LT2002133A - Chaperons DnaK, DnaJ and GrpE from Meiothermus ruber having increased thermal stability and system and process for refolding proteins in vitro - Google Patents
Chaperons DnaK, DnaJ and GrpE from Meiothermus ruber having increased thermal stability and system and process for refolding proteins in vitroInfo
- Publication number
- LT2002133A LT2002133A LT2002133A LT2002133A LT2002133A LT 2002133 A LT2002133 A LT 2002133A LT 2002133 A LT2002133 A LT 2002133A LT 2002133 A LT2002133 A LT 2002133A LT 2002133 A LT2002133 A LT 2002133A
- Authority
- LT
- Lithuania
- Prior art keywords
- chaperons
- refolding
- grpe
- dnak
- dnaj
- Prior art date
Links
Landscapes
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
- Enzymes And Modification Thereof (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The present invention claims protein refolding system which includes thermophilic chaperons cloned from mezophilic microorganism Meiothermus rubber. The chaperons mentioned above are characterized by increased thermal stability and refolding activity compared to chaperons known previously. The refolding system claimed (the refolding buffer) consists of denaturated protein, neorganic salts, the mixture of three purified thermophilic chaperons (DnaK, DnaJ and GrpE) and adenosintriphosphate. The invention belongs to biotechnology and may be useful in restoring in vitro the active structure of native and recombinant proteins in the process of their preparation.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
LT2002133A LT5053B (en) | 2002-12-24 | 2002-12-24 | Chaperons DnaK, DnaJ and GrpE from Meiothermus ruber having increased thermal stability and system and process for refolding proteins in vitro |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
LT2002133A LT5053B (en) | 2002-12-24 | 2002-12-24 | Chaperons DnaK, DnaJ and GrpE from Meiothermus ruber having increased thermal stability and system and process for refolding proteins in vitro |
Publications (2)
Publication Number | Publication Date |
---|---|
LT2002133A true LT2002133A (en) | 2003-07-25 |
LT5053B LT5053B (en) | 2003-09-25 |
Family
ID=27730946
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
LT2002133A LT5053B (en) | 2002-12-24 | 2002-12-24 | Chaperons DnaK, DnaJ and GrpE from Meiothermus ruber having increased thermal stability and system and process for refolding proteins in vitro |
Country Status (1)
Country | Link |
---|---|
LT (1) | LT5053B (en) |
Families Citing this family (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US7939711B2 (en) | 2006-09-11 | 2011-05-10 | The Chinese University Of Hong Kong | Abiotic stress tolerance conferred by J-domain containing proteins |
Family Cites Families (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP3344618B2 (en) | 1997-06-20 | 2002-11-11 | 株式会社エイチ・エス・ピー研究所 | Chaperone expression plasmid |
WO1999005163A1 (en) | 1997-07-24 | 1999-02-04 | Medical Research Council | Refolding method using a foldase and a chaperone |
JP4249832B2 (en) | 1998-12-28 | 2009-04-08 | タカラバイオ株式会社 | Trigger factor expression plasmid |
EP1149909A1 (en) | 2000-04-28 | 2001-10-31 | Boehringer Mannheim Gmbh | Methods for regulating protein conformation using molecular chaperones |
-
2002
- 2002-12-24 LT LT2002133A patent/LT5053B/en not_active IP Right Cessation
Also Published As
Publication number | Publication date |
---|---|
LT5053B (en) | 2003-09-25 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
GB2438760A (en) | Methods for expression and purification of recombinant human growth hormone | |
BRPI0412799A (en) | immunogen, composition, nucleic acid, recombinant cell, methods for preparing a polypeptide, for inducing a protective immune response in a patient and for inducing an anamnesic response in a patient, and optimized yeast nucleic acid sequence | |
BRPI0516572A (en) | methods and compositions for improving the production of recombinant proteins | |
EP1422237A3 (en) | Methods for the recombinant production of antifusogenic peptides | |
PE20110310A1 (en) | AXMI-115, AXMI-113, AXMI-005, AXMI-163 AND AXMI-184: INSECTICIDE PROTEINS | |
WO2003057897A3 (en) | Recombinant protein expression | |
BRPI0410562A (en) | recombinant molecule and vehicle for expression, release and purification of target polypeptides | |
PE20231439A1 (en) | HMPV F PROTEINS STABILIZED BY PREFUSION | |
SG163592A1 (en) | Methods and compositions for increasing longevity and protein yield from a cell culture | |
BR0313083A (en) | modified adamts4 molecules, polynucleotide, vector, processes for production, pharmaceutical composition as well as use thereof | |
Aghaabdollahian et al. | Molecular cloning of Reteplase and its expression in E. coli using tac promoter | |
DE602004024122D1 (en) | PREVENTING INSTALLATION OF NON-TANDARD AMINO ACIDS IN PROTEIN | |
LT2002133A (en) | Chaperons DnaK, DnaJ and GrpE from Meiothermus ruber having increased thermal stability and system and process for refolding proteins in vitro | |
KR20210089699A (en) | E. coli strains with oxidative cytoplasm | |
EP1728859A4 (en) | Sequence capable of accelerating gene expression at moderately low temperature | |
WO2006097945A3 (en) | A method for achieving high-level expression of recombinant human interleukin-2 upon destabilization of the rna secondary structure | |
BR112022026248A2 (en) | CYTOKINE CONJUGATES | |
Shiguang et al. | Purification and properties of a new L-sorbose dehydrogenase accelerative protein from Bacillus megaterium bred by ion-beam implantation | |
Tamai et al. | High-level expression of his-tagged clostridial collagenase in Clostridium perfringens | |
PL423299A1 (en) | Method for cleanup of the environment contaminated by sulfur esters, new bacterial strains, new proteins, nucleic acid construct, vector, host cell, method for producing bacterial strains and new proteins, a composition of their application and method for the environment bioremediation | |
JP2018143142A (en) | Polypeptide having angiotensin-converting enzyme 2 activity, gene encoding polypeptide, expression plasmid containing gene, transformant transformed with expression plasmid and method for producing enzyme | |
DK1472347T3 (en) | Improved protein expression in Bacillus Subtilis | |
EP3221449B1 (en) | Process for refolding recombinant chymotrypsin | |
White | Protein expression in corynebacterium glutamicum | |
Zhu et al. | High-yield soluble expression of recombinant human interleukin-1β in Escherichia coli |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
MM9A | Lapsed patents |
Effective date: 20041224 |