KR970061918A - Purification of Genes Recombinant Chicken Growth Hormone Expressed in Escherichia coli - Google Patents

Purification of Genes Recombinant Chicken Growth Hormone Expressed in Escherichia coli Download PDF

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Publication number
KR970061918A
KR970061918A KR1019960004156A KR19960004156A KR970061918A KR 970061918 A KR970061918 A KR 970061918A KR 1019960004156 A KR1019960004156 A KR 1019960004156A KR 19960004156 A KR19960004156 A KR 19960004156A KR 970061918 A KR970061918 A KR 970061918A
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KR
South Korea
Prior art keywords
growth hormone
escherichia coli
chicken growth
inclusion body
purification
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KR1019960004156A
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Korean (ko)
Inventor
박천길
이홍균
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성재갑
주식회사 Lg 화학
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Priority to KR1019960004156A priority Critical patent/KR970061918A/en
Publication of KR970061918A publication Critical patent/KR970061918A/en

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Abstract

본 발명은 대장균에서 발현된 유전자 재조합 닭 성장 호르몬의 정제 방법에 관한 것으로, 닭 성장 호르몬이 봉입체로 발현된 대장균을 파쇄하여 봉입체를 수득하고, 상기 봉입체를 세척하고, 강알칼리와 요소로 용해하고 산화시킨 후, 한외여과 및 음이온 교환 크로마토그래피함으로써 재조합 닭 성장 호르몬을 고순도로 효율적으로 정제할 수 있다.The present invention relates to a method for purifying recombinant chicken growth hormone expressed in Escherichia coli, which comprises disrupting Escherichia coli expressed with an inclusion body of a chicken growth hormone to obtain an inclusion body, washing the inclusion body, dissolving it with a strong alkali and urea, After that, the recombinant chicken growth hormone can be efficiently purified with high purity by ultrafiltration and anion exchange chromatography.

Description

대장균에서 발현된 유전자 재조합 닭 성장 호르몬의 정제방법Purification of Genes Recombinant Chicken Growth Hormone Expressed in Escherichia coli

본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음Since this is a trivial issue, I did not include the contents of the text.

제1도는 본 발명에 따른 정제 과정의 흐름도이다.FIG. 1 is a flow chart of a purification process according to the present invention.

Claims (6)

닭 성장 호르몬이 봉입체로 발현된 대장균을 파쇄하여 봉입체를 수득하고, 상기 봉입체를 세척하고, 강알칼리와 요소로 용해하고 산화시킨 후, 한외여과 및 음이온 교환 크로마토그래피하는 단계를 포함하는 대장균에서 발현된 닭 성장 호르몬의 정제 방법.The present invention provides a method for producing chickens, the method comprising: disrupting Escherichia coli expressed in an enclosure of chicken growth hormone to obtain an inclusion body; washing the inclusion body; dissolving and oxidizing the inclusion body with strong alkali and urea, followed by ultrafiltration and anion exchange chromatography; Method of purifying growth hormone. 제1항에 있어서, 상기 강알칼리가 수산화나트륨인 것을 특징으로 하는 방법.The method of claim 1, wherein the strong alkali is sodium hydroxide. 제2항에 있어서, 강알카리를 가하여 단백질 용액의 pH를 11이상으로 조정하는 것을 특징으로 하는 방법.The method according to claim 2, wherein the pH of the protein solution is adjusted to 11 or more by adding strong alkali. 제1항에 있어서, 상기 요소의 농도가 4 내지 8M인 것을 특징으로 하는 방법.The method of claim 1, wherein the concentration of the element is between 4 and 8 M. 제1항에 있어서, 상기 한외여과가 글리신을 포함하는 완충용액으로 수행되는 것을 특징으로 하는 방법.The method of claim 1, wherein the ultrafiltration is performed with a buffer solution comprising glycine. 제1항에 있어서, 상기 음이온 교환 크로마토그래피에서 글리신을 포함하는 0.0 내지 0.2M 선형 농도 구배의 염화나트륨 완충용액으로 닭 성장 호르몬 단백질을 용출시키는 것을 특징으로 하는 방법.3. The method of claim 1, wherein the anion exchange chromatography elutes the chicken growth hormone protein with a sodium chloride buffer solution of 0.0 to 0.2M linear gradient of concentration containing glycine. ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.※ Note: It is disclosed by the contents of the first application.
KR1019960004156A 1996-02-22 1996-02-22 Purification of Genes Recombinant Chicken Growth Hormone Expressed in Escherichia coli KR970061918A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
KR1019960004156A KR970061918A (en) 1996-02-22 1996-02-22 Purification of Genes Recombinant Chicken Growth Hormone Expressed in Escherichia coli

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Application Number Priority Date Filing Date Title
KR1019960004156A KR970061918A (en) 1996-02-22 1996-02-22 Purification of Genes Recombinant Chicken Growth Hormone Expressed in Escherichia coli

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KR970061918A true KR970061918A (en) 1997-09-12

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Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0173215A2 (en) * 1984-08-27 1986-03-05 Bio-Technology General Corporation Method for recovering purified growth hormones from genetically engineered microorganisms
US4997917A (en) * 1984-03-08 1991-03-05 Ciba-Geigy Corporation Preparation of 1:2 chromium complex azo dyes asymmetrically substituted with solubilizing

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4997917A (en) * 1984-03-08 1991-03-05 Ciba-Geigy Corporation Preparation of 1:2 chromium complex azo dyes asymmetrically substituted with solubilizing
EP0173215A2 (en) * 1984-08-27 1986-03-05 Bio-Technology General Corporation Method for recovering purified growth hormones from genetically engineered microorganisms

Non-Patent Citations (6)

* Cited by examiner, † Cited by third party
Title
Biochim Biophys Acta. 1988 Feb 28;949(2):247-51 *
Gen Comp Endocrinol. 1984 Dec;56(3):389-400 *
Gen Comp Endocrinol. 1989 Nov;76(2):330-9 *
J Endocrinol. 1977 May;73(2):321-9 *
J Endocrinol. 1988 Sep;118(3):381-7 *
J Endocrinol. 1990 May;125(2):207-15 *

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