LT2015019A - Viengrandės rnr analizė - Google Patents

Viengrandės rnr analizė

Info

Publication number
LT2015019A
LT2015019A LT2015019A LT2015019A LT2015019A LT 2015019 A LT2015019 A LT 2015019A LT 2015019 A LT2015019 A LT 2015019A LT 2015019 A LT2015019 A LT 2015019A LT 2015019 A LT2015019 A LT 2015019A
Authority
LT
Lithuania
Prior art keywords
ssrna
analysis
stranded rna
factor
methyltransferase
Prior art date
Application number
LT2015019A
Other languages
English (en)
Other versions
LT6341B (lt
Inventor
Saulius Klimašauskas
Giedrius Vilkaitis
Milda Mickutė
Original Assignee
Vilniaus Universitetas
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Vilniaus Universitetas filed Critical Vilniaus Universitetas
Priority to LT2015019A priority Critical patent/LT6341B/lt
Priority to EP16720576.4A priority patent/EP3271478B1/en
Priority to PCT/LT2016/000002 priority patent/WO2016148556A1/en
Priority to US15/559,689 priority patent/US11008605B2/en
Publication of LT2015019A publication Critical patent/LT2015019A/lt
Publication of LT6341B publication Critical patent/LT6341B/lt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2521/00—Reaction characterised by the enzymatic activity
    • C12Q2521/10—Nucleotidyl transfering
    • C12Q2521/125—Methyl transferase, i.e. methylase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/137—Metal/ion, e.g. metal label

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Išradime pateiktas vgRNR 3’ galo modifikacijos metodas, kuris apima grandinės sujungimą su vgRNR 2'-O-metiltransferaze, esant kofaktoriui, tokiomis sąlygomis, kurios leidžia pernešti dalį kofaktoriaus, naudojant vgRNR 2'-O-metiltransferazę, ant vgRNR 3' galo. Tokiu būdu gaunama modifikuota vgRNR. Šiame metode naudojama vgRNR, kurios 3' galinis nukleotidas turi 2'-OH grupę, o dalį pernešamo kofaktoriaus sudaro reporterinė arba funkcinė grupė.
LT2015019A 2015-03-19 2015-03-19 Viengrandės rnr analizė LT6341B (lt)

Priority Applications (4)

Application Number Priority Date Filing Date Title
LT2015019A LT6341B (lt) 2015-03-19 2015-03-19 Viengrandės rnr analizė
EP16720576.4A EP3271478B1 (en) 2015-03-19 2016-03-18 Analysis of single-stranded rna
PCT/LT2016/000002 WO2016148556A1 (en) 2015-03-19 2016-03-18 Analysis of single-stranded rna
US15/559,689 US11008605B2 (en) 2015-03-19 2016-03-18 Analysis of single-stranded RNA

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
LT2015019A LT6341B (lt) 2015-03-19 2015-03-19 Viengrandės rnr analizė

Publications (2)

Publication Number Publication Date
LT2015019A true LT2015019A (lt) 2016-09-26
LT6341B LT6341B (lt) 2016-12-27

Family

ID=55911025

Family Applications (1)

Application Number Title Priority Date Filing Date
LT2015019A LT6341B (lt) 2015-03-19 2015-03-19 Viengrandės rnr analizė

Country Status (4)

Country Link
US (1) US11008605B2 (lt)
EP (1) EP3271478B1 (lt)
LT (1) LT6341B (lt)
WO (1) WO2016148556A1 (lt)

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1712557A1 (en) * 2005-04-14 2006-10-18 RWTH Aachen New s-adenosyl-L-methionine analogues with extended activated groups for transfer by methyltransferases
GB201210756D0 (en) * 2012-06-15 2012-08-01 Univ Vilnius Analysis of small RNA

Also Published As

Publication number Publication date
EP3271478B1 (en) 2020-05-06
US11008605B2 (en) 2021-05-18
EP3271478A1 (en) 2018-01-24
US20180251814A1 (en) 2018-09-06
LT6341B (lt) 2016-12-27
WO2016148556A1 (en) 2016-09-22

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Legal Events

Date Code Title Description
BB1A Patent application published

Effective date: 20160926

FG9A Patent granted

Effective date: 20161227

MM9A Lapsed patents

Effective date: 20170319