KR980002247A - New strain Bacillus rickeniformis and novel exopeptidase produced therefrom - Google Patents

New strain Bacillus rickeniformis and novel exopeptidase produced therefrom Download PDF

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KR980002247A
KR980002247A KR1019960019826A KR19960019826A KR980002247A KR 980002247 A KR980002247 A KR 980002247A KR 1019960019826 A KR1019960019826 A KR 1019960019826A KR 19960019826 A KR19960019826 A KR 19960019826A KR 980002247 A KR980002247 A KR 980002247A
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South Korea
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exopeptidase
novel
new strain
strain bacillus
produced therefrom
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KR1019960019826A
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Korean (ko)
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KR0176741B1 (en
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오태광
박미자
김형권
배경숙
최인재
남희섭
신재익
이형재
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박원훈
한국과학기술연구원
이상윤
주식회사 농 심
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Priority to KR1019960019826A priority Critical patent/KR0176741B1/en
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23C—DAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; MAKING OR TREATMENT THEREOF
    • A23C19/00—Cheese; Cheese preparations; Making thereof
    • A23C19/02—Making cheese curd
    • A23C19/032—Making cheese curd characterised by the use of specific microorganisms, or enzymes of microbial origin
    • A23C19/0328—Enzymes other than milk clotting enzymes, e.g. lipase, beta-galactosidase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C12N1/205—Bacterial isolates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/485—Exopeptidases (3.4.11-3.4.19)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/07—Bacillus
    • C12R2001/10—Bacillus licheniformis

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Molecular Biology (AREA)
  • Food Science & Technology (AREA)
  • Polymers & Plastics (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

본 발명은 신균주 바실러스 리케니포르미스(Bacillus licheniformis) NS115(KCTC 0240BP)와 이로부터 생산되는 신규 엑소펩티다아제(Exopeptidase)에 관한 것으로서, 더욱 상세하게는 치즈숙성과정을 가속화 시킬 수 있으며, 우유단백질이 갖고 있는 항원물질을 제거할 뿐 아니라 단백질을 분해하여 향미성분을 제조하여 독특한 풍미를 내도록 하는 효소인 엑소펩티다아제를 생산하는 신균주 바실러스 리케니포르미스(Bacillus licheniformis) NS115(KCTC 0240BP)와 이로부터 생산되는 신규 엑소펩티다아제(Exopeptidase)에 관한 것이다.The present invention relates to the new strain Bacillus licheniformis NS115 (KCTC 0240BP) and a novel exopeptidase (Exopeptidase) produced therefrom, and in more detail can accelerate the cheese ripening process, milk protein Bacillus licheniformis NS115 (KCTC 0240BP), which produces exopeptidase, an enzyme that not only removes antigenic substances but also breaks down proteins to produce flavor components and produces unique flavors To a novel Exopeptidase.

Description

신균주 바실러스 리케니포르미스와 이로부터 생산되는 신규 엑소펩티다아제New strain Bacillus rickeniformis and novel exopeptidase produced therefrom

본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음Since this is an open matter, no full text was included.

제1도는 본 발명의 신균주 바실러스 리케니포르미스(Bacillus licheniformis) NS115의 전자현미경 사진(×20,000)이다.1 is an electron micrograph (× 20,000) of the new strain Bacillus licheniformis NS115 of the present invention.

제2도는 본 발명 실시예 2에 따른 신규 엑소펩티다아제와 표준단백질과의 SDS 전기 영동사진을 나타낸 것이다.Figure 2 shows the SDS electrophoresis picture of the novel exopeptidase and the standard protein according to Example 2 of the present invention.

제3도는 본 발명 실시예 2에 따른 신규 엑소펩티다아제와 표준단백질과의 네이티브(Native)전기영동사진을 나타낸 것이고, 제4도는 본 발명 실험예 2에 따른 신규 엑소펩티다아제의 등전점을 측정한 것이다.Figure 3 shows the native electrophoresis picture of the novel exopeptidase and the standard protein according to Example 2 of the present invention, Figure 4 is to measure the isoelectric point of the novel exopeptidase according to Experimental Example 2 of the present invention.

Claims (5)

신균주 바실러스 리케니포르미스(Bacillus licheniformis) NS115(KCTC 0240BP).New strain Bacillus licheniformis NS115 (KCTC 0240BP). 제1항의 미생물을 단백질 분해과정 중에 첨가하는 사용하는 방법.A method of adding the microorganism of claim 1 during proteolysis. 제2항에 있어서, 상기 단백질 분해 과정은 치즈숙성과정인 것임을 특징으로 하는 방법.The method of claim 2, wherein the protein degradation process is characterized in that the cheese ripening process. 제1항의 미생물이 생산하는 최적온도가 55℃이고, 최적 pH가 8.0이며, 분자량이 42 kDa과 22 kDa이고, 등전점이 5.2이며, N-말단아미노산 서열이 〔서열표〕의 서열번호 1과 2로 표시되는 신규 엑소펩티다아제.The optimum temperature produced by the microorganism of claim 1 is 55 ° C, the optimum pH is 8.0, the molecular weight is 42 kDa and 22 kDa, the isoelectric point is 5.2, and the N-terminal amino acid sequence is SEQ ID NOs: 1 and 2 of SEQ ID NO: New exopeptidase represented by. 제4항의 엑소펩티다아제를 맛개선을 위한 유효성분으로 하는 치즈 첨가제.A cheese additive comprising the exopeptidase of claim 4 as an active ingredient for taste improvement. ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.※ Note: The disclosure is based on the initial application.
KR1019960019826A 1996-06-04 1996-06-04 New strain Bacillus licheniformis NS115 and novel exopeptidase produced therefrom KR0176741B1 (en)

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KR1019960019826A KR0176741B1 (en) 1996-06-04 1996-06-04 New strain Bacillus licheniformis NS115 and novel exopeptidase produced therefrom

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Application Number Priority Date Filing Date Title
KR1019960019826A KR0176741B1 (en) 1996-06-04 1996-06-04 New strain Bacillus licheniformis NS115 and novel exopeptidase produced therefrom

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KR980002247A true KR980002247A (en) 1998-03-30
KR0176741B1 KR0176741B1 (en) 1999-04-01

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR100747701B1 (en) * 2006-03-24 2007-08-08 영남대학교 산학협력단 11 Antagonistic soil bacterium Bacillus Licheniformis K11 and biological control and plant growth promotion using the same

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR100747701B1 (en) * 2006-03-24 2007-08-08 영남대학교 산학협력단 11 Antagonistic soil bacterium Bacillus Licheniformis K11 and biological control and plant growth promotion using the same

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