KR970061918A - Purification of Genes Recombinant Chicken Growth Hormone Expressed in Escherichia coli - Google Patents
Purification of Genes Recombinant Chicken Growth Hormone Expressed in Escherichia coli Download PDFInfo
- Publication number
- KR970061918A KR970061918A KR1019960004156A KR19960004156A KR970061918A KR 970061918 A KR970061918 A KR 970061918A KR 1019960004156 A KR1019960004156 A KR 1019960004156A KR 19960004156 A KR19960004156 A KR 19960004156A KR 970061918 A KR970061918 A KR 970061918A
- Authority
- KR
- South Korea
- Prior art keywords
- growth hormone
- escherichia coli
- chicken growth
- inclusion body
- purification
- Prior art date
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- Peptides Or Proteins (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
본 발명은 대장균에서 발현된 유전자 재조합 닭 성장 호르몬의 정제 방법에 관한 것으로, 닭 성장 호르몬이 봉입체로 발현된 대장균을 파쇄하여 봉입체를 수득하고, 상기 봉입체를 세척하고, 강알칼리와 요소로 용해하고 산화시킨 후, 한외여과 및 음이온 교환 크로마토그래피함으로써 재조합 닭 성장 호르몬을 고순도로 효율적으로 정제할 수 있다.The present invention relates to a method for purifying recombinant chicken growth hormone expressed in Escherichia coli, which comprises disrupting Escherichia coli expressed with an inclusion body of a chicken growth hormone to obtain an inclusion body, washing the inclusion body, dissolving it with a strong alkali and urea, After that, the recombinant chicken growth hormone can be efficiently purified with high purity by ultrafiltration and anion exchange chromatography.
Description
본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음Since this is a trivial issue, I did not include the contents of the text.
제1도는 본 발명에 따른 정제 과정의 흐름도이다.FIG. 1 is a flow chart of a purification process according to the present invention.
Claims (6)
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
KR1019960004156A KR970061918A (en) | 1996-02-22 | 1996-02-22 | Purification of Genes Recombinant Chicken Growth Hormone Expressed in Escherichia coli |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
KR1019960004156A KR970061918A (en) | 1996-02-22 | 1996-02-22 | Purification of Genes Recombinant Chicken Growth Hormone Expressed in Escherichia coli |
Publications (1)
Publication Number | Publication Date |
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KR970061918A true KR970061918A (en) | 1997-09-12 |
Family
ID=66221747
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
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KR1019960004156A KR970061918A (en) | 1996-02-22 | 1996-02-22 | Purification of Genes Recombinant Chicken Growth Hormone Expressed in Escherichia coli |
Country Status (1)
Country | Link |
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KR (1) | KR970061918A (en) |
Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP0173215A2 (en) * | 1984-08-27 | 1986-03-05 | Bio-Technology General Corporation | Method for recovering purified growth hormones from genetically engineered microorganisms |
US4997917A (en) * | 1984-03-08 | 1991-03-05 | Ciba-Geigy Corporation | Preparation of 1:2 chromium complex azo dyes asymmetrically substituted with solubilizing |
-
1996
- 1996-02-22 KR KR1019960004156A patent/KR970061918A/en not_active Application Discontinuation
Patent Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4997917A (en) * | 1984-03-08 | 1991-03-05 | Ciba-Geigy Corporation | Preparation of 1:2 chromium complex azo dyes asymmetrically substituted with solubilizing |
EP0173215A2 (en) * | 1984-08-27 | 1986-03-05 | Bio-Technology General Corporation | Method for recovering purified growth hormones from genetically engineered microorganisms |
Non-Patent Citations (6)
Title |
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Biochim Biophys Acta. 1988 Feb 28;949(2):247-51 * |
Gen Comp Endocrinol. 1984 Dec;56(3):389-400 * |
Gen Comp Endocrinol. 1989 Nov;76(2):330-9 * |
J Endocrinol. 1977 May;73(2):321-9 * |
J Endocrinol. 1988 Sep;118(3):381-7 * |
J Endocrinol. 1990 May;125(2):207-15 * |
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