KR970062034A - Mutant proteolytic enzymes resistant to human immunodeficiency virus type 1 protease inhibitors - Google Patents

Mutant proteolytic enzymes resistant to human immunodeficiency virus type 1 protease inhibitors Download PDF

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Publication number
KR970062034A
KR970062034A KR1019960004158A KR19960004158A KR970062034A KR 970062034 A KR970062034 A KR 970062034A KR 1019960004158 A KR1019960004158 A KR 1019960004158A KR 19960004158 A KR19960004158 A KR 19960004158A KR 970062034 A KR970062034 A KR 970062034A
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KR
South Korea
Prior art keywords
immunodeficiency virus
human immunodeficiency
virus type
hiv
protease inhibitors
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KR1019960004158A
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Korean (ko)
Inventor
권영도
이태규
김성천
윤홍식
Original Assignee
성재갑
주식회사 Lg 화학
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Priority to KR1019960004158A priority Critical patent/KR970062034A/en
Publication of KR970062034A publication Critical patent/KR970062034A/en

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Abstract

본 발명은 사람 면역결핍 바이러스 1형(Human Immunodeficiency Virus type 1 : 이하 HIV-1)의 단백질 분해요소 억제제인 LB-71262에 대하여 내성을 가지는 가지는 HIV-1 변이체에서 확인한 돌연변이 단백질 분해요소에 관한 것으로, 특히 HIV-1의 단백질 분해요소의 45번 아미노산인 이소류신이 메티오닌으로 치환된 것(154M)을 예로 들 수 있다.The present invention relates to a mutant proteolytic element identified in HIV-1 mutants having resistance to LB-71262, a proteolytic element inhibitor of human immunodeficiency virus type 1 (hereinafter referred to as HIV-1) In particular, the case of isoleucine, the 45th amino acid of the proteolytic element of HIV-1, is replaced by methionine (154M).

Description

사람면역결핍 바이러스 1형의 단백질 분해요소 억제제에 대해 내성을 갖는 돌연변이 단백질 분해요소Mutant protein degradation factor resistant to proteolytic element inhibitors of human immunodeficiency virus type 1

본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음Since this is a trivial issue, I did not include the contents of the text.

제1도는 HIV-1를 MT-2 세포에 감염시킨 후 HIV-1 단백질 분해요소 억제제인 LB-71262의 농도를 점차 높여가면서 계대배양하여, 19계대에서 감염된 MT-2세포로부터 얻은 게놈DNA 및 이의 PCR산물을 전기영동한 결과이다.FIG. 1 shows the results of infection of MT-2 cells with HIV-1, followed by subculture with increasing concentrations of LB-71262, an HIV-1 proteolytic element inhibitor, to elucidate genomic DNA obtained from infected MT- PCR products were electrophoresed.

제2도는 HIV-1를 MT-2 세포에 감염시킨 후 HIV-1 단백질 분해요소 억제제인 LB-71262의 농도를 점차 높여가면서 계대배양하여, 19계대에서 발생한 HIV-1 단백질 분해요소 유전자에서의 돌연변이를 나타낸 것이다.Figure 2 shows the results of transfection of HIV-1 with MT-2 cells and subsequent passage of LB-71262, an inhibitor of the HIV-1 proteolytic element, in mutant strains of HIV-1 proteolytic elements .

Claims (3)

사람 면역결핍 바이러스 1형(HIV-1)의 단백질 분해요소 억제제인 하기 구조시의 화합물에 대하여 내성을 갖는 돌연변이 단백질 분해요소.A mutant proteolytic element that is resistant to compounds in the following structure which is a proteolytic element inhibitor of human immunodeficiency virus type 1 (HIV-1). 제1항에 있어서, 상기 HIV-1의 단백질 분해요소의 54번 아미노산인 이소류신이 메틴오닌으로 치환된것(154M).The method according to claim 1, wherein the isoleucine, amino acid 54 of the proteolytic element of HIV-1, is replaced with methionine (154M). 제1항의 돌연변이 단백질 분해요소를 코딩하는 유전자.A gene encoding the mutant proteolytic element of claim 1. ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.※ Note: It is disclosed by the contents of the first application.
KR1019960004158A 1996-02-22 1996-02-22 Mutant proteolytic enzymes resistant to human immunodeficiency virus type 1 protease inhibitors KR970062034A (en)

Priority Applications (1)

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KR1019960004158A KR970062034A (en) 1996-02-22 1996-02-22 Mutant proteolytic enzymes resistant to human immunodeficiency virus type 1 protease inhibitors

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KR1019960004158A KR970062034A (en) 1996-02-22 1996-02-22 Mutant proteolytic enzymes resistant to human immunodeficiency virus type 1 protease inhibitors

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KR970062034A true KR970062034A (en) 1997-09-12

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Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR0125117B1 (en) * 1994-06-15 1997-12-05 성재갑 Novel hiv protease inhibitors with anti aids effect and the process for preparation thereof

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR0125117B1 (en) * 1994-06-15 1997-12-05 성재갑 Novel hiv protease inhibitors with anti aids effect and the process for preparation thereof

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
AIDS Res Hum Retroviruses 1995 Jun;11(6):671-6 *
J Virol 1995 Apr;69(4):2148-52 *
J Virol 1995 Feb;69(2):701-6 *
Proc Natl Acad Sci U S A 1993 Aug 15;90(16):7543-7 *

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