KR970701260A - Extracorporeal Cell Culture and Transplantation Kits - Google Patents

Abstract

The present invention provides a method for selecting cells having a desired phenotype from cell mixtures obtained from the human body; Means for separating selected cells from the mixture; Means for culturing the isolated cells; Contains an effective amount of cell expansion factor selected from the group consisting of GM-CSF, G-CSF, IL-1, IL-3, IL-6, TPO, EPO, flt3-ligand, SF and GM-CSF / IL-3 fusion proteins Composition to be; And an in vitro hepatocyte culture and transplant kit comprising a cell proliferation medium.

Description

Extracorporeal Cell Culture and Transplantation Kits

Since this is an open matter, no full text was included.

Claims (15)

a) means for selecting a cell having a desired phenotype from a cell mixture obtained from the human body; b) means for separating the selected cells from the mixture; c) means for culturing the isolated cells; d) cell expansion factors selected from the group consisting of GM-CSF, G-CSF, IL-1, IL-3, IL-6, TPO, EPO, EPO flt3-ligand, SF and GM-CSF / IL-3 fusion proteins Compositions containing an effective amount; And e) cell proliferation medium; The separating means is adapted to receive a mixture of cells and selection means and is adapted to separate the selected cells from the mixture; The culture means is adapted to receive the isolated cells from the separation means, the cell proliferation medium and the expansion factor composition, further contacting the expansion factor with the separated cells sufficient to allow cellular expansion of the separated cells. In vitro cell culture and transplant kit adapted to allow. The kit of claim 1, wherein the isolated cells are human hematopoietic liver or progenitor cells. The kit of claim 1 further comprising a container for first containing a mixture of cells collected from the human body. The monoclonal antibody of claim 2, wherein the means for selecting hematopoietic liver or progenitor cells binds to a cell marker selected from the group consisting of a) flt3 receptor binding protein and b) CD34, Try-1 or My-10. Kit containing. The kit of claim 2, wherein the means for selecting hematopoietic liver or progenitor cells contains a metabolic antagonist and a dietary factor selected from the group consisting of SF and flt3-ligand. The kit of claim 1 containing the composition GM-CSF / IL-3 fusion protein. The kit of claim 1, wherein the composition contains IL-1α. The kit of claim 1 wherein the composition contains flt3-ligand. The kit of claim 1 wherein the composition contains GM-CSF. The kit of claim 1, wherein the composition contains flt3-L and one of an IL-3 or GM-CSF / IL-3 fusion protein. a) means for selecting a cell having a desired phenotype from a cell mixture; b) means for separating the selected cells from the mixture; c) means for culturing the isolated cells; d) a composition containing an effective amount of cell expansion factor selected from the group consisting of G-CSF, IL-3, IL-6, TPO, EPO and SF; And e) cell proliferation medium; The separating means is adapted to receive a mixture of cells and selection means and is adapted to separate the selected cells from the mixture; The culture means is adapted to receive the isolated cells from the separation means, cell proliferation medium, and expansion factor composition, and further adapted to allow contact of the expansion factor with sufficient separated cells to expand the cells. In vitro cell culture and transplant kit. The kit of claim 11, wherein the isolated cells are human hematopoietic liver or progenitor cells. The kit of claim 11 wherein the means for selecting hematopoietic liver or progenitor cells contains a monoclonal antibody that binds to a cell marker selected from the group consisting of CD34, Try-1 or My-10. The kit of claim 11 wherein the means for selecting hematopoietic liver or progenitor cells contains a metabolic antagonist and a dietary factor selected from the group consisting of SF and flt3-ligand. The kit of claim 11 further comprising a container for containing a mixture of cells collected from the human body. ※ Note: The disclosure is based on the initial application.