KR970070190A - Maltooligosaccharide-producing alpha-amylase and a strain producing it - Google Patents

Maltooligosaccharide-producing alpha-amylase and a strain producing it Download PDF

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Publication number
KR970070190A
KR970070190A KR1019960011992A KR19960011992A KR970070190A KR 970070190 A KR970070190 A KR 970070190A KR 1019960011992 A KR1019960011992 A KR 1019960011992A KR 19960011992 A KR19960011992 A KR 19960011992A KR 970070190 A KR970070190 A KR 970070190A
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South Korea
Prior art keywords
amylase
producing
starch
maltooligosaccharide
strain
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KR1019960011992A
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Korean (ko)
Inventor
윤상현
김묘정
권기성
김정완
이인원
박관화
김정원
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박관화
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Priority to KR1019960011992A priority Critical patent/KR970070190A/en
Publication of KR970070190A publication Critical patent/KR970070190A/en

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Abstract

본 발명은 수탁번호 KFCC-10895인 Bacillus subtilis YSH 및 그것으로부터 분리한, 전분을 기질로 할 때 말토오스 및 말토트리오스를 주로 생성하는 중간내열성 α-아밀라제에 관한 것이다. 본 발명의 효소는 말토올리고당을 생산하는데 유용하며, 또한 최적온도가 60-65℃ 이고, 75℃ 이상에서는 거의 실활되므로 전분식품에 직접 첨가함으로써 전분식품의 노화를 억제할 수 있다.The present invention relates to Bacillus subtilis YSH, accession number KFCC-10895, and an intermediate heat-resistant? -Amylase which mainly produces maltose and maltotriose when starch is separated from the starch. The enzyme of the present invention is useful for the production of maltooligosaccharide and can be inhibited from aging of starch food by adding it directly to the starch food because the optimum temperature is 60-65 ° C and it is almost inactivated at 75 ° C or higher.

Description

말토올리고당 생성 알파-아밀라제 및 그것을 생산하는 균주Maltooligosaccharide-producing alpha-amylase and a strain producing it

본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음Since this is a trivial issue, I did not include the contents of the text.

제1도는 본 발명 α-아밀라제의 분자량을 측정하기 위한 SDS-PAGE사진이다. 제2도는 본 발명 α-아밀라제의 등전점을 측정하기 위한 IEF 사진이다. 제3도는 본 발명 α-아밀라제의 온도특성 곡선이다.FIG. 1 is an SDS-PAGE photograph for measuring the molecular weight of the .alpha.-amylase of the present invention. FIG. 2 is an IEF photograph for measuring the isoelectric point of? -Amylase of the present invention. FIG. 3 is a temperature characteristic curve of? -Amylase of the present invention. FIG.

Claims (5)

수탁번호 KFC-10895인 Bacillus subtilis YSH.Accession No. KFC-10895, Bacillus subtilis YSH. 제1항의 균주에서 분리한 α-아밀라제.A? -Amylase isolated from the strain of claim 1. 제2항에 있어서, 상기 α-아밀라제가 전분을 기질로 하였을 때 말토오스와 말토트리오스를 총 분해산물의 40% 이상 생성하는 α-아밀라제.The α-amylase according to claim 2, wherein the α-amylase produces maltose and maltotriose in an amount of 40% or more of total decomposition products when the α-amylase is starch. 제2항에 있어서, 상기 α-아밀라제가 SDS-PAGE로 측정한 분자량이 약 63.6kD이고, 등전점이 약 5.8인 α-아밀라제.The α-amylase according to claim 2, wherein the α-amylase has a molecular weight of about 63.6 kD as measured by SDS-PAGE and an isoelectric point of about 5.8. 제2항에 있어서, 상기 α-아밀라제가 최적 온도 범위가 60-65℃인 α-아밀라제.The α-amylase according to claim 2, wherein the α-amylase has an optimum temperature range of 60-65 ° C. ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.※ Note: It is disclosed by the contents of the first application.
KR1019960011992A 1996-04-19 1996-04-19 Maltooligosaccharide-producing alpha-amylase and a strain producing it KR970070190A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
KR1019960011992A KR970070190A (en) 1996-04-19 1996-04-19 Maltooligosaccharide-producing alpha-amylase and a strain producing it

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
KR1019960011992A KR970070190A (en) 1996-04-19 1996-04-19 Maltooligosaccharide-producing alpha-amylase and a strain producing it

Publications (1)

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KR970070190A true KR970070190A (en) 1997-11-07

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR100625100B1 (en) * 2002-07-15 2006-09-19 주식회사 제노포커스 Maltopentaose-Producing Amylase and Process for Producing Maltopentaose
KR20160125619A (en) 2015-04-22 2016-11-01 동아대학교 산학협력단 Maltotrios-Producing Amylase derived from Microbulbifer sp. and the Process for Producing Maltotrios therewith

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR100625100B1 (en) * 2002-07-15 2006-09-19 주식회사 제노포커스 Maltopentaose-Producing Amylase and Process for Producing Maltopentaose
KR20160125619A (en) 2015-04-22 2016-11-01 동아대학교 산학협력단 Maltotrios-Producing Amylase derived from Microbulbifer sp. and the Process for Producing Maltotrios therewith

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