LT2002133A - Chaperons DnaK, DnaJ and GrpE from Meiothermus ruber having increased thermal stability and system and process for refolding proteins in vitro - Google Patents

Chaperons DnaK, DnaJ and GrpE from Meiothermus ruber having increased thermal stability and system and process for refolding proteins in vitro

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Publication number
LT2002133A
LT2002133A LT2002133A LT2002133A LT2002133A LT 2002133 A LT2002133 A LT 2002133A LT 2002133 A LT2002133 A LT 2002133A LT 2002133 A LT2002133 A LT 2002133A LT 2002133 A LT2002133 A LT 2002133A
Authority
LT
Lithuania
Prior art keywords
chaperons
refolding
grpe
dnak
dnaj
Prior art date
Application number
LT2002133A
Other languages
Lithuanian (lt)
Other versions
LT5053B (en
Inventor
Milda PLEČKAITYTĖ
Edita MIŠTINIENĖ
Vilma MICHAILOVIENĖ
Henrikas PESLIAKAS
Gintautas ŽVIRBLIS
Original Assignee
Biotechnologijos Institutas
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Biotechnologijos Institutas filed Critical Biotechnologijos Institutas
Priority to LT2002133A priority Critical patent/LT5053B/en
Publication of LT2002133A publication Critical patent/LT2002133A/en
Publication of LT5053B publication Critical patent/LT5053B/en

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  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

The present invention claims protein refolding system which includes thermophilic chaperons cloned from mezophilic microorganism Meiothermus rubber. The chaperons mentioned above are characterized by increased thermal stability and refolding activity compared to chaperons known previously. The refolding system claimed (the refolding buffer) consists of denaturated protein, neorganic salts, the mixture of three purified thermophilic chaperons (DnaK, DnaJ and GrpE) and adenosintriphosphate. The invention belongs to biotechnology and may be useful in restoring in vitro the active structure of native and recombinant proteins in the process of their preparation.
LT2002133A 2002-12-24 2002-12-24 Chaperons DnaK, DnaJ and GrpE from Meiothermus ruber having increased thermal stability and system and process for refolding proteins in vitro LT5053B (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
LT2002133A LT5053B (en) 2002-12-24 2002-12-24 Chaperons DnaK, DnaJ and GrpE from Meiothermus ruber having increased thermal stability and system and process for refolding proteins in vitro

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
LT2002133A LT5053B (en) 2002-12-24 2002-12-24 Chaperons DnaK, DnaJ and GrpE from Meiothermus ruber having increased thermal stability and system and process for refolding proteins in vitro

Publications (2)

Publication Number Publication Date
LT2002133A true LT2002133A (en) 2003-07-25
LT5053B LT5053B (en) 2003-09-25

Family

ID=27730946

Family Applications (1)

Application Number Title Priority Date Filing Date
LT2002133A LT5053B (en) 2002-12-24 2002-12-24 Chaperons DnaK, DnaJ and GrpE from Meiothermus ruber having increased thermal stability and system and process for refolding proteins in vitro

Country Status (1)

Country Link
LT (1) LT5053B (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7939711B2 (en) 2006-09-11 2011-05-10 The Chinese University Of Hong Kong Abiotic stress tolerance conferred by J-domain containing proteins

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP3344618B2 (en) 1997-06-20 2002-11-11 株式会社エイチ・エス・ピー研究所 Chaperone expression plasmid
WO1999005163A1 (en) 1997-07-24 1999-02-04 Medical Research Council Refolding method using a foldase and a chaperone
JP4249832B2 (en) 1998-12-28 2009-04-08 タカラバイオ株式会社 Trigger factor expression plasmid
EP1149909A1 (en) 2000-04-28 2001-10-31 Boehringer Mannheim Gmbh Methods for regulating protein conformation using molecular chaperones

Also Published As

Publication number Publication date
LT5053B (en) 2003-09-25

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Effective date: 20041224