinonimas „voleliai“) ir sumontuoti mikrokanalo (1) nišose (la, lb) vienas nuo kito atstumu mažesniu negu apdorojamų ląstelių dydis, vienas arba abu velenėliai (2, 2a, 2b) padaryti su krumpliais (3) arba spygliais (5) (adatom), velenėliai yra pasyvūs, sukami pratekančio skysčio ir kontaktuoja su ląstelėmis vienu šonu. Siūlomi įvairūs velenėlių formos variantai: cilindriniai, išgaubtas- įgaubtas (pora), įgaubti, laiptuoti cilindriniai. Siūlomi velenėlių montavimo variantai: velenėliai galuose turi įdubas, o skysčio mikrokanale padarytos iškyšos (trumpos ašelės) ir atvirkščiai - velenėliai galuose turi įdubas, o skysčio mikrokanale padarytos iškyšos (trumpos ašelės), kurios tarnauja kaip slydimo guoliai, arba velenėliai sumontuoti ant ašių.
LT2020027A 2020-07-14 2020-07-14 Ląstelių mikroporatorius LT2020027A (lt)

Priority Applications (3)

Application Number Priority Date Filing Date Title
LT2020027A LT2020027A (lt) 2020-07-14 2020-07-14 Ląstelių mikroporatorius
PCT/IB2020/059497 WO2022013607A1 (en) 2020-07-14 2020-10-09 Cell microporator
US17/375,851 US20220017924A1 (en) 2020-07-14 2021-07-14 Cell Microporator

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
LT2020027A LT2020027A (lt) 2020-07-14 2020-07-14 Ląstelių mikroporatorius

Publications (1)

Publication Number Publication Date
LT2020027A true LT2020027A (lt) 2022-01-25

Family

ID=72964757

Family Applications (1)

Application Number Title Priority Date Filing Date
LT2020027A LT2020027A (lt) 2020-07-14 2020-07-14 Ląstelių mikroporatorius

Country Status (3)

Country Link
US (1) US20220017924A1 (lt)
LT (1) LT2020027A (lt)
WO (1) WO2022013607A1 (lt)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN115254214B (zh) * 2022-06-29 2023-07-25 中国科学院精密测量科学与技术创新研究院 一种微流控通道、微流控芯片和生化分子递送方法

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1124939B1 (en) 1998-10-08 2003-04-23 AstraZeneca AB Microfabricated cell injector
KR100421359B1 (ko) * 2001-07-24 2004-03-06 엘지전자 주식회사 탄성 재질의 기판 내에서 유체를 이송하는 방법 및 이를위한 장치
US20040126254A1 (en) * 2002-10-31 2004-07-01 Chen Ching Jen Surface micromachined mechanical micropumps and fluid shear mixing, lysing, and separation microsystems
US9017991B2 (en) 2009-03-13 2015-04-28 Tufts University Methods tip assemblies and kits for introducing material into cells
WO2018089497A1 (en) 2016-11-08 2018-05-17 Georgia Tech Research Corporation Methods for convectively-driven intracellular delivery
EP3556845A1 (en) 2018-04-20 2019-10-23 Cellix Limited A method and device for transfecting cells

Also Published As

Publication number Publication date
WO2022013607A1 (en) 2022-01-20
US20220017924A1 (en) 2022-01-20

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Legal Events

Date Code Title Description
BB1A Patent application published

Effective date: 20220125

FD9A Withdrawn applications