KR970705752A - Inspection method and inspection kit - Google Patents

Inspection method and inspection kit

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Publication number
KR970705752A
KR970705752A KR1019970701223A KR19970701223A KR970705752A KR 970705752 A KR970705752 A KR 970705752A KR 1019970701223 A KR1019970701223 A KR 1019970701223A KR 19970701223 A KR19970701223 A KR 19970701223A KR 970705752 A KR970705752 A KR 970705752A
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KR
South Korea
Prior art keywords
trapping material
region
binding
gelatin
kit
Prior art date
Application number
KR1019970701223A
Other languages
Korean (ko)
Other versions
KR100411406B1 (en
Inventor
토루 요시무라
Original Assignee
에이치. 토마스 왓킨스
다이나보트 가부시끼가이샤
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Application filed by 에이치. 토마스 왓킨스, 다이나보트 가부시끼가이샤 filed Critical 에이치. 토마스 왓킨스
Publication of KR970705752A publication Critical patent/KR970705752A/en
Application granted granted Critical
Publication of KR100411406B1 publication Critical patent/KR100411406B1/en

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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54366—Apparatus specially adapted for solid-phase testing
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals

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  • Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

분석 물질과 특이적으로 결합 가능한 트래핑 물질(2)이 크로마토그래피 캐리어에 고정되어 있는 위치에 결합 영역을 가지며 분석 물질과 특이하게 경쟁적인 트래킹 물질(2)에 결합 가능한 트래핑 물질(1) 및 표지 물질로 구성된 추적자가 시료 전개가 실행될 수 있도록 시료 첨가 전 또는 첨가 시에 결합 영역의 상류 구역에 배치된 크로마토그래피 스트립을 사용하는 검사 방법에서, 젤라틴은 시료 첨가 시에 결합 영역의 상류 구역에 첨가되거나 또는 시료 첨가 전에 미리 상류 구역에 배치된다.A trapping material (1) capable of binding to a tracking material (2) having a binding region at a position at which the trapping material (2) capable of specifically binding to the analyte is immobilized on the chromatography carrier, Gelatine is added to the upstream zone of the binding region at the time of sample addition, or in a test method using chromatographic strips placed in the upstream zone of the binding zone before or during addition of the sample so that the sample development can be carried out, Is placed in the upstream zone in advance of sample addition.

Description

검사 방법 및 검사 용 키트Inspection method and inspection kit

본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음Since this is a trivial issue, I did not include the contents of the text.

제1도는 크로마토그래피 스트립을 도시하는 도면이다. 제2도는 본 발명의 검사 방법에 의해 수행된 검사 결과(실시예 2)를 도시하는 그래프이다. 제3도는 본 발명의 검사 방법에 의해 수행된 검사 결과(실시예 3)를 도시하는 그래프이다.Figure 1 is a diagram showing a chromatographic strip. FIG. 2 is a graph showing the inspection result (Example 2) performed by the inspection method of the present invention. FIG. 3 is a graph showing the inspection result (Example 3) performed by the inspection method of the present invention.

Claims (10)

크로마토그래피 케리어가, 분석 물질과 결합 가능한 트래킹 물질(2)이 분석 물질 및 트래핑 물질(2)과 결합 가능한 트래핑 물질(3)을 통하여 직접 또는 간접적으로 고정되거나 또는 분석 물질 또는 분석 물질과 경쟁적인 트래핑 물질(2)과 특이적으로 결합하는 트래핑 물질(1)에 특이적으로 결합 가능한 트래핑 물질(4)이 트래핑 물질(2)이 고정된 영역의 하류 구역에 고정된 결합 영역을 하나 이상 갖고 또 트래핑 물질(1)과 표지 물질로 구성된 추적자가 크로마토그래피적으로 전개될 수 있도록 미리 상기 결합 영역의 상류 구역에 배치되거나 또는 시료 첨가 시에 구가된 크로마토그래피 스트립을 사용하고, 시료 첨가 시에 상기 결합 영역의 상류 구역에 젤라틴을 첨가하거나 또는 추적자가 미리 배치된 영역을 제외한 상기 결합 영역의 상류 구역에 미리 젤라틴을 배치하는 것을 포함하는 검사 방법.A chromatographic carrier is characterized in that the tracking material (2) capable of binding to the analyte is either directly or indirectly fixed through the trapping material (3) capable of binding the analyte and the trapping material (2) A trapping material (4) capable of specifically binding to a trapping material (1) that specifically binds to a substance (2) has one or more engagement areas fixed in a downstream zone of the area to which the trapping material (2) A chromatographic strip previously placed in the upstream region of the binding region or spherical at the time of sample addition is used so that the tracer composed of the substance (1) and the labeling substance can be developed chromatographically, Gelatin is added to the upstream zone of the zone, or gelatin is added to the upstream zone of the joining zone, Test method comprises placing the tin. 제1항에 있어서, 젤라틴 겔이 15℃ 이상에서 용융되는 검사 방법.The method according to claim 1, wherein the gelatin gel is melted at 15 ° C or higher. 제1항에 있어서, 젤라틴 겔이 25℃ 이상에서 용융되는 검사 방법.The method according to claim 1, wherein the gelatin gel is melted at 25 ° C or higher. 제1항, 제2항 또는 제3항에 있어서, 시료가 대변 또는 소변인 검사 방법.The method according to any one of claims 1, 2, and 3, wherein the sample is feces or urine. 제1항에 있어서, 트래핑 물질(1)에 특이적으로 결합 가능한 트래핑 물질(4)이 트래핑 물질(2)이 고정된 영역의 하류 구역에 고정된 영역을 결합 영역으로 사용하고, 트래핑 물질(2)아 고정된 영역과 트래핑 물질(4)이 고정된 영역 사이의 구역에 젤라틴을 첨가 또는 미리 배치한 검사 방법.2. A method according to claim 1, characterized in that a trapping material (4) capable of specifically binding to the trapping material (1) is used as a binding region in which the trapping material (2) ) Gelatin is added or pre-arranged in the region between the sub-fixed region and the fixed region of the trapping material (4). 적어도 제1항의 크로마토그래피 스트립 및 젤라틴 또는 추적자가 미리 배치된 영역을 제외한 제1항의 결합 영역의 상류 구역에 미리 젤라틴이 배치된 젤라틴-배치 크로마토그래피 스트립을 포함하는 검사용 키트.A kit for testing comprising a gelatine batch chromatographic strip having a gelatine pre-positioned in the upstream region of the binding region of claim 1, at least excluding the chromatographic strip of claim 1 and the region pre-arranged with gelatine or tracer. 제6항에 있어서, 젤라틴 겔이 25℃ 이상에서 용융되는 검사용 키트.7. The kit for testing according to claim 6, wherein the gelatin gel is melted at 25 DEG C or higher. 제7항에 있어서, 젤라틴 겔이 15℃ 이상에서 용융되는 검사용 키트.The testing kit according to claim 7, wherein the gelatin gel is melted at 15 ° C or higher. 제6항, 제7항 또는 제8항에 있어서, 시료가 대변 또는 소변인 검사용 키트.The test kit according to claim 6, 7 or 8, wherein the sample is feces or urine. 트래핑 물질(1)에 특이적으로 결합 가능한 트래핑 물질(4)이 트래핑 물질(2)이 고정된 영역의 하류 구역에 고정된 영역을 결합 영역으로 사용하고, 트래핑 물질(2)이 고정된 영역과 트래핑 물질(4)이 고정된 영역 사이의 구역에 젤라틴을 첨가 또는 미리 배치한 제1항에 따른 검사용 키트.A trapping material (4) capable of specifically binding to the trapping material (1) is used as a binding region in which the trapping material (2) is fixed in the downstream region of the region to which the trapping material (2) The kit for testing according to claim 1, wherein gelatin is added or pre-arranged in the area between the areas where trapping material (4) is fixed. ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.※ Note: It is disclosed by the contents of the first application.
KR1019970701223A 1995-06-27 1996-06-26 Inspection kit KR100411406B1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
JP7160610A JPH1138006A (en) 1995-06-27 1995-06-27 Inspection method and inspection kit
JP95/160610 1995-06-27
PCT/JP1996/001769 WO1997001761A1 (en) 1995-06-27 1996-06-26 Assay method and assay kit

Publications (2)

Publication Number Publication Date
KR970705752A true KR970705752A (en) 1997-10-09
KR100411406B1 KR100411406B1 (en) 2004-04-28

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ID=15718665

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Application Number Title Priority Date Filing Date
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JP (1) JPH1138006A (en)
KR (1) KR100411406B1 (en)
CN (1) CN1157040A (en)
AU (1) AU6241996A (en)
BR (1) BR9606500B1 (en)
WO (1) WO1997001761A1 (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR100421346B1 (en) * 1999-06-21 2004-03-06 마츠시타 덴끼 산교 가부시키가이샤 Quantitative chromatographic measuring device and method for manufacturing the same

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP4695316B2 (en) * 1999-07-13 2011-06-08 湧永製薬株式会社 Silver / silver chloride electrode and electrochemical biosensor using the same
EP2077444A3 (en) 2000-09-25 2009-11-04 Panasonic Corporation Chromatography quantitative measuring apparatus
US20030100128A1 (en) * 2000-12-26 2003-05-29 Noriko Kenjyou Specific bonding analysis method and specific bonding analysis device using it
WO2011125877A1 (en) * 2010-03-31 2011-10-13 積水メディカル株式会社 Measurement method using immunochromatography, test strip for immunochromatography, and measurement reagent kit for immunochromatography
JP6048650B2 (en) * 2012-10-31 2016-12-21 住友ベークライト株式会社 Membrane support for detecting sugar chain recognition molecules

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JPS5925184B2 (en) * 1978-12-22 1984-06-15 天野製薬株式会社 Method for eliminating nonspecific inhibitory effects in immunoassays
JPS5642142A (en) * 1979-08-31 1981-04-20 Amano Pharmaceut Co Ltd Removing method for nonspecific reaction inhibiting action in immunity measuring method
JPS6298260A (en) * 1985-10-25 1987-05-07 Nitto Electric Ind Co Ltd Kit for measuring immune active material
CA1303983C (en) * 1987-03-27 1992-06-23 Robert W. Rosenstein Solid phase assay
US5075078A (en) * 1989-10-05 1991-12-24 Abbott Laboratories Self-performing immunochromatographic device
JPH06289025A (en) * 1993-03-31 1994-10-18 Cosmo Sogo Kenkyusho:Kk Nonspecific reaction prevention composition for blocking and solid-phase carrier
JPH07128335A (en) * 1993-10-29 1995-05-19 Tosoh Corp Production of carrier for immunoassay

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR100421346B1 (en) * 1999-06-21 2004-03-06 마츠시타 덴끼 산교 가부시키가이샤 Quantitative chromatographic measuring device and method for manufacturing the same

Also Published As

Publication number Publication date
CN1157040A (en) 1997-08-13
AU6241996A (en) 1997-01-30
BR9606500B1 (en) 2009-01-13
BR9606500A (en) 1998-07-14
JPH1138006A (en) 1999-02-12
WO1997001761A1 (en) 1997-01-16
KR100411406B1 (en) 2004-04-28

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